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Journal : Jurnal Laboratorium Khatulistiwa

Analisis Kadar Asam Asetat (CH3COOH) Dalam Cuka Rambai Yang Difermentasi Pada Industri Rumahan Di Desa Sungai Pangkalan Kecamatan Sungai Raya Triana, Linda; Sari, Emilda
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1669

Abstract

Rambai fruit (Baccaurea motleyana) is a fruit that is still unable to compete with other local fruits, so it has lower economic value. Not only is it eaten directly, but rambai fruit can also be processed into vinegar. Vinegar is a substance made from various sugary or starchy ingredients through alcoholic fermentation followed by acetic fermentation. The vinegar sold must contain at least 4% (4 grams of acetic acid per 100 ml), must be fresh, and be made from fruit that is suitable for consumption. The vinegar that is widely available on the market and consumed by the public is made from chemicals that are harmful to the body. For this reason, it is necessary to make natural innovations in making vinegar, for example by using fruit. This research aims to determine the levels of acetic acid (CH3COOH) in rambai vinegar fermented for 1, 3, 12, and 36 months. The research method uses a descriptive research design. The sample used was rambai vinegar produced from a home industry in Sungai Pangkalan Village, Sungai Raya District, fermented for 1, 3, 12 and 36 months. Titrimetric examination method. Research on acetic acid levels in rambia fruit vinegar fermented for 1, 3, 12, and 36 months found 1.5802%, 1.5848%, 1.5917%, and 1.6991%. The conclusion was that the acetic acid content in Zambia fruit vinegar fermented for 1, 3, 12, and 36 days was still below the Indonesian national standard, namely 4-12%.
UJI EFEKTIVITAS KOMBINASI PERASAN BUAH LABU AIR DAN JAHE MERAH SEBAGAI ANTIBAKTERI Salmonella typhi Djohan, Herlinda; Triana, Linda; Nurhayati, Etiek
Jurnal Laboratorium Khatulistiwa Vol 8, No 1 (2024): November 2024
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i1.1584

Abstract

Typhoid fever is an acute disease caused by the bacteria Salmonella typhi. This bacteria is usually found in contaminated water or food. Apart from that, this bacteria can also be transmitted from infected people. The treatment carried out is using modern drugs such as the antibiotic chloramphenicol. However, the use of modern medicine has several disadvantages such as side effects. On the other hand, the use of natural ingredients as natural medicine has been widely used. One of them is that water gourd is empirically used as a fever reducer. Water gourd contains secondary metabolites, namely saponins, steroids and phenols which have been proven to have antibacterial activity and are able to inhibit the growth of Salmonella typhi bacteria. Another natural ingredient that has antibacterial activity is red ginger. Red ginger contains active compounds that have antibacterial effects such as flavonoids, phenols, treponoids and essential oils. Antibacterial activity was obtained by measuring the diameter of the inhibition zone after administering 4 variations of the concentration of the juice sample. The positive control used amoxicillin 30 µg/disk and the negative control used distilled water. The research design used was a quasi-experimental method. The samples were water gourd and red ginger juice with varying concentrations of 40%, 60%, 80% and 100% and the average inhibition zone values were obtained, namely 8.875 mm, 9.875 mm, 11.25 mm and 14.25 mm. Based on the results of research and computerized data processing using the Kruskall Walis test, a value of p = 0.000 (p<0.05) was obtained, which means that there is an effect of the concentration of pumpkin juice and red ginger on the inhibitory power of Salmonella thypi bacteria.
The Effect of Kratom Leaf Alkaloid Extract on Blood Glucose Levels Using an In Vivo Method Dewi, Bastiana; Kamilla, Laila; Sutriswanto, Sutriswanto; Tumpuk, Sri; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1851

Abstract

Kratom contains alkaloids, flavonoids, saponins, and tannins, which have traditionally been used to boost energy and treat various health conditions, including diabetes. This study aims to analyze the effect of kratom leaf alkaloid extract on blood glucose levels in vivo using a quasi-experimental design. The subjects were 27 male Swiss Webster mice (Mus musculus), divided into three treatment groups receiving kratom leaf alkaloid extract at doses of 0.147 mg/20gBW, 0.294 mg/20gBW, and 0.588 mg/20gBW. The alkaloid extract was obtained through fractionation. Each treatment group was replicated nine times using purposive sampling. An oral glucose tolerance test was conducted to measure blood glucose levels. The Simple Linear Regression test results showed a p-value of 0.000 (p < 0.05), indicating a significant effect of kratom leaf alkaloid extract on blood glucose levels in vivo.
Kontak Serumah dan Kejadian Penularan Tuberkulosis di Pontianak Barat Nurhayati, Etiek; Rahmawati, Eri; Sutriswanto, Sutriswanto; Tumpuk, Sri; Triana, Linda
Jurnal Laboratorium Khatulistiwa Vol 8, No 2 (2025): Mei 2025
Publisher : poltekkes kemenkes pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30602/jlk.v8i2.1859

Abstract

Tuberkulosis adalah penyakit menular disebabkan oleh Mycobacterium tuberculosis. Penyakit Tuberkolosis paru ditularkan melalui udara (droplet nuclei), saat penderita batuk, bersin atau berbicara, kuman TB paru yang berbentuk droplet akan bertebaran di udara. Kontak serumah dengan penderita tuberkulosis aktif berisiko tertular dibandingkan dengan yang tidak kontak dengan penderita tuberkulosis paru. Penelitian ini bertujuan untuk mengetahui risiko penularan TB paru pada orang yang kontak serumah meliputi lama kontak dan tidur sekamar dengan penderita di wilayah kerja Puskesmas Perumnas II Kecamatan Pontianak Barat. Metode penelitian ini adalah deskriptif analitik dengan menggunakan teknik pengambilan sampel yaitu total sampling.  Jumlah sampel sebanyak 77 responden yang tinggal serumah dengan penderita tuberkulosis paru yang masih aktif berobat di wilayah kerja Puskesmas Perumnas II Kecamatan Pontianak Barat, 2024. Pengambilan data dilakukan dengan wawancara, kunjungan rumah dan pemeriksaan laboratorium BTA secara mikroskopis. Hasil analisis menunjukkan dari 77 responden yang memiliki kontak dengan penderita ≥ 8 jam sebanyak 37 responden (48,1%) dan didapatkan 4 responden yang hasil BTA (+). Lama kontak dengan prevalensi sebanyak (10,8%) dan tidur sekamar dengan prevalensi sebanyak (36,3%). Maka disimpulkan bahwa terdapat  penularan TB Paru pada orang yang kontak serumah dengan penderita di Wilayah Kerja Puskesmas Perumnas II Kecamatan Pontianak Barat.