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Bioassay of phosphorus solubilizing isolates for enhance P solubility and growth of rice (Oryza sativa L.) Fitriatin, Betty Natalie; Manurung, Dahlia Florencia; Setiawati, Mieke Rochimi
Jurnal AGRO Vol 9, No 1 (2022)
Publisher : Jurusan Agroteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/17754

Abstract

Fosfat merupakan salah satu unsur yang berperan penting bagi pertumbuhan tanaman dan kesuburan tanah. Namun, ketersediaan unsur P terlarut yang dapat diserap oleh tanaman sangat kecil karena berikatan dengan kation yang berada di dalam tanah. Salah satu upaya dalam meningkatkan P tersedia dalam tanah adalah dengan pemanfaatan agen hayati Bakteri Pelarut Fosfat (BPF). Penelitian ini bertujuan untuk menguji isolat BPF yang dapat meningkatkan kelarutan P dan pertumbuhan padi pada uji hayati. Percobaan ini dilaksanakan di Rumah Kaca Departemen Ilmu Tanah dan Sumber Daya Lahan, Fakultas Pertanian, Universitas Padjadjaran. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok (RAK) dengan 6 perlakuan dan 5 ulangan. Masing-masing perlakuan jenis bakteri adalah kontrol, Bacillus substilis, B. megatherium, Pseudomonas mallei, Burkholderia sp., dan isolat campuran. Hasil percobaan menunjukkan isolat BPF yang diuji memiliki kemampuan yang bervariasi dalam meningkatkan enzim fosfatase, kelarutan P, dan pertumbuhan padi pada uji hayati. Lebih lanjut, perlakuan BPF campuran memberikan pengaruh lebih baik terhadap aktivitas fosfatase, P terlarut dan pertumbuhan padi dibandingkan isolat tunggal.ABSTRACTPhosphorus is an element that important for soil fertility and plant growth. However, the phosphate nutrient can be uptaken by plants only in a small amount because it binds to cations in the soil. The effort for enhancing the soil P availabilty is by the phosphorus solubilizing bacteria (PSB). This study aimed to test PSB  isolates  for increasing P solubility and rice growth using bioassay. The experiment conducted at the greenhouse in Jatinangor District, Sumedang Regency, West Java with  Randomized Block Design (RBD) for PSB isolates with five replications. Each type of bacteria treatment was control, Bacillus substilis, B. megatherium, Pseudomonas mallei, Burkholderia sp., and mixed isolates. The results showed that the P solubilizing  isolates had various abilities to enhance phosphatase, P solubility, and rice growth using bioassay. Furthermore, the mixed PSB isolates had a better effect on phosphatase activity, dissolved P and rice growth than single isolates.
Growth response of tomatoes to application of bacterial-coated NPK fertilizer in a pot experiment Hindersah, Reginawanti; Kaffah, Ruhnayati; Aisyah, Ayu Siti; Setiawati, Mieke Rochimi; Arifin, Mahfud
Kultivasi Vol 23, No 2 (2024): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v23i2.54150

Abstract

Nitrogen-fixer and phosphate-solubilizer bacteria increase the chemical-fertilizer efficiency and soil health. Bacterial-coated NPK fertilizer is a novel approach for intensifying biofertilizer application in vegetable production. The study aimed to observe the compatibility between two Azotobacter species and two Bacillus species, their population on two formulations of bacterial-coated NPK fertilizer (BCN), and the effect of BCN dose on growth, N and P content, and their uptake in tomato shoots. The compatibility test was performed using the streak method. The population of Azotobacter and Bacillus were counted for two formulations of NPK fertilizer coated by solid biofertilizer (5% and 10%) and zeolite (1% and 5%). The pot experiment was conducted in a randomized block design with four treatments and six replications. The treatments were 100% recommended doses of conventional NPK fertilizer (700 kg/ha) and 100%, 80%, and 60% doses of BCN. The results showed four bacterial species were compatible, indicated by synergistic growth on the plate agar. The BCN formula using 5% liquid inoculant and 5% zeolite has higher cell viability. The BCN enhanced stem thickness and leaves number but did not change the plant height, dry weight, N and P content, and their uptake in shoots. Applying 60% of BCN caused greater stem thickness and leaf number. Despite being insignificantly different from another treatment, that dose increased the biomass and the shoot uptake of N and P. The NPK fertilizer coated by Azotobacter and Bacillus has the potency to increase tomato growth and NPK fertilizer dose.
Efek Aplikasi Pupuk NPK cair terhadap P-tersedia, Serapan P, dan Bobot Umbi Bawang Merah (Allium ascalonicum L.) di Tanah Inceptisol Jatinangor Setiawati, Mieke Rochimi; Maharani, Nadhira Saniya; Suryatmana, Pujawati; Hindersah, Reginawanti; Fitriatin, Betty Natalie; Chotimah, Andina; Aditya, Fasa
Soilrens Vol 22, No 2 (2024)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v22i2.61717

Abstract

The increase in corn plant growth is greatly influenced by cultivation techniques includingfertilization. However, the use of inorganic fertilizers for a long period of time can reduce soil quality.An alternative environmentally friendly technology that can substitute for the use of inorganicfertilizers is the biological fertilizer of nitrogen-fixing endotypic bacteria. These endophytic bacteriahave benefits as growth stimulants for plants in increasing nitrogen supply through N2 fixationthrough the air. This study aims to increase the growth of corn plants through the application methodand type of nitrogen-fixing endophytic bacteria. This study used a Randomized Block Design (RBD).The treatment of types and application methods consisted of treatments without endophytic bacterialisolates, endophytic bacterial isolates Gluconacetobacter diazotrophicus Corrig, G. diazotrophicusstrain 4L, and Burkholderia gladioli with three application methods, namely seed treatment, soiltreatment, and their combinations. The results of the study showed that G. diazotrophicus 4L bacteriaapplied by seed and soil treatment gave the best effect on the growth of corn plants on the parametersof the endophytic bacterial population in leaf tissue, wet weight and dry weight of corn by 1 and 3times compared to the control and there was also a tendency to increase the dry and wet weight of thecorn plant crown
Prospects of Azotobacter as a Biofertilizer in Saline Soils Hindersah, Reginawanti; Fitriatin, Betty N; Setiawati, Mieke R; Adawiyah, Aliya Z
JURNAL BUDIDAYA PERTANIAN Vol 21 No 1 (2025): Jurnal Budidaya Pertanian
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/jbdp.2025.21.1.1

Abstract

Climate change causes high waves on the seas that can accelerate the salinization of agricultural land located in coastal areas. Saline soil does not support plant growth because of the limitations of plant roots in absorbing water and nutrients, especially Nitrogen. Increasing salinization causes challenges in finding proper soil management methods, including nature-based solutions. A potential method to strengthen plant growth in saline areas is the introduction of biofertilizers containing nitrogen-fixing bacteria (NFB) that are resistant to salinity. This article aims to review the tolerance of nonsymbolic NFB Azotobacter to salinity and discuss the potential of this beneficial rhizobacterium for mitigating plant cultivation in saline land. The article was compiled through a literature study analyzing various relevant references from national and international journals in the last ten years. Analysis of the references shows that several soil Azotobacter strains are resistant to certain salt levels and have specific physiological characteristics when living in saline environments. Azotobacter induces plant tolerance to high salinity in the pot experiment. Therefore, Azotobacter inoculation becomes one of the alternatives to increase the growth of crops in saline soil.
Respon Tanaman Jagung terhadap Cara Aplikasi dan Jenis Bakteri Endofitik Penambat Nitrogen Setiawati, Mieke Rochimi; Maharani, Nadhira Saniya; Suryatmana, Pujawati; Hindersah, Reginawanti; Fitriatin, Betty Natalie; Chotimah, Andina; Aditya, Fasa
Soilrens Vol 22, No 2 (2024)
Publisher : Dept Ilmu Tanah & Sumberdaya Lahan Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/soilrens.v22i2.64415

Abstract

The increase in corn plant growth is greatly influenced by cultivation techniques includingfertilization. However, the use of inorganic fertilizers for a long period of time can reduce soil quality.An alternative environmentally friendly technology that can substitute for the use of inorganicfertilizers is the biological fertilizer of nitrogen-fixing endotypic bacteria. These endophytic bacteriahave benefits as growth stimulants for plants in increasing nitrogen supply through N2 fixationthrough the air. This study aims to increase the growth of corn plants through the application methodand type of nitrogen-fixing endophytic bacteria. This study used a Randomized Block Design (RBD).The treatment of types and application methods consisted of treatments without endophytic bacterialisolates, endophytic bacterial isolates Gluconacetobacter diazotrophicus Corrig, G. diazotrophicusstrain 4L, and Burkholderia gladioli with three application methods, namely seed treatment, soiltreatment, and their combinations. The results of the study showed that G. diazotrophicus 4L bacteriaapplied by seed and soil treatment gave the best effect on the growth of corn plants on the parametersof the endophytic bacterial population in leaf tissue, wet weight and dry weight of corn by 1 and 3times compared to the control and there was also a tendency to increase the dry and wet weight of thecorn plant crown.
Enhancing Tomato (Lycopersicon esculentum Mill.) Growth in a Green House Using NPK Fertilizer Coated with Endospore-Forming Bacillus Hindersah, Reginawanti; Fitriatin, Betty Natalie; Setiawati, Mieke Rochimi; Suryatmana, Pujawati; Risanti, Rara Rahmatika; Dewi, Yeni Wispa
Agrikultura Vol 36, No 1 (2025): April, 2025
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v36i1.62354

Abstract

Long-term use of NPK composite fertilizers mixed with biofertilizers, including spore-forming Bacillus rhizobacteria, can improve plant quality and growth. This study aims to analyze Bacillus’s growth and spore production in chemical-liquid growing media and to observe the effects of Bacillus-coated NPK formulas (BCN) on the growth of tomatoes in potted Andisol and the Bacillus population in the rhizosphere. The evaluation of Bacillus growth was conducted in the laboratory whereas the effect of the BCN was conducted as a pot experiment in a Randomized Block Design. The treatments included recommended dosage of NPK 16-16-16, liquid inoculant of Bacillus consortium, BCN-C, and BCN-G formulas with 100%, 75%, and 50% of the recommended dosage, respectively. Control plants were not treated with fertilizer. Laboratory assay verified that Yuniarti’s medium supported Bacillus-consortium growth and produced the highest Bacillus vegetative cells and spores. The BCN application significantly increased plant height, number of leaves, root length, and tomato biomass compared to the control up to 6 weeks after planting. Three-quarters of the dosage of BCN-C and BCN-G gave the best growth of tomatoes. Introducing BCN and conventional NPK did not change the population of Bacillus vegetative and spore form in the rhizosphere. Results indicate that BCN can replace conventional NPK 16-16-16 fertilizer for tomato plants in Andisol soil order.
PEMBERDAYAAN MASYARAKAT MELALUI PEMANFAATAN LUMPUR SUSU UNTUK VERMIKOMPOS Harlia, Ellin; Marlina, Eulis Tanti; Setiawati, Mieke Rochimi
Jurnal Abdi Insani Vol 12 No 2 (2025): Jurnal Abdi Insani
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/abdiinsani.v12i2.2171

Abstract

Kecamatan Pangalengan merupakan pusat peternakan sapi perah dan pertanian sayuran di provinsi jawa barat, Indonesia. Produksi susu dari peternak sapi perah sebagian diolah menjadi susu pasteurisasi, yoghurt dan keju oleh PT SKP (PT Susu KPBS Pangalengan). Fokus dari PKM ini adalah limbah padat yang dihasilkan dari Instalasi Pengolahan limbah PT SKP berupa lumpur (sludge) yang berasal dari instalasi pengolah air limbah (IPAL). Karakteristik lumpur susu  mendorong adanya pemanfaatan sebagai pakan cacing yang dipelihara melalui proses vermicompost. Melalui kegiatan Pemberdayaan kemitraan  ini berupaya untuk mengembalikan  lumpur susu kepada pada peternak sapi perah untuk dimanfaatkan sebagai pakan  cacing Lumbricus rubellus dengan media cocopeat, mengurangi pencemaran lingkungan, meningkatkan wawasan peternak tentang sumber pencemaran, dan peternak dapat memperoleh penghasilan tambahan dari penjualan cacing segar, cacing kering dan kascing sebagai  pupuk organic untuk tanaman sayuran. Sosialisasi dengan KPBS dilakukan untuk memperoleh izin pengabdian dan mengedukasi pengolahan limbah peternakan. Pelatihan pemanfaatan lumpur susu sebagai pakan cacing dilakukan, termasuk pemeliharaan dengan media cocopeat. Teknologi pascapanen diterapkan, serta pendampingan dan evaluasi dilakukan. Kegiatan pengabdian kepada Masyarakat mengenai Pemberdayaan Masyarakat Peternak Sapi Perah Melalui Pemanfaatan Lumpur Susu Untuk Vermicompos telah dilaksanakan dengan baik. Perserta sangat antusias dalam menyimak dan tingginya  perhatian  peserta yang ditunjukan pada saat sesi tanya jawab. Kegiatan ini memotivasi masyarakat untuk mengolah limbah dari peternakan sapi perah on farm dan industri pengolahan susu sebagai wujud ekonomi sirkular karena adanya dukungan pemasaran produk menciptakan peternakan sapi perah ramah lingkungan dan berkelanjutan.
PENGARUH PUPUK HAYATI DAN PUPUK ANORGANIK TERHADAP POPULASI BAKTERI ENDOFIT, KANDUNGAN KLOROFIL DAN HASIL TANAMAN PAKCOY (Brassica rapa L) PADA HIDROPONIK SISTEM NFT Herlianti, Anissa Mutiara; Setiawati, Mieke Rochimi; Hindersah, Raginawanti
Agrin Vol 22, No 1 (2018): Agrin
Publisher : Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (205.022 KB) | DOI: 10.20884/1.agrin.2018.22.1.454

Abstract

Hidroponik NFT (Nutrient Film Technique) merupakan salah satu sistem hidroponik dimana lapisannutrisi yang sangat dangkal disirkulasikan melewati akar tanaman. Sistem hidroponik bergantung pada nutrisianorganik sebagai pemasok unsur hara. Penggunaan pupuk hayati diharapkan dapat mengurangi dosis pupukanorganik yang diaplikasikan pada sistem hidroponik agar lebih efisien. Penelitian ini bertujuan untukmengetahui dosis terbaik kombinasi pupuk hayati dan pupuk anorganik dalam meningkatkan populasi bakteriendofit, kandungan klorofil dan hasil tanaman pakcoy pada hidroponik sistem NFT. Penelitian telahdilaksanakan diLaboratorium Biologi Tanah, Fakultas Pertanian Universitas Padjadjaran, Jatinangor,Sumedang.Percobaan ini menggunakan Rancangan Acak Kelompok (RAK) yang terdiri dari empat perlakuanyaitu 100% Pupuk Anorganikdan 100% Pupuk Hayati + Pupuk Anorganik 100%, 75% dan 50%. Variabel yangdiamati adalah tinggi tanaman, populasi bakteri endofit, kandungan klorofil, hasil tanaman. Hasil penelitianmenunjukkan bahwa pemberian 100% pupuk hayati dan 50% pupuk anorganik dapat mengurangi penggunaanpupuk anorganik hingga 50 % dan berpengaruh terhadap peningkatan populasi bakteri endofit, kandunganklorofil dan hasil tanaman pakcoy pada hidroponik sistem NFT.Kata kunci: bakteri endofit, klorofil, pupuk hayati, hidroponik NFT, pakcoyABSTRACTHydroponics NFT (Nutrient Film Technique) is one of the hydroponic systems where superficial layers ofnutrients are circulated through plant roots. Hydroponic system is depended on anorganic nutrient as nutrientssupplier. The use of biofertilizer was expected to reduce the dosage of inorganic nutrient solution on hydroponicsystem. The aims of this research were to find the best dosage combination biofertilizer and inorganic nutrientsolution on the population of endophytic bacteria, chlorophyll content and yield of pakcoy (Brassica rapa L) onhydroponic NFT system. The research has been conducted at Soil Biology Laboratory of Faculty of AgriculturePadjadjaran University, Jatinangor, Sumedang Regency. The design experiment was randomized blockdesignwhich consisted offour combinationthat was 100% inorganic nutrients and 100% biofertilizer + inorganicnutrients 100%, 75% and 50%. The variables observed were plant height, population of endophytic bacteria,chlorophyll content, plant yields. The results showed that 100% biofertilizer and 50% anorganic nutrition canreduce usage inorganic nutrients up to 50% and take effect on population of endophytic bacteria, chlorophyllcontent and yield of pakcoy (Brassica rapa L) on hydroponic NFT system.Keywords: endophytic bacteria, chlorophyll, biofertilizer, NFT hydroponic, pakcoy
Co-Authors . Purwanto Abdul Hasyim Sodiq Abraham Suriadikusumah Adawiyah, Aliya Z Ade Setiawan Adhitiya Rana Adinata, Kustiwa Adinata, Kustiwa Aditya, Fasa Agung Karuniawan Aisyah, Ayu Siti Alia Halimatusy Alin Kusumah Dewi Alyani Shabrina Amalia Chusnul Anas Ramdhani Anggi Jingga Anne - Nuraini ANNE NURBAITY Anne Nurbaity Anni Yuniarti Apong Sandrawati Arafah, Mayang Sunduz Ari Abdulah Safari Asmiran, Priyanka Azwari, Fachruddin Benang Purwanto Benny Joy Benny Joy Benny Joy Betty N. Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Betty Natalie Fitriatin Chotimah, Andina Damayani, Maya Daud Siliwangi Saribun Dedeh H Arief Dedeh H. Arief Dedi Widayat Dewi, Yeni Wispa Diana Nafitri Cahayaningrum Diky Indra Wibawa Dirga Sapta Sara Diyah Sri Utami Diyan Herdiyantoro DIYAN HERDIYANTORO Diyan Herdiyantoro Diyan Herdiyantoro Djuwansah, Muhamad Rachman DWI ANDREAS SANTOSA DWI NINGSIH SUSILOWATI DWI NINGSIH SUSILOWATI Eka Safitri Eko Pranoto Eko Pranoto Eko Pranoto Ellin Harlia Emma Trinurani Sofyan Eulis Tanti Marlina Evi Entang Fatimah Evi Entang Fatimah Fani Fauziah Fani Fauziah, Fani Fatantia Husnaeni Fera Siti Meilani Fiky Yulianto Wicaksono Fitriatin, Betty N Gordon Pius Marihot Halimah, Ummu ‘Azizah Hari R Harlyadi Haryo Probo Kusumo Herdiantoro, Diyan Herlianti, Anissa Mutiara Hersanti - Hindersah, Raginawanti I. Syarifain, Roby Iin Handayani Ilyas, Ichsan Imas Komalasari Indrawati - Indrawati Isna Niar Rahmatul Azizah Jajang Sauman Hamdani Kaffah, Ruhnayati Kamluddin, Nadia Nuraniya Khoiriyyah, Luluatul Khumairah, Fiqriah Hanum Kmaluddin, Nadia Nuraniya Kustiwa Adinata Kustiwa Adinata Leoni Silvia Lia Nur Linda Lubis, Ary Satria Maharani, Nadhira Saniya Mahdi Argawan Putra Mahfud Arifin Manurung, Dahlia Florencia Maulana, Nurzen Maya Damayani Maya Damayanti Mayang Sunduz Arafah Melani . Melani Melani Muhamad Khais Prayoga Muhamad Khais Prayoga Nabila Syifa Ariani Nadia Nuraniya Kamaluddin Nadia Nuraniya Kamluddin Nanda Aditya Setyawan Nandha Afrilandha Neni Rostini Neni Rostini Neni Rostini Nenny Nurlaeny Nida Uli Al-Azmiya Nizar Ulfah Nugraha, Gita Bina Nurmayulis Nurullita Fitri Qurnia Pirda Nurhopipah Prayoga, Muhamad Khais Prayoga, Muhamad Khais Priyanka Asmiran Pujawati Suryatmana Putra Utama Putri Sri Judiani Purba Rachelita, Nadia Rahma Tia Harahap Reginawanti Hindersah Reginawati Hindersah Restu Wulansari Restu Wulansari, Restu Rhazista Noviardi Richard A. Gunawan Ridha Hudaya Ridha Hudaya Rija Sudirja Risanti, Rara Rahmatika Salsabilla, Camilla Shabrina Rahma Fauzia Shinta Nurrizqi Indrayani Silke Stoeber Silmi Rahadiana Putri Stoeber, Silke Stoeber, Silke Sumadi Sumadi Suryatma, Pujawati Susiyanti, Susiyanti Syifa Nabila Kurnia Tessa Novianty Putri Asova Tien Turmuktini Tualar Simarmata Tualar Simarmata Tualar Simarmata Tualar Simarmata Tualar Simarmata Tualar Simarmata Tualar Slmarmata Ummu ‘Azizah Halimah Utami, Deswita Uum Umiyati Wati, Dyah Aditya Yanti Ningtyas, Dewi Nurma Yati Rachmiati Yati Rachmiati, Yati Yori Tridendra Yudith Silfani Yuli Astuti Hidayati Yuliati Machfud Yuyun Sumarni Zaenal Mutaqin Zahra Ilmiyati