Article Per Year (5 Year)
p-Index From 2020 - 2025
2.304
P-Index
This Author published in this journals
All Journal MARINA CHIMICA ACTA Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Elkawnie PROSIDING SEMINAR KIMIA Jurnal Industri Hasil Perkebunan Jurnal Kimia Riset Indonesia Chimica Acta Jurnal Penelitian Pendidikan IPA (JPPIPA) Al-Kimia Indonesian Journal of Chemistry Indonesian Journal of Pure and Applied Chemistry Automotive Experiences Journal of Community Health Provision Molekul: Jurnal Ilmiah Kimia Rafflesia Journal of Natural and Applied Sciences (RJNAS) Journal of Biotropical Research and Nature Technology
Nunuk Hariani Soekamto
Department Of Chemistry, Faculty Of Mathematics And Natural Sciences, Hasanuddin University, Perintis Kemerdekaan Street KM 10, Tamalanrea Makasssar, Indonesia.
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Veterinary

Published : 30 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Molekul: Jurnal Ilmiah Kimia

 1 
Production, Characterization, and Toxicity Test of L-asparaginase from Vibrio alginolyticus Bacterial Symbiont of Green Algae Caulerpa lentillifera Kasturiasih, Ni Putu; Ahmad, Ahyar; Arfah, Rugaiyah A.; Khairunnur, Siti; Soekamto, Nunuk Hariani; Taba, Paulina; Hala, Yusafir; Karim, Harningsih
Molekul Vol 18 No 3 (2023)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2023.18.3.8153

Abstract

L-asparaginase is an enzyme that can be used as an anticancer therapeutic agent. This study aimed to optimize fermentation time for production, optimization, and to test the toxicity of L-asparaginase from Vibrio alginolyticus (V. alginolyticus) bacterial symbiont of green algae Caulerpa lentillifera (C. lentillifera). The activity of L-asparaginase enzyme assay and the toxicity test were done by using the Nessler method and the Brine Shrimp Lethality Test (BSLT) method respectively. The results show L-asparaginase with 66 hours of fermentation time indicates the highest enzyme activity. L-asparaginase mentioned has an optimum enzyme activity of 17.99 U/mL for 30 minutes of incubation time at 37 °C, and pH 7.5. The BSLT results show LC50 value of 17.83 μg/mL indicating the enzyme is bio-toxic with high-level toxicity, which can continue for cytotoxicity tests on cancer cells.
The Potential of Paku Gajah (Angiopteris evecta) as Antitumor Through In Vitro and In Silico Studies Rasyid, Herlina; Asmirah, Asmirah; Firdausiah, Syadza; Arief, Ihsanul; Soekamto, Nunuk Hariani
Molekul Vol 19 No 2 (2024)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2024.19.2.10644

Abstract

ABSTRACT. Paku gajah (Angiopteris evecta) is one of the largest ferns which has been used empirically by the Dayak tribe of Kalimantan, Indonesia as a traditional medicine to treat various diseases, one of which is tumors. This research aims to determine the potential utilization of A. evecta stem extract as an antitumor by secondary metabolites analysis, toxicity and antitumor assay. The methods used in this study were gradual maceration using three solvents (n-hexane, ethyl acetate, and methanol), phytochemical screening, toxicity test, antitumor activity assay with the Alamarblue method, Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, and molecular docking analysis. This study indicated that A. evecta stem extract contained secondary metabolites such as flavonoids, saponins, tannins, and steroids. The ethyl acetate and methanol were found as toxic extracts with LC50 130.67 and 314.31 µg/mL, respectively. In line with the toxicity, the antitumor activity of the ethyl acetate extract was the highest with an IC50 of 240.94 µg/mL and phytochemical analysis revealed the presence of violanthin and angiopteroside in the extract. Molecular docking showed that the binding energy and inhibition constants of violanthin and angiopteroside against receptors were higher than standard ligand F82. The interaction between violanthin and the receptor results five hydrogen-bond (H-bond) with Lys920, Cys919, Asp1046, and Leu840, while the angiopteroside produces four H-bonds with Leu836, Leu834, and Arg831. Keywords: Antitumor, molecular docking, paku gajah (Angiopteris evecta).
Co-Authors . Asmirah A.A. Ketut Agung Cahyawan W Abdul Karim Abdul Wahid Wahab Ahyar Ahmad Ahyar Ahmad Ajuk Sapar Alfian Noor Alfian Noor Alfian Noor Alfian Noor Alfian Noor Alfian Noor Amalia, Hilda Ayu Melvi Arfah, Rugaiyah A. Artania Adnin Tri Suma Astuti, Ayun Dwi Badrawati, Kadek Susi Bahrun Bahrun Beddu Jawahir Erwin Erwin Erwin Erwin Erwin Erwin Felycitae Ekalaya Appa Firdaus Firdaus Firdaus Firdaus Firdaus Firdaus, Firdaus Fitriyanti Jumaetri Sami Fredryk Welliam Mandey Hanapi Usman Harningsih Karim Hasnah Natsir Hasnah Natsir Ihsanul Arief Imran Imran Irmawan, Muhammad Jalifah Latip Kalalinggi, Septaria Yolan Kasturiasih, Ni Putu Khairunnur, Siti Kolo, La La Kalamu, La Yusran La Kolo Labanni, Arniati Maming Maming Marry Garson Muh. Bachri Amran Muhammad Zakir Muhammad Zakir Muhammad Zakir Musa, Bulkis Musrifah Tahar Nasriadi Dali Nursiah La Nafie Paulina Taba Payapo Irfa Apriliyanti Rasyid, Herlina Rosmawaty Rosmawaty S Siswanto Sahidin . Samriani Samriani Selfi Wullur Seniwati Dali Sernita, Sernita St. Fauziah St. Fauziah Suriani Nur, Suriani Syadza Firdausiah Syadza Firdausiah Syahrir, Nur Hilal A. Syarifuddin, Syamsidar Haji Tjodi Harlim Tjodi Harlim Tjodi Harlim Tri Aryono Hadi Tri Aryono Hadi USMAN - Yana Maolana Syah Yusafir Hala Yusuf, Mohammad Taufik Zakaria Zakaria Zakaria