Claim Missing Document
Check
Articles

Found 38 Documents
Search

ANALISIS BAHAN KIMIA OBAT DEKSAMETASON DALAM JAMU PEGAL LINU MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI Aqnes Budiarti; Muhamad Barik Ulfa Faza
CENDEKIA EKSAKTA Vol 3, No 1 (2018)
Publisher : Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.3194/ce.v3i1.2136

Abstract

Deksametason merupakan bahan kimia obat yang seringkali ditambahkan secara ilegal pada obat tradisional pegal linu sehingga dapat membahayakan kesehatan. Dengan demikian perlu dilakukan analisis penetapan kadar bahan kimia obat dalam obat tradisional menggunakan metode yang akurat, tepat dan sensitif untuk memastikan keamanan obat tradisional. Tujuan penelitian ini adalah melakukan validasi metode penetapan kadar deksametason menggunakan kromatografi cair kinerja tinggi (KCKT) dan mengaplikasikannya pada obat tradisional pegal linu  yang ditambahkan deksametason. Penetapan kadar deksametason menggunakan seperangkat alat KCKT (Jazco) yang dilengkapi dengan detektor UV-Vis. Fase diam yang digunakan yaitu C18 serta fase gerak berupa campuran 0,1% asam ortofosfat dan asetonitril dengan laju alir 1 mL/menit. Uji validasi yang dilakukan meliputi uji presisi, akurasi, selektivitas (spesifisitas), linieritas dan sensitivitas (LOD dan LOQ). Hasil penelitian menunjukkan bahwa metode penetapan kadar deksametason dapat divalidasi. Uji presisi menghasilkan ketelitian (%RSD) =0,32%, uji akurasi memberikan nilai perolehan kembali sebesar 100,19%- 100,80%, selektivitas baik, linieritas dengan nilai korelasinya (r)= 0,999. LOD sebesar 0,93μg/mL dan LOQ sebesar 3,09 µg/mL. Kadar rata-rata deksametason dalam obat tradisional pegal linu adalah 100,18%. Kata kunci : deksametason, jamu pegal linu, KCKT
PENGARUH VARIASI KONSENTRASI TWEEN 80 dan SPAN 80 TERHADAP KARAKTERISTIK KRIM EKSTRAK ETANOL DAUN NANGKA (Artocarpus heterophyllus) SEBAGAI ANTIOKSIDAN Emy Susanti; Aqnes Budiarti; Maul Musyafaah
CENDEKIA EKSAKTA Vol 4, No 2 (2019)
Publisher : Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.3194/ce.v4i2.3052

Abstract

ABSTRACT Jackfruit leaves (Artocarpus heterophyllus) are known to have antioxidant activity and formulated in cream. Span 80 and tween 80 are emulsifiers that are widely used in cream formulations. This study aims to determine the antioxidant activity in cream preparations and the effect of concentrations span 80 and tween 80 on the physical characteristics of the cream.The ethanol extract of jackfruit leaves was made by maceration using 70% ethanol. Jackfruit ethanol extract cream is made in 4 formulas based on variations in the concentration of emulgator span 80; tween 80 that is F1 (3%; 3%), F2 (4%; 4%) and F3 (5%; 5%). The cream tested the physical characteristics of the cream including organoleptic, homogeneity, pH, adhesion, spreadability and viscosity, while antioxidant activity carried out by DPPH (1,1-Diphenyl-2-picrylhidrazyl) method. Data on the physical characteristics of the cream obtained were analyzed using linear regression, the results of organoleptic test and homogeneity were analyzed descriptively, and data from the activity of antioxidant creams were statistically tested using One Way Anova. The results obtained showed variation in concentrations of span 80 and tween 80 affecting the physical characteristics of the cream. Increasing the concentration of span 80 and tween 80 in cream preparations can increase viscosity and adhesion but reduce the spreadability. Antioxidant activity of jackfruit leaf ethanol extract obtained from F1 was 23.17%, F2 37.89% and F3 29.82%, significantly different with F(-) was 6.65%,. Variation in concentrations of span 80 and tween 80 do not affect their antioxidant activity. Keywords: Antioxidant, Jackfruit leaf, DPPH, Cream
VALIDASI METODE PENETAPAN KADAR ISONIAZID DAN VITAMIN B6 MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI SERTA APLIKASINYA DALAM SEDIAAN SIRUP OBAT TUBERKULOSIS Aqnes Budiarti; Nimas Yuniarsih Herdiyanti
CENDEKIA EKSAKTA Vol 3, No 2 (2018)
Publisher : Universitas Wahid Hasyim

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.3194/ce.v3i2.2472

Abstract

Isoniazid (INH) dan piridoksin HCl atau vitamin B6 (vit B6) dikenal sebagai obat kombinasi untuk tuberkulosis. Adanya metode penetapan kadar kedua zat ini secara simultan sangat diperlukan dalam uji stabilitas dan studi nasib obat dalam tubuh. Tujuan penelitian ini adalah mengembangkan metode penetapan kadar INH dan vit B6 menggunakan kromatografi cair kinerja tinggi (KCKT)serta aplikasinya dalam sediaan sirup tuberkulosis. Pengembangan metode menggunakan KCKT Jasco dengan detektor UV-Vis. Fase diam adalah C18  dan fase gerak berupa campuran dapar fosfat dan asetonitril (75:25, v/v) dengan waktu alir 1,2 mL/menit pada panjang gelombang 280 nm. Selanjutnya dilakukan uji validasi meliputi akurasi, presisi, selektivitas, linieritas dan sensitivitas. Metode analisis diaplikasikan pada dua sediaan sirup dari pabrik yang berbeda. Hasil penelitian menunjukkan bahwa metode teliti dengan %RSD 0,48 untuk INH dan 0,64% untuk vit B6. Metode akurat dengan % perolehan kembali kedua  zat antara 98-102%, metode linier dengan nilai r = 0,99 untuk kedua zat. Nilai LoD dan LoQ untuk penetapan kadar INH adalah 2,65 dan 8,85 ppm serta vit B6 adalah 0,33 dan 1,11 ppm. Uji selektifitas  menghasilkan nilai resolusi 4,8. Kadar INH  dan vit B6 masing-masing dalam sirup adalah 100,26-100,27% dan 100,00-100,85%. Metode analisis yang divalidasi dapat diaplikasikan pada sediaan sirup obat tuberkulosis. Kata kunci:INH, isoniazid, KCKT, sirup tuberkulosis, vit B6
IDENTIFIKASI KANDUNGAN SENYAWA KIMIA EKSTRAK ETANOL HERBA ALFALFA (Medicago sativa, L) Sri Susilowati; Maulita Cut Nuria; Agnes Budiarti
Media Farmasi Indonesia Vol. 9 No. 2 (2014): Media Farmasi Indonesia
Publisher : SEKOLAH TINGGI ILMU FARMASI YAYASAN PHARMASI SEMARANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.119 KB)

Abstract

ABSTRAK Alfalfa merupakan tumbuhan yang berasal dari Iran (Medicago sativa, L), namun dapat tumbuh di daerah tropis seperti Indonesia. Herba alfalfa secara umum dimanfaatkan sebagai pakan ternak, karena kandungan gizinya yang tinggi antara lain : protein, lemak dan serat kasar. Selain itu, alfalfa juga berfungsi sebagai tanaman berkhasiat obat untuk menyembuhkan berbagai penyakit. Tempat tumbuh dapat mempengaruhi kandungan senyawa kimia aktif dari suatu tanaman. Penelitian ini bertujuan untuk melakukan identifikasi secara kualitatif dan kuantitatif dari kandungan senyawa kimia herba alfalfa dalam bentuk sediaan ekstrak etanol. Sediaan ekstrak dibuat dengan metode perkolasi dengan pelarut etanol 96%. Identifikasi secara kualitatif dilakukan dengan pereaksi kimia yang dilanjutkan dengan Kromatografi Lapis Tipis terhadap senyawa yang diduga ada di dalam ekstrak yaitu flavonoid dan alkaloid serta kumarin. Identifikasi kuantitatif untuk senyawa flavonoid dilakukan secara spektrofotometri dengan panjang gelombang 505 nm, sedangkan alkaloid dilakukan dengan TLC densitometry dengan panjang gelombang 309 nm, dan kumarin secara TLC densitometry dengan panjang gelombang 304 nm. Hasil penelitian diperoleh ekstrak etanol herba alfalfa dengan rendemen sebesar 13,04%, dan memiliki kandungan flavonoid dengan kadar total 8,13%, alkaloid dengan kadar 48,86 ppm dan kumarin dengan kadar 229,83 ppm.
OPTIMASI FASE GERAK PADA ANALISIS CAMPURAN CIPROFLOXACIN HCL DAN METRONIDAZOL MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI Aqnes Budiarti; Ibnu Gholib Gandjar
Jurnal Ilmu Farmasi dan Farmasi Klinik Prosiding Seminar Nasional "Peranan dan Kontribusi Herbal dalam Terapi Penyakit Degeneratif"
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.383 KB) | DOI: 10.31942/jiffk.v0i0.377

Abstract

Penggunaan ciprofloxacin (CIP) untuk terapi penyakit infeksi saat ini sering dikombinasi dengan obat anti bakteri lain karena keterbatasannya melawan bakteri anaerob, salah satunya dengan metronidazol (MDZ). CIP berada dalam bentuk kationik pada kondisi asam (pH kurang dari 4,68) sehingga akan berikatan dengan residu silanol yang bermuatan negatif pada pH di atas 3,0, akibatnya dapat menyebabkan tailing. Hal ini dapat dicegah dengan penambahan reagen pasangan ion yang akan berikatan dengan residu silanol. Reagen pasangan ion yang sering digunakan dalam analisis senyawa kationik adalah amin tersier, seperti trietilamin (Anonim, 2010). Penelitian ini bertujuan untuk mengoptimasi fase gerak pada analisis campuran CIP.HCl dan MDZ menggunakan KCKT dengan penambahan trietilamin sebagai reagen pasangan ion. Fase diam yang digunakan adalah C18 dan fase gerak berupa campuran dapar fosfat 0,05 M dan asetonitril dengan perbandingan sesuai dengan hasil optimasi. Hasil penelitian menunjukkan bahwa pemisahan optimum diperoleh pada fase gerak berupa campuran dapar fosfat 0,05 M pH 3,5 dan asetonitril (80: 20, v/v) dengan kecepatan laju alir 1,2 mL/menit dan kadar trietilamin 0,05 Molar.   Kata kunci : Optimasi fase gerak, ciprofloxacin HCl,metronidazol,Kromatografi     Cair Kinerja Tinggi
PERBANDINGAN KADAR SUKROSA DALAM MADU RANDU DAN MADU KELENGKENG DARI PETERNAK LEBAH DAN MADU PERDAGANGAN DI KOTA SEMARANG Sumantri Sumantri; Aqnes Budiarti; Indah Parameita
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol.10 No.1 Juni 2013
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.089 KB) | DOI: 10.31942/jiffk.v10i1.867

Abstract

 ABSTRACT Key words: cottonwoods honey, longan honey, sucrose, KCKT   Honey is a natural liquid that generally has a sweet taste and produced by the bees. Along with the increase in consumption of honey, honey adulteration grew a way to get the benefits. Sucrose sugar often added because its price is relative cheap. The content of sucrose become a measurement of the authenticity of honey. The spurious honey harm consumers because of different composition so that its benefits are also different.The purpose of this research is to determine the sucrose content and the quantitation of sucrose in longan honey and honey cottonwoods from beekeepers and honey trade in the City of Semarang using HPLC.Honey samples were taken randomized with three sample of cottonwoods and three longan honey from beekeepers area in Ngaliyan, Gringsing dan Ambarawa and honey trade in the City of Semarang. The determination of sucrose using HPLC system (Waters ec 2695) equipped with C18 column (C18 5um Sunfire; 4.6 x150mm) and detector UV (UV/Visible Waters 2489) at wavelength 190 nm. Mobile phase consisted of a mixture of acetonitrile and aquabides (75:25, v/v) with a flow rate of 1.0 mL/min.Research results showed that the average content of sucrose in honey samples cottonwoods and longan honey from beekeepers respectively 4,03% dan 2,98%, while the average level for the sample of cottonwoods and longan honey trade in the City of Semarang respectively 2,11% and 2,59%. The average level of sucrose from twelve samples meet requirements but the there are two samples that does not comply requirement of honey based on SNI-2004. There was no significant difference between the levels of sucrose honey cottonwoods and honey longan from beekeepers and honey trade in the City of Semarang.
VALIDASI METODE ANALISIS KLORFENIRAMIN MALEAT DAN GUAIFENESIN MENGGUNAKAN KROMATOGRAFI CAIR KINERJA TINGGI SERTA APLIKASINYA DALAM SEDIAAN SIRUP Aqnes Budiarti; Ira Afifah
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol 14 No 1 juni 2017
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.917 KB) | DOI: 10.31942/jiffk.v14i1.1771

Abstract

INTISARI  Kombinasi  klorfeniramin  maleat (CTM) dan guaifenesin (GG) digunakan  untuk meringankan  gejala  batuk  dan  pilek.  Kadar CTM dan GG dalam sirup sangat kecil sehingga penetapan kadarnya memerlukan metode  yang valid. Tujuan penelitian ini adalah memvalidasi metode penetapan kadar CTM dan GG dan mengaplikasikannya dalam beberapa sediaan sirup di pasaran. Penetapankadar CTM dan GG menggunakan KCKTJascoLc-Net II/ADC dengandetektor UV-Visible. Fase  diam  berupa  C18Li  Chospher  dan fase gerak  adalah  campuran asetonitril:metanol:air (15:10:75 v/v), waktu alir 1 mL/menit  pada  panjang  gelombang 270 nm. Validasi metode meliputi akurasi, presisi, selektivitas, linieritas  dan  sensitivitas. Metode analisis diaplikasikan terhadap 3 merk sirup dari pabrik yang berbeda. Hasil penelitian menunjukkan bahwa validasi metode penetapan kadar CTM dan GG memenuhi persyaratan yaitu: uji presisi menghasilkan %RSD untuk CTM   0,18% dan GG 0,17%;ujiakurasi menghasilkan perolehan kembali  yang memenuhi  persyaratan  baikuntuk CTM maupun GG  pada sirup  ketigamerksirup. Selektivitasnya baik; linieritas memenuhi syarat;  LOD CTM dan GG masing masing sebesar 2,40 µg/mL dan 3,07 µg/mL; LOQ CTM dan GG masing-masing sebesar 8,00 µg/mL dan  10,24 µg/mL. Kadar rata-rata CTM  dan GG  pada  ketiga  sirup memenuhi persyaratan kadar yang ditetapkan oleh Farmakope Indonesia Edisi V. Kata kunci : validasi, klorfeniramin maleat, guaifenesin, KCKT ABSTRACT   The combination of CTM and GG is often used as an active  substances  to relieve symptoms of cough and cold. The concentration of CTM and GG in syrup are very low, so that it need validated determination method to ensure therapeutic dose. The purposes of this study were to validate determination method of CTM and GG and to apply the method to some syrups.   Determination of CTM and GG in syrup using HPLC JascoLc-Net II/ADC equipped with a UV-Visible detector at a wavelength of 270 nm. The stationary phase used was C18Li Chosphera nd the mobile phase usedwas a mixture of acetonitrile:methanol:water (15:10:75 v/v) with a flow rate of 1 mL/minutes.Validation test was conducted on precision test, accuracy, selectivity, linearity and sensitivity. The method of analysis was applied to syrups fromthree different factories.   The result of this research showed that validation tests  of CTM and GG  met  the requirements, were: %  RSD of precision test for CTM was  0.18% and  for GG was  0.17%; accuracy test resulted good  recovery for CTM and for GG  in  syrups. Good  selectivity; linearity with a correlation coefficient 0.999; LOD  for CTM and GG were 2.40 and 3.07 µg/mL; LOQ for CTM and GG were 8.00µg/mL and 10.24  µg/mL.  The level of  CTM  and GG    in syrups  were compliance with the requirements by the Indonesian Pharmacopeia Edition V. Keywords: validation, chlorfeniramin maleat, guaifenesin, HPLC
ANALISIS KANDUNGAN LOGAM BERAT TIMBAL (Pb), KADMIUM (Cd) DAN MERKURI (Hg) DALAM CUMI-CUMI (Loligo Sp.) YANG DIPEROLEH DARI TPI TAMBAK LOROK SEMARANG aqnes budiarti; rosmini rosmini; henna rya sunoko
Jurnal Ilmu Farmasi dan Farmasi Klinik JURNAL ILMU FARMASI DAN FARMASI KLINIK VOL. 5 NO. 1 JUNI 2008
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (133.426 KB) | DOI: 10.31942/jiffk.v5i1.826

Abstract

ABSTRACTThe research about analysis of heavy metal lead (Pb), cadmium (Cd) and mercury (Hg) in Octopuses (Loligo sp.) obtained from TPI Tambak Lorok Semarang has been carried out. This research was intended to identify heavy metals accumulating in octopuses (Loligo sp). The objects studied in this research were octopuses obtained from Tambak Lorok marketplace in Semarang. The sample for this research was taken by simple random sampling at three different points of locations. The locations 1, 2 and 3 were located each from the west, the north and the east, respectively, from the port. The contents of lead, cadmium and mercury in the octopuses’ bodies were measured using SSA (Atom-Absorption Spectrophotometer). The obtained data were then tested using One-way anava. The differences in metal contents were analyzed using Scheffe’s tests and then compared with the basic quality standard stipulated in the Decision Number 03725/B/SK/VII/1989 by the POM Directorate General The study based on the three different points of locations showed the following results. The average lead contents in the octopuses obtained from the points of locations 1, 2 and 3 were 0.29 mg/kg, 0.24 mg/kg and 0.25 mg/kg respectively. The average cadmium contents in the octopuses obtained from the points of locations 1, 2 and 3 were 0.04 mg/kg, 0.05 mg/kg and 0.06 mg/kg respectively. The average mercury contents in the octopuses obtained from the points of locations 1, 2 and 3 were 0.06 mg/kg, 0.07 mg/kg and 0.07 mg/kg respectively The lead, cadmium and mercury contents in the octopuses obtained from the three locations had not exceeded upper limit stipulated in the Decision Number 03725/B/SK/VII/1989 by the POM Directorate General. Based on the One-way ANOVA statistical tests on the lead contents, there was no significant difference among the three samples, but the statistical tests on the cadmium and mercury contents showed a significant difference among the three different points of locations relating to these heavy metal contents in the octopuses Keywords : SSA (Atomic-Absorption Spectrophotometer), Lead (Pb), Cadmium (Cd), Mercury (Hg), Octopuses (Loligo sp), Heavy Metal
KAJIAN KUALITAS AIR SUMUR SEBAGAI SUMBER AIR MINUM DI KELURAHAN GUBUG KECAMATAN GUBUG KABUPATEN GROBOGAN Aqnes Budiarti; Rupmini Rupmini; Henna Rya Soenoko
Jurnal Ilmu Farmasi dan Farmasi Klinik Jurnal Ilmu Farmasi & Farmasi Klinik Vol.10 No.1 Juni 2013
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.544 KB) | DOI: 10.31942/jiffk.v10i1.868

Abstract

 ABSTRACT Most of Grobogan people use well water facilities to fulfill water requirement every day. This study aim to evaluate well water quality that used as water drinking resourses in Gubug Grobogan and to determine well water meet the requirement as Regulation of Health Minister No. 416/Menkes/Per/IX/1990. This study is non-experimental research with descriptive analysis. Parameter of water quality that investigated comprised physical parameter, chemical parameter and microbiological parameter. Sample have been taken by simple random sampling from three types of well in Gubug, that is well near the garbage disposal, well near the Gubug village river which all along the river there are tofu factory "Bintang Timur" and syrup factory "Kartika", and well which were far from garbage disposal and gubug village river. Samples were determined by atomic absorption spectrofotometry for cadmium parameter dan spectrofotometry for chromium parameter, and double tube multiplied method for microbiologycal parameter. Physical parameter were analysed by colorimetry, termometry, turbidimetry and TDS meter. The results showed that well water quality in Gubug Grobogan based on physical parameter have appropiated with standard but well near the Gubug village river have not appropiated with colour standard. Based on chemical parameter, the three type of well have appropiated with standard for cadmium and chromium concentration. While based on mikrobiologycal parameter Coliform total have not appropiated with standard.Key words : quality study, well water, water drinking
ANALISIS KADAR TIMBAL (Pb) DAN MERKURI (Hg) PADA IKAN PINDANG SALEM (Scomber australasicus) YANG DIPEROLEH DARI TIGA PASAR TRADISIONAL TERBESAR DI KOTA SEMARANG Aqnes Budiarti; Listyowati Listyowati; Henna Rya Soenoko
Jurnal Ilmu Farmasi dan Farmasi Klinik JURNAL ILMU FARMASI DAN FARMASI KLINIK VOL. 7 NO. 2 DESEMBER 2010
Publisher : Universitas Wahid Hasyim Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.084 KB) | DOI: 10.31942/jiffk.v7i2.641

Abstract

ABSTRACT Smoked fish of Salem (Scomber australasicus) is kind of processing fish that well known in Indonesia. This study aim to detect presence of plumbum (Pb) and mercury (Hg) on smoked fish of Salem from traditional markets in Semarang. After determined its concentration, plumbum and mercury concentration in smoked fish of Salem were evaluated what were reached permissible limits based on standard of General Director Food and Drug Monitoring No. 03725/B/SK/VII/1989. This study is non-experimental research that analysed by analitycal descriptive method. Sample have been taken by simple random sampling from 9 sellers at three traditional markets in Semarang City that are Johar, Peterongan and Karangayu markets. Variabel which analysed were plumbum and mercury concentrations in smoked fish of Salem. Data were collected by laboratory test with Atomic Absorption Spectrophotometer. The results showed that smoked fish of Salem from three biggest traditional market in Semarang contained plumbum and mercury. Plumbum concentration on smoked fish of Salem from Johar market is 0.0633 ± 0.00577 mg/kg, from Peterongan market is 0.0600 mg/kg, and from Karangayu market is 0.0633 ± 0.00577 mg/kg. While mercury concentration on smoked fish of Salem from the three market are <0.01 mg/kg. Plumbum and mercury concentration on smoked fish of Salem from Johar, Peterongan, and Karangayu markets are not significant different. Plumbum and mercury concentration that accumulated on smoked fish of Salem from the three markets are not greater than quality standard of General Director of Food and Drug Monitoring No. 03725/B/SK/VII1989 about maximum restriction of metal pollution in food for fish and it processing result. Key words: smoked fish of Salem, determination, plumbum, mercury