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Journal : Proceeding Biology Education Conference

POTENSI GENISTEIN PADA SISTEM REPRODUKSI MENCIT JANTAN (Mus musculus) Cicilia Novi Primiani; Umie Lestari; Mohamad Amin
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 8, No 1 (2011): Prosiding Seminar Nasional VIII Biologi
Publisher : Universitas Sebelas Maret

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Abstract

ABSTRAK Usaha untuk mendapatkan sarana kontrasepsi pria sebenarnya telah banyak dilakukan dengan memanfaatkan bahan alami yang berasal dari tanaman. Salah satunya adalah tanaman famili Leguminoceae yang mengandung senyawa genistein. Genistein sebagai salah satu senyawa derivat isoflavon mempuyai struktur kimia mirip dengan 17β-estradiol yang bersifat seperti hormon steroid estrogen, yang mampu menyebabkan kerusakan pada sistem reproduksi jantan sebagai salah satu indikator digunakannya genistein sebagai senyawa antifertilitas. Penelitian ini bertujuan untuk mengetahui pengaruh genistein terhadap sistem reproduksi mencit jantan (Mus musculus). Penelitian ini menggunakan pendekatan kuantitatif eksperimental, yang rancangannya mengikuti Rancangan Acak Kelompok. Variabel bebas adalah dosis genistein 0 mg/g, 0,0035 mg/g, 0,0042 mg/g, dan 0,0049 mg/g. Variabel terikatnya adalah sel-sel germinal dalam tubulus seminiferus testis, morfologi spermatozoa, viabilitas spermatozoa, dan kadar hormon testosteron. Data sel-sel germinal dalam tubulus seminiferus testis kadar hormon testosteron dianalisis menggunakan Analisis Varians Satu Jalan (One Way ANOVA) dengan tingkat signifikansi 5%. Selanjutnya dilakukan uji Post Hoc dengan Least Significant Difference (LSD) 5%. Data morfologi dan viabilitas spermatozoa dinyatakan dalam prosentase. Hasil penelitian menunjukkan bahwa ada pengaruh genistein pada sistem reproduksi mencit jantan (Mus musculus). Genistein yang diberikan dengan dosis 0,0035 mg/g; 0,0042 mg/g; dan 0,0049 mg/g berpengaruh terhadap jumlah sel germinal, morfologi dan viabilitas spermatozoa. Kadar hormon testosteron berbeda nyata pada dosis 0,0049 mg/g. Kata kunci : genistein, sistem reproduksi jantan
IDENTIFIKASI KERAGAMAN GENETIK KERBAU LOKAL POPULASI JAWA TIMUR DAN NUSA TENGGARA BARAT BERBASIS MIKROSATELIT SEBAGAI MODEL PENGEMBANGAN KONSERVASI KERBAU SECARA EX SITU Mohamad Amin; Umie Lestari
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 11, No 1 (2014): Prosiding Seminar Nasional XI Biologi
Publisher : Universitas Sebelas Maret

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Abstract

In Indonesia, local buffalo (Bubalus bubalis) is one type of germplasm that a major contribution in the national meat supply of animal protein. However, this germplasm will be reduced of its number.  If this condition continues we will loss the germplasm . One effort to this is conservatuin based on  a molecular marker mikrosatellite . Selected marker locus are HEL09 , INRA23 and ISLTS05 . Sampling area of it research in East Java and West Nusa Tenggara . The results showed that in both are and all loci is polymorphic, allele frequencies ranging from 0.125 to 0.875 and heterozygosity values in the range 35-65 % . These results illustrate that the two populations have genetic diversity that is still quite varied genotype . Key Words: alele frequency, heterozigosity, local buffalo, microsatellite
Students’ Responses and Expectations about the Opportunity of Virtual Laboratory Using in Genetics Courses Dewi Murni; Mohamad Amin; Umie Lestari; Sri Rahayu Lestari; Sri Endah Indriwati
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 17, No 1 (2020): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

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Abstract

This study was conducted to describe students’ perceptions about the genetic course, laboratory experiment, laboratory facilities, and also students’expectations about the development of the virtual laboratory as an alternative experiment in Genetics Courses. A descriptive quantitative method was used in this study. The participants involved were 49 students enrolling in a genetics course at a public university in Banten, Indonesia. Data were collected through a set of questionnaires. To analyze the data,we employed a descriptive quantitative approach in the form of a percentage. The results showed that the genetic course was considered difficult by the students. The reason is that the material is abstract, and the source of learning is difficult to understand. The most commonly used learning media in the lecture is Power Point.Learning strategies are oftenused,i.e.,the discussions and laboratory work in the laboratory. Students need interesting, communicative, and interactive learning media. The student response is enthusiastic about virtual laboratory development. The expected virtual laboratory criteria are can be used offline, looks interesting, easy to operate, and containsaudio-visual.
Co-Authors Abda Abda Abdul Gofur Abdul Gofur Abdul Gofur Abdul Gofur Abu Husen Abu Husen Afifah Nur Aini Alif Yanuar Zukmadini Aloysius Duran Corebima Amin, Ihya Fakhrurizal Anton Setia Budi Ardiyas Robi Saputra Basuki B Purnomo, Basuki B Beno Wahono Beno Wahono, Beno Betty Lukiati Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani Cicilia Novi Primiani, Cicilia Desy Putri Rahmawati Dewi Murni Dina Maulina Dwi Anggorowati Rahayu Endang Suarsini Endik Deni Nugroho Fahmi, Muhammad Najib Farizha Irmawati Farizha Irmawati, Farizha Fitri, Nurul Hadi Suwono Haqqi Anajili Setyanto Haqqi Anajili Setyanto, Haqqi Anajili Herawati Susilo Ihya Fakhrurizal Amin Ika Oktaviana Ika Oktaviana, Ika Istamar Syamsuri, Istamar Istamar Syamsyuri Izza Rohman Kurniawan Setia Putra Listawati, Rizki Mei Misgiati, Misgiati Mochammad Ali Mashur Moh. Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohamad Amin Mohammad Amin Mohammad Amin Mohammad Amin Monalisa PJ Taihuttu Muh. Nur Akbar Murni Sapta Sari Nafisatuzzamrudah Nafisatuzzamrudah Nanda Earlia Nandang Mufti Naning Wahyuni Nindya Ulfa Wardhani Nugrahaningsih Nugrahaningsih Nur Hayati Rahman Fadli Rahmawati D Rahmawati Darussyamsu Reni Istiningrum Reni Istiningrum, Reni Ridho, Muhammad Andi Ali Ristiana, Evi Ritia Rahmawati Ritia Rahmawati, Ritia Rizka Elan Fadilah, Rizka Elan Rizki Mei Listawati Rizkia Suciati Rizkia Suciati Saparuddin Saparuddin Sembiring, Rinawati Siti Nur Arifah Siti Rahma Lestaluhu Sri Endah Indriwati Sri Rahayu Lestari Sulistyo Dwi Kartining Putro Sulistyo Dwi Kartining Putro, Sulistyo Dwi Kartining Susriyati Mahanal Sutiman B Sumitro Sutiman B Sumitro, Sutiman Sutiman B. Sumitro Ulin Nuha Ulin Nuha, Ulin Ulya Ruwaida Umi Fitriyati Urla Tri Wulanzani Urla Tri Wulanzani, Urla Tri