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Journal : Jurnal Pengendalian Hayati

Viabilitas Nematoda Entomopatogen Steinernema spp. dalam Media Kombinasi Senyawa Humik - WAGIYANA; Didik SULISTYANTO; Sugeng WINARSO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.328 KB)

Abstract

Its well known, That Entomopathogenic Nematods (EPNs) used as biological agentswhich effective to control pest of Spodoptera litura (Noctuidae: Lepidoptera).The aims of thisresearch were to find out the multifungsion product as well as a biofeltilizer and biopesticide whichsuitable for agriculture sustainability, secondly the materials were to mixed the humic compound andbiological agent Entomopathogenic Nematodes (EPNs). Combination of biopesticide with EPNs asactive ingredient and humic compound as biofertilizer to find out a good product combination whichindicated the highest viability and activity of EPNs Steinernema spp on humic compound, in orderapplication of these meterial more effective and efficient to prepare the soil fertility and pest problem.This research was done with inoculation of EPNs on the medium/culture e.c: phosphate solublebacteria Pseudomonas putida 27.4B, zeolit and the humic compound (liquid culture). Pathogenecitytest of EPNs were done after incubation on the humic compound to the larvae of S. litura.The result of this research showed that long time of viability EPNs only for fourth weeks on the humicculture at (4000 ppm). Actually the EPNs from this incubation could 100 % mortality of the S. lituralarvae after 72 hours inoculation. The infection rate of EPNs on the larvae S. litura was 30,3 tailsduring 24 hours incubation. The viability of EPNs Steinernema spp on the medium with 1000 ppmhumic compound was 20 % after 120 hours and after 4 weeks later all EPNs could not survive on thismedium. It seems, the medium containing zeolith, P. putida 27.4B and humic compound at 1000 ppmwas not suitable medium neither for nematods growth or larvae S. litura survival.
Pemaduan Pseudomonas putida 27.4B dan Trichorderma sp. dalam Media Cair Senyawa Humik yang Diberi Zeolit untuk Mendapatkan Produk Multifungsi Ramah Lingkungan Sugeng WINARSO; Eko HANDAYANTO; SYEKHFANI .; Didik SULISTYANTO
Jurnal Pengendalian Hayati Vol 1 No 1 (2008)
Publisher : Fakultas Pertanian Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.893 KB)

Abstract

Phosphate solubilizing bacteria (Pseudomonas putida 27.4B) is effective in dissolving undissolve P;humik compound can decrease Al exchange in acid soil and can be used as microorganism liquidmedia; Zeolite can improve CEC and bases concentration; and Trichorderma sp. can control diseaseand increase crop yield. Therefore, aim of this research is to make product having multifunction bothenvironment friendly soil amandement (fertilizer) and biopesticide, combination Pseudomonas putida27.4B, humic compound, zeolite, and Trichorderma sp.Potetoes Dextro Agar (PDA) media used to see resistance and growth, continued humic compoundliquid media added zeolite. Research design is completele randomized design with three factor are: 1)Trichorderma sp. concentration are: 0 (control); 104 CFU.ml-1; 105 CFU.ml-1; dan 106 CFU.ml-1; 2)good combination obtained last research are: a). 1000 ppm humic compound; 0,1% zeolit; P. putida27.4B 1012 CFU.ml-1 and b). 5000 ppm humic compound; 0,1% zeolit; P. putida 27.4B 1012 CFU.ml-1; and 3) incubation time are: 2 and 4 weeks.Result of research indicate that combination Pseudomonas putida 27.4B and Trichorderma sp. inPDA media can grow together, with visually speed of Trichorderma sp. growth faster thanPseudomonas putida 27.4B. Pseudomonas putida 27.4B proven can live on, even very good, in humiccompound liquid media added zeolite and Trichorderma sp. Its best growth is at humic compoundconcentration 1000 ppm. Besides that is tendency of combination Pseudomonas putida 27.4B andTrichorderma sp. exactly can increase growth of Pseudomonas putida 27.4B both at humic compoundconcentration 1000 ppm and without addition of humic compound. While at humic compoundconcentration 5000 ppm, Trichorderma sp. inokulasi exactly decrease Pseudomonas putida 27.4B.
Co-Authors - Asmuni Abdullah Taufiq Abdullah Taufiq abdullah Taufiq Achmad Sjaifullah Achmad Sucahyo Agung Budi Santoso Alfarisy, Fariz Kustiawan Ananias De Deus Ancah Caesarina Novi Marchianti Anggriawan, Rendy Ankardiansyah Pandu Pradana Aprilia Rizqi Arifandi, Didin Arum, Ayu Puspita Arum, Ayu Puspita Aryo Fajar Sunartomo Azza Putra Kusuma Baguinon, Nestor Bambang Hermiyanto Basuki, Basuki Bilal Arifin Buot Jr, Inocencio Cacuk Purnomo Damasa Macandog Dedik Budianta Deus, Ananias De Dewi Rokhmah Didik Sulistyanto Didik SULISTYANTO Didik SULISTYANTO Didik SULISTYANTO Didin Arifandi Dwi Wahyuni Dyah Ayu Dyah Ayu Savitri Eko Handayanto Farida Wahyu Ningtyias, Farida Wahyu Fatimatuz Zuhro Fatimatuz Zuhro, Fatimatuz FNU Slameto Ganestri, Rakhmaghfiroh Geonina Hasni Ummul Hasanah Hoesain, Mohammad Hoesain, Mohammad I Indasah IGM Subiksa Iis Nur Asyiah Inocencio Buot Jr Isa Ma’rufi Isa Ma’rui Kacung Hariyono Khrisna Agung Cendekiawan Krismiratsih, Fitri Lintang Mutiara Putri Ma'rufi, Isa Macandog, Damasa Mandala, Marga Marinus H Pandutama Mis war Mochammad Wildan Djatmiko Mochammad Wildan Jadmiko Mochammad Wildan Jadmiko Moh Waisul Karomi Najmi Muhammad Ilmiawan Nanik Kurniati Nestor Baguinon Prasetya Dhanny Prastyantoko Kukuh Pratiwi Ogis Rachel Sotto Rachmi Masnilah Rakhmaghfiroh Geonina Ganestri Rendy Anggriawan Rudy Erwiyono Salsabila Regina Intansari Sheila Natasya Anindia Putri Sholeh Avivi Siti Habibah Siti Habibah Slamerto Slamerto Sofia Sofia Sofia Sofia Sotto, Rachel Sri Hartatik Sudarko Sudarko Sudarko Sudarko Sukron Romadhona Sulistiyowati, Hari Suyono . SYEKHFANI . Syekhfani Syekhfani Syekhfani Syekhfani Tiara Rizky Oceananda Suharto Tri Candra Setiawati Tri Candra Setyawati Wachju Subchan Wachju Subchan Wachju Subchan Wagiyana Wagiyana Widya Irawati Wiwien Sugih Utami Yuli Hariyati Yuli Hariyati Yuliani Elia Yuni Handayani Yunita, Vina Yusfi Afidah