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SANG KETUT SUDIRGA
Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Energy Environmental Science Immunology & microbiology Veterinary Other

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PERBANDINGAN KANDUNGAN MINYAK ATSIRI TANAMAN SEREH WANGI (Cymbopogon nardus L. Rendle) YANG DITANAM DI LOKASI BERBEDA Margareta Dacosta; Sang Ketut Sudirga; I Ketut Muksin
SIMBIOSIS Vol 5 No 1 (2017)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (315.947 KB) | DOI: 10.24843/JSIMBIOSIS.2017.v05.i01.p06

Abstract

It has been conducted a research that aims to determine the ratio of essential oil content of citronella stalks (Cymbopogon nardus Rendle L.) grown in the lowlands of Denpasar and the highlands of Bedugul. The experiment was conducted by using experimental designs. The parameters measured were the volume and the level of citronella oil produced. The sample preparation of powdered citronella stalks was as much as 200 grams, macerated with 96% ethanol and evaporated with Vacuum rotary evaporator to form condensed extract. The research used quantitative methods. The volume of hydro distillation was to obtain a crude extract of citronella stalks and the identification of active compounds was conducted by using Gas Chromatography / Mass Spectrometry (GC-MS). It can be concluded that the levels of citronella essential oil stalks from the highland of Bedugul was higher than the lowland areas of Denpasar, while the quality of essential oil of the lowland of Denpasar was better than that of the Bedugul highland. The content of secondary metabolites of the results of GC-MS analysis obtained from the essential oils contained in the analyzed crude extract of citronella stalks namely the compound of Selina-6-en-4-ol (2287322), the compound of n-hexadecanoic acid (1238019) and the compound of Driman-8,11-diol from the lowland of Denpasar while the Selina compound-6-en-4-ol (1856137) from the highland of Bedugul. Keywords: Citronella, essential oil content, altitude
PENGARUH DOSIS DAN LAMA PERLAKUAN EKSTRAK DAUN KALIANDRA MERAH (Calliandra calothyrsus Meissn.) TERHADAP STRUKTUR HISTOLOGI GINJAL MENCIT (Mus musculus L.) Nur Assiam; Iriani Setyawati; Sang Ketut Sudirga
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (714.775 KB)

Abstract

This research aims to determine the effect of red calliandra (Calliandra calothyrsusMeissn.) leaf extrac on the histological structure of the kidney of mice (Mus musculus L.).Treatment was administered orally with varying doses. This research used a CompletelyRandomized Design in factorial pattern of two factors, doses (0 or control, 2, 4, and 6 mg/kgBW) and length of treatment (7, 14, and 21 days), so there are 12 combination groups with 3replications of each. Organ was collected on days 8, 15, and 22 to observe histologicalstructure of the kidney. Renal histological observation of edema, Bowman’s spaceconstriction, and protein deposition, showed no correlation between both factors, but a veryreal correlation occurs in the damage of fatty degeneration, hemorrhage, and nucleuspyknotic. Histological observation of glomerular congestion and infiltration of inflammatorycells did not show any correlation between dose and duration of treatment.Keywords: red calliandra, histopathology of kidney, male mice
EFEKTIVITAS EKSTRAK BUNGA KENANGA (Cananga odorata) UNTUK MENGHAMBAT PERTUMBUHAN JAMUR Colletotrichum acutatum PENYEBAB PENYAKIT ANTRAKNOSA PADA TANAMAN CABAI BESAR (Capsicum annum) Silviana Febrionita Meiskia Agung; Sang Ketut Sudirga; Anak Agung Ketut Darmadi
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (212.673 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i01.p10

Abstract

Jenis Colletotrichum acutatum merupakan jamur patogen penyebab penyakit antraknosa pada tanaman budidaya, salah satunya tanaman cabai. Pengendalian penyakit antraknosa umumnya menggunakan pestisida sintetis. Penggunaan pestisida sintetis secara terus menerus dapat menimbulkan efek samping yang merugikan, sehingga diperlukan senyawa lain untuk mengendalikan jamur patogen tersebut salah satunya melalui penggunaan fungisida nabati. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak bunga kenanga dalam menghambat pertumbuhan Colletotrichum acutatum dan untuk mengetahui golongan senyawa aktif yang terkandung dalam ekstrak bunga kenanga. Penelitian menggunakan Rancangan Acak Lengkap (RAL) dengan tujuh perlakuan yaitu P0: kontrol negatif, P1: kontrol positif, P2: konsentrasi ekstrak 1% (b/v), P3: konsentrasi ekstrak 2% (b/v), P4: konsentrasi ekstrak 3% (b/v), P5: konsentrasi ekstrak 4% (b/v), P6: konsentrasi ekstrak 5% (b/v) dengan masing-masing perlakuan diulang sebanyak 4 kali. Parameter yang diamati yaitu terbentuknya diameter zona hambat, diameter koloni, kerapatan spora, dan uji fitokimia ekstrak. Hasil penelitian uji sumur difusi menunjukan zona hambat terbesar terdapat pada konsentrasi 5% (P6) yaitu sebesar 10,05 mm dan zona hambat terkecil pada konsentrasi 1% (P2) sebesar 7,53 mm. Hasil uji koloni menunjukan diameter koloni terkecil pada hari ke-10 terdapat pada konsentrasi 3% (P4) sebesar 7,09 cm dan diameter koloni terbesar pada konsentrasi 1% (P2) sebesar 8,46 cm. Hasil uji kerapatan spora menunjukan jumlah kerapatan spora terbesar terdapat pada konsentrasi 1% (P3) yaitu 3,24×104 spora/mL dan jumlah spora terkecil terdapat pada konsentrasi 3% (P4) sebesar 2,36×104 spora/mL. Golongan senyawa aktif yang ditemukan dalam ekstrak bunga kenanga meliputi alkaloid, steroid, flavonoid, fenolik, saponin dan tanin.
Pengaruh ekstrak rumput laut (Eucheuma cottoni) terhadap kadar malondialdehid dan spermatozoa tikus (Rattus norvegicus L.) yang diinduksi natrium nitrit Dessy Sukma Wirastuti; A S S A Sukmaningsih; Sang Ketut Sudirga
Jurnal Biologi Udayana Vol 26 No 1 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i01.p10

Abstract

Produk daging olahan adalah salah satu jenis pangan yang mudah mengalami kerusakan. Kerusakan pada produk daging olahan diakibatkan karena adanya pertumbuhan mikroorganisme. Menanggulangi hal tersebut, perusahaan pengolahan daging banyak menambahkan bahan tambahan pangan (BTP) yaitu natrium nitrit (NaNO2). Akumulasi nitrit yang berlebih dalam tubuh dapat menimbulkan efek yang merugikan pada sistem reproduksi. Penelitian oleh Pavlova et al. (2017) menemukan terjadi penurunan berat epididimis dan pengurangan jumlah spermatoa tikus yang diberi paparan NaNO2 tinggi. Rumput laut Eucheuma cottoni mengandung antioksidan yang dapat menetralkan sifat oksidatif yang ditimbulkan oleh NaNO2. Penelitian ini dilakukan untuk mengetahui pengaruh ekstrak rumput laut Eucheuma cottoni terhadap kadar Malondialdehid (MDA) dan spermatozoa pada tikus putih (Rattus norvegicus L.) jantan yang diinduksi NaNO2. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang terdiri dari kontrol negatif (K-), kontrol positif (K+), perlakuan dosis 1 (P1), perlakuan dosis 2 (P2) dan perlakuan 3 (P3). Kontrol negatif (K-) diberikan aquades sedangkan kontrol positif diberikan natrium nitrit dosis 22,5 mg/kg BB. Perlakuan dosis 1,2 dan 3 diberikan metode kombinasi natrium nitrit dan ekstrak rumput laut dosis 150 mg/kgBB/hari (P1), 300 mg/kgBB/hari (P2), dan 450 mg/kgBB/hari (P3). Pemberian perlakuan dilakukan selama 45 hari. Adapun variabel penelitian terdiri dari kadar MDA (Malondialdehid) pada serum dan kualitas spermatozoa meliputi integritas membran, viabilitas, motilitas dan morfologi spermatozoa dari tikus putih jantan. Analisis data dilakukan melalui uji statistik One Way Anova dan dilanjutkan dengan uji Duncan. . Hasil penelitian menunjukkan ekstrak rumput laut dosis 150 mg/kgBB mampu menurunkan kadar MDA (Malondialdehid), meningkatkan motilitas, viabilitas, integritas membran dan morfologi spermatozoa secara signifikan.
Gambaran histologi hati dan ginjal mencit (Mus musculus L.) yang diinduksi karbon tetraklorida (CCl4) setelah pemberian ekstrak daun sirsak (Annona muricata L.) Ni Desak Made Aprilia Dewi; Ngurah Intan Wiratmini; Sang Ketut Sudirga
Jurnal Biologi Udayana Vol 26 No 1 (2022): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2022.v26.i01.p03

Abstract

Karbon tetraklorida (CCl4) merupakan senyawa kimia yang bersifat toksik, namun masih sering digunakan dalam industri bahan pendingin, alat pemadam kebakaran, dan pestisida. Penggunaan CCl4 secara terus menerus dapat memicu terbentuknya radikal bebas sehingga berpotensi merusak organ hati dan ginjal. Tanaman sirsak (Annona muricata L.) salah satu tanaman yang dapat digunakan sebagai obat alternatif karena mengandung senyawa antioksidan yang dapat mencegah pembentukan radikal bebas. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun sirsak terhadap gambaran histologi hati dan ginjal mencit (Mus musculus L.) yang diinduksi karbon tetraklorida. Penelitian ini menggunakan Rancangan Acak Lengkap dengan 24 ekor mencit jantan yang dikelompokkan menjadi 4 perlakuan yaitu kontrol negatif (K-) diberi larutan Na-CMC 0,5%, kontrol positif (K+) diinduksi CCl4 0,007 mL/20g BB yang dilarutkan dalam minyak kelapa 0,1 mL, dan dua perlakuan yang diinduksi CCl4 0,007 mL/20g BB serta diberi ekstrak daun sirsak dosis 100 mg/kg BB (P1) dan 200 mg/kg BB (P2). Parameter yang diamati pada histologi hati yaitu degenerasi melemak, degenerasi hidropik, nekrosis, kongesti, serta infiltrasi sel radang. Parameter yang diamati pada histologi ginjal yaitu degenerasi melemak, nekrosis, infiltrasi sel radang, dan pembengkakan glomerulus. Hasil penelitian menunjukkan terjadi penurunan kerusakan yang berbeda nyata (p<0,05) pada P1 dan P2 dibandingkan dengan kontrol positif (K+) pada sayatan histologi hati dan ginjal, sehingga dapat disimpulkan bahwa pemberian ekstrak daun sirsak mampu memperbaiki kerusakan hati dan ginjal setelah diinduksi karbon tetraklorida.
Analysis of Phenolic and Tannin Contents in the Methanol Extract of Sweet and Sour Star Fruit Plants (Averrhoa carambola L) Leaves Commonly Used as Raw Materials of Lawar (A Balinese Traditional Food) Ni Putu Adriani Astiti; Sang Ketut Sudirga; Yan Ramona
Advances in Tropical Biodiversity and Environmental Sciences Vol 3 No 1 (2019): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.334 KB) | DOI: 10.24843/ATBES.2019.v03.i01.p02

Abstract

Lawar, a special Balinese food made of leaves of star fruit plant, is commonly served in any Balinese traditional events, such as temple festival or wedding parties. The leaves of sweet and sour star fruit plant are used to make this traditional food. In this research, phenolic and tannin content of leaves of these types of plant were analyzed with a view to investigate their potential as antioxidant source. Phenolic compound has been well known to have antioxidant activity by neutralizing free radicals and stabilizing singlet oxygen. This compound has capacity to reduce free radicals by chelating metal ions or inhibiting enzymatic system, such as cyclo-oxygenase, mono-oxygenase or xanthine oxidase that catalyze formation of free radicals. [4] reported that plants with high content of phenolic compounds tend to have high nutritious and antioxidant activities Tannin is a compound belongs to polyphenolic group. Such compound is abundantly found in any plants. This compound has capacity to bind proteins, alkaloid, and gelatin. In human body, this compound play a very complex role, such as chelating metal ions, precipitating proteins, and functioning as biological antioxidant. The results of this study showed that the phenol content of sour and sweet star fruit plants were 2830.99/100 mg GAE and 1959.77/100 mg GAE, respectively, while the tannin content of these two plants were 18810.43 mg/100 gr TAE and 11035.75 mg/100 gr TAE, respectively, indicating that the leaves of sour star frit plant had better antioxidant activity than the sweet one.
PEMANFAATAN Trichoderma spp. SEBAGAI BIOKONTROL Sclerotium rolfsii Sacc. PADA TANAMAN KEDELAI (Glycine max L.) Candra Dewi Oktaviawati; Sang Ketut Sudirga; Junita Hardini
SIMBIOSIS Vol 10 No 2 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (273.363 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i02.p07

Abstract

Sclerotium rolfsii Sacc. on soybeans was one of the diseases in the cultivation that caused a decrease in soybeans (Glycine max L.) production. One of the efforts in increasing the growth and the resistance of the soybean was by using the biological agent named Trichoderma spp. This study aimed to control the intensity of the Sclerotium rolfsii that can cause disease in soybeans. this research was conducted from July 2021 to September 2021 at the Biochemical laboratory and at the Green House owned by the Biology Department FMIPA, Udayana University. The research used an completely randomized design (CRD) with five treatments; a) growing media without the treatment (control); b) growing media + 10 g Trichoderma spp., and without S. rolfsii; c) growing media without Trichoderma spp., + 5 g S. rolfsii; d) growing media + 15 g Trichoderma spp., and 5 g S. rolfsii; e) growing media + 20 g Trichoderma spp., and 5 g S. rolfsii. In this research, the treatment was repeated 5 times and 25 combinations of treatments were obtained. Analysis of Variance (ANOVA) was used in this study to analyze the data, it was found that there was a different result <0,05, then it was continued by using the analysis of Post Hoc Duncan Test. According to the research, it was found that there were two different results. Based on in vitro method, it can be concluded that Trichoderma spp. can inhibit S. rolfsii by about 55,56%. Meanwhile, based on in vivo method, it can be concluded that Trichoderma spp. can inhibit S. rolfsii about 40% which the optimal dose of Trichoderma spp. was about 20 g, the highest plant height was 64.4 cm, the highest average dry weight of shoots was 5.1 g, the highest average dry weight of roots was 0.5 g, and the highest average pod weight was 17.0 g.
Pemberian Ekstrak Etanol Pelepah Batang Pisang Ketip Dapat Meringankan Polycystic Ovary Syndrome pada Tikus Putih Model Kadek Mardika; Ni Wayan Sudatri; Sang Ketut Sudirga
Jurnal Veteriner Vol 23 No 2 (2022)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.217 KB) | DOI: 10.19087/jveteriner.2022.23.2.186

Abstract

Polycystic ovary syndrome is a condition that affects the fertility of female reproductive organs. ketip banana pseudostem extract contains flavonoids, saponins, alkaloids, and vitamin C. It is known that ketip banana pseudostem extract can trigger cell proliferation and contain phytoestrogen compound. The purpose of this study was to determine the ability of ketip banana pseudostem extract on length of estrus cycle, histology structure and wight of reproductive organ, and estrogen hormone levels in white rats of PCOS model. White rats female Wistar strain aged 54 days used a total of 25 animals in this study. The treatment was given for 21 days by 1 ml/tail/day with the gavage method. Vaginal smear was began on 5th day of treatment every 12 hours/day for three estrus cycles (15 days). At the end of the study (21th day), the rats were dissected to take their ovaries and blood. The ovaries were weighed and then histological preparation with paraffin method and hematoxylin-Eosin staining. The parameters observed were length of estrus cycle, weight of ovary, number of ovary follicles, as well as estrogen hormone levels. The result of this study showed that ketip banana pseudostem extract can extend proestrus phases and estrus phases, increase estrogen hormone levels, increase corpus luteum number as significantly (p<0.05) on 150 mg/kgBW dosage.
EFFECTIVENESS OF CINNAMON (Cinnamomum burmanni Blume) LEAF EXTRACT IN INHIBITING THE GROWTH OF THE MUSHROOMS Colletotrichum magnum Rossman & Allen CAUSES OF ANTHRACHNOSE DISEASE IN PAPAYA (Carica papaya Linnaeus) Wafiatul Fitriyah; Sang Ketut Sudirga; Ni Made Gari
SIMBIOSIS Vol 11 No 1 (2023)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2023.v11.i01.p09

Abstract

Anthracnose is a disease which is often found attacking plants. This disease can cause dieback or leaf tip damage of mature plants then followed by infection in the fruits, Consequently, this can result in the decrease of productivity. One of the causes of anthracnose is the pathogenic fungus Colletotrichum magnum. This fungus can infect several cultivated plants, one of which is papaya plants. This study aimed to determine the effectiveness of cinnamon leaf extract (Cinnamomum burmanni. Blume) in suppressing the growth of the fungus C. magnum and to analyze the class of compounds contained in cinnamon leaf extract. The method used in this study was a Completely Randomized Design (CRD) with 1 negative control, 1 positive control, and 5 treatments with concentrations of 1%, 2%, 3%, 4%, 5%, each treatment was repeated 4 times and then tested phytochemical screening. The results of the invitro study showed that cinnamon leaf extract was able to inhibit the growth of C. magnum as seen from the MIC test results, namely a concentration of 0.6% with an inhibition zone diameter of 8.37 mm. The results showed that as the concentration of cinnamon leaf extract increased, the diameter of the inhibition zone obtained was getting bigger. what you get is bigger. The concentration of 5% that has the largest inhibition zone among the concentration treatments, which is 19.77 mm. The results of phytochemical screening on cinnamon leaf extract showed that the cinnamon leaf extract contains positive groups of alkaloids, steroids, phenolic compounds, saponins, flavonoids, and tannins. Keyword: anthracnose, Cinnamon leaves, Inhibition, Colletotrichum magnum.
Kandungan vitamin C dan antioksidan lulur bunga telang (Clitoria ternatea L.) Yusni Khusnita Pangesti; Sang Ketut Sudirga; Ni Wayan Sudatri
Jurnal Biologi Udayana Vol 27 No 2 (2023): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2023.v27.i02.p10

Abstract

Kesehatan kulit merupakan salah satu hal yang berhubungan dengan kecantikan yang dapat dirawat dengan menggunakan kosmetik lulur. Lulur dapat dibuat dengan memanfaatkan bahan alami, salah satunya yang memiliki antioksidan almi seperti vitamin C. Tanaman yang berpotensi sebagai bahan lulur adalah kembang telang (Clitoria ternatea L.) karena memiliki kadar vitamin C dan nilai IC50 yang cukup tinggi. Tujuan penelitian ini adalah untuk mengetahui kandungan vitamin C dan antioksidan pada serbuk bunga telang dan sediaan lulur bunga telang, serta mengetahui persepsi responden terhadap lulur bunga telang. Rancangan penelitian yang digunakan untuk uji vitamin C dan antioksidan lulur adalah Rancangan Acak Lengkap (RAL) dengan 6 variasi lulur serta 3 pengulangan. Dilakukan juga uji organoleptik, dan uji hedonik. Uji vitamin C dilakukan dengan metode Titrasi dan uji antioksidan dengan menggunakan metode DPPH. Hasil penelitian dianalisis secara statistika menggunakan uji ANOVA dan apabila terdapat perbedaan nyata maka dilanjutkan dengan menggunakan Uji Duncan. Hasil analisis ditampilkan dalam bentuk tabel. Kandungan vitamin C pada serbuk bunga telang sebesar 1,41 mg/100g dan IC50 sebesar 118,25 ppm dengan kategori sedang. Kandungan vitamin C lulur bunga telang selaras dengan nilai aktivitas antioksidannya dengan hasil tertinggi terdapat pada lulur dengan kombinasi serbuk beras 75% dan serbuk bunga telang 25%, sedangkan terendah pada lulur serbuk beras 95% dan serbuk bunga telang 5%. Lulur yang paling disukai adalah lulur dengan kombinasi serbuk beras 75% dan serbuk bunga telang 25%.
Co-Authors A.A.S. A. Sukmaningsih Affrina Fauziah Anak A.G.R Dalem Anak Agung Gde Raka Dalem Anak Agung Ketut Darmadi Ariyanti Ni Kadek Aulia Nurbaiti Barnadus Nggahu Lindi Djawa Candra Dewi Oktaviawati Deglory Tunmuni Dessy Sukma Wirastuti Dessy Sukma Wirastuti Dewa Ngurah Suprapta Fitria Dwijayanti I Gusti Ayu Sugi Wahyuni I Ketut Ginantra I Ketut Junitha I KETUT MUKSIN I P. G. Ardhana I.G.N. Bayu Wirayudha IDA AYU ASTARINI Ida Bagus Gede Darmayasa Ida Bagus Made Suaskara IRIANI SETYAWATI Junita Hardini Kadek Mardika Kadek Wegi Kurnilia MADE SUSUN PARWANAYONI Margareta Dacosta Muhammad Inas Riandi Ngurah Intan Wiratmini Ni Desak Made Aprilia Dewi Ni LUH ARPIWI Ni Made Gari Ni Made Rai Suarni Ni Putu Adriani Astiti Ni Wayan Sudatri Nur Assiam Nyoman Darsini Retno Kawuri Shelly Burgin Silviana Febrionita Meiskia Agung Susun Susun Prawanayoni VEMY CLAUDIA Wafiatul Fitriyah Yan Ramona Yusni Khusnita Pangesti