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All Journal HAYATI Journal of Biosciences Jurnal Gizi dan Pangan Jurnal Teknologi Dan Industri Pangan Jurnal Pengolahan Hasil Perikanan Indonesia VALENSI ILMU KELAUTAN: Indonesian Journal of Marine Sciences JURNAL KIMIA SAINS DAN APLIKASI Current Biochemistry Indonesian Journal of Human Nutrition Journal of Mathematical and Fundamental Sciences Indonesian Journal of Pharmaceutical Science and Technology Jurnal Sumberdaya Hayati Warta IHP (Warta Industri Hasil Pertanian) ALCHEMY Jurnal Penelitian Kimia Chemistry Indonesian Journal of Chemical Research QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Ilmu dan Teknologi Kayu Tropis Jurnal Farmamedika (Pharmamedica Journal) Tunas Medika Jurnal Kedokteran & Kesehatan Indonesian Journal of Applied Research (IJAR) Wood Research Journal : Journal of Indonesian Wood Research Society Wood Research Journal : Journal of Indonesian Wood Research Society Journal of Food Technology and Health Journal of Fisheries & Marine Halal Studies and Society
Mega Safithri
Departemen Biokimia, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor Jl. Raya Dramaga Kampus IPB Dramaga Bogor, Jawa Barat 16680 Indonesia
Religion Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Toxicity Extract and Faction of Surian Toona sinensis Leaf and Bark against Shrimp Larvae Artemia salina L. Rori Theresia; Syamsul Falah; Mega Safithri; Muhammad Assyar
Current Biochemistry Vol. 3 No. 3 (2016)
Publisher : IPB University

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Abstract

Surian (Toona sinensis) is one of the herbs that contain many bioactive compounds. Part of surian plants that can be utilized as therapy is leaf and bark. This study aims to determine the toxicity of leaf and bark extract of Surian through Bhrine Shrimp Lethality Test (BSLT), then compare toxic concentration of water extract, and ethanol 70 %, and n-hexane, diethyl ether and ethyl acetate fractions to shrimp larvae (Artemia salina L). The results showed that water content of leaves simplicia was 8.08 % and bark was 6.26 %. The highest extract rendement value by ethanol solvent was 23 %. The result of BSLT test probit analysis showed that the extract of ethyl acetate bark fraction had the highest toxicity with LC50 value 405.641 ppm, while leaf water extract had the lowest toxicity with LC50 value 707.787 ppm.
Antibacterial activity of Stichopus hermanii and Stichopus variegatus methanol extract Hendra Susanto; Mega Safithri; Kustiariyah Tarman
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

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Abstract

Since 500 years ago sea cucumber has been used as wound healing medicine. Sea cucumbers are thought to function as a cell growth factor and have the potential as an antibacterial agent. This study aims to determine whether the methanol extract of sea cucumber has antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa so that it can be used to help diabetic wound healing of people infected with the same bacteria. The extraction method used was maceration with methanol to dried frozen simplicia. The simplicia was obtained by freeze drying to liquid preparation of heated fresh sea cucumber. The antibacterial activity test was performed using well diffusion method. There were no any antibacterial activity found in the two methanol extracts of sea cucumber. Keywords: antibacterial agent, Stichopus hermanii, Stichopus variegatus
Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Oczhinvia Dwitasari; Djarot Sasongko Hami Seno; Mega Safithri
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

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Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment
Antibacterial Activity of Ethanol Extract of Spirulina platensis Prayoga Pannindrya; Mega Safithri; Kustiariyah Tarman
Current Biochemistry Vol. 7 No. 2 (2020)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.7.1.1

Abstract

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.
Molecular Docking of Active Compounds Piper crocatum on the A-Glucosidase Enzyme as Antidiabetic Mustika Weni; Mega Safithri; Djarot Sasongko Hami Seno
Indonesian Journal of Pharmaceutical Science and Technology Vol 7, No 2 (2020)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/ijpst.v7i2.21120

Abstract

Ethanol extract of Piper crocatum leaves has inhibitory activity of α-glucosidase enzyme. Ethyl acetate fraction from Piper crocatum leaves has the highest antioxidant activity. Previous research has provided information that the ethyl acetate fraction of Piper crocatum leaves has an inhibition of α-glucosidase containing 6XO32ZSP1D, Ethyl L-serinate hydrochloride compound, Schisandrin B compound, Columbin compound, 4- (4-methoxy-phenylamino) -2 compound, 3-dihydro-1H-4a, 9-diazacyclopenta (b) fluorine-10-carbonitrile, compound 6-Amino-4- [3- (benzyloxy) phenyl] -3-tert-butyl-2,4-dihydropyrano [2, 3-c] pyrazole-5-carbonitrile, compound 4 - {{4.6-Bis [(3R, 5S) -3,5-diamino-1-piperydinyl] -1,3,5-triazine-2-yl} amino) benzenesulfonamide and compound 1.1 '- (1,4-butanediyl) bis {2,6-dimethyl-4 - [(3-methyl-1,3-benzothiazol-2 (3H) ylidene) methyl] pyridinium. This study aims to study the interaction between bioactive compounds contained in ethyl acetate fraction of Piper crocatum leaves with α-glucosidase enzyme in In Silico using AutoDock Vina, Columbin shows the lowest binding energy with binding sites with amino acids Ser240, Asp242, His280, Arg315, Glu411, Phe159, Arg442, Tyr158 and Phe303. Columbin has the stability and inhibits the α-glucosidase enzyme from S. cerevisiae better than the seven other compounds, because it has OH and CH3 groups which play a role in the interaction with around the active side of the α-glucosidase enzyme.Keywords: Columbin, In Silico, α-Glucosidase
Aktivitas Penurunan Kadar Glukosa pada Tikus yang Diinduksi Aloksan dari Ekstrak Air Angkak, Bekatul, dan Kombinasinya Hasim Hasim; Didah Nur Faridah; Mega Safithri; Husnawati Husnawati; Agus Setiyono; Hanif Alamudin Manshur
Warta Industri Hasil Pertanian Vol 37, No 2 (2020)
Publisher : Balai Besar Industri Agro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32765/wartaihp.v37i2.5460

Abstract

ABSTRAK: Angkak dan bekatul mengandung komponen bioaktif sebagai antihiperglikemik. Namun penelitian mengenai aktivitas antihiperglikemik angkak dan bekatul secara in vivo belum tersedia. Tujuan penelitian ini adalah menguji efektivitas pemberian ekstrak air angkak, ekstrak air bekatul serta kombinasinya dalam proses penurunan jumlah glukosa dalam darah tikus (Rattus norvegicus) strain Sprague Dawley yang diinduksi aloksan. Penelitian dilakukan dengan melakukan preparasi sampel yaitu ekstraksi air angkak dan bekatul, pengkondisian hewan uji yaitu tikus (Rattus norvegicus) strain Sprague Dawley dengan induksi aloksan, pembagian tikus menjadi sembilan kelompok. Hasil penelitian menunjukkan penurunan kadar glukosa darah tikus paling rendah adalah pada tikus kelompok V (angkak 2) dengan pemberian ekstrak air angkak 100 mg/Kg bb, dengan penurunan 61,43% lebih tinggi dibandingkan dengan kelompok III, tikus yang diberi obat glibenklamid yang  turun sebesar 40,88%. Pada kelompok V, kadar glukosa darah pada hari ke-3 sebesar 256,67±174,77 mg/dL dan turun pada hari ke-12 hingga mencapai glukosa darah normal sebesar 99,00±9,64 mg/dL. Penurunan kadar glukosa darah tikus baik kelompok pemberian ekstrak bekatul maupun dengan kombinasi ekstrak air angkak dan ekstrak air bekatul tidak menunjukkan penurunan kadar glukosa darah yang signifikan.Kata kunci: aloksan, angkak, bekatul, glukosa darahABSTRACT: Bioactive compounds in red yeast rice (traditionally known as angkak) and rice bran are scientifically evidenced to exert antihyperglycemic properties. Nevertheless, in vivo studies on antihyperglycemic activity of red yeast rice and rice bran have not  been reported. This current work aimed to evaluate glucose-lowering effects on alloxan-induced rats (Rattus norvegicus) strain Sprague Dawley treated with water extract of angkak, rice bran and combination of both extracts. The research was first carried out by extraction of RYR and RB, acclimatization of rats induced by alloxan, then separating them into 9 groups. The results showed that the lowest decrease in blood glucose, i.e. 61,43%, was attributed to group 5, namely rats administered with water extract of angkak (100 mg/Kg bw), being much higher compared to group 3 (rats treated with glibenclamide) reaching up to 40,88%. In terms of group 5, the level of blood glucose exhibited a gradual attenuation, from 256,67±174,77 mg/dL on day-3 to 99,00±9,64 mg/dL on day-12, which is a normal blood glucose concentration. It is noteworthy that administration of rice bran extract and combined treatment showed no significant effects on reduction of blood glucose.Keywords:  alloxan, blood glucose, red yeast rice, rice bran, antihyperglycemic
Antihyperglycemic Activity of Piper crocatum Leaves and Cinnamomum burmannii Bark Mixture Extract in Streptozotocin-induced Diabetic Rats Mega Safithri Hasibuan; Sedarnawati Yasni; Maria Bintang; Anna Setiadi Ranti
Journal of Mathematical and Fundamental Sciences Vol. 48 No. 2 (2016)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2016.48.2.8

Abstract

Indonesia presently has the fourth largest diabetic mellitus prevalence of all countries in the world. In a previous study, a mixture extract of Piper crocatum leaves and Cinnamomum burmannii bark showed in vitro antihyperglycemic activity. It acted as inhibitor of the a-glucosidase enzyme and had no toxic effect when it was administered orally to male and female rats for 28 days. In the present study, mixture extracts of P. crocatum leaves and C. burmannii bark were used to observe antihyperglycemic activity in diabetic rats induced with streptozotocin. Mixture extracts of P. crocatum leaves and C. burmannii bark were orally given to diabetic Sprague Dawley rats at various doses for 16 days. The results showed that the treatment led to a reduction of the blood glucose level, an increase in blood insulin level up to 170.75% at 1260 mg/kg body weight, maintaining the blood lipid level of the diabetic rats at a normal level, and an increase of pancreatic ß cells in the islets of Langerhans up to 2.2-fold at 1260 mg/kg body weight. The mixture extracts of P. crocatum and C. burmannii have antihyperglycemic activity, which enhances the number of pancreatic β cells.
In Silico, to Determine The Active Compounds of Black Tea and Turmeric In Increasing The Activity of The Enzyme Sod Akhmad Endang Zainal Hasan; Mega Safithri; Aziz Syamsul Huda; Rini Kurniasih
Indonesian Journal of Applied Research (IJAR) Vol. 3 No. 1 (2022): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v3i1.187

Abstract

Damage to cells caused by stress can be reduced by the presence of antioxidants, one of which is Superoxide dismutase (SOD). The role of the active ingredients of black tea and turmeric will be studied using the in-silico method to identify the active compounds as components in SOD activation. The bioavailability and toxicity of the active compounds of black tea and turmeric were studied and followed by molecular docking and virtual games. The parameters studied are Gibbs's free energy (∆G) and binding site similarity (BSS). The results were analyzed using Gibbs's free energy (∆G) and binding site similarity (BSS) parameters. It was found that those that could increase the activity of Cu/Zn SOD enzymes were Epicatechin gallate (black tea) and curcumin (turmeric), with values ​​of -9.5 and -7.4 Kcal/mol and the same BSS value of 81.8%. The control ligand used was beta amyrin. According to Lipinski's rules, Epicatechin gallate and curcumin compounds can be absorbed well and are safe for consumption. This study concludes that Epicatechin gallate, an active compound of black tea, and curcumin, an active compound of turmeric rhizome, have the best potential to increase the activity of Cu/Zn SOD enzymes based on the results of virtual screening and molecular docking. Epicatechin gallate and curcumin are predicted to be well absorbed by the body because they qualify Lipinski's rules and are not toxic and safe for consumption.
Potensi Ekstrak Tanin Daun dan Kulit Batang Surian sebagai Penghambat α-Glukosidase (Tannin Extract Potential of Surian Leafand Bark as α-Glucosidase Inhibitor) Fitriana S Monisa; Maria Bintang; Mega Safithri; Syamsul Falah
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 14, No 2 (2016): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.764 KB) | DOI: 10.51850/jitkt.v14i2.331

Abstract

The purpose of the study was to know the inhibition activity surian leaves and bark tannin to α-glucosidase by in vitro method. Leaves and bark of surian was extracted with water and ethanol 70% solvent using infundation and maceration methods. Tannin was tested with phytochemical analysis and activity of inhibition to α-glucosidase with a microplate reader at a wavelength of 410 nm. The yield of tannins extract from leaves sample were 65.46% and 66.12% by water and ethanol 70% solvents, respectively, as well as 83.34% and 90.29% were obtained from bark sample. Water and ethanol extract of surian leaves and bark contain flavonoids, triterpenoids, tannins and saponins. Leaves tannin consist mainly hydrolyzable tannin, i.e. galat and gallotanin/ellagotanin, however, bark tannin consist of condensed tannin (catechol). Total tannin content of ethanol and water extract were 9.35 and 7.61 mg g-1 for bark sample, and 6.24 and 5.20 mg g-1 for leaves sample. Inhibition activity test showed that ethanol extract of leaves sample exhibited the highest inhibition activity (IC50=50.44 μg ml-1) and water extract of leaves sample was smallest (IC50=183.22 μg ml-1).
Antibacterial Activity of Ethanol Extract of Red Betel Leaves (Piper crocatum) and Its Fractions against Escherichia coli pBR322 Fernanda Chairunisa; Mega Safithri; Maria Bintang
Current Biochemistry Vol. 9 No. 1 (2022)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.9.1.1

Abstract

Red betel leaf (Piper crocatum) is one of the typical Indonesian herbal plants that has an antibacterial function. This study aimed to test and measure the activity of 70% ethanol extract of red betel leaf and its fractions (n-hexane, ethyl acetate, and water fractions) as antibacterial against specific bacteria Escherichia coli pBR322 based on activity and minimum inhibitory concentration. Extraction was carried out using the maceration method and continued with fractionation based on the level of solvent polarity. Antibacterial activity was indicated by the presence of inhibition zones. The minimum inhibitory concentration was indicated by the appearance of the inhibition zone at the minimum concentration. Results showed that extraction process resulted in crude extract yield of 19.49%. The best antibacterial activity was shown by 1000 ppm n-hexane fraction with an inhibition zone of 2.40 mm ± 0.14. The best minimum inhibitory concentration was 100 ppm n-hexane fraction with an inhibition zone of 0.60 mm ± 0.56.
Co-Authors . SURYANI Abdul Aziz Achyar, Catellia Auliany Agus Setiyono Akhmad Endang Zainal Hasan Anisa Gianti Zhafira Anna Setiadi Ranti ANNA SETIADI RANTI Aprilita Putri Defan Ritonga Athaya, Ardelia Ayu Tri Nursyarah Aziz Syamsul Huda Aziz Syamsul Huda Aziz Syamsul Huda Azka Adzkya Emalia Putri Bella Fatima Dora Zaelani C Hanny Wijaya Cika Hilda Fransiski Destiandani, Khansa Dewi Purnamasari Dimas Andrianto Dimas Andrianto DIMAS ANDRIANTO Dinar Mutiara Hikmah Djarot Sasongko Hami Seno Dwicesaria, Maheswari Alfira Dwitasari, Oczhinvia Dwitasari, Oczhinvia Eka Wahyu Purnama Etik Mardliyati, Etik Fachriyan H Pasaribu Faoziduhu Bawamenewi FARAH FAHMA Febrina, Adella Fernanda Chairunisa Fernanda Chairunisa Fitria Tika Pradita Fitriana S Monisa Fitrilia, Tiana Gholam, Gusnia Meilin Hanif Alamudin Manshur Hasim Hasim Hasim Hendra Susanto Hudayanti, Martini Husnawati Husnawati Husnawati, . Ilham Gilang Cahya Ahmada Imanniar Yuta Ellana Dittama Indariani, Susi Iriani Setyaningsih Irma Rahmayani Irsal, Riyan Alifbi Putera Kartika, Yayuk Kustiariyah Tarman LAKSMI AMBARSARI Maheswari Alfira Dwicesaria Maheswari Alfira Dwicesaria Mala Nurilmala MARIA BINTANG Maria Bintang Maulana, Farhan Mere, Janrigo Klaumegio Meydia Meydia Mita Gebriella Inthe Monita Rekasih Muhammad Assyar Mustika Weni Mustika Weni Mustika Weni Mustopa, Syahrul Mutmainnah Agustiawan Umar Mutmainnah Umar Nabilla, Rahmah Neni Widowati Nirmala Peni Sugiharti Nur Azizah Nusyarah, Ayu Tri Oczhinvia Dwitasari Paramitha Wirdani Ningsih Marlina Pipih Suptijah Pipih Suptijah Prayoga Pannindriya Prayoga Pannindrya Purwanto, Ukhradiya Magharaniq Safira Puspa Julistia Puspita Putu Kristiani Kalontong Rahadian Pratama Rahmadi Ganesha Putri Raisyadikara, Fadila Rini Kurniasih, Rini Riska Susila Putri Riyan Alifbi Putera Irsal Riyan Alifbi Putera Irsal Rizsa Mustika Pertiwi Rori Theresia Rosalina Yuliani Rosyidah, Rara Annisaur Safira P, Ukhradiya Magharaniq Saputra, Rahadita Maura Amanda Sari, Sintia Permata SEDARNAWATI YASNI Septaningsih, Dewi Anggraini Septiyani, Dinie Shafillah Miantika Silitonga, Ria Gusharani Sri Novita Sagita Sulistiyani , Susanto, Hendra Susi Indariani Susi Indariani Syaefudiin, Syaefudin Syaefudin Syaefudin Syamsul Falah Takeshi Katayama Theresia, Rori Tiara Fibri Yuniasih Tjahja Muhandri Toshisada Suzuki Ukhradiya Magharaniq Safira Purwanto Umar, Mutmainnah Agustiawan Uswatun Hasanah Vitriyanna Mutiara Yuhendri Wong, Chen Wai Yanti Fajarwati Yundari, Yundari Zaelani, Bella Fatima Dora Zatta Yumni Ihdhar Syarafina