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Pengujian Aktivitas Antibakteri dan Antioksidan serta Kadar Fenol Total Ekstrak Buah dan Kulit Buah Apel Hijau (Malus sylvestris (L.) Mill) Amelia Ersa Renatha; Citra Mufidah; Vinda Maharani Patricia; Yani Lukmayani; Kiki Mulkiya
Bandung Conference Series: Pharmacy Vol. 4 No. 2 (2024): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsp.v4i2.15064

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Abstract. Tropical fruits have the potential to be a natural source of antioxidants. Natural antioxidants are generally considered safer for consumption and can improve overall health compared to synthetic antioxidants. One plant that exhibits promising antioxidant properties is the green apple (Malus sylvestris (L.) Mill), which is rich in vitamin A, vitamin B, vitamin C, minerals, fiber, and phenolic compounds. This study aimed to evaluate the antibacterial activity against Propionibacterium acnes and Pseudomonas aeruginosa, antioxidant activity using the DPPH (2,2-diphenyl-1- picrylhydrazyl) method, and total phenolic content of both the fruit and peel of green apples that were ground in water at a ratio of (1:10). The results showed that the fruit juice and peel extracts of green apples exhibited a moderate zone of inhibition against Propionibacterium acnes and Pseudomonas aeruginosa, falling within the 5-10 mm range. However, the peel juice extract demonstrated a larger zone of inhibition against both bacteria compared to the fruit juice extract. The antioxidant activity of both the fruit and peel extracts was categorized as weak. The green apple fruit extract exhibited an IC50 value of 597.4853 ppm and a total phenolic content of 8.4762 mgGAE/g, while the peel extract demonstrated an IC50 value of 583.0589 ppm and a total phenolic content of 13.315 mgGAE/g. Abstrak. Buah-buahan tropis berpotensi sebagai sumber antioksidan alami. Antioksidan alami umumnya lebih aman untuk dikonsumsi dan dapat meningkatkan derajat kesehatan tubuh jika dibandingkan dengan antioksidan sintetik. Salah satu tanaman yang berpotensi memiliki khasiat sebagai antioksidan adalah tanaman apel hijau (Malus sylvestris (L.) Mill) yang banyak mengandung vitamin A, vitamin B, vitamin C, mineral, serat serta senyawa fenol. Penelitian ini bertujuan untuk menguji aktivitas antibakteri terhadap Propionibacterium acnes dan Pseudomonas aeruginosa, pengujian aktivtias antioksidan terhadap DPPH (2,2-diphenyl-1- picrylhydrazyl) serta menghitung kadar fenol total dari buah dan kulit buah apel hijau yang dihaluskan menggunakan air dengan perbandingan (1:10). Hasil penelitian menunjukan bahwa ekstrak jus buah dan kulit buah apel hijau terhadap bakteri Propionibacterium acnes dan Pseudomonas aeruginosa memiliki respon zona hambat sedang karena berada di rentang nilai 5-10 mm, sedangkan hasil dari ekstrak jus kulit buah apel memiliki diameter zona hambat lebih besar terhadap bakteri Propionibacterium acnes dan Pseudomonas aeruginosa dibandingkan dengan ekstrak jus buah apel hijau. Pengujian aktivitas antioksidan ekstrak buah dan kulit buah apel hijau masing-masing termasuk ke dalam kategori lemah. Ekstrak buah apel hijau memberikan nilai IC50 sebesar 597,4853 ppm dengan nilai kadar fenol total sebesar 8,4762 mgGAE/g, sedangkan untuk ekstrak kulit buah apel hijau memberikan nilai IC50 sebesar 583,0589 ppm dengan nilai kadar fenol total sebesar 13,315 mgGAE/g.
Karakterisasi Simplisia dan Ekstrak Daun Tembakau (Nicotiana tabacum L.) Varietas Besuki Herna Haryani; Farendina Suarantika; Vinda Maharani Patricia
Bandung Conference Series: Pharmacy Vol. 4 No. 2 (2024): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsp.v4i2.15098

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Abstract. Tobacco (Nicotiana tabacum) is an annual herb in the Solanaceae family. In Indonesia, tobacco is a very well-known type of plant. Apart from containing essential oils, tobacco leaves contain many bioactive compounds. Where plant bioactive compounds are very important for human health because of their various biological effects, such as antioxidant, anticarcinogenic, antiallergic, anti-inflammatory, antimutagenic and antimicrobial activities, which can provide beneficial effects on various non-communicable diseases, such as autoimmune, inflammatory, cardiovascular, cancer, disease. metabolic, and neurodegenerative. The aim of this research is to determine the characteristics of the Besuki variety tobacco leaf simplicia. The results obtained were that tobacco leaf simplicia had a drying shrinkage value of 9.65%; water content 8.59%; total ash content 19.17%, acid insoluble ash content 0.78%; water soluble essence content 34.64%; ethanol soluble essence content 15.62%. The results of the characterization of the extract obtained a percent yield value of 2.3% and a specific gravity of 0.692 g/mL. These results can be concluded that simplicia and tobacco leaf extract meet the standard quality parameters and SNI requirements. Abstrak. Tembakau (Nicotiana tabacum) merupakan salah satu tanaman kontroversial dalam keluarga Solanaceae Di Indonesia Tembakau merupakan salah satu jenis tanaman yang sangat terkenal. Selain banyak mengandung nikotin, tembakau juga mengandung minyak atsiri, daun tembakau ini mengandung banyak senyawa bioaktif. Dimana senyawa bioaktif tumbuhan sangat penting bagi kesehatan manusia karena berbagai efek biologisnya, seperti aktivitas antioksidan, antikarsinogenik, antialergi, antiinflamasi, antimutagenik, dan antimikroba, yang dapat memberikan efek menguntungkan pada berbagai penyakit tidak menular, seperti autoimun, inflamasi, kardiovaskular, kanker, penyakit metabolik, dan neurodegeneratif. Tujuan dari penelitian ini adalah untuk mengetahui karakteristik dari simplisia daun tembakau varietas Besuki. Metode yang digunakan adalah penelitian eksperimental di Laboratorium riset Farmasi Unisba. Hasil yang didapatkan bahwa simplisia daun tembakau memiliki nilai susut pengeringan sebesar 9,65%; kadar air 8,59%; kadar abu total 19,17%, kadar abu tidak larut asam 0,78%; kadar sari larut air 34,64%; kadar sari larut etanol 15,62%. Hasil karakterissi pada eksrat didapatkan nilai persen rendemen sebesar 2,3% dan bobot jenis sebesar 0,692 g/mL. Hasil tersebut dapat disimpulkan bahwa simplisia dan ekstrak daun tembakau memenuhi syarat standar parameter mutu dan SNI.
Uji Sitotoksik Kulit Buah Naga Merah (Selenicereus monacanthus (Lem.) D.R.Hunt) dengan Metode Brine Shrimp Lethality Test (BSLT). Gishela Bellania; Kiki Mulkiya Yuliawati; Vinda Maharani Patricia
Bandung Conference Series: Pharmacy Vol. 4 No. 2 (2024): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsp.v4i2.15221

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Abstract. Red dragon fruit peel is now widely sold as a health medicine consumed by consumers. Therefore, it is necessary to carry out tests to determine the effectiveness and safety of the product. This research aims to determine the ethanol extract produced from the maceration and digestion methods at concentrations (62.5 ppm, 125 ppm, 250 ppm, 500 ppm, 1000 ppm) for 24 hours. The method used was the Brine Shrimp Lethality Test (BSLT) which is measured in the death of Artemia fransiscana. The results of the research showed that the LC50 value of red dragon fruit peel maceration extract and digestion were 75.25 μg/ml and 96.09 μg/ml. respectively and the LC50 value for digestion was 96.09 μg/ml. With an LC50 value of more than 30 ppm, the ethanol extract of red dragon fruit peel produced by maceration or digestion methods is toxic. Abstrak. Kulit buah naga merah kini banyak dijual sebagai obat kesehatan yang dikonsumsi oleh para konsumen. Oleh karena itu, perlu dilakukan pengujian untuk mengetahui efektivitas dan keamanan produk. Penelitian ini bertujuan untuk mengetahui ekstrak etanol yang dihasilkan dari metode maserasi dan digesti pada konsentrasi (62,5 ppm, 125 ppm, 250 ppm, 500 ppm, 1000 ppm) selama 24 jam. Metode yang dilakukan adalah Brine Shrimp Lethality Test (BSLT) yang diukur terhadap kematian Artemia fransiscana. Hasil penelitian menunjukkan bahwa nilai LC50 ekstrak kulit buah naga merah ekstrak maserasi dan digesti berturut-turut sebesar 75,25 μg/ml dan 96,09 μg/ml. Dengan nilai LC50 lebih dari 30 ppm, maka ekstrak etanol kulit buah naga merah yang dihasilkan dengan metode maserasi ataupun digesti bersifat toksik.
Pengujian Kadar Antosianin Bunga Pagoda (Clerodendrum paniculatum L.) Berdasarkan Variasi Penambahan Asam Meytha Putri Dewanty; Livia Syafnir; Vinda Maharani Patricia
Bandung Conference Series: Pharmacy Vol. 4 No. 2 (2024): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsp.v4i2.15515

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Abstract. Anthocyanin is a natural coloring that belongs to the flavonoid group. Pagoda flowers have anthocyanins which can be separated by choosing an extraction method to find out the right extraction to induce the highest levels of anthocyanins in pagoda flowers because anthocyanins are unstable compounds. This research aims to determine the level of pagoda flowers. Anthocyanin levels were determined using a differential pH strategy with Uv-Vis spectrophotometry. The results of determining the concentration of anthocyanin levels in pagoda flowers in 1% HCl were 8,75 mg/100 g and in potassium acetate they were 1,20 mg/100g. Abstrak. Antosianin merupakan warna alami yang termasuk dalam golongan flavonoid. Bunga pagoda mempunyai antosianin yang dapat dipisahkan dengan memilih metode ekstraksi untuk mengetahui ekstraksi yang tepat untuk menginduksi kadar antosianin tertinggi pada bunga pagoda karena antosianin merupakan senyawa tidak stabil. Penelitian ini bertujuan untuk menentukan kadar pada bunga pagoda . Kadar antosianin ditentukan menggunakan strategi pH diferensial dengan spektrofotometri Uv-Vis. Hasil penetapan konsenterasi kadar antosianin bunga pagoda pada HCl 1% adalah sebesar 8,75 mg/100 g dan pada kalium asetat adalah sebesar 1,20 mg/ 100g.
Karakterisasi Larutan Eco-Enzyme dari Kulit Buah Nanas (Ananas comosus L.) Merr. dan Kulit Buah Naga (Hylocereus polyrhizus) (F.A.C. Weber) Britton & Rose Zaina Alviona Pridesta; Dina Mulyanti; Vinda Maharani Patricia
Bandung Conference Series: Pharmacy Vol. 4 No. 2 (2024): Bandung Conference Series: Pharmacy
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsp.v4i2.15559

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Abstract. Pineapple and dragon fruit plants are often consumed for their fruit, leaving behind their peels as organic waste. This organic waste accumulates and negatively impacts the environment. To reduce the accumulation of fruit peels, there is a need to utilize plant peels as raw materials for eco-enzyme solutions. Eco-enzyme is an environmentally friendly enzyme with numerous benefits, produced as a solution by adding sugar and water. This study aimed to determine the organoleptic characteristics and pH of eco-enzyme solutions made from pineapple and dragon fruit peels. The organoleptic characteristics of the eco-enzyme solutions were observed in terms of odor, color, and the formation of a white layer (mother enzyme), while pH was measured using a pH meter. Results showed that after 3 months of fermentation, the eco-enzyme solutions from both pineapple and dragon fruit peels underwent color changes, had a strong acidic odor characteristic of the respective fruits, and exhibited varying amounts of white layer (mother enzyme). The pH of both solutions ranged from 3. The acidic pH obtained from the eco-enzyme solutions of pineapple and dragon fruit peels is promising for potential applications in pharmaceuticals and household products. Abstrak. Tanaman buah nanas dan buah naga seringkali dikonsumsi buahnya sehingga menyisakan kulit buah, yang menjadi sampah organik dan menimbulkan penumpukan yang tidak baik bagi kesehatan lingkungan. Untuk mengurangi penumpukan sisa kulit buah tersebut maka perlu adanya pemanfaatan kulit buah tumbuhan yang dapat dijadikan sebagai bahan baku larutan eco-enzyme. Eco-enzyme adalah enzim ramah lingkungan yang memiliki banyak manfaat dan dibuat dalam bentuk larutan dengan penambahan gula dan air. Tujuan penelitian ini adalah untuk mengetahui karakteristik organoleptis dan pH pada kulit buah nanas dan kulit buah naga yang dijadikan larutan eco-enzyme. Metode yang digunakan untuk karakteristik larutan eco-enzyme ini yaitu diamati dari segi bau, warna, dan lapisan putih (mama enzim) yang terbentuk untuk pengamatan organoleptis dan untuk pengujian pH menggunakan alat pH meter. Hasil penelitian diperoleh bahwa karakteristik larutan eco-enzyme kulit buah nanas dan kulit buah naga setelah 3 bulan mengalami perubahan warna seiring lama nya waktu fermentasi, memiliki bau asam menyengat dari buahnya, memiliki lapisan putih (mama enzim) yang berbeda beda dari tiap jenis larutan, serta memiliki pH berkisar 3, pH asam yang didapatkan dari larutan eco-enzyme kulit buah nanas dan kulit buah naga ini diharapkan dapat dimanfaatkan menjadi sediaan farmasi dan perbekalan rumah tangga.
VALIDASI METODE ANALISIS SENYAWA METABOLIT SEKUNDER PADA MORINGA OLEIFERA LAM., DAN EUCALYPTUS GLOBULUS LABILL. Suarantika, Farendina; Akbar, Nabila Hadiah; Patricia, Vinda Maharani
Jurnal Inkofar Vol 8, No 1 (2024)
Publisher : Politeknik META Industri Cikarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46846/jurnalinkofar.v8i1.330

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Beberapa tanaman Indonesia seperti Moringa oleifera Lam. dan Eucalyptus globulus Labill. mengandung senyawa aktif yang dapat memberikan aktifitas farmakologis seperti antibakteri, antioksidan, antidiabetes, anti-kanker dan juga anti-inflamasi. Penelitian ini merupakan studi pustaka yang meninjau validasi metode dalam analisis senyawa aktif yang terdapat pada tanaman Moringa oleifera Lam. dan Eucalyptus globulus Labill. Adapun parameter yang terdapat pada dalam suatu validasi metode yaitu akurasi, linearitas, presisi, batas nilai deteksi, dan batas nilai akurasi. Sumber informasi yang digunakan pada kajian pustaka ini diperoleh dengan menggunakan search engine seperti Science direct, Google Scholar, dan PubMed. Kriteria inklusi mencakup artikel penelitian, tema artikel mengenai analisis metabolit sekunder Moringa oleifera Lam. dan Eucalyptus globulus Labill. Untuk kriteria eksklusi adalah artikel metode analisis metabolit sekunder selain Moringa oleifera Lam. dan Eucalyptus globulus Labill., tidak memuat data validasi, serta tidak memuat prosedur lengkap analisis. Berhasil dikumpulkan dan dianalisis sebanyak 45 artikel dan hasilnya validasi metode secara umum menggunakan instrumen RP-HPLC/HPTLC, Spektrofotometri UV-Vis, GC-FID, GC, dan UHPLCDAD. Kata Kunci: Validasi metode, Moringa oleifera Lam., Eucalyptus globulus Labill., Metabolit sekunder
Aktivitas Antibakteri Ekstrak Etanol Buah Alkesa terhadap S. aureus dan E. coli Fakhrul Akbar Arrahim; Vinda Maharani Patricia; Livia Syafnir
Jurnal Riset Farmasi Volume 4, No. 1, Juli 2024, Jurnal Riset Farmasi (JRF)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jrf.v4i1.3891

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Abstract. Alkesa fruit (Pouteria campechiana (Kunth) Baehni) has many benefits, one of which is suspected to possess pharmacological activity as an antibacterial agent. Staphylococcus aureus and Escherichia coli are pathogenic bacteria that commonly cause diseases in humans, such as diarrhea. The aims of this study was to determine the antibacterial activity of the ethanol extract of alkesa fruit against Staphylococcus aureus and Escherichia coli, as well as to determine the Minimum Inhibitory Concentration (MIC). The antibacterial activity test was conducted using the agar diffusion method with ethanol extract of alkesa fruit at concentrations ranging from 35% to 100% (w/v). The results showed that the ethanol extract of alkesa fruit exhibited the same level of activity against both bacteria, with the same minimum inhibitory concentration, which is 36%. Abstrak. Buah alkesa (Pouteria campechiana (Kunth) Baehni) memiliki banyak manfaat yang salah satunya diduga memiliki aktivitas farmakologi sebagai antibakteri. Bakteri Staphylococcus aureus dan Escherichia coli merupakan bakteri patogen yang biasanya menimbulkan penyakit pada manusia contohnya yaitu penyakit diare. Tujuan penelitian ini untuk mengetahui aktivitas antibakteri ekstrak etanol buah alkesa terhadap Staphylococcus aureus dan Escherichia coli serta menentukan Konsentrasi Hambat Minimum. Pengujian aktivitas antibakteri dilakukan dengan metode difusi agar sumuran menggunakan ekstrak etanol buah alkesa dengan rentang konsentrasi 35% - 100% (b/v). Hasil pengujian menunjukkan bahwa ekstrak etanol buah alkesa memiliki aktivitas  yang sama terhadap kedua bakteri dengan konsentrasi hambat minimum yang sama yaitu 36%.
Formulation and Physical Characterization of Onion (Allium ascalonicum L) Bulb Extract in Cream Using Tween 80 and Span 60 as the Emulgator Fauzia Ningrum Syaputri; Vinda Maharani Patricia
Jurnal Sains dan Kesehatan Vol. 2 No. 2 (2019): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v2i2.138

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In Indonesia, it is not uncommon to find children who suffer from fever which can occur due to the change of weather from the rainy season during the dry season or vice versa. Communities in rural areas generally use onion (Allium ascalonicum L) to relieve fever in children. Cream preparations are made by varying the concentration of the emulgator to determine the effect of different concentrations of the emulgator on the physical characteristics of the cream preparation. The emulgator used is tween 80 and span 60 with a concentration of Formula 1: 3.5%, Formula 2: 3.75%, formula 3: 4%. Physical evaluations carried out in this study were organoleptic, pH, homogeneity, cream washability, cream type, viscosity, spreadability, adhesion, and freeze-thaw tests. The results of the study it can be concluded that the differences in the concentration of the emulgator can affect the physical characteristics of the cream preparation, namely in the spreading test and the adhesion test shows that F3 (4%) can reduce the spreadability of the cream preparation but can be attached to a long time when compared to F1 (3.5%) and F2 (3.75%). The physically optimal cream preparation formula is F1 and F2 because it meets all the requirements in the evaluation test. Keywords: Cream, onion, emulgator, physical characteristics
Material Flow and Economic Analysis of Wantilan Recycling Center Promoting Circular Economy Principles Satori, Mohamad; Hindersah, Hilwati; Burhanudin, Hani; Januarita, Ratna; Purnamasari, Pupung; Hernawati, Riza; Patricia, Vinda Maharani; Miftahadi, Machmuddin Fitra
Jurnal Presipitasi : Media Komunikasi dan Pengembangan Teknik Lingkungan Vol 21, No 3 (2024): November 2024
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/presipitasi.v21i3.722-733

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Waste is increasingly recognized not just as an environmental challenge but also as an economic opportunity, particularly in developing countries like Indonesia where it can help alleviate employment shortages. This study explores the dual role of waste as both a tradable commodity driven by industrial demands for raw materials, including primary substances, additives, and Refuse Derived Fuel (RDF), and as a means of reducing environmental impact. This phenomenon is important and interesting because it can have a positive impact both in terms of reducing waste, saving natural resources, and as an economic opportunity for society. This study aims to examine the potential of waste that can be utilized as a resource on the one hand and reduce the potential for pollution on the other. The study used the Material Flow Analysis (MFA) method with the Circular Economy principle and used an action research approach to redesign the waste processing system. The results of this study indicate that the current system only manages 34% of the total waste generation and only 4% can be reused with sales of IDR 97.9 million per year. With the development of a new system, the waste managed is 60% with the economic potential generated of 1.08 billion per year.
Produksi Hidrolisat Protein Maggot Black Soldier Fly (Hermetia illucens) dengan Menggunakan Enzim Tripsin Cahyaning Pertiwi; Vinda Maharani Patricia; Bertha Rusdi
Jurnal Riset Farmasi Volume 5, No. 1, Juli 2025, Jurnal Riset Farmasi (JRF)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jrf.v5i1.6812

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Abstract. Protein hydrolysate is a protein breakdown product in the form of small peptide fragments obtained through enzymatic hydrolysis. This study aims to determine the yield of protein hydrolysate from Black Soldier Fly (BSF) maggot using trypsin enzyme. The results indicate that enzymatic hydrolysis of BSF maggot protein with trypsin produced an average yield of 37.33 mg/g. The use of trypsin did not exhibit a statistically significant difference compared to the control group, with a P-value of 0.130, which exceeds the significance threshold (α = 0.05). Abstrak. Hidrolisat protein adalah produk pemecahan protein berupa fragmen kecil peptida yang diperoleh melalui reaksi hidrolisis enzimatik. Penelitian ini bertujuan untuk memperoleh rendemen hidrolisat protein dari maggot BSF (Black Soldier Fly) dengan menggunakan enzim tripsin. Hasil penelitian menunjukkan bahwa hidrolisis enzimatik protein maggot BSF dengan menggunakan enzim tripsin mampu menghasilkan rendemen dengan rata-rata bobot sebesar 37,33 mg/g. Penggunaan enzim tripsin tidak menunjukkan perbedaan bermakna dibanding kelompok kontrol dengan P-value sebesar 0.130 yang lebih besar dari tingkat signifikansi (α = 0,05).