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All Journal HAYATI Journal of Biosciences METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari SIMBIOSIS Biogenesis: Jurnal Ilmiah Biologi KUKILA Advances in Tropical Biodiversity and Environmental Sciences Jurnal Pendidikan dan Sosial Humaniora

SANG KETUT SUDIRGA

Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana

Author-ID : 388150

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Veterinary Other

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Potensi Minyak Atsiri Cananga odorata dan Cymbopogon citratus Dalam Menghambat Pertumbuhan Staphylococcus aureus ATCC 25923 dan Candida albicans ATCC 10231 Secara In Vitro Kadek Wegi Kurnilia; Sang Ketut Sudirga; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p07

Cananga odorata and Cymbopogon citratus are two plant species producing inhibitory compounds in their essential oils. In our current research, the potential of their essential oils to inhibit the growth of Staphylococcus aureus and Candida albicans were investigated. MIC (Minimum Inhibitory Concentration) values of mixture of those essential oils with a ratio of 1:1 on those microbes were also assayed. Bioassays were conducted on Nutrient Agar (NA) or Potato Dextrose Agar (PDA) medium added with various ratios of the two plant species essential oils. Medium added with sterile distilled water served as control. The data obtained were analyzed quantitatively with SPSS software for Windows version 22. When significant different at p<0.05 was indicated, the analysis was continued with Duncan's multiple range test. The result showed that all treatment of essential oils applied effectively eliminated the growth of S.aureus. C.albicans appeared to be relatively resistant to 100% of C.odorata essential oil. Elimination of C.albicans was observed when the essential oil of C.odorata was combined with that of C.citratus. When compared to control, all ratios of essential oils treatments were statistically significant (p<0.05). The MIC values of essential oil mixture a ratio of 1:1 on S.aureus and C.albicans were 0,1% and 2,6% respectively. Keywords: essential oil, Cananga odorata, Cymbopogon citratus, Staphylococcus aureus, Candida albicans

DAYA HAMBAT EKSTRAK KULIT DAUN LIDAH BUAYA (Aloe barbadensis Miller) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 25923DAN Escherichia coli ATCC 25922 Ariyanti Ni Kadek; Darmayasa Ida Bagus Gede; Sudirga Sang Ketut
Jurnal Biologi Udayana Vol 16 No 1 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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This research aimed to determine the inhibited effect of Aloe (Aloe barbadensis Miller) rind extract to the growthof bacteria Staphylococcus aureus and Escherichia coli and the most effective concentration for the resistanceagainst both of bacteria tested. The results showed that the extracts of Aloe rind inhibited the growth of bacteriaStaphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. It was indicated by the formation ofinhibition zones with the largest diameter average occurred concentrations of 100% with the size 11,58 mm forthe Staphylococcus aureus and 6,81 mm in Escherichia coli. The highest concentration inhibited the growth ofStaphylococcus aureus ATCC 25923 was 100% and 75% for the Escherichia coli ATCC 25922.

JENIS-JENIS TUMBUHAN SEBAGAI SUMBER PAKAN JALAK BALI (Leucopsar rothschildi S.) DI DESA PED, NUSA PENIDA, KLUNGKUNG, BALI I Ketut Ginantra; A.A.G. Raka Dalem; Sang Ketut Sudirga; I.G.N. Bayu Wirayudha
Bumi Lestari Journal of Environment Vol 9 No 1 (2009)
Publisher : Environmental Research Center (PPLH) of Udayana University

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A study on plants species as resource of food for Bali Starling (Leucopsar rothschildi Stresemann, 1912) was carried out in Ped Village, Nusa Penida, Klungkung Bali, Indonesia between March-April and between August-September, 2006. Explorations methods were conducted in making inventory of plants species and part of plants as food resource. Direct observations were conducted to observe feeding activity of the starling after release. Results of this study showed that 105 plants species have been identified in Ped village. About 30 species (in varied habitus of trees, shrubs, and herbs) acted as food resources for the starling. Part of plants species which had been used as food resources were fruits and seeds. Besides plants as food sources, insects were also available on the studi site (such as grasshopper, ants, caterpillars, butterflies). In addition there were also small reptiles as source of food for the the bird. Plants availability as source of food supports ex-situ conservations of Bali Starling in Ped village.

PEMANFAATAN TUMBUHAN SEBAGAI OBAT TRADISIONAL DI DESA TRUNYAN KECAMATAN KINTAMANI KABUPATEN BANGLI Sang Ketut Sudirga
Bumi Lestari Journal of Environment Vol 4 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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pemanfaatan

Burung Sebagai Atraksi Ekowisata di Kawasan Pariwisata Nusa Dua, Bali A. A. G. Raka Dalem; I K. Muksin; S. K. Sudirga; I. B. M. Suaskara
Bumi Lestari Journal of Environment Vol 3 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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A study on birds has been conducted in Nusa Dua Tourist Resort area, Bali, betweenJanuary, 1999 and January, 2002. In this study, bird species was identified and theirpotentials for ecotourism-bird watching activities were analysed. From the field observationit was found that the site has a potential to be developed as a bird watching site. It can beseen from the diversity of bird species identified there, especially in the lagoon. In Nusa DuaTourist Resort area 83 bird species has been identified which is representing over 27% ofbird species on Bali. About 87% of those were classified as non-migrant species, while therest were migrant. Among those species, 6% were birds which were known to be restrictedtheir distribution to Indonesia. The strategic location of Nusa Dua Tourist Resort area alsoincreases its potential for development of bird watching activities. For development of theseactivities, holistic management approaches need to be implemented, the approaches of whichneed considering a balance between conservation, economic and empowerment of localcommunity objectives.

PLANTS AS ECOTOUR ATTRACTIONS AROUND TRAIL OF MANGROVE INFORMATION CENTRE IN MANGROVE FOREST OF SOUTHERN BALI I G. A. Sugi Wahyuni; I P. G. Ardhana; S. K. Sudirga; I K. Ginantra
Bumi Lestari Journal of Environment Vol 5 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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A study on plants species sighted around mangrove information centre ofNgurah Rai Mangrove Forest (Tahura Ngurah Rai), Sothern of Bali province(Indonesia), was undertaken in March 2004. The plants sighted on both sides of themangrove trail were listed. Their potential as ecotour attractions was then identified.Results of the study showed that there were 42 plant species identified aroundmangrove trail of this forest. Twelve mangrove species in this ecosystem may act asmajor attractions to the tourists doing tracking there. Information on protected species,the potential use of plants for humans, such as for animal fodder, medicine, the use inancient Balinese community, in traditional way of life of Balinese which is last untilnow, the use for offering in Hindu’s ceremony may also be interesting for tourists.Some plant species may cause problems to tourists who doing trekking so needprecaution, including on those which cause bleeding and disturbance to humanbreathing.

PEMANFAATAN TUMBUHAN SEBAGAI SIMPLISIA OBAT TRADISIONAL DI DESA TRUNYAN Sang Ketut Sudirga
Bumi Lestari Journal of Environment Vol 5 No 1
Publisher : Environmental Research Center (PPLH) of Udayana University

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Research on conservation and the use of plants as sources of traditional medicine wascarried out in Trunyan village. Objectives of this research are as follows: (1) To find outplant species used as traditional medicine; (2) Which parts of plants utilized for medicine; (3)Names of diseases which can be treated, and (4) What action has been taken by the villagersto conserve these plants. The research was carried out for two months within five banjar:Banjar Mukus, Puseh, Madia, Bunut, and Banjar Trunyan. Data were collected throughinterview, on site observation and literature review, and then they were qualitatively as wellas quantitatively analyzed and presented in tables.Results of this research showed that 90 species of plants were identified as materialfor traditional medicine in Trunyan village. Leaves were parts of plants which were usedmost (46.21%), and diseases which can be cured such as coughing, skin diseases, stomachdisorder, rheumatics, hard breathing, fever, and headache, the diseases of which aregenerally not fatal. From the conservation perspective, it was found that very littleproportion (10%) of this species which have been cultivated.

PEMBERIAN EKSTRAK BUAH JUWET (Syzygium cumini L.) TERHADAP JUMLAH DAN MORFOLOGI SPERMATOZOA TIKUS PUTIH (Rattus sp.) JANTAN YANG TERPAPAR ASAP ROKOK Fitria Dwijayanti; A.A.S.A. Sukmaningsih; , Ni Made Rai Suarni; Sang Ketut Sudirga; Ni Made Susun Parwanayoni
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

The research to determine the effect of fruit extracts juwet on the number and morphology of spermatozoa were superbly exposed to smoke has been conducted. This study uses rats aged 3 month, 200-210 gram, total 24 rats consisting of 4 treatment, is control, smoke of cigarette, juwet fruit extracts, and smoke of cigarette to juwet fruit extracts. Each treatment consisting 6 replications, each consisting of 1 rat. Before, acclimatization of rat treated 7 days. Way of giving juwet fruit extracts the gavage method 2 ml once daily for 48 days, while the CMC-Na 0,5% is given control. The exposure to cigarette smoke is given from an aerator pump , to given once daily for 48 days. Data were analyzed with ANOVA, if they were 5% significantly different would be followed by a DMRT. The result showed that the number of abnormal sperm morphology were significantly diferent (p<0,05). The averange number of abnormal spermatozoa K1 group was higher than K3 group. The number of spermatozoa was not significant different between treatments. The average number of spermatozoa K1 lower than K3. This is because the smoke of cigarette increased formation of ROS and the resulting stress oxidative and cause cell damage tissues and organs, especially to the reproductive system. Juwet fruits extract have been able to improve the count and morphologyof rats spermatozoa expose to smoke. This case because of antioxidant from juwet fruit can be protect cell from the free radical attack. Keywords: Rattus sp, smoke of cigarette, java plum fruit, spermatozoa, , ROS, antioxidant.

POTENTIAL OF Pseudomonas sp. AND Ochrobactrum sp. ISOLATED FROM VARIOUS SOIL SAMPLE AS DEGRADING BACTERIA OF HIGH DENSITY POLYETHYLENE (HDPE) AND LOW DENSITY POLYETHYLENE (LDPE) PLASTIC Muhammad Inas Riandi; Retno Kawuri; Sang Ketut Sudirga
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

plastics on a large scale has an effect on the increase to the amount of world waste, such as High Density Polyethylene (HDPE)and Low Density Polyethylene (LDPE) plastics. This study, tried to isolate Pseudomonas and Ochrobactrum bacteria from varioussoil in dumped soil area and mangrove forest in Bali to find out the degradation ability of the bacteria against HDPE and LDPEplastics. The methods used in this study were pour plate method, Biochemical and morphology identification by using Bergey’sManual of Determinative Bacteriology 9th Edition book as a reference, screening test and the determination of bacterial species byusing Vitek 2 Compact System. Evaluation of degradation result was done by measuring the weight loss of plastics residues. Thisstudy has isolated 27 bacteria that estimated from genus Pseudomonas and Ochrobactrum with characteristics as basil shape, Gramnegative, positive catalase and classified as non-fermenter bacteria. Isolate 2 bacteria which isolated from Suwung dumped soil hasidentified as Ochrobactrum anthropi with the ability of HDPE degradation by 20% and Isolate 24 which isolated from Karangasemdumped soil has identified as Pseudomonas aeruginosa with the ability of HDPE degradation by 18,75%.Keywords : Plastics waste, Biodegradation, High Density Polyethylene and Low Density Polyetene

UJI VIABILITAS BENIH ANGGREK HITAM (Coelogyne pandurata Lindl.) DENGAN MASA SIMPAN YANG BERBEDA VEMY CLAUDIA; IDA AYU ASTARINI; SANG KETUT SUDIRGA
SIMBIOSIS Vol I, No 2, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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The black orchid (Coelogyne pandurata Lindl.) is an epiphytic orchid spesies that iscurrently endangered. To protect and maintain the genetic resources it is necessary to find theproper way to store seeds in a long time. The purpose this study is to determine the viability ofthe black orchid seeds sowed in organic medium with different storage period. The result showedthat the seeds sown in fresh condition has the highest percentage of growth up to 60%, seedswith storage period of 2 weeks has growth percentage of 20%, while the storage period of 4weeks has growth percentage of 2.5%. The growth phase of sowed seeds in fresh condition andseeds storage in 2 weeks periode is phase 4 (second leaf formed embryo) and seeds storage in 2weeks periode is phase 2 (green embryo). This suggest that the storage condition of black orchidseeds is influence physiology and seed viability.Keywords: Coelogyne pandurata Lindl., seed viability, storage periode

Co-Authors A.A.S. A. Sukmaningsih Affrina Fauziah Anak A.G.R Dalem Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Ariyanti Ni Kadek Aulia Nurbaiti Barnadus Nggahu Lindi Djawa Candra Dewi Oktaviawati Deglory Tunmuni Dessy Sukma Wirastuti Dessy Sukma Wirastuti Dewa Ngurah Suprapta Fitria Dwijayanti I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I KETUT MUKSIN I P. G. Ardhana I.G.N. Bayu Wirayudha IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara IRIANI SETYAWATI Junita Hardini Kadek Mardika Kadek Wegi Kurnilia MADE SUSUN PARWANAYONI Margareta Dacosta Muhammad Inas Riandi Ngurah Intan Wiratmini Ni Desak Made Aprilia Dewi Ni LUH ARPIWI Ni Made Gari Ni Made Rai Suarni Ni Putu Adriani Astiti Ni Wayan Sudatri Nur Assiam Nyoman Darsini Retno Kawuri Shelly Burgin Silviana Febrionita Meiskia Agung Susun Susun Prawanayoni VEMY CLAUDIA Wafiatul Fitriyah Yan Ramona Yusni Khusnita Pangesti

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