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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Biospecies METAMORFOSA Journal of Biological Sciences Reaktor Bioma : Berkala Ilmiah Biologi Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Bahan Alam Terbarukan Biosfer: Jurnal Tadris Biologi Biogenesis: Jurnal Ilmiah Biologi Journal of Degraded and Mining Lands Management Indonesian Journal of Biotechnology Jurnal Agro Planta Tropika BIO-SITE |BIOLOGI Sains Terapan Majalah Obat Tradisional Journal of Natural Sciences and Mathematics Research Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology Omni-Akuatika Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Agrotek Tropika Jurnal Riset Biologi dan Aplikasinya Berkala Ilmiah Biologi International Journal of Oil Palm
Rina Sri Kasiamdari
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Education Energy Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Reaktor

 1 
CELL DISTRUPTION MIKROALGA SECARA ENZIMATIS DENGAN SELLULASE Padil Padil; Siti Syamsiah; Muslikhin Hidayat; Rina Sri Kasiamdari
Reaktor Volume 15 No.4 Oktober 2015
Publisher : Dept. of Chemical Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (551.658 KB) | DOI: 10.14710/reaktor.15.4.213-217

Abstract

ENZYMATIC CELL DISRUPTION OF MICROALGAE USING CELLULASE. Micro-algae is one source of potential alternative energy of third generation to be developed as bioethanol raw material. The starch content trapped in Micro-algae cell walls causing the need of cell distruption to release and convert starch into simple glucose before the fermentation process. This study aims to open up the cell walls of Micro-algae and to explore the effect of sellulase enzymes from Aspergillus niger as Micro-algae cell distruption strategy as well as the optimization of process parameters, i.e the concentration of enzyme, temperature, pH, and time which produce the highest glucose yield. The results showed that the highest glucose yield was 82.44% (w/w) obtained at an enzyme concentration of 30% (w/w), temperature 45oC, pH of 4.5 at 40 minutes, the amount of Micro-algae as 0.5 g/L. In general, cell distruption method using sellulase enzyme was proven to be a promising option to open the cell walls of Micro-algae and convert cellulose into simple glucose simultaneously in producing bioethanol.   Keywords: bioethanol; cellulose; cell distruption; enzymatic; micro-algae; starch Abstrak Mikroalga merupakan salah satu sumber energi alternatif generasi ketiga yang potensial untuk dikembangkan sebagai bahan baku bioetanol. Kandungan pati yang terperangkap dalam dinding sel mikroalga menyebabkan perlunya cell distruption untuk melepaskan sekaligus mengkonversi pati menjadi glukosa sederhana sebelum proses fermentasi. Penelitian ini bertujuan untuk membuka dinding sel mikroalga sekaligus mengeksplorasi pengaruh enzim sellulase dari aspergillus niger sebagai strategi cell distruption mikroalga serta melakukan optimasi parameter proses yaitu konsentrasi enzim, suhu, pH, dan waktu yang memberikan yield glukosa tertinggi. Hasil penelitian menunjukkan bahwa yield glukosa tertinggi adalah 82,44% (w/w) yang diperoleh pada konsentrasi enzim 30% (w/w), suhu 450C, pH 4,5 pada waktu 40 menit, dengan jumlah mikroalga 0,5 g/L. Secara umum, metode cell distruption dengan menggunakan enzim sellulase terbukti menjadi pilihan yang menjanjikan untuk membuka dinding sel mikroalga sekaligus mengkonversi selulosa menjadi glukosa sederhana dalam memproduksi bioetanol. 
Co-Authors ', Padil AA Sudharmawan, AA Achmadi Priyatmojo Adiana Nayogyani Ali Djamhuri Ali, Nur Ayu Aisyah Ana Susianti Ani Widiastuti, Ani Auli, Nisa Raudatul Ayundai, Melin Ayundai, Melin BUDI SETIADI DARYONO BUDI SETIADI DARYONO C. J. Soegihardjo C.J. Soegihardjo C.J. Soegihardjo C.J. SOEGIHARDJO Chasanah, Laila Uswatun Dhia Salsabila Hakim Dian Fitriarni Ekowati , Nuraeni Elzahra Nadya Putri Endang Sutariningsih Soetarto ENDANG SUTARININGSIH SOETARTO Erni Martani Erni Martani Febriani, Anya Via Ganies Riza Aristya Hasnaul Maritsa I Gusti Wayan Murjana Yasa Inayati, Evi Indra Sukmawati Junita Hardini Khaterine K Kristamtini Kristamtini, Kristamtini Kusrinah Kusrinah Kusrinah Kusrinah Laila Hanum Maulin Nafisa Muazam, Arif Muslikhin Hidayat Nita Aminasih Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo Nursamsi Pusposendjojo, Nursamsi Nursela, Dewi Padil, Padil Purnomo Purnomo Pusposendjojo , Nursamsi Putranto, Dwiyandito Ikhsan Putri, Elzahra Nadya Putri, Fauzana Putri, Fauzana R. Rugayah Ratna Stia Dewi Ratna Stia Dewi Reine Suci Wulandari Rejeki Siti Ferniah Retno Peni Sancayaningsih Retno Peni Sancayaningsih RETNO PENI SANCAYANINGSIH Ristiyani Khofifa Putri ROSA SURYANTINI Rugayah Rugayah S. Santosa Santosa . Santosa Santosa Santosa Santosa Sari, Noorkomala Setyorini Widyayanti, Setyorini Singgih Tri Wardana Siti Syamsiah Siti Syamsiah Soegihardjo , C.J. Soekarti Moeljopawiro Soekarti Moeljopawiro, Soekarti Suharno Suharno Susiana Purwantisari Sutikno S Syaifudin, Andang Syamsiah, Siti Widiastuti, Ani Yekti Asih Purwestri Yudhistira Nugraha