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All Journal Meditory : The Journal of Medical Laboratory Jurnal Kesehatan Jakiyah : Jurnal Ilmiah Umum dan Kesehatan Aisyiyah Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Jurnal Info Kesehatan Jurnal teknologi Kesehatan Borneo
Suwandi, Edy
Department Of Medical Laboratory Technology, Poltekkes Kemenkes Pontianak
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health

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The Effectiveness of Chocolate in Reducing the Number of Methicillin-Resistant Staphylococcus aureus Colonies in Rattus norvegicus Edy Suwandi; Ari Nuswantoro; Sugito Sugito; Desi Wahyumarniasari; Muhammad Reza Setiawan; Dinasti Aprillia; Devi Nurfitri Bintang
JURNAL INFO KESEHATAN Vol 19 No 2 (2021): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol19.Iss2.545

Abstract

Chocolate has long been understood to provide positive emotions and a good mood if consumed in moderation. Chocolate contains prebiotics naturally from its constituent ingredients produced during the production process. Prebiotics, frequently oligosaccharides, are substances which cannot be metabolized by the human digestive system but can be employed by a group of bacteria in the gut, understood as probiotics. The positive relationship among them provides benefits for the host in eliminating pathogens. One of the well-known pathogens which frequently cause infection either in the community or in hospitals is methicillin-resistant Staphylococcus aureus (MRSA). Since it was first identified in 1960, MRSA has caused health problems until today. Research conducted on two groups of Rattus norvegicus infected with MRSA and then fed chocolate revealed a decrease in the average number of bacterial colonies on the skin compared to the control group. In the group fed chocolate at a dose of 50 mg/day, the bacterial colonies decreased to 1.28 x 108 CFU/cm2 in 7 days, lower than in the control group (1.46 x 108 CFU/cm2) at the same time. While those fed 75 mg/day chocolate decreased to 2.70 x 107 CFU/cm2 and the three groups were significantly different (0.000<0.05). Prebiotics fermented by probiotics release short-chain fatty acids (SCFA), which compete with the pathogens for attaching to the epithelial wall so that pathogens lose space and nutrients to survive. However, the adverse effect of chocolate may occur because it contains sugar which is a nutrient for bacteria, but if the balance of normal flora and adequate intake of prebiotics are administered, the pathogen could be eliminated.
Daya Hambat Sediaan Deodoran Spray Minyak Atsiri Kulit Jeruk Bali Terhadap Bakteri Staphylococcus aureus Suwandi, Edy; Saputra, Daniel Angkasa; Kamilla, Laila; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1832

Abstract

Deodoran spray adalah sediaan kosmetika yang digunakan untuk menyerap keringat dan menutupi bau badan. Bau badan merupakan masalah yang cukup penting, bau badan ini dapat ditimbulkan karena kurang menjaga kebersihan badan dan adanya aktivitas bakteri Staphylococcus aureus. Jeruk bali memiliki senyawa metabolit sekunder terpenoid. Senyawa metabolit sekunder tersebut dapat dimanfaatkan sebagai agen antibakteri pada sediaan deodoran spray. Tujuan penelitian ini untuk menganalisis perbedaan sediaan deodoran spray minyak atsiri kulit jeruk bali pada konsentrasi 3%, 6% dan 9% dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Penelitian ini berbentuk Quasi Experimental Design, populasi pada penelitian ini adalah sediaan deodoran spray minyak atsiri kulit jeruk bali dengan menggunakan teknik sampling purposive. Sampel yang digunakan adalah sediaan deodoran spray minyak atsiri kulit jeruk bali konsentrasi 3%, 6% dan 9% yang dibuat 9 kali pengulangan pada masing- masing perlakuan, sehingga total sampel yang digunakan untuk 3 kelompok perlakuan tersebut adalah 27 sediaan deodoran spray yang diuji kekuatan antibakterinya dalam menghambat pertumbuhan bakteri Staphylococcus aureus dengan menggunakan metode difusi cakram Kirby-Bauer. Hasil uji daya hambat sediaan deodoran spray minyak atsiri kulit jeruk bali terhadap bakteri Staphylococcus aureus didapatkan rata-rata zona hambat pada konsentrasi 3% sebesar 7,1 mm, konsentrasi 6% sebesar 7,9 mm dan konsentrasi 9% sebesar 8,7 mm. Dari hasil analisis data uji Friedman diperoleh p-value 0,000 < α 0,05. Sehingga terdapat perbedaan antara setiap konsentrasi sediaan deodoran spray minyak atsiri kulit jeruk bali terhadap pertumbuhan bakteri Staphylococcus aureus.
EDUKASI SENAM KAKI DIABETIK TERHADAP PENURUNAN ANKLE BRACHIAL INDEX (ABI) PADA PASIEN DIABETES MILITUS DI DESA LINGGA Fatayati, Imma; Suwandi, Edy; Ikhsan, Bagus Muhammad
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 6 (2025): MAJU : Indonesian Journal of Community Empowerment, November 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i6.1880

Abstract

Diabetes mellitus can lead to various complications, including peripheral circulation disorders indicated by a decrease in the Ankle Brachial Index (ABI), which may result in amputation. Data from Lingga Public Health Center in 2023 recorded approximately 186 people with diabetes, with an increasing trend each year. Prevention of diabetic foot complications can be achieved through education and simple physical activities such as diabetic foot exercises. This community service aimed to improve diabetic patients’ understanding of ABI reduction through diabetic foot exercises in Lingga Village. The methods included education, exercise training, ABI examination, and evaluation of understanding and implementation. The results showed an 85% increase in participants’ understanding of ABI examination and diabetic foot exercises. In conclusion, there was an improvement in patients’ knowledge, which is expected to serve as a promotive and preventive effort to reduce the risk of diabetic foot complications.
Pengaruh Sediaan Deodoran Krim Kombinasi Destilat Daun Sirih Hijau (Piper betle L.) dan Kulit Kayu Manis (Cinnamomum burmannii) Terhadap Pertumbuhan Staphylococcus aureus Mulyani, Elma Tri; Suwandi, Edy; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.2132

Abstract

Cream deodorant is a topical preparation used to reduce body odor caused by Staphylococcus aureus bacteria in sweat glands. The purpose of this study was to determine the effect of a cream deodorant preparation containing a combination of green betel leaf and cinnamon bark at concentration of 10%, 20%, and 30%  on inhibiting the growth of Staphylococcus aureus bacteria. Synthetic deodorants containing alumunium chloralhydrate have the potential to cause negative side effects, including cancer risk. Essential oils from green betel leaf and cinnamon bark contain antibacterial compounds such as betephenol and cinnamaldehyde, which work synergistically as active ingredients. This study used a purposive sampling technique and the well diffusion method on MHA medium. 10 replications were conducted, resulting in 30 samples. The results showed that the inhibition zone at a concentration of 10% is on average 13,4 mm (strong), at a concentration of 20%, it is on average 20.5 mm (very strong) and at a concentration of 30%, it is on average 23,5 mm (very strong). The results of a simple linear regression statistical test showed a significance value (p=0.000 → p < 0.05), indicating a significant effect of concentration on inhibitory power. This study concludes that the cream deodorant preparation containing the combination of green betel leaf and cinnamon bark distillate was effective in inhibiting Staphylococcus aureus, with the highest inhibition zone at a concentration of 30%. This combination has the potential to be developed as a safe, natural antibacterial deodorant ingredient.
Identifikasi Bakteri Escherichia Coli Pada Ulkus Diabetikum Studi di Klink Kanazawa Pontianak Menggunakan PCR gen 16S rRNA Nurhidayatulloh, Ariffialdi; Ramadhani, Natasya Intan; Abidin, Khoirul Rista; Suwandi, Edy; Salsabila, Aisha; Widodo, Kaltri; Andriani, Lulu; Hamidah, Najwa; Wulandari, Tri
Jurnal Laboratorium Khatulistiwa Vol 9, No 1 (2025): November 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i1.1976

Abstract

Diabetes mellitus can lead to complications such as ulcers that are highly susceptible to bacterial infection. Escherichia coli is one of the pathogenic bacteria commonly found in diabetic foot ulcers. Accurate detection of E. coli species is essential to support appropriate infection management, and one of the reliable methods is Polymerase Chain Reaction (PCR) targeting the 16S rRNA gene. This study employed a descriptive design with an accidental sampling technique. Samples were obtained from swab specimens of diabetic foot ulcer patients. The procedures included culture on Eosin Methylene Blue (EMB) agar, Gram staining, bacterial DNA isolation, PCR amplification of the 16S rRNA gene, electrophoresis for visualization of PCR products, and sequencing for species confirmation. Based on the results of the study involving 30 diabetic foot ulcer patients at Kanazawa Clinic Pontianak, culture analysis showed that 4 isolates were positive for Escherichia coli. Gram staining further confirmed the presence of E. coli by demonstrating its characteristic morphology. The PCR results visualized through electrophoresis showed that 4 samples (13.3%) were positive for Escherichia coli based on the 16S rRNA gene, indicated by the presence of DNA bands at approximately 1500 bp. Sequencing analysis further confirmed the isolates as Escherichia coli with 99% similarity. 
Combination of Aloe Vera and Chocolate to Reduce The Number of The Colony of Staphylococcus Aureus Edy Suwandi; Ari Nuswantoro; Sugito Sugito
Jurnal teknologi Kesehatan Borneo Vol 3 No 2 (2022): Jurnal teknologi Kesehatan Borneo
Publisher : POLTEKKES KEMENKES PONTIANAK

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jtkb.v3i2.197

Abstract

Aloe vera (AV) and chocolate are good examples of functional foods because they contain prebiotics that can help the host defend itself against harmful bacterial infections such as Staphylococcus aureus. Many studies have used AV or chocolate alone to help fight infection, but not much is known about using both together. This study aims to count the number of colonies of S. aureus infected on mice’s skin (Mus musculus) after being given AV and cocoa in certain doses per kilogram of body weight. A total of 35 healthy male mice were infected with S. aureus in the superficial area of ​​the nape of the skin. After that, they were divided into seven groups, each consisting of 5 mice, and given the following treatments: given 50 mg/kg BW AV (group A), 50 mg/kg BW brown (B), 50 mg/kg BW combination of AV and chocolate (C), given 75 mg/kg BW AV (D), given 75 mg/kg BW brown (E), given 75 mg/kg BW combination of AV and chocolate (F), and control (without prebiotic administration), K). Mice that were given 75 mg/kg BW AV/chocolate/AV+chocolate had a better ability to reduce the number of bacteria than those that were given 50 mg/kg BW AV/chocolate/AV+chocolate. In addition, rats that were given AV and chocolate together had a better ability to reduce the number of bacteria than those that were given either only AV or chocolate alone. Prebiotics are both found in AV and chocolate but with different substances. This causes mice given a combination of prebiotics to be considered better than those given only one type of prebiotic source.
Antibakteri Deodorant Spray Minyak Atsiri Bunga Cengkeh terhadap Staphylococcus aureus Dhifa Wira Wicaksana; Edy Suwandi; Etiek Nurhayati; Sugito Sugito; Imma Fatayati; Emilda Sari
Jurnal Laboratorium Khatulistiwa Vol 9, No 2 (2026): MEI 2026
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v9i2.2127

Abstract

Body odor is a common problem that can affect personal comfort and social confidence. Understanding the relationship between clove flower essential oil concentration and the inhibition of Staphylococcus aureus growth is important for improving the effectiveness of natural deodorant formulations. This study aimed to analyze the relationship between the concentration of clove flower essential oil deodorant spray and the inhibition of Staphylococcus aureus among samples tested at the laboratory. This study used an experimental design with a total sample of 30 samples. Data were collected using the bacterial culture diffusion method on Mueller Hinton Agar (MHA) media and analyzed using the simple linear regression test with a significance level of p < 0.05. The results showed that 15% of samples demonstrated antibacterial activity. Statistical analysis revealed a significant relationship between clove flower essential oil deodorant spray concentration and inhibition of Staphylococcus aureus growth (p = 0.000). The concentration of clove flower essential oil deodorant spray significantly influences the inhibition of Staphylococcus aureus. Early development and utilization of natural-based deodorant formulations are recommended to improve product effectiveness and safety.
Co-Authors Abidin, Khoirul Rista Andriani, Lulu Ari Nuswantoro Desi Wahyumarniasari Devi Nurfitri Bintang Dhantriviana, Indah Dhifa Wira Wicaksana Dinasti Aprillia Djohan, Herlinda Emilda Sari Emilda Sari Emilda Sari Etiek Nurhayati Etiek Nurhayati Farchan Ananda Rizqial Febrianty, Resty Hamidah, Najwa Herlinda Djohan Hidayatussalihin Hidayatussalihin Ihsan, Bagus Muhammad Ikhsan, Bagus Muhammad Imma Fatayati Indah Dhantriviana Indrawati, Ratih Kamarudin Kamarudin Laila Kamilla Linda Triana Linda Triana Muarofah, Siti Najatun Muhammad Reza Setiawan Mulyani, Elma Tri Natasya Intan Ramadhani Nurhidayattulloh, Ariffialdi Nurhidayatulloh, Ariffialdi P Purwanto Putra, Rengga Khatulistiwa Ramadhani, Natasya Intan Resty Febrianty Reza Setiawan Rizqial, Farchan Ananda Salsabila, Aisha Saputra, Daniel Angkasa Sari, Emilda Siti Najatun Muarofah Slamet Slamet . Sri Tumpuk SUGITO Sugito - Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Sugito Supriyanto Supriyanto Supriyanto Supriyanto Supriyanto Supriyanto Sya’baniar, Luthfiranda Tri Wulandari Wahdaniah Wahdaniah Wahdaniah Wahyudi Widodo, Kaltri