Yudiwanti Wahyu E. Kusumo
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor (Bogor Agricultural Universtity), Jl Meranti, Kampus IPB Darmaga, Bogor 16680, Indonesia

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Journal : Journal of Tropical Crop Science

Evaluation of Leaf Spot Resistance and Agronomic Characteristics of Groundnut Advanced Breeding Lines Wahyu, Yudiwanti; Achmad, Achmad; Junaedi, Wahyu
Journal of Tropical Crop Science Vol 3 No 3 (2016): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.984 KB) | DOI: 10.29244/jtcs.3.3.82-88

Abstract

Leaf spot is a major groundnut disease in Indonesia and worldwidewhich has the potential to cause a yield loss of up to 60%. The use of leaf spot resistant cultivars is an easy, economical, and environmentally friendly way in groundnut cultivation to maintain high yields. The objective of the research was to evaluate the yields of 16 groundnut lines which had been developed to be leaf spot resistant and high yielding. The research was conducted in 2010 – 2011 at the Bogor Agricultural University (IPB) experimental station in Cikarawang, Indonesia. A randomized complete block design with three replications was used in the experiment. The groundnut lines evaluated were crosses between “Gajah”, a cultivar which is known to be susceptible to leaf spot, and the resistant line GPNC- WS4. Four Indonesian cultivars “Gajah”, “Jerapah”, “Zebra” and “Sima” were used as control. The results showed that selected lines demonstrated better resistance to leaf spot compared to “Gajah”. Their agronomic characteristics, such as pod number, filled pod number, pod weight, and kernel weight, were not significantly different from the control cultivars. The results of this study have provided information on groundnut leaf spot resistant lines for further evaluation.
Determination of the Optimum Rate of N Fertilizers with Addition of Goat Manure for Production of Cowpea (Vigna unguiculata [L.] Walp) Liana, Devi; Purnamawati, Heni; Melati, Maya; Wahyu, Yudiwanti
Journal of Tropical Crop Science Vol 6 No 02 (2019): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (806.186 KB) | DOI: 10.29244/jtcs.6.02.121-128

Abstract

Cowpea (Vigna unguiculata L. Walp) is one of the potential substitutes of soybean due to its similar nutritional content. This study aims to determine the optimum rate of N fertilizer for the production of cowpea, and to determine the effects of the interaction between N fertilizer rates and goat manure application on cowpea production. The experiment was organized in a split-plot with a complete randomized block design with three replications. The main plot was goat manure, i.e. 0 and 5 tons.ha-1; the sub-plot was nitrogen fertilizer with five rates (0, 50, 100, 150, and 200% of the recommended rate), i.e. 0, 22.5, 45, 67.5, and 90 kg N.ha-1. Application of N fertilizer reduced the 100-seed weight and slightly reduced cowpea yield and yield components. Application of goat manure increased seed dry weight per plant, number of pods per plant, dry pod weight per plant, seed dry weight per m2, productivity, 100-seed weight, and the harvest index. There was no significant interaction between goat manure and different rates of nitrogen in affecting cowpea growth.
Advanced Yield Trial for Various Peanut Lines (Arachis hypogaea L.) at Sodonghilir, Tasikmalaya, West Java, Indonesia Siti Nurhalimah; Yudiwanti Wahyu; Siti Nurhidayah; Efrin Firmansyah
Journal of Tropical Crop Science Vol. 8 No. 01 (2021): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.8.01.22-32

Abstract

This study aims to evaluate yield and yield components in several peanut lines planted in West Java, Indonesia. The genetic materials tested involved 21 genotypes consisting of 18 potential peanut lines (G100, G133, G142, G144, G199, G205, G209, G21, G234, G237, G33, G37, G41, G53, G54, G76, G84, G99) from a selection of 5 biparental population (GWS79A1/”Zebra”, “Jerapah”/GWS79A1, “Zebra”/GWS79A1, GWS79A1/”Jerapah”, “Zebra”/GWS18A1) and 3 comparative varieties (“Gajah”, “Sima”, and “Zebra”) as controls. Results showed that various genotypes have significant effects on plant height (at 9 and 10 WAP), number of branches (at 4, 10, and 12 WAP), flowering age, and harvest age. Some potential lines showed ideal characters expected of a high quality peanut plant. Based on characters such as harvest age, dry pod weight, and productivity, G100, G41, G21, G205, and G84 can be considered the best potential lines. These lines demonstrated high heritability values including characters classified as high category such as plant height, number of branches, age of flowering, age of harvest, harvest index, wet stover weight, fresh pod weight, number of filled pods, number of empty pods, number of filled pods, and weight of 100 seeds. Additionally, these lines also show high productivity, a character classified in the medium category.
Agronomic Performance of IPB’s Red Chili Pepper (Capsicum annuum L.) Lines in Different Environments in Bogor, West Java, Indonesia Tobias Moniz Vicente; Yudiwanti Wahyu; Muhamad Syukur; Asep Setiawan
Journal of Tropical Crop Science Vol. 9 No. 03 (2022): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.9.03.214-222

Abstract

Our study aims to determine the agronomic performances of the red chili pepper genotypes developed by the IPB University as compared to the existing commercial varieties. The study was conducted from June 2019 to April 2020 in the Leuwikopo and Tajur experimental fields, IPB University, Bogor, West Java, Indonesia. Leuwikopo and Tajur location differs in the soil type and environments. The experiments at Leuwikopo were conducted twice, once during the dry season, and once during the rainy season. The experiments were set up in a completely randomized block design with a single factor, i.e. sixteen red chili pepper lines, replicated three times in three different environments. The 11 lines evaluated were F8120005-141-16-35-1-3, F8120005-141-16-35-1-4, F8120005-141-16-35-7-1, F8120005-241-2-9-4- 4, F7120005-120-7-1-7-8-1-2, F6074-7-4-2-1, F4074136-2-3, F474077-1, F474035-2-1, F474035-2, F4136074-1-4; these lines were compared to five commercial chili varieties, “Laris”, “Gada” F1, “Kastilo” F1, “Anies IPB”, “Seloka IPB”. Measurements were conducted on time to harvest, fruit length, fruit diameter, weight per fruit, number of fruits per plant, fruit weight per plant, and productivity. The results showed that the curly and large red chili pepper genotypes had similar agronomic performances to the control curly variety “Laris” and “Kastilo” F1, and and “Anies IPB”, “Seloka IPB”, or “Gada” F1 as control variety for the large chili. Our study demonstrated that all large and curly red chili pepper lines developed in IPB University have excellent potentials to be commercialized into superior varieties.
Performance of Convergent Breeding Wheat (Triticum aestivum L.) Lines in the Lowlands Habibi Firmansah; Yudiwanti Wahyu; Amin Nur
Journal of Tropical Crop Science Vol. 11 No. 01 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.01.9-18

Abstract

The development of tropical wheat in Indonesia is currently confined to the availability of wheat’s optimal environments in the highlands. Wheat competes with major highland crops, such as vegetables, which also have high economic values. Despite this, the demand for wheat in Indonesia remains high, whether in the form of wheat flour, wheat meal, or oats. Wheat breeders are actively working to create various crossbreeds so that wheat can adapt and perform effectively in lowland areas. The convergent breeding method is one of the strategies employed to produce genotypes with superior performance. Convergent breeding enhances genetic diversity by incorporating superior traits from all parent plants. The breeding results expedite the emergence of genetic combinations between selected parents. This method involves combining several parent varieties with various traits, with the hope that their offspring will inherit all the characteristics of the crossed parents. Our study with wheat convergent breeding has reached the F6 generation, and in this current study we evaluated the performance of each observed trait in different environments, with the goal of determining the levels of homogeneity and homozygosity. The study utilized a randomized complete block design with three replications, and the crops were planted in various locations. The planting locations selected were those that are >1000 m above sea level (asl), and at a lowland of ± 250 m asl. Wheat performance based on stomatal characteristics showed a reduction in the lowland, which indicates a response to climatic conditions in a particular environment. The higher the environmental temperatures, the smaller the stomatal size, which reduces plant water loss. Noteworthy findings include the tallest plant in CBF-6. CAMN23(265), the highest number of tillers in CBF-6. CAMN233 and CBF-6.CAMN8(4), the largest flag leaf area in CBF-7.CAMN60, and the highest 100-seed weight, as well as overall yield in CBF-7.CAMN119. An analysis of the lowland sensitivity index identified ten moderate genotypes that could potentially adapt well and achieve optimal yields.
Variability of Agronomic Traits of Wheat (Triticum aestivum L.) Lines in Two Agroecosystems in Indonesia Herdhani, Mira; Wahyu, Yudiwanti; Trikoesoemaningtyas
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.229-306

Abstract

Wheat is an important agricultural commodity in Indonesia, yet the country remains heavily reliant on imports to meet domestic demand. The diverse agroclimatic conditions in the highlands and lowlands pose significant challenges for national wheat production. This study aimed to evaluate the performance of wheat lines in two distinct altitude environments: Cipanas (1120 m above sea level) and Cisarua (600 m above sea level).  An augmented randomized complete block design (RCBD) assessed 50 F6 wheat lines derived from the HP1744/”Selayar” cross. The results revealed significant variability in agronomic traits attributable to altitude differences. However, genotype-by-environment (GxE) interactions were found to be non-significant for all evaluated characters. Broad-sense heritability was classified as medium for the grain-filling period in Cipanas and seed weight of the main panicle in Cisarua, while all other traits exhibited low heritability. Based on the stress sensitivity index (SSI), 14 lines (28%) were identified as tolerant to higher temperatures in the medium-altitude environment, particularly in terms of seed weight of the main panicle. These findings are of utmost importance as they underscore the need to consider genetic and environmental factors in developing wheat cultivars suitable for Indonesia’s diverse agro-climatic conditions.
Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Tihurua, Eka Fatmawati; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.307-319

Abstract

Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis, from induction, culture proliferation, and somatic embryo development to plantlet conversion. The initial stages of embryogenesis, characterized by nodules, were primarily composed of meristematic cells with high cell division activity. These cells contained tetrad-like structures that could develop into distinct two- and four-celled proembryoids or proembryogenic aggregates. Our histo-anatomical analysis revealed that embryogenic cultures proliferated through multiple pathways simultaneously: somatic embryo budding, proembryo formation, and pro-embryonic mass formation from both internal and peripheral cells. At the stage of somatic embryo development, embryos with a well-defined protoderm layer, containing cells with prominent nuclei and dense cytoplasm, potentially regenerate into plantlets. Furthermore, histological examination revealed the presence of procambium within mature somatic embryos, which subsequently developed into the vascular system of the complete plantlet
An Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.172-184

Abstract

Wild banana Musa acuminata ssp. malaccensis, an ancestor of cultivated bananas, possesses valuable genetic diversity, including resistance genes to fusarium wilt, and demonstrates high environmental adaptability. These traits are important for pre-breeding programs, whether by conventional breeding, which is a lengthy process, or by taking advantage of somatic cell manipulation techniques such as somatic hybridization, which requires an efficient plant regeneration system like somatic embryogenesis. We have established an efficient and comprehensive protocol for somatic embryogenesis of this wild Musa using immature zygotic embryo explants covering culture induction and proliferation, somatic embryo development, and subsequent plant conversion. Embryogenic culture was induced on a complex-modified MS medium supplemented with 1 mg.L-1 2,4-D auxin or 5 mg.L-1 picloram. The embryogenic cultures proliferated in the form of granular or nodular structures, which was best obtained by reducing the picloram concentration to 1 mg.L-1 and combining it with the same concentration of 2,4-D at a half-strength macro-nutrient of basal medium. Embryo development from embryogenic cultures and regeneration into shoots. Proembryos as granular structures spontaneously matured into early-stage somatic embryos upon withdrawal of the strong auxin inducer. Increasing the sucrose and gelling agent concentrations in the growth medium improved somatic embryo formation from embryogenic cultures. The frequency of shoot formation from developed somatic embryos was increased by incorporating 0.5 mg.L-1 BA and 0.5 mg.L-1 GA3 into the regeneration medium.
Co-Authors , Rosita Abdul Hakim Abdul Hakim Abdul Qadir Achmad Achmad Aditya Permana Samosir Aditya Permana Samosir Ahmad Ansori Mattjik Ahmad Ansori Mattjik Ahmad Ansori Mattjik Ajmilatunnisa Amin Nur Amin Nur Ani Kurniawati Arifah Rahayu Awang Maharijaya Baiq Arriyadul Badi'ah Cut Tia Mardi dan Nurita Toruan-Mathius Dede Rosyana Budiman Dede Rosyana Budiman Desta Wirnas Dhiea Falihatusy Syarifah Diyah Martanti, Diyah Dwi Yono Edison Jambormias Efrin Firmansyah Eka Bobby Febrianto, Eka Bobby Eka Fatmawati Tihurua Ekasari Putri, Nurwanita Eko Sulistyono Eni Sumarni Erin Puspita Rini Erniati Erniati Eti Rohaeti Fani Fauziah Faqih Udin fatimah Fatimah Folkes Laumal H. M.H. Bintoro Djoefrie H.M.H. BINTORO DJOEFRIE Habibi Firmansah Herdhani, Mira Herry Suhardiyanto Ika Roostika Indriani Nurul Fitri Ireng Darwati Irmanida Batubara Junaedi, Wahyu Jundi Aiman Abdullah Kurniawan, Rizal Mahdi Liana, Devi Linda Novita Mahat Magandhi Mahrup Mahrup, Mahrup Matra, Deden Derajat Mattjik, Ahmad A Maya Melati Memen Surahman Mohamad Solahudin Muhamad Syukur Muhammad Ridha Alfarabi Istiqlal Muhammad Syukur Muhammad Syukur Muhammad Yusuf Pulungan Nadirman Haska Nailan Nabila NFN Sudarsono Nisa, Febianti Khoirun Nugroho, Sasmoyo Adi Nurul Afiyah Otih Rostiana Patty, Jacob R Prawestri, Apriliana Dyah Rizal Mahdi Kurniawan Safitri Nuraina Safitri, Vika Ayu Sasmoyo Adi Nugroho Setiawan, Asep S Shadila Fira Asoka Shalati Febjislami Sintho Wahyuning Ardie Siti Hapshoh Siti Marwiyah Siti Nurhalimah Siti Nurhidayah Slamet Widodo Sobir , Sobir Sobir Sofyan Zaman Sri Gajatri Budiarti Sri Gajatri Budiarti Sri Soenarsih DAS Sri Wardani Sudarmono Sudarsono SUDARSONO SUDARSONO Supriyanto Supriyanto Surjono H Sutjahjo, Surjono H Surjono Hadi Sutjahjo Susi Purwiyanti Suwarno, Punjung Medaraji Tobias Moniz Vicente Tri Handayani Trikoesoemaningtyas Trikoesoemaningtyas Undang Undang Widodo Widodo Willy Bayuardi Suwarno Witjaksono Yuliawati