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All Journal Jurnal Fitopatologi Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Bioeksperimen: Jurnal Penelitian Biologi Jurnal Teknologi Laboratorium Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education JPSCR : Journal of Pharmaceutical Science and Clinical Research Journal of English Education, Linguistic and Literature JPBIO (Jurnal Pendidikan Biologi) Menara Perkebunan SEMAR Journal of Biodiversity and Biotechnology Dedikasi Saintek Jurnal Pengabdian Masyarakat Molekul: Jurnal Ilmiah Kimia Berkala Ilmiah Biologi Biosaintifika: Journal of Biology & Biology Education Journal of Health Research and Technology Bioeksperimen DEDIKASI SAINTEK Jurnal Pengabdian Masyarakat
ARI SUSILOWATI
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary Other

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Sistem Memori dan Pembelajaran pada Mamalia susilowati, ari; Widiyanto, Slamet; Kusindarta, Dwi Liliek; Wijayanti, Nastiti
Berkala Ilmiah Biologi Vol 15 No 3 (2024)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v15i3.17604

Abstract

Memory formation in mammals involves complex stages, from initial learning to long-term storage. This review highlights key memory-related genes and proteins such as SYNGAP1, Arc/Arg3.1, BDNF, FOXP2, COMT, NR3C1, KIBRA, H-Ras, ERK1/2, and other genes. Mammals serve as important models in memory research due to their evolutionary proximity to humans, providing insights into brain structures such as the hippocampus and prefrontal cortex, which are crucial in memory processes. Molecular mechanisms such as transcription, translation, synaptic plasticity, long-term potentiation (LTP), and long-term depression (LTD), as well as neurotransmitters like glutamate, GABA, acetylcholine, dopamine, and serotonin, are comprehensively discussed with a focus on small, large mammals, and humans. Neurotransmitters affect various functions, including emotion, cognition, memory, learning, and motor functions in test animals. Disruptions in neurotransmitter homeostasis have been linked to numerous neurological and neurodegenerative disorders, highlighting the need to understand the complex mechanisms behind memory in mammals. A brief overview of genes and neurotransmitters related to memory and learning will provide insights and considerations in basic neurobiology and biomedical research.
Haematopoietic stemcell transplantation in Kariadi General Hospital Semarang Indonesia Setyowati, Sri; Rakhmawati, Anik; Susilowati, Ari
Journal of health research and technology Vol. 1 No. 1 (2023): Journal of health research and technology
Publisher : Sahabat Publikasi Kuu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58439/jhrt.v1i1.50

Abstract

In Indonesia, bone marrow transplantation is a treatment which not known yet to some people. Kariadi Hospital is the one hospital that do it first. On 1987, it was successful for acute myeloid leukemia case. Bone marrow transplantation is one of therapy used to treat patients with leukemia, severe aplastic anemia, Hodgkin's disease, non-Hodgkin's lymphoma, myeloma multiple, many blood disorder and solid tumor. This study described the patients characteristics who did hematopoietic stem cell transplantation during 2017-2021 at Kariadi General Hospital Semarang. This study is a quantitative research non-experiment with design retrospective analysis. Data was taken with a total sampling are 20 patients. Data analysis by describing the criteria. The results describe the sex of patients consist are 60% male and 40% female. Most range age at 35-44 years amount 30%. The diagnosis is 40% in the case of Myeloma Multiple. Most of transplantation doing an autologous transplant are 80%. It is indicated 90% of malignancy cases. All patient got 100% engraftment, all patients happen neutropenia, thrombocytopenia, oral mucositis, gut disorder, and electrolyte imbalance. Amount 15% are hematuria and 10% are urticaria, Veno Occlusive Disease, sepsis, brain infection and brain hemorrhage. The conclusion is patients who did hematopoietic stem cell transplantation have various adverse events and complications depending on many factors or comorbids. These are age, sex, diagnosis, stage of disease, state of remission, regiment conditioning. Health professionals should upgrade knowledge and skill for treatment of hematopoietic stem cell transplantation.
Polyurethane Degrading Bacteria Isolated from Decayed Teak Wood (Tectona grandis Linn. f.) Aldila, Farah; Susilowati, Ari; Setyaningsih, Ratna
Jurnal Biodjati Vol 4 No 2 (2019): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v4i2.4525

Abstract

Polyurethane is a class of polymers characterized by their substantial tensile strength and high melting points which enables them to be extremely durable. Despite its xenobiotic origin, polyure-thane has been found susceptible to biodegradation by microorgan-isms. The main object of this research was to investigate polyurethane degradation by the bacterium isolated from decayed teak wood (Tec-tona grandis Linn. f.). Polyurethane degrading bacteria were isolated using selective medium in the form of LB (Luria Bertani) with the ad-dition of 0.3% Impranil. Bacterial biodegradation activities shown by the changes of polyurethane structure analyzed by FT-IR spectrosco-py. The bacterial identification was carried out based on observations on the morphological characteristics of the colonies, cell morphology and sequences of 16S rRNA encoding genes. One of the potential iso-late that successfully isolated was K9, which demonstrates the disap-pearance of the 1735/cm peak of the characteristic function urethane in the FT-IR analysis. Analysis of the 16S rRNA encoding gene showed that the potential isolate having 98% similarity index to Bacillus safen-sis strain FO-36b. The activity shown by the isolate suggests that the bacteria could be a promising agent for polyurethane degradation. 
Peningkatan Kemampuan Bahasa Inggris Santriwati di Pondok Pesantren Al-Madienah Jombang Noviana, Iin; Sari, Desy Siswi Anjar; Susilowati, Ari
DEDIKASI SAINTEK Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2023): Agustus 2023
Publisher : Al-Hijrah Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58545/djpm.v2i2.181

Abstract

Bahasa Indonesia merupakan Bahasa persatuan, sedangkan Bahasa Inggris Menjadi Bahasa Asing. Bahasa Inggris adalah Bahasa Internasional yang digunakan untuk komunikasi, memahami buku ilmiah yang ditulis dalam Bahasa Asing, Jurnal, atau Bahasa Pada website di era digital saat ini Tujuan dari Pengabdian kepada Masyarakat ini adalah untuk meningkatkan kemampuan Bahasa Inggris santriwati Pondok Pesantren Al Madienah Jombang. Kegiatan pengabdian menggunakan pendekatan partisipatif yang memberikan peran aktif peserta selama proses dan pelaksanaan kegiatan ini dengan memahami Bahasa Inggris dan mampu berkomunikasi aktif menggunakan Bahasa Inggris. Kegiatan ini dilaksanakan di Pondok Pesantren Al Madienah Denanyar, Kabupaten Jombang dan dihadiri oleh 100 santriwati.  Paserta sangat antusias dengan jalan nya diskusi dan tanya jawab. yang diselenggarakan oleh tim pelaksana. Santriwati diwajibkan untuk menggunakan Bahasa Inggris sebagai komunikasi sehari - hari, sehingga komunikasi dan diskusi berjalan lancar. Santriwati mendapatkan pengalaman baru dalam pemahaman Bahasa Inggris. Kewajiban untuk berbicara Bahasa Inggris membantu proses pelaksanaan pengabdian masyarakat, sehingga para santriwati mampu untuk berkomunikasi menggunakan Bahasa Inggris.
Relationship between altitudes, morphological traits, and biochemical compositions of Coffea canephora Pierre ex A. Froehner in Temanggung, Indonesia Pangestika, Intan Widya; Susilowati, Ari; Purwanto, Edi; Gunawan
Menara Perkebunan Vol. 93 No. 1 (2025): 93(1), 2025
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v93i1.621

Abstract

Among various edaphoclimatic factors influencing coffee bean quality, altitude is one of the primary factors that should be taken into account. This study aims to explore the relationship between altitude, morphological traits, and biochemical composition of robusta coffee in Temanggung Regency, the largest coffee-producing area in Central Java Province, Indonesia. Research sites were purposively selected and categorized into two altitude groups: GS, PS, and GN, located at circa 600 m a.s.l., and GT, WO, and TG, located at circa 900 m a.s.l. A total of 15 morphological traits were observed, consisting of eight vegetative and seven generative traits. Caffeine content was measured following the AOAC procedure using UV-Vis spectrophotometry, while brew acidity was evaluated using a pH meter. Several morphological traits of Temanggung robusta coffee demonstrated significant relationships with altitude. Canopy and stem diameter showed negative correlations with altitude, with correlation coefficients of -0.366 and  -0.408, respectively. Conversely, fruit width               (r = 0.041), bean length (r = 0.049), and bean thickness (r = 0.047) exhibited positive correlations. Regarding biochemical composition, caffeine content stood out by displaying a strong positive correlation with altitude (r = 0.816). Additionally, several morphological traits, including the number of primary branches, the number of productive branches, the number of fruits per bunch, and traits related to fruits and beans, appear to be advantageous for selection and breeding programs. Understanding these relationships provides valuable insights for developing superior Temanggung robusta coffee plants adapted to specific altitudinal conditions.
Fish Diversity and Phylogenetics in the Lakes of Bromo Tengger Semeru National Park Based on eDNA Chuanata, Wilsen; Susilowati, Ari; Budiharjo, Agung; Travis, Justin M. J.
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.22842

Abstract

Indonesia is home to 1,248 species of freshwater fish, which play a vital role in sustaining the ecosystem. Lakes Ranu Pani, Ranu Regulo, and Ranu Darungan, located within the Bromo Tengger Semeru National Park, lack data on freshwater fish. This study aimed to assess fish diversity in these lakes using environmental DNA (eDNA) metabarcoding and to establish phylogenetic relationships among the species present. Water samples were filtered using 0.45 µm mixed cellulose ester filter paper, after which the collected DNA was extracted utilizing a DNA miniprep kit. The 12S rRNA gene was amplified using the MiFish primer, and the amplicon DNA was sequenced with the Illumina MiSeq 2x250 platform. The sequences were aligned using Blast+ for species identification and subsequently analyzed for alpha and beta diversity. A phylogenetic tree was constructed with MEGA12. The findings revealed eight species from five families, with Gambusia affinis being the most abundant. Ranu Pani exhibited low species diversity, while Ranu Regulo and Ranu Darungan displayed moderate diversity. Beta diversity analysis indicated similarities between Ranu Pani and Ranu Regulo, but distinct communities were found in Ranu Darungan. Phylogenetic analysis showed close relationships between species from the Cichlidae and Osphronemidae families, while a more distant relationship was observed with the Cyprinidae family. These baseline data can serve as a reference for future research on developing conservation strategies, monitoring environmental changes, and supporting biodiversity management.
Isolation and Molecular Identification of Indole Acetic Acid-Producing Endophytic Bacteria from Daun Dewa Plant (Gynura divaricata (L.) DC) Setyowati, Ratna Dewi; Pangastuti, Artini; Susilowati, Ari
Bioeksperimen: Jurnal Penelitian Biologi Vol 9, No 2: September 2023
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v9i2.23003

Abstract

Indole acetic acid (IAA) is an auxin hormone that can regulate plant growth and development. Bacteria produce IAA through L-tryptophan metabolism. The purpose of this study was to determine the ability of endophytic bacteria isolated from Daun Dewa plants to produce IAA to increase the germination and growth of tomato seedlings. Isolation of endophytic bacteria was carried out by sterilizing plant surfaces. The production of IAA is done by adding L-tryptophan precursors. The results showed that there were six endophytic bacteria, and three isolates of them have the potential to produce IAA, namely: AD1.1, UD1.1, and UD2.1. The highest IAA concentration was produced by isolate AD1.1. Growth in sprouts is significantly influenced by the concentration of IAA; the higher the concentration of IAA, the longer the shoots and the roots. Based on the 16S rRNA gene sequence, these isolates belong to the genus Klebsiella.
Morphological and genetic identifications of Spirulina based on 16S rRNA gene Dito, Bahana Safiria; Sajidan, Sajidan; Susilowati, Ari; Sidiq, Yasir
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.14879

Abstract

Spirulina is a microalga with spiral or linear trichome shapes that has potential in industrial and food sectors due to its rich nutritional content. Spirulina can live in various aquatic habitats with different environmental conditions. Differences in cultivation environments can affect the morphological characteristics of Arthrospira due to phenotypic plasticity, making morphology-based identification prone to errors. Therefore, molecular identification using the 16S rRNA gene for phylogenetic analysis is necessary. This study aims to identify and analyze the genetic relationships of Spirulina from freshwater and brackish water based on 16S rRNA gene sequences. Freshwater Spirulina samples were taken from Bogor (BGR) and Yogyakarta (MRP), while brackish water Spirulina was taken from Jepara (JPR). DNA extraction was performed using the Zymo Research Quick-DNA Fungal/Bacterial Miniprep Kit, amplification of the 16S rRNA gene was conducted through PCR, followed by DNA sequencing. Phylogenetic analysis was carried out using MEGA11 to construct the phylogenetic tree. The morphology of strains BGR and MRP showed straight and thin filaments, whereas JPR had spiral and relatively long filaments. Sequencing analysis results showed that strains JPR and MRP had the closest homology similarity to Arthrospira sp. IAQUASC-C0001 with percentages of 99.34% and 99.12%, respectively. Meanwhile, Spirulina from BGR had the closest homology similarity to Arthrospira fusiformis strain AICB 668 with a percentage of 99.78%. Phylogenetic analysis indicated that strains BGR, JPR, and MRP are more closely related to Arthrospira than to Limnospira.
Exploration of students' perceptions of science practicals as a bridge to meaningful learning Sidawati, Asriyani Nurro; Susilowati, Ari; Astuti, Dina; Rahmayanti, Dinia Ika; Yulianti, Yuli; Musliman, Acep
JPBIO (Jurnal Pendidikan Biologi) Vol 11, No 1 (2026): April 2026
Publisher : STKIP Persada Khatulistiwa Sintang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31932/jpbio.v11i1.6205

Abstract

Twenty-first-century science education requires not only conceptual mastery but also the development of scientific process skills and critical thinking. Laboratory work plays a central role in achieving these goals; however, its effectiveness is often limited by “cookbook-style” procedures that fail to promote deep cognitive engagement. This study aimed to explore students’ perceptions of science practicums as indicators of Meaningful Learning (Pembelajaran Bermakna/PBM). Using a qualitative approach with an exploratory case study design, this research involved twelve purposively selected Grade VIII students. Data were collected through in-depth interviews, non-participant observation, and documentation, and were analyzed using thematic analysis. The findings indicate that students perceive meaningful learning as conceptual understanding that can be applied to everyday life rather than mere memorization. Science practicums were viewed as an effective means of achieving PBM, particularly due to the quality of teacher guidance, clear instructions, and opportunities for group collaboration. Although limited laboratory equipment and materials were identified as the main constraints, these limitations did not substantially hinder students’ conceptual understanding. The study concludes that strong pedagogical interactions can bridge hands-on and minds-on learning and support the implementation of competency-based curricula.
Formulasi dan Pengujian Aktivitas Antibakteri Sediaan Gel Hand Sanitizer Berbahan Aktif Kitooligosakarida Wardhani, Diah Ayu; Susilowati, Ari; Pangastuti, Artini
JPSCR: Journal of Pharmaceutical Science and Clinical Research Vol 8, No 1 (2023)
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/jpscr.v8i1.64231

Abstract

Kitooligosakarida merupakan derivat dari kitosan yang memiliki sifat antimikroba dan dijadikan bahan aktif dalam cairan hand sanitizer. Hand sanitizer bebas alkohol dapat menghindari terjadinya toleransi bakteri terhadap bahan kimia seperti alkohol dan mengurangi efek buruk alkohol bagi kulit. Penelitian bertujuan untuk mengetahui karakteristik sediaan hand sanitizer kitooligosakarida dan efektivitasnya dalam membunuh mikroba. Serbuk kitooligosakarida ditambahkan dalam formulasi hand sanitizer dengan variasi berat 1 g (formula 1), 1,5 g (formula 2), dan 2 g (formula 3) menggunakan basis NaCMC, propilenglikol, essens, dan metil paraben. Evaluasi stabilitas fisik hand sanitizer berdasarkan warna, tekstur, aroma, pH, homogenitas, dan daya sebar. Uji daya hambat hand sanitizer diuji melalui metode disc diffusion dilanjutkan dengan perbandingan daya hambat melalui pengukuran diameter zona penghambatan dan uji one-way ANOVA. Uji penurunan angka koloni mikroba dengan metode swabbing dan dilanjutkan perbandingan rerata penurunan koloni mikroba melalui uji T berpasangan. Hasil evaluasi stabilitas fisik hand sanitizer memenuhi kriteria gel yang baik. Hasil uji formula 1 memiliki kriteria angka diameter zona hambat yang lemah pada S. aureus (14,33 cm) dan daya hambat sedang pada E. coli (17,33 cm), Formula 2 memiliki angka diameter zona hambat kuat sebesar 21,33 cm pada S. aureus dan 20,33 cm pada E. coli. Formula 3 menghasilkan zona hambat kuat, yaitu 20,67 cm pada S. aureus dan 22,33 cm pada E. coli. Hasil penurunan angka koloni mikroba sediaan hand sanitizer paling besar yaitu formula 3 sebesar 80,22%. Hand sanitizer kitooligosakarida menunjukkan kemampuan penurunan angka koloni mikroba dan dapat digunakan sebagai formulasi alternatif hand sanitizer.
Co-Authors Acep Musliman Adi Ratriyanto Agung Budiharjo Aldila, Farah Aloysius Djalo Anif Nur Artanti, Anif Nur Anselmus Boy Baunsele ARTINI PANGASTUTI Arum Sari, Siti Lusi Bambang Pujiasmanto Chuanata, Wilsen Dewi, Citra Praba Yunita Dhiah Novalina dina Astuti Dito, Bahana Safiria Dwi Liliek Kusindarta Edi Purwanto Endang Yuniastuti Getrudis Wilhelmina Nau Greiner, Ralf Gunawan Hidayati, Muslihah Nur Hidayatullah, Muhammad Eka Hildegardis Missa Ida Rumia Manurung Mkumbe, Baraka Stewart Mohammad Masykuri Nandariyah, Nandariyah Nastiti Wijayanti Ndukang, Sardina Noviana, I'in Noviana, Iin Pambuko, Galih Pangestika, Intan Widya Paramasari Dirgahayu Putri, Rizkya Suhardo Rahmawati, Sri Indah Rahmayanti, Dinia Ika Rakhmawati, Anik Ratna Dewi Setyowati RATNA SETYANINGSIH Rosmaharani, Shanti Sajidan Sajidan Samanhudi Samanhudi Santos, Americo dos Sari, Desy Siswi Anjar Sari, Siti Lusi Arum Saviera, Shavya Sarah Sidawati, Asriyani Nurro Sigit PRASTOWO Slamet Widiyanto Solikah Ana Estikomah Sri Hartati Sri Mulyani Sri Setyowati Sugiyarto Sugiyarto Sulandjari Sulandjari, Sulandjari Suranto Suranto Suranto Sutarno Sutarno Tifani, Athiyyah Jihan TJAHJADI PURWOKO Travis, Justin M. J. Vanessa, Rebecca Venty Suryanti Wardhani, Diah Ayu WIDYA MUDYANTINI Yasir Sidiq Yulianti, Yuli Yunus, Ahmad