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All Journal Jurnal Sain Veteriner Buletin Peternakan ILMU KELAUTAN: Indonesian Journal of Marine Sciences AL KAUNIYAH Journal of Natural Sciences and Mathematics Research Jurnal Medik Veteriner ARSHI Veterinary Letters JURNAL KAJIAN VETERINER Jurnal Kedokteran Hewan The International Journal of Tropical Veterinary and Biomedical Research Current Biomedicine Journal of BioSustainability
Nugraha, Arifin Budiman
Division Of Parasitology And Medical Entomology, School Of Veterinary Medicine And Biomedical Sciences, IPB University, Bogor, 16680, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Veterinary Other

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Eksplorasi Parasit pada Ikan Nila (Oreochromis niloticus) dari Penjual Ikan Lokal di Dramaga, Bogor Azizah, Hanifah Nur; Nugraha, Arifin Budiman; Juniantito, Vetnizah; Cahyaningsih, Umi
JURNAL KAJIAN VETERINER Vol 10 No 2 (2022): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v10i2.7973

Abstract

The demand for tilapia fishing goods has recently expanded on a national scale. One of the difficulties this fish farming operation encountered was the parasite-caused illness. The aims of this study were to explore parasites in tilapia (Oreochromis niloticus) from the local fish seller in Dramaga, Bogor, West Java. A total of 14 tilapia were sampled, with an average length of 22 cm and a body weight of 195 grams. Fish gills, pectoral fins, caudal fins, tail fins, dorsal fins, scales, and intestines were collected. The methods used in this study were wet mount examination, gut samples stained with Ziehl Neelsen, and blood samples from fish obtained and stained with Giemsa for analysis. The findings revealed that Trichodina sp. (85.71 percent) was present in several organs, with the gills being the most heavily affected organ. The intensity of this parasite was 27.83 individuals/head. The parasites detected by Ziehl Neelsen staining were Myxobolus sp. (57.14 %), Cryptosporidium spp. (35.71 %), and Cyclospora sp. (14.29%). The type of blood parasite found was identified as Hemogregarina sp (50%). Our findings are very important information for initiating the development of a treatment strategy to prevent parasite infection in tilapia fish farms.
The Quality of Indo-Pacific Bottlenose Dolphin (Tursiops aduncus) Sperm Following Liquid-storage in Low Temperature Fahrudin, Mokhamad; Gusdinar, Rizal; Arifiantini, Raden Iis; Prasetyaningtyas, Wahono Esthi; Adnyane, I Ketut Mudite; Elmanaviean, Muhammad; Nugraha, Arifin Budiman; Karja, Ni Wayan Kurniani
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 4 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.4.456-466

Abstract

Indo-Pacific bottlenose dolphin (Tursiops aduncus) is a marine mammal that lives in relatively small populations. The geographic ranges of this species are susceptible to the effects of human activities, thereby necessitating conservation efforts to prevent extinction. Therefore, this study aimed to evaluate the daily quality of dolphin sperm after several days of refrigeration. The sperm of two male dolphins were stored at 4oC for 4 days, and the quality was observed daily to determine the motility, viability, membrane integrity, and sperm abnormalities. Sperm samples were divided into four groups, consisting of two centrifuged followed by the removal of seminal plasma, and two groups without centrifugation, containing 100x106 and 200x106 sperm/ml each. After liquid storage, the motility of sperm was 63-75% with no significant reduction in the first 3 days. Sperm viability following storage was 65-75% and the percentage with abnormal morphology ranged from 2-6%. Furthermore, there was no significant increase in abnormal morphology of sperm on any day of storage for 3 days. Sperm membrane integrity was 36-49%, with no significant reduction in the membrane integrity in the first 2 days. There was no significant difference in sperm quality, although centrifugation and removal of seminal plasm had a slight effect. The results of this study showed that Indo-Pacific bottlenose dolphin sperm could be stored for a short period as liquid storage while maintaining a quality that allows for future use.
Molecular Identification of Babesia bigemina from Cattle and Buffaloes in Bogor District Qing Wen, Jyn Soong; Nugraha, Arifin Budiman; Latif, Hadri; Cahyaningsih, Umi
JURNAL KAJIAN VETERINER Vol 12 No 2 (2024): Jurnal Kajian Veteriner
Publisher : FAKULTAS KEDOKTERAN HEWAN UNIVERSITAS NUSA CENDANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35508/jkv.v12i2.17799

Abstract

Peternakan merupakan industri penting yang memberikan kontribusi signifikan terhadap sektor pertanian di Indonesia. Babesiosis adalah penyakit yang ditularkan melalui vektor caplak dan disebabkan oleh parasit darah genus Babesia. Penyakit ini telah menyebabkan kerugian ekonomi yang sangat signifikan. Deteksi molekular babesiosis memiliki sensitivitas dan spesifisitas yang tinggi. Namun, saat ini, deteksi babesiosis menggunakan Polymerase Chain Reaction (PCR) di Indonesia belum banyak digunaknan, khususnya di Kabupaten Bogor. Tujuan penelitian ini adalah untuk mengidentifikasi Babesia bigemina pada sapi dan kerbau di Kabupaten Bogor dengan teknik molekular. Sebanyak 27 sampel darah dikoleksi yang terdiri atas 22 sampel sapi dan 5 sampel darah kerbau. Sampel diwarnai dengan Giemsa 10%, selain itu sampel darah diekstraksi dan dilanjutkan dengan nested PCR. Hasil dari pemeriksaan apusan darah menunjukkan 81,48% positif terhadap Babesia spp, sedangkan hasil nested PCR 11,11% menunjukkan positif terhadap B. bigemina. Berdasarkan jenis hewan sapi dan kerbau positif terhadap B. bigemina, masing-masing sebesar 9,09% dan 20%. Hasil penelitian ini memberikan informasi dasar mengenai tingkat infeksi Babesia bigemina di kabupaten Bogor berdasarkan metode molekuler. Oleh karena itu, program pencegahan dan pengendalian terhadap infeksi parasit darah pada peternakan sapi dan kerbau sangat perlu dilakukan.
Comparison of Morphology and Protein Profile of Acetone and PBS-Fixed Toxoplasma gondii Tachyzoites for Detection of IgG and IgM Seropositivity VALINATA, SISCA; Cahyaningsih, Umi; Nugraha, Arifin Budiman; Kurniawati, Dyah Ayu; Desem, Muhammad Ibrahim; Fong, Sulinawati
Jurnal Sain Veteriner Vol 43, No 1 (2025): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.103155

Abstract

Toxoplasma gondii is an obligate intracellular protozoan that causes zoonotic diseases. The prevalence of toxoplasmosis in humans in Indonesia ranges from 9.7-70%. The parasite is difficult to detect in tissues; therefore, serological testing is the most common method for detecting antibodies against Toxoplasma. Antibodies are often used as the testing criteria for IgG and IgM antibodies. Previous research has shown that agglutination testing with acetone-fixed tachyzoites was only positive for acute infection. The aim of this study was to examine microscopic changes in acetone-fixed tachyzoites, determine their ability to detect IgG and IgM seropositivity, and detect specific proteins for IgG and IgM against toxoplasmosis. Tachyzoites were fixed with acetone (A) and PBS (P). After fixation, tachyzoites were prepared to observe the morphology and sonicated to obtain Soluble Toxoplasma Antigen (STA). STA was then subjected to SDS page and western blotting. The addition of aseton resulted in morphological and protein changes. Although changes occur, acetone-fixed tachyzoites can still react with IgG and IgM seropositivity. Protein bands that can be used as IgG seropositive markers in western blot testing are bands measuring 20, 24, 27, 73, and 110 kDa, and the combination of acetone fixation with anti-goat IgM conjugate will result in seropositive bands.
Pengembangan Deteksi Parasit Darah Theileria equi dan Babesia caballi secara Otomatis menggunakan Algoritma YOLOv8 Arif, Ridi; Nugraha, Arifin Budiman; Kedaton, Feni Gemala; Erlangga, Wishnu Kusumo Agung; Wibowo, Bagas Dwi Suryo
Jurnal Sain Veteriner Vol 43, No 2 (2025): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.104069

Abstract

Piroplasmosis pada kuda merupakan penyakit parasit darah yang disebabkan oleh Theileria equi dan Babesia caballi, menyebabkan anemia, ikterus, kegagalan organ, serta pembatasan aktivitas kuda, termasuk larangan mengikuti kompetisi. Deteksi piroplasmosis secara rutin masih mengandalkan pemeriksaan mikroskopis ulas darah untuk melihat morfometrik parasitnya sehingga membutuhkan keahlian tinggi dan memerlukan waktu relatif lama. Penelitian ini bertujuan mengembangkan sistem deteksi otomatis parasit T. equi dan B. caballi berbasis algoritma YOLOv8 untuk meningkatkan kecepatan dan akurasi identifikasi mikroskopis. Pengambilan data dilakukan dengan pembuatan preparat ulas darah dari kuda yang terdiagnosis positif piroplasmosis yang telah divalidasi oleh ahli dari Laboratorium Protozoologi SKHB IPB. Preparat diwarnai menggunakan giemsa 10%, dilakukan pengambilan gambar, pembuatan dataset, anotasi data, pelatihan model YOLOv8, serta pengujian performa sistem. Hasil penelitian menunjukkan performa model mendeteksi parasit dengan mAP50 sebesar 69,8%, mAP50-95 sebesar 40,5%, dan kecepatan deteksi 5,4 ms. Evaluasi performa manual menunjukkan akurasi 91%, presisi 98%, recall 92%, dan F1-score 95% dibandingkan pemeriksaan mikroskopis. Penelitian ini menyimpulkan bahwa sistem berbasis YOLOv8 mampu melakukan deteksi T. equi dan B. caballi secara otomatis dengan presisi tinggi dan waktu deteksi cepat sehingga berpotensi digunakan sebagai alat bantu diagnosis piroplasmosis yang lebih efisien.
EVALUATION OF B1 GENE TO DETECT Toxoplasma gondii: COMPARISON OF THREE SETS NESTED PCR PRIMER Ekawasti, Fitrine; Azmi, Zul; Subekti, Didik Tulus; desem, muhammad ibrahim; Nugraha, Arifin Budiman; Sadiah, Siti; Cahyaningsih, Umi
Jurnal Kedokteran Hewan Vol 17, No 2 (2023): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v17i2.22251

Abstract

This study aimed to evaluate three sets of B1 gene DNA primer for the diagnosis of Toxoplasma gondii. The DNA of Toxoplasma gondii that stored on liquid nitrogen was isolated using DNAzol reagent. The first step of Polymerase Chain Reaction (PCRs) was performed using external and internal primer sets, respectively, and then nPCR. PCR products sequencing was performed by Apical Science. All sequences were analysed using CLC Sequence Viewer Version 8.0 software and compared to sequence database that deposited in ToxoDB (Toxoplasma gondii genome database) using BLAST (https://toxodb.org/toxo/app). Each B1 gene primer was evaluated by performing single PCR (forward and reverse) and nested PCR reactions. Three sets of B1 gene primer have different amplification precision. According to the results of amplicon sequencing, the primer set #2 has the best amplification precision of B1 gene.
Co-Occurrence of Pseudopregnancy, Pyometra, and Bacterial Parasitic Anaplasmosis in a Persian Cat Budiono, Novericko Ginger; Rahmiati, Dwi Utari; Tuliman, Nurul Annisa; Delimasari, Zahrizada Zamrudina; Murtiningrum, Fitria Senja; Adistya, Erly Rizka; Nugraha, Arifin Budiman; Silvarajoo, Keertana; Milna, Resi
The International Journal of Tropical Veterinary and Biomedical Research Vol 8, No 2 (2023): Vol. 8 (2) November 2023
Publisher : The Faculty of Veterinary Medicine of Syiah Kuala University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/ijtvbr.v8i2.34156

Abstract

An intact female tabby Persian cat, 26-month-old, was brought to IPB University Veterinary Teaching Hospital on the 21st of February, 2023, due to vaginal discharge, inappetence, and lethargy. Physical examination revealed a distended abdomen, mammary gland enlargement, and fever (40.1 C). The heart and respiratory rates were 128 beats per minute and 32 times per minute, respectively. The patient body weight was 3.87 kg. The veterinarian palpated masses inside the abdominal cavity and observed obvious purulent and haemorrhagic vaginal discharge. Haematology showed leukopenia and thrombocytopenia, and the eosinophil percentage of white blood cells was increased (eosinophilia). Blood chemistry analysis showed hyperglycaemia. Ultrasonography showed two hyperechoic masses inside two hypoechoic pouches in the queens uterus, suspected as two dead foetuses (no heartbeat). Roentgen revealed abnormalities in the vena cava caudalis, bronchial pattern in the lung, radioopaque masses in the ventral abdomen, radio-opacity changes in the small and large intestines, and abnormalities in the spleen. The patients diagnosis was pyometra, and ovariohysterectomy surgery was performed on the patient. The patient was treated with additional medicines (antibiotics and others) according to the symptoms. The patients symptoms disappeared, except for the fluctuating febrile and pale mucous membrane. A blood smear performed on the 1st of March 2023 revealed Anaplasma phagocytophilum (A. phagocytophilum) in the white blood cells, and immediately doxycycline treatment (10 mg/kg body weight, twice daily) was given. The patient was discharged from the hospital on the 14th of March, 2023, with no febrile or other symptoms reported. This case is the first report of open cervix-type pyometra and anaplasmosis co-occurrence in a Persian cat from IPB University Veterinary Teaching Hospital in Indonesia.
The Presence and Infection Rate of Strongyloides sp. in Banteng (Bos javanicus) in Ujung Kulon National Park (UKNP) Handayani, Handayani; Agustin, Marini; Nugraha, Arifin Budiman
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Publisher : Universitas Pendidikan Indonesia and Konsorsium Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to identify and determine the level of Strongyloides sp. infection in buffalo (Bos javanicus javanicus) fecal samples in Ujung Kulon National Park (TNUK). The research method was conducted by examining fecal samples using native, sedimentation, and flotation methods and calculating the number of eggs per gram of feces (egg per gram, EPG) to determine the level of infection. The results showed positive Strongyloides sp. infection, indicated by the discovery of oval-shaped eggs with thin walls measuring approximately 54×41 µm. The EPG calculation yielded a value of 1600, which is categorized as a moderate infection. This level of infection indicates that the parasite's life cycle is active in the host's body and has the potential to cause mild to moderate physiological disturbances. Additionally, environmental conditions can increase the risk of reinfection, especially in wildlife habitats with high humidity levels. These findings emphasize the importance of continuous monitoring, environmental management, and parasite control measures, particularly in conservation areas such as Ujung Kulon National Park.
Identification of Endoparasites in Javan Banteng (Bos javanicus javanicus) in Ujung Kulon National Park (UKNP) Handayani; Marini Agustin; Arifin Budiman Nugraha; Tiara Primayanti
Al-Kauniyah: Jurnal Biologi Vol. 19 No. 2 (2026): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v19i2.46487

Abstract

Research on parasitic infections in the Javan rhinoceros (Rhinoceros sondaicus) and banteng (Bos javanicus javanicus) in Ujung Kulon National Park has been reported before. This study not only identifies the types of endoparasites but also measures the infection levels using eggs per gram of feces (EPG) and oocysts per gram of feces (OPG). This research aims to determine the impact of endoparasite infection on the health of the banteng (Bos javanicus javanicus) in its natural habitat at Ujung Kulon National Park (UKNP), through identifying the types of endoparasites that infect, prevalence analysis, and the level of infection. The sample identification was carried out at the Protozoology Laboratory of the Faculty of Veterinary Medicine and Biomedical Sciences of Institut Pertanian Bogor (IPB) University. Identification process using the flotation method, sedimentation method, native method, and the McMaster.The results of this research found five types of parasite eggs: Ascarid, Strongyloides, strongyloid, Paramphistomum, and Eimeria. The infection levels of each parasite, based on the calculation of eggs per gram of feces (EPG) and oocysts per gram (OPG), are as follows: ascarid at 1,050 EPG, Strongyloides at 1,600 EPG, Strongloides at 1,600 EPG, Paramphistomum at 50 EPG, and Eimeria at 550 OPG.
Prevalence and molecular characterization of Babesia and Theileria species in cattle from West Sumatra, Indonesia Umi Cahyaningsih; Yulia Yellita; Eliyus Putra; Roza; Fadjar Satrija; Ridi Arif; Dwi Inarsih; Budi Santosa; Arifin Budiman Nugraha
Jurnal Medik Veteriner Vol. 9 No. 1 (2026): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol9.iss1.2026.41-51

Abstract

This epidemiological investigation conducted in three regencies of West Sumatra, Indonesia, aimed to examine the molecular prevalence and distribution of Babesia bigemina, Babesia bovis, and Theileria orientalis. A cross-sectional study employing a convenience sampling technique included 151 cattle blood samples. These samples were collected from three regencies (Agam, Limapuluh Kota, and Tanah Datar) between January 2023 and February 2024. Blood samples were examined using polymerase chain reaction (PCR) coupled with sequencing, targeting the cytochrome b gene for B. bigemina and B. bovis, and the 18S rRNA gene for Theileria spp. A total of 151 cattle blood samples from West Sumatra were examined for hemoprotozoan infections using both microscopic and molecular approaches. Based on blood smear examination, 23 (15.2%) and 118 (78.1%) samples were positive for Babesia spp. and Theileria spp., respectively. Subsequent nested PCR analysis confirmed all Babesia-positive samples as B. bigemina, while B. bovis was not detected. In contrast, 131 (86.7%) samples were molecularly identified as T. orientalis. Phylogenetic analysis of the cytochrome b gene revealed three distinct clades of B. bigemina, indicating genetic diversity likely shaped by geographical distribution, vector ecology, or host adaptation. These findings demonstrate that T. orientalis is the predominant hemoprotozoan agent in West Sumatra, while the genetic variability of B. bigemina underscores the complexity of its epidemiology. Together, these results provide critical insights for improving surveillance and guiding the formulation of effective hemoparasite management strategies in the tropical Indonesian archipelago.
Co-Authors Abdul Zahid Adistya, Erly Rizka Afiff , Usamah Agus Wijaya Agustin, Marini Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Arni Diana Fitri Aviola, Lala Azizah, Hanifah Nur Azmi, Zul Budi Santosa Budiono, Novericko Ginger Chaerul Basri Delimasari, Zahrizada Zamrudina Denny Widaya Lukman Denny Widya Hikman Desem, Muhammad Ibrahim Didik Tulus Subekti Dwi Inarsih Dwi Utari Rahmiati Edi Sukmawinata Edi Sukmawinata Ekowati Handharyani Eliyus Putra Elmanaviean, Muhammad Erlangga, Wishnu Kusumo Agung Etih Sudarnika Fadjar Satrija Fajar Kawitan Fitrine Ekawasti, Fitrine Fong, Sulinawati Gusdinar, Rizal Hadri Latif Hammada Raudlowi Handayani Handayani Handayani Herwin Pisestyani Iccha Pradipta Patsiwi Jerica, Monica Silva Kedaton, Feni Gemala Ketut Adnyane Mudite Koekoeh Santoso Kurniawati, Dyah Ayu Mayasari, Ni Luh Putu Ika Milna, Resi Mirnawati B Sudarwanto1 Mirnawati Sudarwanto Mokhamad Fahrudin Muhammad Pauzi Lubis Murtiningrum, Fitria Senja Ni Wayan Kurniani Karja Nisrina Rosyida Noor Rifai Qing Wen, Jyn Soong R. Iis Arifiantini Rachmi Ramadhanita Ridi Arif Risa Tiuria Rita Kartika Sari Ronald Tarigan Rotinsulu, Dordia Anindita Roza Sadiah, Siti Silvarajoo, Keertana Siti Sa'diah Sri Murtini Subangkit, Mawar Suganti Veerasamy Tara Puri Ducha Rahmani Tiara Primayanti Tuliman, Nurul Annisa Ulfatin Khoiriyah Herowati Umi Cahyaningsih Valinata, Sisca Veenu Kumar Vetnizah Juniantito Wahono Esthi Prasetyaningtyas Wasrin Syafii Wibowo, Bagas Dwi Suryo Wirokartiko Satyawardana Yulia Yellita Yusuf Ridwan