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All Journal HAYATI Journal of Biosciences Jurnal Farmasi Udayana JURNAL KIMIA SAINS DAN APLIKASI Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Prosiding Seminar Biologi Jurnal Kesehatan Journal of Tropical Biodiversity and Biotechnology BIOEDUSCIENCE Jurnal Kimia Terapan Indonesia ANNALES BOGORIENSES Jurnal Ilmiah Kesehatan Pharmacoscript Jurnal Riset Biologi dan Aplikasinya Sainstech Farma: Jurnal Ilmu Kefarmasian Jurnal Bina Pengabdian Kepada Masyarakat Jurnal Farmasi Etam Mestaka: Jurnal Pengabdian Kepada Masyarakat GEMBIRA (Pengabdian Kepada Masyarakat) PEDAMAS (Pengabdian Kepada Masyarakat) Science and Technology: Jurnal Pengabdian Masyarakat JCSE: Journal of Community Service and Empowerment
Syafriana, Vilya
Fakultas Farmasi, Institut Sains Dan Teknologi Nasional
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Journal : ANNALES BOGORIENSES

 1 
Enhancement of β-Glucosidase Activity in Penicillium sp. by Random Mutation with Ultraviolet and Ethyl Methyl Sulfonate Vilya Syafriana; Sukma Nuswantara; Wibowo Mangunwardoyo; Puspita Lisdiyanti
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (450.422 KB) | DOI: 10.14203/ann.bogor.2014.v18.n2.27-33

Abstract

The genus Penicillium has a potential ability to produce β-glucosidase. The aim of the study was to improve the β-glucosidase activity of Penicillium sp. ID10-T065 with physical (Ultraviolet = UV), chemical (Ethyl Methyl Sulfonate = EMS), and combined mutation (UV-EMS). The spores of Penicillium sp. ID10-T065 were exposed into UV irradiation for 3 minutes with dose of 0.1 J/cm2 and 13 cm of distances. Chemical mutation was done by treated spores into 3% of EMS solution for an hour. Combined mutation of UV and EMS were also performed by UV for 3 minutes (0.1 J/cm2, 15 cm) and continued with soaking into 2-3% of EMS solution. The developed mutants were screened, selected and assayed. Comparison of enzyme activities with the wild- type (1.78 U/ml), mutant UV13 (5.53 U/ml) showed a 3.1 fold increase; mutant EM31 (4.26 U/ml) showed a 2.4 fold increase. Meanwhile, mutant UM23 obtained from the multiple exposures showed a decreased activity (1.75 U/ml). Mutant UV13 showed the best enzyme activity to be considered as a potential strain for β-glucosidase producer. This result needs to be further elaborated especially on its genetic stability studies in order for the ascertained as a stable mutant.
Co-Authors Ahmad Wildan Al-Mukholladun Ainun Wulandai Ainun Wulandari Akhatik, Nurul Aliya, Lisana Sidqi Ami Rahmawati Sukamto Ana Yulyana Ananda Digita Andini, Issri Mila Nova Arsa, Imalia Aslamiyah Putri Aulia Rahimi Carla Febriayu Ramadhani Chori Azizah Citra Yuditha Fadillah Daini Amanah Dea Selia Dede Heri Yuli Yanto Deni Zulfiana Devi Purwita Sari Dian Puspita Dini Juliana Diny Auliya Mawarni Dwi Okta Mellya Erna Yanti Erwi Putri Setyaningsih Eva Oktavia Fathin Hamida Febriani , Amelia Febriani, Amelia Fitria Helma Fitria Rahayu Fitria Yustisiani Herdini Herdini , Herdini Ika Maruya Kusuma Jaya, Rendy Indra Jessica Khairunnisa Kusuma , Ika Maruya Kusuma, Ika Maruya Lenggo Elfira Lidia Anggita Ramadhani Lili Musnelina Lisana Sidqi Aliya M. Poeloengan Marliyah, Tiah Marsa Falihah Muh. Faris Hidayat Mulandita Munawarohthus Sholikha Munawarohthus Sholikha Munawarohthus Sholikhah Munawarohthus, Sholikha Musnelina , Lili Nafisah Nuha Nanda Sabbaha Nur Kasfillah Nanda, Elsa Vera Nissa, Maftucha Nitya Wita Utama Nuke Paramitha Dewanti Nurfitri Nurfitri Nurul Akhatik Nurul Laili Nurul Natasha Oktari, Anggi Irma Yani Padmono Citroreksoko Pontoan, Jenny Pratiwi, Della Puspita Lisdiyanti PUSPITA LISDIYANTI Putri, Elvina Triana R.D. Rentiana Rabitha Rusyita Rachmatiah, Tiah Rani Damayanti Ratna Nilam Cahya Refdanita Renita Noviani Purba Resti Sofia Zahara Rika, Amelia Risma Eka Putri Ritha Widyapratiwi Rohmawati, Fitri Rosario Trijuliamos Manalu Rosario Trijuliamos Manalu Saiful Bahri Shella Utami Sholikhah , Munawarohthus Siswati Siswati Sita Heris Anita Siti Fadila Azahra Siti Koriah, Siti Subaryanti Subaryanti, Subaryanti Suci Tiara Sukma Nuswantara Sukma Nuswantara SULISTIANI SULISTIANI Suyatno Suyatno Tatik Khusniati Taufiqurrahman, Muh Teodhora, Teodhora Teti Indrawati Tiah Rachmatiah Tiah Rachmatiah Tiah Rachmatiah Tri Soehartati Trisanti Anindyawati Vera Nanda, Elsa Veryanti, Putu Rika Wahidin Wahidin Wibowo Mangunwardoyo WIBOWO MANGUNWARDOYO Widyapratiwi , Ritha Windri, Handayani Wulandari , Ainun Yasman Yasman Yasman Yasman Yayah Siti Djuhariah Yayah Siti Djuhariah Yuliawati, Chyntia Yunisa Simehete Zabitha Zahwa Zahra, Aishah Az