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All Journal HAYATI Journal of Biosciences METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Biogenesis: Jurnal Ilmiah Biologi KUKILA Jurnal Pendidikan dan Sosial Humaniora SIMBIOSIS Metamorfosa: Journal of Biological Science Open Access DRIVERset
SANG KETUT SUDIRGA
Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Veterinary Other

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Potensi Minyak Atsiri Cananga odorata dan Cymbopogon citratus Dalam Menghambat Pertumbuhan Staphylococcus aureus ATCC 25923 dan Candida albicans ATCC 10231 Secara In Vitro Kadek Wegi Kurnilia; Sang Ketut Sudirga; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p07

Abstract

Cananga odorata and Cymbopogon citratus are two plant species producing inhibitory compounds in their essential oils. In our current research, the potential of their essential oils to inhibit the growth of Staphylococcus aureus and Candida albicans were investigated. MIC (Minimum Inhibitory Concentration) values of mixture of those essential oils with a ratio of 1:1 on those microbes were also assayed. Bioassays were conducted on Nutrient Agar (NA) or Potato Dextrose Agar (PDA) medium added with various ratios of the two plant species essential oils. Medium added with sterile distilled water served as control. The data obtained were analyzed quantitatively with SPSS software for Windows version 22. When significant different at p<0.05 was indicated, the analysis was continued with Duncan's multiple range test. The result showed that all treatment of essential oils applied effectively eliminated the growth of S.aureus. C.albicans appeared to be relatively resistant to 100% of C.odorata essential oil. Elimination of C.albicans was observed when the essential oil of C.odorata was combined with that of C.citratus. When compared to control, all ratios of essential oils treatments were statistically significant (p<0.05). The MIC values of essential oil mixture a ratio of 1:1 on S.aureus and C.albicans were 0,1% and 2,6% respectively. Keywords: essential oil, Cananga odorata, Cymbopogon citratus, Staphylococcus aureus, Candida albicans
DAYA HAMBAT EKSTRAK KULIT DAUN LIDAH BUAYA (Aloe barbadensis Miller) TERHADAP PERTUMBUHAN BAKTERI Staphylococcus aureus ATCC 25923DAN Escherichia coli ATCC 25922 Ariyanti Ni Kadek; Darmayasa Ida Bagus Gede; Sudirga Sang Ketut
Jurnal Biologi Udayana Vol 16 No 1 (2012): Jurnal Biologi
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

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Abstract

This research aimed to determine the inhibited effect of Aloe (Aloe barbadensis Miller) rind extract to the growthof bacteria Staphylococcus aureus and Escherichia coli and the most effective concentration for the resistanceagainst both of bacteria tested. The results showed that the extracts of Aloe rind inhibited the growth of bacteriaStaphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. It was indicated by the formation ofinhibition zones with the largest diameter average occurred concentrations of 100% with the size 11,58 mm forthe Staphylococcus aureus and 6,81 mm in Escherichia coli. The highest concentration inhibited the growth ofStaphylococcus aureus ATCC 25923 was 100% and 75% for the Escherichia coli ATCC 25922.
JENIS-JENIS TUMBUHAN SEBAGAI SUMBER PAKAN JALAK BALI (Leucopsar rothschildi S.) DI DESA PED, NUSA PENIDA, KLUNGKUNG, BALI I Ketut Ginantra; A.A.G. Raka Dalem; Sang Ketut Sudirga; I.G.N. Bayu Wirayudha
Bumi Lestari Journal of Environment Vol 9 No 1 (2009)
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on plants species as resource of food for Bali Starling (Leucopsar rothschildi Stresemann, 1912) was carried out in Ped Village, Nusa Penida, Klungkung Bali, Indonesia between March-April and between August-September, 2006. Explorations methods were conducted in making inventory of plants species and part of plants as food resource. Direct observations were conducted to observe feeding activity of the starling after release. Results of this study showed that 105 plants species have been identified in Ped village. About 30 species (in varied habitus of trees, shrubs, and herbs) acted as food resources for the starling. Part of plants species which had been used as food resources were fruits and seeds. Besides plants as food sources, insects were also available on the studi site (such as grasshopper, ants, caterpillars, butterflies). In addition there were also small reptiles as source of food for the the bird. Plants availability as source of food supports ex-situ conservations of Bali Starling in Ped village.
PEMANFAATAN TUMBUHAN SEBAGAI OBAT TRADISIONAL DI DESA TRUNYAN KECAMATAN KINTAMANI KABUPATEN BANGLI Sang Ketut Sudirga
Bumi Lestari Journal of Environment Vol 4 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

pemanfaatan
Burung Sebagai Atraksi Ekowisata di Kawasan Pariwisata Nusa Dua, Bali A. A. G. Raka Dalem; I K. Muksin; S. K. Sudirga; I. B. M. Suaskara
Bumi Lestari Journal of Environment Vol 3 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on birds has been conducted in Nusa Dua Tourist Resort area, Bali, betweenJanuary, 1999 and January, 2002. In this study, bird species was identified and theirpotentials for ecotourism-bird watching activities were analysed. From the field observationit was found that the site has a potential to be developed as a bird watching site. It can beseen from the diversity of bird species identified there, especially in the lagoon. In Nusa DuaTourist Resort area 83 bird species has been identified which is representing over 27% ofbird species on Bali. About 87% of those were classified as non-migrant species, while therest were migrant. Among those species, 6% were birds which were known to be restrictedtheir distribution to Indonesia. The strategic location of Nusa Dua Tourist Resort area alsoincreases its potential for development of bird watching activities. For development of theseactivities, holistic management approaches need to be implemented, the approaches of whichneed considering a balance between conservation, economic and empowerment of localcommunity objectives.
PLANTS AS ECOTOUR ATTRACTIONS AROUND TRAIL OF MANGROVE INFORMATION CENTRE IN MANGROVE FOREST OF SOUTHERN BALI I G. A. Sugi Wahyuni; I P. G. Ardhana; S. K. Sudirga; I K. Ginantra
Bumi Lestari Journal of Environment Vol 5 No 2
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

A study on plants species sighted around mangrove information centre ofNgurah Rai Mangrove Forest (Tahura Ngurah Rai), Sothern of Bali province(Indonesia), was undertaken in March 2004. The plants sighted on both sides of themangrove trail were listed. Their potential as ecotour attractions was then identified.Results of the study showed that there were 42 plant species identified aroundmangrove trail of this forest. Twelve mangrove species in this ecosystem may act asmajor attractions to the tourists doing tracking there. Information on protected species,the potential use of plants for humans, such as for animal fodder, medicine, the use inancient Balinese community, in traditional way of life of Balinese which is last untilnow, the use for offering in Hindu’s ceremony may also be interesting for tourists.Some plant species may cause problems to tourists who doing trekking so needprecaution, including on those which cause bleeding and disturbance to humanbreathing.
PEMANFAATAN TUMBUHAN SEBAGAI SIMPLISIA OBAT TRADISIONAL DI DESA TRUNYAN Sang Ketut Sudirga
Bumi Lestari Journal of Environment Vol 5 No 1
Publisher : Environmental Research Center (PPLH) of Udayana University

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Abstract

Research on conservation and the use of plants as sources of traditional medicine wascarried out in Trunyan village. Objectives of this research are as follows: (1) To find outplant species used as traditional medicine; (2) Which parts of plants utilized for medicine; (3)Names of diseases which can be treated, and (4) What action has been taken by the villagersto conserve these plants. The research was carried out for two months within five banjar:Banjar Mukus, Puseh, Madia, Bunut, and Banjar Trunyan. Data were collected throughinterview, on site observation and literature review, and then they were qualitatively as wellas quantitatively analyzed and presented in tables.Results of this research showed that 90 species of plants were identified as materialfor traditional medicine in Trunyan village. Leaves were parts of plants which were usedmost (46.21%), and diseases which can be cured such as coughing, skin diseases, stomachdisorder, rheumatics, hard breathing, fever, and headache, the diseases of which aregenerally not fatal. From the conservation perspective, it was found that very littleproportion (10%) of this species which have been cultivated.
Pengaruh ekstrak rumput laut (Eucheuma cottoni) terhadap kadar malondialdehid dan spermatozoa tikus (Rattus norvegicus L.) yang diinduksi natrium nitrit Dessy Sukma Wirastuti; A S S A Sukmaningsih; Sang Ketut Sudirga
Jurnal Biologi Udayana Vol 26 No 1 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i01.p10

Abstract

Produk daging olahan adalah salah satu jenis pangan yang mudah mengalami kerusakan. Kerusakan pada produk daging olahan diakibatkan karena adanya pertumbuhan mikroorganisme. Menanggulangi hal tersebut, perusahaan pengolahan daging banyak menambahkan bahan tambahan pangan (BTP) yaitu natrium nitrit (NaNO2). Akumulasi nitrit yang berlebih dalam tubuh dapat menimbulkan efek yang merugikan pada sistem reproduksi. Penelitian oleh Pavlova et al. (2017) menemukan terjadi penurunan berat epididimis dan pengurangan jumlah spermatoa tikus yang diberi paparan NaNO2 tinggi. Rumput laut Eucheuma cottoni mengandung antioksidan yang dapat menetralkan sifat oksidatif yang ditimbulkan oleh NaNO2. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak rumput laut Eucheuma cottoni terhadap kadar Malondialdehid (MDA) dan spermatozoa pada tikus putih (Rattus norvegicus L.) jantan yang diinduksi NaNO2. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari kontrol negatif (K-), kontrol positif (K+), perlakuan dosis 1 (P1), perlakuan dosis 2 (P2) dan perlakuan 3 (P3). Kontrol negatif (K-) diberikan aquades sedangkan kontrol positif diberikan natrium nitrit dosis 22,5 mg/kg BB. Perlakuan dosis 1,2 dan 3 diberikan metode kombinasi natrium nitrit dan ekstrak rumput laut dosis 150 mg/kgBB/hari (P1), 300 mg/kgBB/hari (P2), dan 450 mg/kgBB/hari (P3). Pemberian perlakuan dilakukan selama 45 hari. Adapun variabel penelitian terdiri dari kadar MDA (Malondialdehid) pada serum dan kualitas spermatozoa meliputi integritas membran, viabilitas, motilitas dan morfologi spermatozoa dari tikus putih jantan. Analisis data dilakukan melalui uji statistik One Way Anova dan dilanjutkan dengan uji Duncan. . Hasil penelitian menunjukkan ekstrak rumput laut dosis 150 mg/kgBB mampu menurunkan kadar MDA (Malondialdehid), meningkatkan motilitas, viabilitas, integritas membran dan morfologi spermatozoa secara signifikan.
Gambaran histologi hati dan ginjal mencit (Mus musculus L.) yang diinduksi karbon tetraklorida (CCl4) setelah pemberian ekstrak daun sirsak (Annona muricata L.) Ni Desak Made Aprilia Dewi; Ngurah Intan Wiratmini; Sang Ketut Sudirga
Jurnal Biologi Udayana Vol 26 No 1 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i01.p03

Abstract

Karbon tetraklorida (CCl4) merupakan senyawa kimia yang bersifat toksik, namun masih sering digunakan dalam industri bahan pendingin, alat pemadam kebakaran, dan pestisida. Penggunaan CCl4 secara terus menerus dapat memicu terbentuknya radikal bebas sehingga berpotensi merusak organ hati dan ginjal. Tanaman sirsak (Annona muricata L.) salah satu tanaman yang dapat digunakan sebagai obat alternatif karena mengandung senyawa antioksidan yang dapat mencegah pembentukan radikal bebas. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun sirsak terhadap gambaran histologi hati dan ginjal mencit (Mus musculus L.) yang diinduksi karbon tetraklorida. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 24 ekor mencit jantan yang dikelompokkan menjadi 4 perlakuan yaitu kontrol negatif (K-) diberi larutan Na-CMC 0,5%, kontrol positif (K+) diinduksi CCl4 0,007 mL/20g BB yang dilarutkan dalam minyak kelapa 0,1 mL, dan dua perlakuan yang diinduksi CCl4 0,007 mL/20g BB serta diberi ekstrak daun sirsak dosis 100 mg/kg BB (P1) dan 200 mg/kg BB (P2). Parameter yang diamati pada histologi hati yaitu degenerasi melemak, degenerasi hidropik, nekrosis, kongesti, serta infiltrasi sel radang. Parameter yang diamati pada histologi ginjal yaitu degenerasi melemak, nekrosis, infiltrasi sel radang, dan pembengkakan glomerulus. Hasil penelitian menunjukkan terjadi penurunan kerusakan yang berbeda nyata (p<0,05) pada P1 dan P2 dibandingkan dengan kontrol positif (K+) pada sayatan histologi hati dan ginjal, sehingga dapat disimpulkan bahwa pemberian ekstrak daun sirsak mampu memperbaiki kerusakan hati dan ginjal setelah diinduksi karbon tetraklorida.
Pemberian Ekstrak Etanol Pelepah Batang Pisang Ketip Dapat Meringankan Polycystic Ovary Syndrome pada Tikus Putih Model Kadek Mardika; Ni Wayan Sudatri; Sang Ketut Sudirga
Jurnal Veteriner Vol 23 No 2 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.217 KB) | DOI: 10.19087/jveteriner.2022.23.2.186

Abstract

Polycystic ovary syndrome is a condition that affects the fertility of female reproductive organs. ketip banana pseudostem extract contains flavonoids, saponins, alkaloids, and vitamin C. It is known that ketip banana pseudostem extract can trigger cell proliferation and contain phytoestrogen compound. The purpose of this study was to determine the ability of ketip banana pseudostem extract on length of estrus cycle, histology structure and wight of reproductive organ, and estrogen hormone levels in white rats of PCOS model. White rats female Wistar strain aged 54 days used a total of 25 animals in this study. The treatment was given for 21 days by 1 ml/tail/day with the gavage method. Vaginal smear was began on 5th day of treatment every 12 hours/day for three estrus cycles (15 days). At the end of the study (21th day), the rats were dissected to take their ovaries and blood. The ovaries were weighed and then histological preparation with paraffin method and hematoxylin-Eosin staining. The parameters observed were length of estrus cycle, weight of ovary, number of ovary follicles, as well as estrogen hormone levels. The result of this study showed that ketip banana pseudostem extract can extend proestrus phases and estrus phases, increase estrogen hormone levels, increase corpus luteum number as significantly (p<0.05) on 150 mg/kgBW dosage.
Co-Authors A.A.S. A. Sukmaningsih Affrina Fauziah Anak A.G.R Dalem Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Ariyanti Ni Kadek Aulia Nurbaiti Barnadus Nggahu Lindi Djawa Candra Dewi Oktaviawati Deglory Tunmuni Dessy Sukma Wirastuti Dessy Sukma Wirastuti Dewa Ngurah Suprapta Dwi Ariani Yulihastuti Felisitas Meli Podhi Fitria Dwijayanti I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I KETUT MUKSIN I P. G. Ardhana I.G.N. Bayu Wirayudha IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara IRIANI SETYAWATI Junita Hardini Kadek Dian Lila Sawitri Kumala Kadek Mardika Kadek Wegi Kurnilia Made Ria Defiani MADE SUSUN PARWANAYONI Margareta Dacosta Maulidya Rosa Putri Utama Muhammad Inas Riandi Ngurah Intan Wiratmini Ni Desak Made Aprilia Dewi Ni LUH ARPIWI Ni Luh Putu Nadia Febryanti Ni Made Gari Ni Made Rai Suarni Ni Made Suartini Ni Putu Adriani Astiti Ni Wayan Sudatri Nur Assiam Nyoman Darsini Retno Kawuri Shelly Burgin Silviana Febrionita Meiskia Agung Susun Susun Prawanayoni VEMY CLAUDIA Wafiatul Fitriyah Yan Ramona Yusni Khusnita Pangesti