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All Journal Research Journal of Life Science Journal of Tropical Plant Protection AGRIVITA, Journal of Agricultural Science Jurnal Perlindungan Tanaman Indonesia AGROMIX Viabel : Jurnal Ilmiah Ilmu-Ilmu Pertanian Agro Bali: Agricultural Journal Jurnal Agrinika : Jurnal Agroteknologi dan Agribisnis Agroradix : Jurnal Ilmu Pertanian Jurnal HPT (Hama Penyakit Tumbuhan)
Mintarto Martosudiro
Departemen Hama Dan Penyakit Tumbuhan Fakultas Pertanian Universitas Brawijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

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Detection of Onion Yellow Dwarf Virus (OYDV) in Onion Commodities Using DAS-ELISA and RT-PCR at The Point of Entry for Carrier Media at Semayang Port, Balikpapan Zulverdi, Yudhika Tsabitah Ramacendani; Martosudiro, Mintarto
Journal of Tropical Plant Protection Vol. 7 No. 1 (2026)
Publisher : University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jtpp.2026.007.1.1

Abstract

Plants of the genus Allium, such as shallots (Allium cepa var. aggregatum), garlic (Allium sativum L.), green onions (Allium fistulosum L.), and onions (Allium cepa var. cepa), are high-value commodities widely consumed in Indonesia. However, Allium production in East Kalimantan does not meet local demand, necessitating supplies from other regions. This inter-regional transport poses a risk of introducing organisms harmful to plants. Among these pests, viruses are particularly challenging to control. According to the Regulation of the Minister of Agriculture No. 25 of 2020, Onion Yellow Dwarf Virus is classified as a quarantine pest under category A2 requiring strict monitoring in Indonesia. Its distribution is currently limited to Java, Bali, West Nusa Tenggara, and Sulawesi. Therefore, accurate detection techniques are critical to prevent the spread of this pathogen to areas still free from Onion Yellow Dwarf Virus. Research results indicate that among the four types of onions, Onion Yellow Dwarf Virus (OYDV) exhibits symptoms in garlic. This finding was also confirmed through DAS-ELISA and RT-PCR testing. The detection results using DAS-ELISA on garlic plants showed an ELISA Reader absorbance value (0.6848) greater than twice the absorbance value of the negative control (0.2982). RT-PCR detection also yielded positive results with the formation of a 500 bp band.
Co-Authors Abdul Choliq, Fery Abdul Latief Abadi Ajeng Wulandari Ali Pramono Alifa, Desy Hawania Asha Amanda Yayu Natasya Amanda Yayu Natasya Aminudin Afandhi Aminudin Afandhi Anna Sartika Hutapea Anton Muhibbudin Anton Muhibuddin Anugerah Firmansyah Zakaria Anugerah Firmansyah Zakaria Ari Kristini Aritonang, Awal Josua Athifa Lithiflika K. Yahya Aviva Aviolita Parama Putri Aviva Aviolita Parama Putri Aviolita Parama Putri Aviva Aviolita Parama Putri Aviolita Parama Putri Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Bedjo Devi Febriana Putri Devi Febriana Putri Devy Intan Arlita Devy Intan Arlita Dian Eka Kusumawati Effendi, Mokhtar Eka Putri Irma Nuviani Ernawati, Feny Esti Yuliastri Sa’idah Esti Yuliastri Sa’idah Fanhash Nijami, Muhammad Ferry Abdul Choliq Fery Abdul Choliq Fiqh Thamrotul Irsyadah Fiqh Thamrotul Irsyadah Firdausi, Wita Hagus Tarno Haniatur Rochifah Hasanah, Ifa Maulidah Hesty Maranticha I Ketut Suada istiqomah istiqomah Istiqomah, Istiqomah Jayanti Elok Cayah Ambarwati Jayanti Elok Cayah Ambarwati Kamila Qurota A’yun Kamila Qurota A’yun Kestrilia Rega Prilianti Liliek Sulistyowati Lilik Koesmihartono Putra Lilik Nur Kholidah Lilik Nur Kholidah Lilya Echa Febriyanti Luqman Qurata Aini Luqman Qurota Aini M. Guruh Arif Zulfahmi M. Guruh Arif Zulfahmi Mahfud, Muhammad Afif Miko Putro Hutomo Miko Putro Hutomo Muhamad Ihsal Mahendra Nadia Agung Triwibawa Nadia Agung Triwibawa Nofia, Nita Nurwijayanti Prabowo, Matheus Randy Qurrota Ayuni Apriliana Rahayu, Esti Dwi Rasyada, Nuris Sa’adah Rois Hanadyo Rois Hanadyo Roswita Nur Kumalasari Rosyida, Risya Safira Candra Jalaweni Safitri, Helvira Agita Saidi, Aluna Uthilma Salsabilla, Alyara Oktavia Samsul Huda Asrori Sinta Asa Karsalina Sofyaningrum, Alaida Nur Laili Sunarto, Bintar Probo Syamsuddin Djauhari Tashya Angelique Martinez Tutung Hadi Astono Tutung Hadiastono Tutung Hadiastono Tutung Hadiastono Tutung Hadiastono Ulil Azmi Ulil Azmi Uswatun Hasanah Uswatun Hasanah Vivi Tri Kristyaningrum Yayang Cahyaning Bulan Yayang Cahyaning Bulan Yayuk Yuliati Yugista, Serdinia Zulverdi, Yudhika Ankenkuala Ramadhana Zulverdi, Yudhika Tsabitah Ramacendani