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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Journal of Marine Research Jurnal Kelautan Tropis JURNAL ENGGANO JFMR (Journal of Fisheries and Marine Research) Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Jurnal Ilmiah Multidisiplin

Antonius Budi Susanto

Departemen Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro

Author-ID : 395901

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Other

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Exploration of Plastic-Degrading Bacteria From Marina Beach, Semarang, Central Java Awalina Choirunnisa Rachmawati; Anggara Mahardika; Djohan Djohan; A.B. Susanto; Bibin Bintang Andriana
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 4 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.4.247-253

Plastic waste has threatens the environment and affect to the economic and tourism sectors, marine life, coastal ecosystems and human health. World Wide Fund for Nature (WWF) states that 85% of waste in the oceans is plastic. The Ministry of Environment and Forestry also noted that Indonesia experienced an increase in plastic waste from 14% in 2013 to 16% in 2016. By 2020 the volume of plastic waste in Indonesia predicted to reach 67.8 million tons. Plastic waste takes 100-500 years to completely decompose. An alternative solution is to involve microorganisms to decompose plastic polymers. However, plastic waste reducing bacteria isolated from coastal ecosystem has not been much explored. In this study, an exploration of natural bacteria that degrades plastic waste from coastal ecosystems is carried out. Plastic samples were collected from the Marina Beach Semarang, Central Java. Plastic samples were taken from a depth of 0-10 cm in three coastal ecosystems: coastal sand sediments, rocks and mangroves. Samples then isolated and screened to obtain bacteria that have the potential to degrade polyethylene. Selected bacteria were identified by biochemical physiology according to the method of Cappuccino and Sherman and classified to genus level according to Bergey's Manual of Determinative Bacteriology and Bergey's Manual of Systematic Bacteriology. The results showed that three genera of bacteria had high polyethylene degradation potential with the speed of degradation: Enterobacteriaceae 0.0091%; Moraxella spp. 0.0066%; and Pseudomonas spp. 0.0076% per week.

Exploration, Isolation, and Identification of Carotenoid from Bacterial Symbiont of Sponge Callyspongia vaginalis Iqna Kamila Abfa; Ocky Karna Radjasa; A B Susanto; Handung Nuryadi; Ferry F. Karwur
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 22, No 2 (2017): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

During the past two decades research on marine bacteria has highlighted the tremendous potential of symbiotic-microorganisms as a source of bioactive secondary. One of the potential of the bacterial symbionts is producing a natural pigment, and these organisms can be used as a sustainable source of natural pigments. Carotenoid is one of the most important pigments that has important roles in physiological and molecular processes of microorganisms, as well as for human health. The objective of this study is to analyze carotenoid pigments from marine bacterial symbionts from sponge and to identify bacterial symbionts that produce carotenoid pigments. Pigment analysis was performed by a UV-VIS spectrophotometer and High Performance Liquid Chromatography (HPLC). Molecular bacterial identification was performed based on 16S rDNA sequence. The isolation of bacterial symbionts from C. vaginalison Zobell 2216E medium resulted in one bacterium, CB-SP5, positively synthesized carotenoids. By reverse phase HPLC analysis, the carotenoid pigments in the bacterial symbionts were identified as diadinoxanthin, fucoxanthin, neoxanthin, dinoxanthin, anddiadinochrome. CB-SP5 shared the highest level of 16S rDNA gene sequence similarity with Psychrobacter celer (99%). Keywords : carotenoid, sponge, bacterial symbiont, 16S rDNA.

Studi Persporaan Rumput Laut Gracilaria dari Perairan Jepara A. B. Susanto; Rini Pramesti; Nirwani Nirwani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 7, No 2 (2002): Jurnal Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Gracilaria adalah salah satu genus dari algae merah yang mempunyai potensi ekonomi karena kandungan agar-agarnya. Masyarakat mendapatkan alga ini melalui persediaan di alam atau hasil budidaya. Untuk penyediaan bibit budldaya Gracilaria telah dllakukan dengan teknik penyetekan. Namun sesungguhnya, spora yang mempunyai fungsi sebagai alat reprodukslnya dapat dljadikan sebagai bibit. Penelitian ini dilakukan di laboratorium basah Marine Station di Teluk Awur Jepara. Pengamatan dilakukan pada bulan Juni 1996. Metode yang digunakan adalah eksperimen dengan menggunakan Rancangan Acak Lengkap (untuk pengamatan karpospora) dan Split Plot Design berdasarkan Rancangan Acak Lengkap (untuk pengamatan tetraspora). Pengeringan, periode terang gelap, intensitas sinar dan salinitas digunakan pada eksperimen karpospora (n=15). Perbedaan periode terang gelap, sallnitas dan intensitas sinar telah dilakukan pada eksperimen tetraspora (n=3). Hasil penelltian menunjukkan bahwa pengeringan, periode terang gelap, intensitas sinar dan salinitas memberikan pengaruh nyata pada pelepasan karpospora dengan jumlah terbesar karpospora terlepas dicapai selama 15 menit pengeringan, dibawah 500 lux intensltas sinar, selama 9:15 jam periode terang gelap dan dalam salinitas 20 o/oo Kombinasi perlakuan tersebut juga memberikan pengaruh terhadap pelepasan tetraspora yakni dengan perlakuan 250 lux intensitas sinar; 15:9 jam periode tereng gelap dan salinitas 30o/oo.Kata kunci: Gracilaria, karpospora, salinitas, pengeringan, intensitas sinar, periode terang:gelap Gracilaria is a genus of red algae which has economic potential because of its agar contain. The coastal people collect this alga through natural stock or out door culture. Seeds preparation of Gracilaria culture has been done by grafting technique. But actually, the spores which has function as reproduction organ. can be used as seedling. This research was done at the wet laboratory of Marine Station, Awur Bay, Jepara. The observations were done on June 1996. The method used was an experimental with a Completly Randomized Design (for carpospore observation) and Split Plot Design 'based on Completly Randomized Design (for tetrespores observation). Desiccation. photoperiod, light intensity and salinity were used on carpospore experiment (n= 15). Different of photoperiods, salinities and light intensities were used on tetrasopres experiments (n=3). The result showed that desiccation, photoperiod. light intensity and salinity gave the highly significant effect on the carpospores liberation with highly carpospore released on desiccation for 15 minutes. 500 lux of light tntenstty, 9:15 hours LD of photoperiod and 20 o/oo. of salinity. The combination treatments also gave effect on the tetrespores released i.e. on the treatment 250 lux of light intensity, 15:9 hours LD of photoperiod and 30 o/oo of salinity.Keywords: Gracilaria, carpospores, salinity, desiccation, light intensity, photoperiod

Estimasi Produk Degradasi Ekstrak Kasar Pigmen Alga Merah Kappaphycus alvarezii (Doty) Doty Varian Merah, Coklat, dan Hijau: Telaah Perbedaan Spektrum Serapan Helly de Fretes; A.B. Susanto; Budi Prasetyo; Heriyanto Heriyanto; Tatas H.P. Brotosudarmo; Leenawaty Limantara
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 17, No 1 (2012): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Penelitian ini bertujuan untuk mengestimasi produk degradasi pigmen ekstrak kasar alga merah Kappaphycus alvarezii (Doty) Doty varian merah, coklat dan hijau yang terbentuk selama perlakuan iradiasi dan pemanasan, melalui perbedaan spektrum serapannya. Ketiga varian diekstraksi menggunakan 100% metanol. Uji fotostabilitas ekstrak pigmen dilakukan dengan iradiasi menggunakan lampu Volpi intralux 4100 pada intensitas cahaya 39300 lux, 56700 lux dan 76400 lux. Lama waktu penyinaran 0, 5, 10, 15, dan 20 menit. Sedangkan uji termostabilitas dilakukan pada suhu 25 dan 90 ° C selama 0, 3, 6 24, dan 48 jam. Pola spektra diukur pada panjang gelombang 300-800 nm sebelum dan sesudah perlakuan dengan spektrofotometer UV-Tampak Shimadzu 1700. Data dianalisis dengan SPINA Versi 3, untuk memperoleh intensitas maksimum dan perbedaan spektra serapan. Hasil penelitian menunjukkan bahwa produk degradasi pigmen ekstrak kasar alga merah K. alvarezii dapat diidentifikasi melalui spektrum serapan dan hasil perbedaan spektra serapan. Isomer cis karotenoid, karotenoid tidak berwarna, dan feofitin a diestimasi sebagai produk degradasi yang terbentuk selama perlakuan iradiasi dengan intensitas cahaya 76400 lux dan perlakuan pemanasan pada suhu 90°C selama 48 jam. Penurunan intensitas warna larutan pigmen juga mengindikasikan terbentuknya produk degradasi selama perlakuan. Kata kunci: Kappaphycus alvarezii, perbedaan spektrum serapan, produk degradasi The aims of this study were to estimate the degradation products of crude pigment extracts from red, brown, and green varieties of red alga Kappaphycus alverezii (Doty) Doty that formed during irradiation and heating treatment from their difference absorption spectra. All three variants were extracted using 100% methanol. The photostability assay of crude pigment extracts was conducted by irradiating the crude pigment extracts with a day light lamp (Volpi, Intralux 4100) at 39300 lux, 56700 lux and 76400 lux light intensity during 0, 5, 10, 15, and 20 minutes treatment, whereas the thermostability assay was performed at 25 and 90 °C for 0, 3, 6 24, and 48 hours. The absorption spectra of the crude pigment extract before and after each treatment were monitored successively at 300-800 nm using MultiSpec 1501 UV-VIS spectrophotometer. Data were analyzed with spina Version 3, to obtain maximum intensity and the difference absorption spectra. The results showed that the degradation products of crude pigment extracts from red alga K. alvarezii could be identified by absorption spectra and difference absorption spectra. Cis isomers carotenoids, colorless carotenoids, and feofitin-a were estimated as degradation product formed during the irradiation treatment with 76400 lux of light intensity and heating treatment at a temperature of 90 ° C for 48 hours. Decrease in the intensity of the color of pigment solution also indicates the formation of degradation products during treatment.Key words: Kappaphycus alvarezii, difference absorption spectra, degradation products

Pigments Characterization and Molecular Identification of Bacterial Symbionts of Brown Algae Padinasp. Collected from Karimunjawa Island Damar Bayu Murti; A B Susanto; Ocky Karna Radjasa; Ferdy Semuel Rondonuwu
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 21, No 2 (2016): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The search for carotenoids in nature has been extensively studied because of their applications in foods. One treasure of the biopigment source is symbiotic-microorganisms with marine biota. The advantages of symbiont bacteria are easy to culture and sensitize pigments. The use of symbiont bacteria helps to conserve fish, coral reefs, seagrass, and seaweed. Therefore, the bacteria keeps their existence in their ecosystems. In this study, bacterial symbionts were successfully isolated from brown algae Padina sp. The bacterial symbionts had yellow pigment associated with carotenoids. The pigments were characterized using High Performance Liquid Chromatography (HPLC) with a Photo Diode Array (PDA) detector. The carotenoid pigments in the bacterial symbionts were identified as dinoxanthin, lutein and neoxanthin. Molecular identification by using a 16S rRNA gene sequence method, reveals that the bacterial symbionts were closely related to Bacillus marisflavi with a homology of 99%. Keywords :carotenoid pigments, brown algae, Padina, bacterial symbionts, 16S rRNA

Kandungan Senyawa Beta Karoten pada Spirulina platensis dengan Perlakuan Perbedaan Lama Waktu Pencahayaan Triana Hanani; Ita Widowati; AB Susanto
Buletin Oseanografi Marina Vol 9, No 1 (2020): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Spirulina platensis merupakan salah satu mikroalga yang mengandung pigmen beta karoten. Beta karoten memiliki manfaat sebagai antioksidan dan antikanker. Pertumbuhan mikroalga dipengaruhi oleh berbagai macam faktor lingkungan, salah satunya adalah cahaya. Penelitian ini bertujuan untuk menentukan lama waktu pencahayaan yang baik untuk mengoptimalkan pertumbuhan dan produksi pigmen beta karoten pada S. platensis.Metode yang digunakan adalah eksperimen laboratoris. Mikroalga S. platensis dikultivasi dengan dua perlakuan lama waktu pencahayaan yang berbeda yaitu A: 24 jam Terang dan 0 jam Gelap (24T,0G); dan B: 12 jam Terang dan 12 jam Gelap (12T, 12G). Pertumbuhan sel S. platensis diamati selama 11x24 jam kemudian dipanen. Biomassa basah hasil kultivasi dikeringkan agar mendapat biomassa kering yang akan diekstraksi menggunakan pelarut n-heksana. Ekstrak n-heksana S. platensis kemudian dianalisis kandungan pigmen beta karotennya secara spektrofotometrik.Hasil penelitian menunjukkan bahwa perlakuan perbedaan lama waktu pencahayaan tidakberpengaruh secara nyata terhadap pertumbuhan sel dan kandungan pigmen beta karoten pada S. platensis. Pertumbuhan sel S. platensis dan kandungan beta karoten tertinggi dihasilkan oleh S. platensis yang dikultur pada pencahayaan 24 Terang dan 0 jam gelap (perlakuan A), yaitu berturut-turut 177,62 x 103 sel/mldan0,00183 µg/g. Spirulina platensis is a microalga containing beta carotene pigment. Beta carotene has the benefit of being antioxidants and anticancer. Various environmental factors influence microalgae growth; one of them is light. The purpose of this research is to determine the best lighting duration to optimize the growth and production of beta carotene pigments in S. platensis. The method used in this research was a laboratory experiment. S. platensis was cultivated with two different lighting duration treatments, i.e.A: 24 hours light, 0-hour dark (24T, 0G), and B: 12 hours light, 12 hours dark (12T, 12G). The growth of S. platensis was observed for 11x24 hours and then harvested. The biomass of S. plantesis was then dried and extracted using n-hexane and analyzed for its beta carotene pigment content spectrophotometrically. The result showed that lighting duration treatment had an insignificant effect on cell growth and beta carotene content ofS.platensis. The optimum growth and beta carotene concentration were optimally achieved by S. platensis cell cultivated in 24 hours light and 0 hours dark, i.e. 177,62 x 103 cell/mL, 0,00183 µg/g respectively.

Struktur Komunitas dan Anatomi Rumput Laut di Perairan Teluk Awur, Jepara dan Pantai Krakal, Yogyakarta Rini Pramesti; AB. Susanto; Wilis A Setyati; Ali Ridlo; Subagiyo Subagiyo; Yohanes Oktaviaris
Jurnal Kelautan Tropis Vol 19, No 2 (2016): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Seaweed has ecological benefits as well as economic value. Waters condition of Awur Bay and Krakal Beach supported this plant’s growth. There’s not yet the latest information about the vegetation. Utilization is still limited on some specieses. Tourist and inhabitant’s activities who take this plant would give impact to this plant’s vegetation. Therefore, it’s necessary to have data collecting, monitoring, and controlling at both of location. This research was aim to inventarisasi of seaweed for morphologic and anatomic characteristics at both of location. The research of method is explorative descriptive. The results showed that the amount of seaweed which was found at Awur Bay based on the morphology characteristics consist of two divisions was Chlorophyta (3 species) and Phaeophyta (5 species). Beside that, the amount of seaweed which was found at Krakal Beach based on the morphology characteristics consist of three divisons was Chlorophyta (4 species), Phaeophyta (2 species) and Rhodophyta (11 species). Three types of cell (anatomy) i.e. epidermis, kortex and medulla. The results of seaweed which found at Krakal Beach are density, frequency, cover percentage, important value index, and ecology index was taller than Awur Bay.Rumput laut bermanfaat secara ekologis maupun ekonomis. Kondisi perairan Teluk Awur, Jepara dan Pantai Krakal, Yogyakarta mendukung tumbuhan ini dapat tumbuh. Pemanfaatannya masih terbatas pada jenis tertentu. Aktivitas wisatawan dan penduduk sekitar yang mengambil tumbuhan ini akan berpengaruh sehingga perlu dilakukan penelitian tentang struktur komunitas di kedua lokasi tersebut. Penelitian ini bertujuan untuk mengetahui struktur komunitas, inventarisasi jenis baik secara morfologi dan anatomi. Metode yang digunakan yaitu metode deskriptif eksploratif. Hasil penelitian menunjukkan jumlah jenis rumput laut yang ditemukan di Teluk Awur terdiri dari dua divisi yaitu Chlorophyta (3 jenis) dan Phaeophyta (5 jenis). Jumlah jenis yang ditemukan di Pantai Krakal terdiri dari tiga divisi yaitu Chlorophyta (4 jenis), Phaeophyta (2 jenis) dan Rhodophyta (11 jenis). Tiga jenis sel penyusun thallus yaitu sel epidermis, korteks dan medulla. Struktur komunitas yang ditemukan di Pantai Krakal meliputi kepadatan, frekuensi, persentase penutupan, indeks nilai penting, dan indeks ekologi lebih tinggi daripada di Teluk Awur.

Kandungan Timbal Pada Air, Sedimen, Dan Rumput Laut Sargassum sp. Di Perairan Jepara, Indonesia Ria Azizah; Rotua Malau; AB Susanto; Gunawan Widi Santosa; Retno Hartati; Irwani Irwani; Suryono Suryono
Jurnal Kelautan Tropis Vol 21, No 2 (2018): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Concentration of Lead in the Seawater, Sediment, and the Seaweed Sargassum sp. in Jepara waters, Indonesia The increasing human activities led to an increase in waste disposal which eventually accumulates and decrease the water quality of rivers and seas. One of the pollutant resulted by human activities is heavy metal. The presence of heavy metals in the waters could directly harm the bioorganisms and indirectly affect human health by food chains. The purpose of this research is to investigate the lead content (Pb) in the water, sediment, and seaweed Sargassum sp., as well as to determine the status of pollution in Teluk Awur waters, Jepara. The research was conducted in November 2017 and January 2018 using descriptive method. Sampling sites were decided by Purposive Sampling Method. Analysis of lead content was conducted using AAS (Atomic Absorption Spectrophotometry). The results showed that the lead content of seawater in Teluk Awur, Jepara, was within 0.003 mg/L and not on the level of concern, according to KepMen LH No. 51/2004. The range of lead content in the sediment was 47- 68,35 mg/kg and considered as polluted, according to NRCC GBW07313. The range of lead content in the seaweed Sargassum sp. was 0.22-0.79 mg/kg and has exceeded the quality standard specified by PerBPOM No. 23/2017. Meningkatnya kegiatan manusia menyebabkan peningkatan pembuangan limbah yang pada akhirnya bermuara ke sungai maupun laut, yang mengakibatkan perairan laut menjadi tercemar. Salah satu pencemar akibat aktivitas manusia adalah logam berat timbal yang dapat membahayakan kehidupan organisme perairan secara langsung, maupun tidak langsung terhadap kesehatan manusia. Tujuan penelitian ini adalah untuk mengetahui kandungan logam berat Pb pada air, sedimen, dan rumput laut Sargassum sp. serta untuk mengetahui status pencemaran di Perairan Teluk Awur, Jepara. Penelitian dilakukan pada bulan November 2017 dan Januari 2018. Metode penelitian yang digunakan adalah metode deskriptif. Penentuan lokasi penelitian dilakukan dengan Purposive Sampling Method. Analisis logam berat Pb dilakukan dengan menggunakan AAS (Atomic Absorption Spectrophotometry). Hasil penelitian menunjukan kandungan logam berat Pb di Perairan Teluk Awur, Jepara, pada air laut yaitu 0,003 mg/L dan masih belum tercemar menurut KepMen LH No.51/2004. Kandungan logam berat Pb pada sedimen yaitu berkisar 47 - 68,35 mg/kg dan telah tercemar menurut NRCC GBW07313. Kandungan logam berat Pb pada rumput laut Sargassum sp. yaitu berkisar 0,22 - 0,79 mg/kg dan telah melebihi baku mutu yang ditentukan oleh PerBPOM No. 23 Tahun 2017

DIVERSIFIKASI OLAHAN RUMPUT LAUT DENGAN PERKEMBANGAN DIGITALISASI BERBASIS IOT DI DESA TELUK AWUR, JEPARA Rahma Nimas Healthy Jayanti; Maylinda Dian Puspitasari; Wa Ode Rima Alam Sari Bolu; Zalsabila Sulistiana; Deasy Fitria Utami; Tasya Afifah Ilmiah; Devi Aulia Zeril Oktavia; Muhammad Reza Yanuar Rahman; Antonius Budi Susanto; David Nugroho
Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Vol 4, No 1 (2022)
Publisher : Fakultas Teknik Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar

Sumber daya Desa Teluk Awur yang melimpah pada dasarnya belum dioptimalkan dengan baik untuk menunjang perekonomian Desa Teluk Awur. Sumber daya tersebut salah satunya adalah rumput laut seperti Latoh (Caulerpa sp) dan Krangkam (Sargassum sp). Tujuan dari kegiatan ini adalah untuk meningkatkan pemanfaatan dan pengolahan rumput laut dengan konsep IOT serta meningkatkan pemahaman serta pemanfaatan digital marketing dalam pengembangan usaha yang dilakukan. etode yang digunakan terdiri dari beberapa tahapan antara lain, penyuluhan mengenai Program Diversifikasi Pengolahan Rumput Laut berbasis IOT (Internet of Things), pelatihan pengolahan rumput laut sebagai Produk Olahan Khas Desa Teluk Awur, pelatihan pengenalan fundamental marketing berbasis IOT untuk pemasaran produk, pembentukan Kelompok Usaha Bersama dalam pengolahan produk rumput laut, dan pembentukan warung/kios untuk pemasaran Produk Olahan Khas Teluk Awur. Kegiatan ini telah berhasil menjaring 10 orang dari PKK yang berniat menjalankan program, mengajak partisipasi pemuda-pemudi untuk menjalankan kegiatan pemasaran, penerapan IoT pada kemasan produk, pembuatan media pemasaran serta menjalin mitra dengan BBPBAP Jepara. Kesimpulan yang didapatkan yaitu adanya partisipasi masyarakat khususnya PKK dan pemuda-pemudi dalam kegiatan pengolahan rumput laut sehingga terbentuknya KUBE Seaweed Kartini dan kios suntuk pengembangan usaha, penerapan IoT berupa QR code dan pemasaran secara digital serta terjalinnya mitra dengan BBPBAP Jepara.Kata kunci : olahan rumput laut, IoT, pemasaran, Desa Teluk Awur

Pengaruh Fermentasi Gracilaria verrucosa dengan Penambahan Starter Lactobacillus plantarum pada Profil Metabolit dan Aktivitas Biologisnya Pola Risda Aswita Silitonga; Wilis Ari Setyati; AB Susanto; Mada Triandala Sibero
Journal of Marine Research Vol 11, No 2 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i2.33262

Gracilaria verrucosa merupakan salah satu spesies penting Rhodophyta yang telah banyak dimanfaatkan dalam berbagai industri. Hasil penelitian sebelumnya menunjukkan bahwa G. verrucosa memiliki aktivitas biologis sebagai antioksidan, namun laporan mengenai potensi sebagai antibakteri dan antikanker masih sedikit dilaporkan. Penelitian ini bertujuan untuk mengetahui pengaruh fermentasi menggunakan L. plantarum terhadap aktivitas antibakteri, toksisitas, dan karakteristik metabolit ekstrak rumput laut G. verrucosa dengan waktu fermentasi yang berbeda. Metode dalam penelitian ini adalah preparasi, fermentasi, ekstraksi, uji antibakteri, uji toksisitas, dan karakterisasi metabolit. Uji aktivitas antibakteri dengan metode agar well diffusion terhadap bakteri foodborne disease sedangkan uji toksisitas dengan metode BSLT. Karakterisasi metabolit dilakukan menggunakan KLT dengan eluent kloroform: etil asetat (9:1), serta visualisasi metabolit dengan reagen Dragendorff, dan Ninhidrin. Hasil analisis KLT menunjukkan ekstrak G. verrucosa yang difermentasi menggunakan L. plantarum menghasilkan senyawa yang diduga senyawa alkaloid dan asam amino bebas. Uji aktivitas antibakteri ekstrak G. verrucosa menunjukkan hasil negatif dan tidak memiliki aktivitas antibakteri terhadap bakteri MDR E. coli dan S. typhi. Analisis toksisitas menunjukkan bahwa setiap ekstrak rumput laut dikategorikan toksik terhadap larva A. salina L. dengan nilai LC50 ETA (73,26 μg/mL), EDA (218,09 μg/mL), Fr 24 (316,69 μg/mL), Fr 48 (316,69 μg/mL), dan Fr 72 (316,69 μg/mL). Berdasarkan hasil penelitian menunjukkan bahwa fermentasi menggunakan L. plantarum tidak dapat meningkatkan aktivitas biologis rumput laut G. verrucosa. Gracilaria verrucosa is one of the most important Rhodophyta species that has been widely used in various industries. The results of previous studies showed that G. verrucosa has a weak antioxidant activity, however, reports on its potential as antibacterial and anticancer are still under explored. This study aimed to determine the effect of fermentation using L. plantarum in G. verrucosa’s metabolites and biological activities with different fermentation times. This study consisted of preparation, fermentation, extraction, antibacterial test, toxicity test, and metabolite characterization. The antibacterial activity assay was conducted by agar well diffusion method against foodborne disease bacteria while toxicity test using BSLT method. Metabolite characterization was carried out using TLC with chloroform : ethyl acetate (9:1) as the eluent, spot visualization was conducted by the addition of Dragendorff, and Ninhydrin reagents. The result of TLC analysis showed that G. verrucosa produced alkaloids and amino acids derivative compounds after fermentation. G. verrucosa extracts had no antibacterial activity against MDR E. coli and S. typhi. Toxicity assay showed that each seaweed extract was categorized as toxic to A. salina L. larvae with an LC50value of ETA (73.26 ug/mL), EDA (218.09 ug/mL), Fr 24 (316.69 ug/mL), Fr 48 (316.69 ug/mL), and Fr 72 (316.69 ug/mL). Based on the results of the study showed that fermentation using L. plantarum could not elevate the antimicrobial and toxicity of G. verrucosa.

Co-Authors Ali Ridlo Ananda Arifidyani Andiska, Prismabella Wilis Anggara Mahardika Anggraeni, Novia Antoni Harahap Ardiawan Pandu Romenda Ardiawan Pandu Romenda Atiqoh, Lailatul Awalina Choirunnisa Rachmawati Bibin Bintang Andriana Budi Prasetyo C. Wiencke Chamidy, Ardian Nurrasyid David Nugroho Deasy Fitria Utami Delicia Yunita Rahma Delicia Yunita Rahma Devi Aulia Zeril Oktavia Djohan Djohan Dwi Haryanti, Dwi Dwi Susilaningsih DWI SUSILANINGSIH Eko Nurcahya Dewi Ferdy Semuel Rondonuwu Ferry Fredy Karwur G. O. Kirst Gunawan Widi Santosa Hadi Endrawati Handung Nuryadi Hanisah Hanisah Hanisah, Hanisah Harahap, Antoni Helly de Fretes Heriawan, Fauzan Heriyanto Heriyanto Iqna Kamila Abfa Irwani Irwani Ita Riniatsih Ita Widowati Joitry Silvia Sitompul Kuncorowati, Ananda Tiara Ade Leenawaty Limantara Mada Triandala Sibero Mahendra, Bima Satria Maylinda Dian Puspitasari Muhamad Ihsan Muhammad Reza Yanuar Rahman Munasik Munasik Nirwani Nirwani Ocky Karna Radjasa Paulus Damar Bayu Murti Pola Risda Aswita Silitonga Pradhana, Handhikka Daffa Wira Prayudha, Muhammad Rifky Puspita Sari Harahap Putut Har Riyadi Rahma Nimas Healthy Jayanti Retno Hartati Ria Azizah Rifana Sobari Rifana Sobari Rinawati Siregar Rini Pramesti Rotua Malau Sembiring, Melisa Octaviani Siregar, Rinawati Subagiyo Subagiyo Suryono Suryono Tasya Afifah Ilmiah Tatas H.P. Brotosudarmo Teuku Muhammad Faisal Teuku Muhammad Faisal, Teuku Muhammad Triana Hanani Wa Ode Rima Alam Sari Bolu Wahid Agung Saputra Wicaksono, Dhanang Hari Widianingsih Widianingsih Widodo, Nabila Azzahro Wilis A Setyati Wilis Ari Setyati Windu Merdekawati Yohanes Oktaviaris Zalsabila Sulistiana

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