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Diferensial Granulosit Sapi Bali di Dataran Tinggi dan Rendah di Nusa Penida Ni Made Riska Adnyani; Ni Ketut Suwiti; Ni Luh Eka Setiasih
Buletin Veteriner Udayana Vol. 10 No. 1 Pebruari 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (609.432 KB) | DOI: 10.24843/bulvet.2018.v10.i01.p13

Abstract

The aim of this study is to determine the differential granulocytes performed on neutrophils, eosinophils, and basophils in bali cattle which are raised in the highlands and lowlands in Nusa Penida. Blood samples were taken from 60 female bali cattle. Bali cattle blood sample was done randomly using multi-stage sampling. Blood collection was done through a jugular vein using a venoject. Blood smear was done using slide technique immediately, followed by fixation and sample staining using Giemsa. Differential leucocytes were counted using battlement method until 100 cells was found using 1000x magnification. Completely randomized design (CRD) was used for data analysis. The obtained data were analyzed with Independent Samples T-Test. The results showed that a type of maintenance location (highlands and lowlands) had a significant effect on neutrophil, but has not significant effect to eosinophils and basophils. The percentage of bali cattle neutrophils in the highlands is lower than in the lowlands.
Suplementasi Mineral Pada Pakan Sapi Bali Terhadap Diferensial Leukosit Di Empat Tipe Lahan I Putu Cahyadi Putra; Ni Ketut Suwiti; Ida Bagus Komang Ardana
Buletin Veteriner Udayana Vol. 8 No. 1 Pebruari 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

The aims of this research were to know the effect of mineral intake on Balinese cattle that has been fed on four different types of land (rice field, grassland, garden, and forest) towards theleukocytes differential. The research design follows a completely randomized design (CRD) usinga nested pattern on 48 males from Balinese cattle. The cattle were grouped according to four treatments of mineral intake, namely: controls, 2.5 grams, five grams, and 7.5 grams in each type ofland. Furthermore, the blood was taken from the auricularis superficialis vein in the third month after treatment. A blood smear was prepared using the slide method and a sample was stained withGiemsa. The blood smear was examined by leukocytes differential using battlement method with 1000x magnification. Then, the collected data were analyzed using analysis of variance and Cochran test. The research results show that the given treatment of minerals has no change theleukocytes differential. In contrast, the percentage of lymphocytes in the type of garden land haslower than on the type of forest, rice land and grassland, but has no differences the percentage of neutrophils, eosinophils, basophils, and monocytes in the four types of land. The conclusion isgiving to a concentration of 7.5 gram does not change the differential leukocyte Bali cattle.
Pengaruh Pemberian Pegagan (Centella asiatica) terhadap Struktur Mikroskopis Hati Mencit Pasca Infeksi Salmonella typhi Brahma Tusta Bhirawa; Ni Ketut Suwiti; Ni Luh Eka Setiasih; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Centella asiatica (pegagan) merupakan salah satu tanaman obat yang memilikibanyak fungsi, salah satunya adalah sebagai imunostimulator, yang berarti dapatmerangsang tubuh untuk meningkatkan respon imun. Namun efek pegagan terhadapstruktur mikroskopis hati pasca diinfeksi Salmonella typhi (S.typhi) belum pernahdilakukan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak pegagan terhadapstruktur mikroskopis hati mencit yang diinfeksi S.typhi . Penelitian ini menggunakan 24ekor mencit jantan umur 8-12 minggu, yang dibagi menjadi 4 grup. P0 sebagai kontroldiberikan aquades, dan grup P1,P2, dan P3, masing-masing diberikan 125 mg/bb, 250kg/bb mg, 500 mg kg/bb. perlakuan diulang sebanyak 6 kali. Extrak pegagan diberikansecara oral selama 14 hari, pada hari ke 15 seluruh mencit diinfeksi dengan S.typhi,kemudian pada hari ke 14 semua mencit dinekropsi dan hati diambil untuk diprosespembuatan preparat dengan metode pewarnaan hematoxylin eosin (HE). Pengamatandilakukan terhadap gambaran mikroskopis berupa perdarahan, degenerasi vakuola, dannekrosis. Hasil analisis menunjukkan mencit yang tidak diberikan pegagan dengan mencityang diberikan pegagan dosis 125 mg/kg bb., memberikan hasil yang tidak berbeda nyataNamun mencit yang diberikan pegagan dosis 250 mg/kg bb, dan 500 mg/kg bb memberikangambaran mikroskopis yang berbeda nyata.
Karakteristik Fisik Daging Sapi Bali dan Wagyu Ni Ketut Suwiti; Ni Nyoman Citra Susilawati; Ida Bagus Ngurah Swacita
Buletin Veteriner Udayana Vol. 9 No. 2 Agustus 2017
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.282 KB) | DOI: 10.24843/bulvet.2017.09.02.125

Abstract

A research has been done in order to determine a physical characteristics i.e water holding capacity, acidity level (pH), and cooking loss of beef from bali and wagyu cattle which were sold in supermarkets around Denpasar. The sampels used in this study were 15 rib eye part of bali and 15 of the wagyu beef respectively, the sampels from both types were tested on the water holding capacity parameter, the acidity level, and cooking loss. The data were analyzed by qualitative descriptive analysis. The result showed that, the bali beef contained 66.13 % water holding capacity, 5.75 of acidity level and 31.74 % of coking loss, whereas the wagyu beef contained 63.84 % water holding capacity, 5.20 of acidity level and 32.29% of cooking loss.
Pengaruh Pemberian Pegagan (Centella asiatica) terhadap Gambaran Mikroskopis Limpa Mencit yang Diinfeksi Salmonella typhi I Gede Oka Budiawan; Ni Ketut Suwiti; I Putu Suastika; I Nengah Kerta Besung
Buletin Veteriner Udayana Vol. 5 No.1 Pebruari 2013
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak pegagan terhadap gambaran histologis limpa mencit yang diinfeksi Salmonella typhi. Mencit sebanyak 24 ekor dibagi empat kelompok, yakni kelompok I (P0) sebagai kontrol diberikan aquades steril, kelompok II (P1) diberikan pegagan dengan dosis 125 mg/kg bb, kelompok III (P2) diberikan pegagan 250 mg/kg bb, dan kelompok IV (P3) diberikan pegagan 500 mg/kg bb. Setiap perlakuan diulang sebanyak 6 kali. Pegagan di berikan setiap hari selama 14 hari. Setelah 14 hari seluruh mencit diinfeksi S.typhi. Pada hari ke-15 dilakukan nekropsi untuk pengambilan sampel berupa limpa dan dibuat preparat histologi. Pengamatan preparat dilakukan di Laboratorium Histologi, meliputi persentase nekrosis. Metote pewarnaan menggunakan Haematoxylin Eosin (HE). Data yang diperoleh dianalisis dengan Uji Kruskal-Wallis. Hasil analisis menunjukan gambaran  mikroskopis limpa mencit yang diberikan pegagan dosis 500 mg/kg bb berbeda nyata (P<0,05) dengan limpa mencit yang diberikan pegagan dosis 125 mg/kg bb, 250 mg/kg bb dan mencit yang tidak diberikan pegagan.  
Profil Mineral Magnesium dan Tembaga Serum Darah Sapi Bali yang Dipelihara di Lahan Tegalan Ni Komang Dian Sri Sujani; I Wayan Piraksa; Ni Ketut Ketut Suwiti
Buletin Veteriner Udayana Vol. 6 No. 2 Agustus 2014
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

This study aims to determine the mineral magnesium and copper profile in blood serum of bali cattle farmed on dry land. Samples blood serum was taken from 15 bali cattles that farmed on dry land at Kecamatan Tejakula, Buleleng-Bali. Serums was analyzed by using wet destruction was H2SO4 and HNO3. Samples are read using by Atomic Absorpsion Spectrophotometry (AAS). The result shows that magnesium mean of 22,364 mg/l + 0, 005 mg/l, and copper mean of 0,087 mg/l + 0.001 mg/l. If this result compared to other cattle around the world, magnesium level is still in the normal range, while copper level more lower than normal level.
STUDI HISTOLOGI USUS BESAR SAPI BALI Ni Ketut Suwiti; Ni Luh Eka Setiasih; I Putu Suastika; I Wayan Piraksa; Ni Nyoman Werdi Susari
Buletin Veteriner Udayana Vol. 2 No. 2 Agustus 2010
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

A Study to detect the microscopic structure of large intestine (caecum, colon, rectum) ofbali cattle has been carried out. The samples were collected from 20 of bali cattle in Pesanggaran abattoir, Denpasar. The tissue samples were fixed, dehydrated and embeddedin paraffin and 4-5 mm sections. Harris-Haematoxilin-Eosin staining method, using toidentified of histological structure. Microscopic analysis was performed using binocularlight microscope (100 x, 450x). The study showed that the wall of the caecum, colon, andrectum are made up of four layers : mucosae, submucosae, muscularis externa, and serosa.The lined by mucosae ephitelium of simple columnar cells. The thick of mucosaare 29,9 ± 5,0 mm (caecum) 37,5 ± 8,4 mm (colon) and 58,9 ± 9,5 mm (rectum)respectively. The submucosae contain connective tissue and thick of these layerare 54,8 ± 8,8 mm; 21,2 ± 7,1 mm; and 16,5 ± 4,5 mm respectively. The muscularis externaconsists of two layers of smooth muscle inner circular and outer longitudinal. The thick ofthe mucularis externa are 98,5 ± 15,4 mm; 166,9 ± 44,4 mm; and 479 ± 28,6 mmrespectively. The serosa forms the outermost layer with thick 29,5 ± 8,5 mm;20,9 ± 7,6mm; and 12,1 ± 3,6 mm respectively. We observed for the presence richof Goblet cells and few limphatic nodules (Peyer’s patches) but no showed villi.
Pengaruh Pemberian Pegagan (Centella asiatica) terhadap Gambaran Mikroskopis Usus Halus Mencit yang Diinfeksi Salmonella typhi I Md Chandra Arya PW; I Wayan Piraksa; I Nengah Kerta Besung; Ni Ketut Suwiti
Buletin Veteriner Udayana Vol. 4 No.2 Agustus 2012
Publisher : The Faculty of Veterinary Medicine, Udayana University

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Abstract

Telah dilakukan penelitian mengenai pengaruh pemberian pegagan (Centella asiatica)terhadap gambaran mikroskopis usus halus mencit yang diinfeksi Salmonella typhi.Penelitian ini menggunakan sampel usus halus mencit jantan strain Balb/C yang berumur 2-3 bulan dengan berat badan berkisar antara 20-35 gram. Sebanyak 24 ekor mencit dipakaidalam penelitian, dibagi empat kelompok, yakni kelompok 1 sebagai kontrol diberikanaquades steril, kelompok 2 diberikan pegagan dengan dosis 125 mg/kg bb, kelompok 3diberikan pegagan 250 mg/kg bb, dan kelompok 4 diberikan pegagan 500 mg/kg bb.Setelah 14 hari seluruh mencit diinfeksi dengan S. typhi. Pada hari ke-15 dilakukannekropsi untuk pengambilan sampel berupa usus halus dan dibuat preparat histology,menggunakan pewarnaan Hematoxilin-Eosin. Selanjutnya dilakukan pengamatan diLaboratorium Histologi Fakultas Kedokteran Hewan, terhadap gambaran mikroskopis yangmeliputi perubahan berupa : perdarahan, infiltrasi sel radang, dan deskuamasi epitel usushalus. Metode pewarnaan menggunakan Hematoxilin-Eosin. Data yang diperoleh dianalisisdengan Uji Kruskall-Wallis. Hasil penelitian menunjukkan pemberian pegagan dengandosis 125 mg/kg bb dengan kontrol tidak berbeda nyata (p>0,05), apabila dibandingkandengan dosis pegagan250 mg/kg bb memberikan hasil yang berbeda nyata (p<0,05) danantara pemberian dosis pegagan 250 mg/kg bb dan 500 mg/kg bb memberikan hasil yangtidak berbeda nyata (p>0,05). Hal ini berarti, pemberian ekstrak pegagan (Centella asiatica)dengan dosis 250 mg/kg bb dapat mencegah perubahan gambaran mikroskopis usus halusakibat infeksi S. typhi.
TOTAL BAKTERI SAPI BALI PADA BERBAGAI UMUR DAN LOKASI PETERNAKAN DI NUSA PENIDA chyntia nirmalasari mantrawan; I Nengah Kerta Besung; I Gusti Ketut Suarjana; Ni Ketut Suwiti
Buletin Veteriner Udayana Vol. 10 No. 2 Agustus 2018
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.749 KB) | DOI: 10.24843/bulvet.2018.v10.i02.p03

Abstract

This study aims were to determine the total number of bacteria at maturity level and farm locations. As many as 24 catlle fecal samples were examined using the casting method. This study used a Randomized Complete Block Design of 2 x 3 factorial consist of two farm locations (highlands and lowlands) and three levels of cattle maturity (calves, heifers, adult). The total bacteria were analyzed using Analysis of Variance and continued by Least Significant Difference Test. The results of the total number bacteria isolated from feces adult 724.6250 x 109 CFU/g higher than heifers 667.5000 x 109 CFU/g and calf 499.5000 x 109 CFU/g. Total number of bacteria isolated from feces of bali cattle from highland 629.4167 x 109 CFU/g is not showed significant different compare to bali cattle from lowland 631.6667 x 109 CFU/g.
Kadar Mineral Kalsium Dan Besi Pada Sapi Bali Yang Dipelihara Di Lahan Persawahan (LEVEL OF MINERAL CALCIUM AND IRON ON THE BALI CATTLE BEING FARMEDIN THE RICE FIELD) Ni Nyoman Tri Pujiastari; Putu Suastika; Ni Ketut Suwiti
Buletin Veteriner Udayana Vol. 7 No. 1 Pebruari 2015
Publisher : The Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.296 KB)

Abstract

This research aims were to know the levels of calcium and iron mineral in blood serum of balicattle rared on the rise fields. The sample used in this study were blood serums which were takenfrom 15 bali cattles  in District of Tabanan, Bali Province.  Serum samples were analysed by wetdestruction method, used H2SO4 and HNO3 and mineral concentration are read by AtomicAbsorpsion  Spectrofotometry (AAS). The result show that the level of bali cattle calcium mean of6,23±0,019 mg/l and iron  mean 8,387±0,002 mg/l. The conclusion of this research is the level ofcalcium was lower and iron level was higher, campared to the other cattle in the world.
Co-Authors Anak Agung Ananda Septiarini Anak Agung Gde Arjana Anak Agung Sagung Indraswari Anak Agung Sagung Kendran Anjelia Martina Dewi Antaprapta, I Gusti Ngurah Agung Bianca Violanda Junus Brahma Tusta Bhirawa Chandra Yowani chyntia nirmalasari mantrawan Fedik Abdul Rantam Franki Remi Andung I Gede Agus Eva Prawira Adinata I Gede Erik Juliarta I Gede Oka Budiawan I Gede Semarabawa I Gede Soma I Gusti Agung Ayu Suartini I Gusti Ketut Suarjana I K. Berata I Ketut Suatha I Komang Gde Bendesa I Made Kardena I Made Wima Cahyadi I Md Chandra Arya PW I Nengah Kerta Besung I Nyoman Suarsana I Nyoman Suartha I P. Suastika I Putu Cahyadi Putra, I Putu Cahyadi I Putu Indra Parmayoga I Putu Juli Sukariada I Putu Sampurna I Putu Suastika I W BUDIARSA SUYASA I Wayan Gunawan I Wayan Masa Tenaya I Wayan Piraksa I Wayan Suardana I Wayan Sudira Ida Ayu Pasti Apsari Ida Bagus Komang Ardana Ida Bagus Ngurah Swacita IK Sudibia IM Suyana Utama IW. Piraksa K.K Agustina Ketut Tono Pasek Gelgel Komang Ariya Hendrayana Komang Yogie Suryana Putra Luh Gde Sri Surya Heryani Luh Kadek Nanda Laksmi M Windhu M. Oenas Adinugroho Mergayanti Yudanta Eka Putri Muh Amiruddin N. Suparta N.N. Suryani Ni Kadek Rahayu Swari Ni Komang Dian Sri Sujani Ni Luh Eka Setiasih Ni Luh Putu Sriyani Ni Luh Putu Wiardani Astuti Ni Luh Sri Sundari Rahayu Ni Luh Watiniasih Ni Made Riska Adnyani Ni Made Riska Adnyani Ni Nyoman Citra Susilawati Ni Nyoman Tri Pujiastari Ni Nyoman Werdi Susari Ni Putu Dewi Setia Sari Ni Wayan Ayu Rukmini Ni Wayan Ayu Rukmini Ni Wayan Suryanadi Ni Wayan Tatik Inggriati Nyoman Sadra Dharmawan Oktavyan Loys Mami P Sampurna P. Suastika Putu Agus Trisna Kusuma Antara Putu Ayu Santika Putu Dian Purnama Putra Putu Henrywaesa Sudipa Putu Satya Dwipartha Putu Suastika Rasdianah Rasdianah Reny Navtalia Sinlae Sibang, I Nengah Anom Adi Nugraha Sri Milfa Tri Ulfah Arema Yanti Widodo Cipto Subagyo Widyasanti, Ni Wayan Helpina Winda Ara Yulisa