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All Journal Jurnal Kesehatan Lingkungan indonesia Biosfer: Jurnal Tadris Biologi Cannarium (Jurnal Ilmu-ilmu Pertanian) Science and Technology Indonesia Jurnal Biologi Tropis Jurnal Vokasi Prima Abdika: Jurnal Pengabdian Masyarakat Sriwijaya Bioscientia Indonesian Journal of Applied Environmental Studies BIOVALENTIA: Biological Research Journal Molekul: Jurnal Ilmiah Kimia Majority Science Journal BIO PALEMBANICA CONSERVA : Jurnal Konservasi Sumber Daya Alam dan Lingkungan
Arum Setiawan
Jurusan Biologi FMIPA, Universitas Sriwijaya
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Journal : Jurnal Biologi Tropis

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Primer Design of Sumatran Striped Rabbit (Nesolagus netscheri Schlegel, 1880) using Primer-BLAST and AliView Program Aurora, Dhea Apriano; Novarino, Wilson; Tjong, Djong Hon; Dahelmi, Dahelmi; Syaifullah, Syaifullah; Setiawan, Arum; Roesma, Dewi Imelda
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8499

Abstract

The Sumatran striped rabbit (Nesolagus netscheri) lacks specific primers to amplify the chytochrome oxidase 1 (CO1) gene and the chytochrome b (cytb) gene, at present. Therefore, it is important to design primers to amplify the CO1 gene and cytb gene in N. netscheri. The aim of this study is to compare the primer design methods used, namely Primer-BLAST and AliView programs, to design specific primers for the chytochrome oxidase 1 (CO1) and chytochrome b (cytb) genes in N. netscheri. This research was conducted using the descriptive method with molecular observation. In this study, CO1 gene primers, namely [(forward: 5' TGTATGATATGGGGGAGGGC 3'), (reverse: 5' TGGTCCGTCCTTATTACAGCG 3')] and cytochrome b (cytb) gene primers, namely [(forward: CCAGCTCCATCCAATATCTC, (reverse: 5' GTTAGGGTTAGAAGGTCTGC 3')] and showed that primer design using the AliView program produced specific primers in the genus Nesolagus. The conclusion of this study is that primers designed using the AliView program are more specific than those designed using Primer-BLAST.
Co-Authors Aetin, Entin Nur Affandi, Azhar Kholiq Aini, Intan Nurul Alexander Alexander, Alexander Anita Desiani Aprilia, Ina Aprillia, Ina Aprillia, Ina Arfan Abrar Aurora, Dhea Apriano Cahyaningrum, Putri Chandra Irsan Dahelmi Dahelmi Denny Noberio, Denny Dewi Imelda Roesma Djong Hon Tjong Doni Setiawan Ekowati, Indah Agustina Elfita Elfita Elfita Elvi Sunarsih Enggar Patriono, Enggar Harmoko Harmoko, Harmoko Hary Widjajanti Indra Yustian Indrianti, Winda Indriati, Winda Irmeilyana Istriati Istriati, Istriati Istriyati Istriyati Kadarisman, Reza Kurniawati, Alfia Rahma Kusuma, Shinta Kusuma, Shinta Laila Hanum Maharadatunkamsi Mammed Sagi Mardiyanto Melto Sevli, Melto Muhammad Gunawan Muhammad Iqbal Muhammad Iqbal Muharni Muharni Nadya Rahmania, Nadya Ngudiantoro . Novitrie, Ayu Oktaberi, Redi Oktiansyah, Rian Oktora Dwi Putranti Pormansyah Pragustiandi, Guntur Pratiwi, Putri Rizki Prima, Bayu Rio Firman Saputra, Rio Firman Risma Handayani, Risma Ryan Hidayat, Ryan Safri, Adios Salni Samitra, Dian Sepriyaningsih, Sepriyaningsih Sri Indra Maiyanti Sulistyadi, Eko Syaifullah Syaifullah Syarifah Syarifah, Syarifah Wenny Saptalisa, Wenny Widjajanty, Hary Widya Asmara Widya Ismara, Widya Wilson Novarino Wiwik Handayani Yuanita Windusari Yundari, Yundari Zubaidah R, Zubaidah Zulkifli Dahlan