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All Journal HAYATI Journal of Biosciences Jurnal Ilmiah Farmasi Natural Science: Journal of Science and Technology Jurnal Farmasi Sains dan Komunitas Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Adi Widya : Jurnal Pengabdian Masyarakat JSFK (Jurnal Sains Farmasi & Klinis) Sciences of Pharmacy Jurnal Pengabdian Masyarakat Bangsa Jurnal Pengabdian Farmasi dan Sains Journal of Tropical Pharmacy and Chemistry Jurnal Sains dan Kesehatan
Armini Syamsidi
Jurusan Farmasi, Fakultas MIPA, Universitas Tadulako, Palu, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : HAYATI Journal of Biosciences

 1 
Overproduction, Purification and Refolding of codon-optimized Hepatitis B Virus X Protein Subgenotype B3 in Escherichia coli BL21(DE3) Anita Artarini; Armini Syamsidi; Anindyajati Anindyajati; Raymond R. Tjandrawinata; Debbie S. Retnoningrum
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.164-170

Abstract

Hepatitis B virus (HBV) infects human and causes chronic liver infection, leading to liver cirrhosis and hepatocellular carcinoma. HBV X (Hbx) protein is known to interact with tumor suppressor protein p53 and block its translocation into the nucleus. This study outlines the overproduction of Hbx protein from HBV subgenotype B3 in Escherichia coli BL21(DE3), including its purification and refolding. The gene encoding Hbx was first codon-optimized and inserted into pET16b. The recombinant plasmid was then transformed into E. coli BL21(DE3) as an expression host. Optimization of Hbx expression was performed with variation of IPTG concentration and overproduction temperature. The results showed that Hbx protein was optimally induced by 0.075 mM IPTG and overproduction of Hbx at 17, 25, and 37°C exhibited no difference in protein level and location. The optimal refolding of Hbx was obtained using 0.1 M arginine prior to elution from Nickel column using 100 mM imidazole and 0.25 M arginine. Hbx migrates differently in SDS-PAGE reducing and non-reducing, while the melting curve pattern in TSA analysis changed after the refolding step. Essentially, this purified Hbx protein could potentially be used for interaction study with p53 and the inhibitor candidate of the protein.
Isolation of Endophytic Fungi from Rui (Harrisonia perforata (Blanco) Merr.) and Determining Their Antibacterial, Antioxidant, and Cytotoxic Activity Anam, Syariful; Syamsidi, Armini; Tunreng, Muh. Fadil Usman; Djaleha, Helena Fransisca; Arisca, Windhy Nur; Syaputra, Gunawan; Iklima; Indriani, Meri Feki; Widodo, Agustinus; Pratiwi, Ritha; Yuliet; Razak, Abd. Rahman
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.443-456

Abstract

Endophytic fungi produce bioactive metabolites that exhibit antibacterial, antioxidant, and anti-cancer activity. Rui (Harrisonia perforata (Blanco) Merr.) is a traditional medicinal plant proven to be an antibacterial and anticancer. Endophytic fungi live in plant tissues without damaging or producing chemicals that infect the host cell. Endophytes produce the same and similar compound as an original plant. This study aimed to isolate the endophytic fungi from Rui and determine their antibacterial, antioxidant, and cytotoxic activity. 13 fungal isolates were successfully isolated using PDA for seven days at 30°C. Molecular identification using the ITS1 and ITS4 DNA sequences only revealed six species: Diaporthe sp., Phomopsis sp., Aspergillus tubingensis, Aspergillus viridinutans, Pseudofusicoccum sp., and Daldinia eschscholtz. Seven isolates showed antibacterial activity based on plate assay. Aspergillus tubingensis and RA-1 exhibited antibacterial activity against Staphylococcus aureus and Salmonella typhi, with the MIC and MBC starting from 24 to 32 mg/ml. The DPPH assay showed the most increased antioxidant activity in Daldinia eschscholtzii with an IC50 of 98.14±2.39 µg/ml. The Brine Shrimp Lethality Test (BSLT) showed the highest potential cytotoxic activity, as shown by Aspergillus tubingensis and Phomopsis sp. with LC50 of 7.78±7.48 and 30.83±0.39 µg/ml. In conclusion, our study demonstrates that the fungal extract from Rui could be a source of antibacterial, antioxidant, and cytotoxic agents.
Co-Authors Abd. Rahman Razak Abd. Rasyid Syamsuri Agustinus Widodo Akhmad Khumaidi Anindyajati Anindyajati Anita Artarini Arisca, Windhy Nur Arni Fitriyanti Asriana Sultan Bahar, Zulhaerana Debbie S. Retnoningrum Djaleha, Helena Fransisca Evi Sulastri Friskilla Friskilla Iklima Imanuel Al Jultri Indriani, Meri Feki Ismail, Ihwan Khildah Khaerati Muhammad Sulaiman Zubair Musyahidah Musyahidah Nandiska Maladjili Nela Sharon Nurul Ambianti Nuur Aanisah Pramita Putri Pramita Putri Primadia, Rizki Putri, Ayu Dinawati Raymond R. Tjandrawinata Reyhan Fiqram Rinaldi Rinaldi Ritha Pratiwi Sri Sulistiana Sulaiman , Sri Sulistiana Sulaiman, Sri Sulistiana Syamsuddin, Alifah Magfirah Syaputra, Gunawan Syariful Anam Tunreng, Muh. Fadil Usman Yuliet Yuliet Yusriadi Yusriadi