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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Jurnal Ilmu Pertanian Jurnal Penelitian Saintek Agrikultura Zuriat Jurnal Fakultas Pertanian Buletin Penelitian Tanaman Rempah dan Obat Jurnal Agro Kultivasi Jurnal Sains Teh dan Kina MEDIAN AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Paspalum: Jurnal Ilmiah Pertanian jurnal Ilmu Pertanian (Agricultural Science) AGROLOGIA : Jurnal Ilmu Budidaya Tanaman Buletin Penelitian Tanaman Rempah dan Obat Jurnal Kajian Budaya dan Humaniora (JKBH)
Noladhi Wicaksana
Departemen Budidaya Pertanian, Fakultas Pertanian, Universitas Padjadjaran
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Mechanical Engineering Other

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Variability of fruit shelf-life of commercial tomatoes and their hybrids crossing with insensitivity ethylene mutant tomato Sletr1-2 Wiguna, Gungun; Bachtiar, Nedya Putri; Anas, Anas; Wicaksana, Noladhi; Mubarok, Syariful; Saadah, Imas Rita
Jurnal Ilmu Pertanian Vol 9, No 1 (2024): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.82772

Abstract

Tomatoes are climacteric fruits that experience a surge in respiration rate and ethylene production. This condition affects the fruits’ physiological deterioration, shortens their shelf-life, and degrades the quality of tomatoes. The study aimed to enhance the shelf-life of commercial tomatoes with different genetic backgrounds by utilizing the Sletr1-2 mutant, which has a lower sensitivity to ethylene. A randomized block design (RBD) was applied to achieve this goal, where 12 different plant genotypes were considered as treatments, and each treatment was replicated four times.  The results showed that fruit shelf-life was extended by crossing commercial tomatoes with Sletr1-2 mutant tomatoes. Genetic variability for all shelf-life characteristics were narrow. In contrast, there were broad phenotypic variability for fruit hardness and weight loss characteristics.
Heterologous expression of maize-derived antimicrobial peptide ZmES4 in Escherichia coli for potential plant pathogen control Amien, Suseno; Nurdiana, Dadi; Istifadah, Noor; Wicaksana, Noladhi; Salsabila, Haifanisa
Kultivasi Vol 24, No 1 (2025): Jurnal Kultivasi
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v24i1.62598

Abstract

ZmES4 is a plant-derived antimicrobial peptide (AMP) from maize that shows promise as a biocontrol agent against plant pathogenic organisms. In the context of growing challenges in sustainable agriculture, AMPs like ZmES4 represent innovative alternatives to chemical pesticides. This study focuses on the structural characterization and heterologous expression of the ZmES4 peptide in Escherichia coli (E. coli). The gene encoding ZmES4 was obtained from the maize female gametophyte (NCBI Reference Sequence: NM_001112150.3) and cloned into the pET24d(+) expression vector using NcoI and XhoI restriction sites. Transformation into E. coli BL21 (DE3) cells enabled recombinant expression upon induction with isopropyl β-D-1-thiogalactopyranoside (IPTG). Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Bradford assays confirmed the expression of ORF-ZmES4, with protein concentrations ranging from 14.647 to 63.606 mg/mL. The successful expression of ZmES4 in E. coli highlights its potential application as a recombinant AMP for future plant disease management strategies.
PENINGKATAN PEMAHAMAN DAN PENERAPAN PERTANIAN ORGANIK DI DESA JAGABAYA, KECAMATAN CIMAUNG, KABUPATEN BANDUNG: PENINGKATAN PEMAHAMAN DAN PENERAPAN PERTANIAN ORGANIK DI DESA JAGABAYA, KECAMATAN CIMAUNG, KABUPATEN BANDUNG Amien, Suseno; Widayat, Dedi; Wicaksana, Noladhi
Jurnal Kajian Budaya dan Humaniora Vol 4 No 2 (2022): Jurnal Kajian Budaya dan Humaniora (JKBH), Juni 2022
Publisher : PT. RANESS MEDIA RANCAGE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.61296/jkbh.v4i2.23

Abstract

Komoditas Padi merupakan salah satu komoditas andalan di Desa Jagayabaya, Kecamatan Cimaung Kabupaten Bandung. Kesadaran masyarakat akan pentingnya bahan pangan organik terus meningkat. Keterbatasan pengetahuan pertanian organik dan penerapannya di Desa Jagabaya telah mendorong dilaksanakannya pengabdian masyarakat untuk meningkatkan pemahaman dan pengetahuan pertanian organik termasuk pembuatan pupuk cair organik untuk meningkatkan produksi pangan organik khususnya padi. Metode partisipatif digunakan untuk melibatkan mitra secara aktif dalam pelaksanaan pemahaman dan penerapan pertanian organik. Tahap-tahap pelaksanaan terdiri dari sosialiasi kegiatan, wawancara, penyuluhan, diskusi, dan praktek. Hasil kegiatan terjadi peningkatan ilmu, pengetahuan dan teknologi pertanian organik, cara pembuatan pupuk cair organik serta penerapannya.
Variability of fruit shelf-life of commercial tomatoes and their hybrids crossing with insensitivity ethylene mutant tomato Sletr1-2 Wiguna, Gungun; Bachtiar, Nedya Putri; Anas, Anas; Wicaksana, Noladhi; Mubarok, Syariful; Saadah, Imas Rita
Jurnal Ilmu Pertanian Vol 9, No 1 (2024): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.82772

Abstract

Tomatoes are climacteric fruits that experience a surge in respiration rate and ethylene production. This condition affects the fruits’ physiological deterioration, shortens their shelf-life, and degrades the quality of tomatoes. The study aimed to enhance the shelf-life of commercial tomatoes with different genetic backgrounds by utilizing the Sletr1-2 mutant, which has a lower sensitivity to ethylene. A randomized block design (RBD) was applied to achieve this goal, where 12 different plant genotypes were considered as treatments, and each treatment was replicated four times.  The results showed that fruit shelf-life was extended by crossing commercial tomatoes with Sletr1-2 mutant tomatoes. Genetic variability for all shelf-life characteristics were narrow. In contrast, there were broad phenotypic variability for fruit hardness and weight loss characteristics.
Kekerabatan Genetik Populasi Bengkuang Pachyrhizus erosus Berdasarkan Karakter Morfologi Bunga dan Daun Agung Karuniawan; Noladhi Wicaksana
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 34 No. 2 (2006): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (149.915 KB) | DOI: 10.24831/jai.v34i2.1286

Abstract

Yam bean Pachyrhizus erosus is widely cultivated in Central America, Africa, Asia and Pacific.  The objective of the research was to analyse genetic relationships of yam bean originated from Indonesia compared to its ancestor species from Mexico and Guatemala. Fourty three yam beans consisted of 35 genotypes from Indonesia and 8 introduced genotypes from Mexico and Guatemala were planted under complete block design with two replications. Fourteen significant different traits from 25 morphological leaf and flower properties traits have been used to analyse the genetic relationships. Results showed that yam beans from Sumatra differed from its relatives from the rest regions of Indonesia. Yam bean populations originated from Indonesia are closely related to its ancestor species from Mexico and Guatemala.   Key words :  Yam bean, Pachyrhizus erosus, genetic relationships
Genetic diversity and rhizome flesh color variation among indonesian turmeric (Curcuma longa L.) accession revealed by SSR markers Amien, Suseno; Anggraeni, Marisa; Karuniawan, Agung; Wicaksana, Noladhi
Kultivasi Vol 25, No 2 (2026)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kultivasi.v25i2.71036

Abstract

Turmeric (Curcuma longa L.) is a rhizomatous plant widely cultivated in Indonesia. Although recent studies have demonstrated that simple sequence repeat (SSR) markers are highly effective for assessing genetic diversity in Curcuma longa germplasm, most investigations have focused either on molecular diversity or breeding-oriented germplasm characterization traits of agronomic without integrating phenotypic commercial importance, particularly rhizome flesh color. The study was conducted from February to May 2026. This study aimed to evaluate the genetic diversity, phylogenetic relationships, and rhizome flesh color variation among Indonesian turmeric accessions collected by Plant Breeding Laboratory at the Faculty of Agriculture, Universitas Padjadjaran, and was grown at the Ciparanje Experimental Farm. Eighteen turmeric accessions from various locations in Indonesia were analyzed using six SSR markers to assess genetic variation and phylogenetic relationships. The results showed that 12 polymorphic alleles were identified, with the highest polymorphism information content (PIC) value of 0.58 observed for the CuMiSat-35 primer. Principal coordinate analysis and cluster analysis divided the accessions into two main clusters, with Jaccard genetic distances ranging from 0.00 to 0.80, indicating genetic diversity among the 18 turmeric accessions tested. The accession pairs with the longest kinship distances were found between accession CL-93 (South Sulawesi) and several other accessions, such as CL-01, CL-20, CL-25, CL-34, CL-36, CL-38, CL-47, CL-61, and CL-84, with a Jaccard coefficient of 0.80 and a genetic similarity of 20%, indicating the presence of genetic variation. Eighteen turmeric accessions exhibited varying rhizome flesh colors, which illustrates the differences in curcumin content.
Co-Authors Achmad Baihaki Ade Ismail Ade Ismail Ade Ismail Agung Karuniawan Agung Rahmadi Agung Rahmadi Ahmad Shohibboniawan Ajang Maruapey Anas Anas Anas Anas Anas Anas Anas Anas, Anas Anggita Dewi Anggraeni, Marisa Anne Nuraeni Anne Nuraeni Anne Nuraini Ayu Fitriani Bachtiar, Nedya Putri Bambang Nurhadi Bayu Pradana Nur Rahmat D.W Utami Dedi Ruswandi Dedi Widayat Denny Sobardini Desy Lidiasari Dian Hidayat Edi Suryadi Elfan Waisimon Enceng Sobari, Enceng Erni Suminar Eryk Barlianto Farida Farida Feliatra Gungun Wiguna Hafsah Ashri Noor Azizah Heri Syahrian Heri Syahrian Heri Syahrian, Heri Hiroshi Ezura Ineu Sulastrini Iteu M. Hidayat Iteu M. Hidayat Jajang Supriatna Janwar Eka Saputra Joko Pinilih Kusumiyati Liberty Chaidir M. Khais Prayoga Maura Zhafira Putri Meddy Rachmadi Mita Indriani Moh Ali Abdullah Muhamad Joddy Ramadhan Muhamad Kadapi Muhammad Kadapi Muhammad Syafii Muhammad Syafii Neni Rostini Nono Carsono Noor Istifadah Nurdiana, Dadi Nurmalita Waluyo Nurmalita Waluyo Nursuhud Nursuhud Nyimas Poppi Indriani Priyanto, Slamet Bambang Putri Ardhya Anindita Qurrota Aini Reisyi Rinola Tambunan Riski Gusri Utami Riski Gusri Utami Riski Gusri Utami Rodiah, S S Rodiah Saadah, Imas Rita Safarinda Nurdianawati Salsabila, Haifanisa Santika Sari Santika Sari Santika Sari Shohibboniawan, Ahmad Siti Rakhmah Tenrisui Pakki Siti Rakhmah Tenrisui Pakki Siti Rodiah Slamet Bambang Priyanto Sulistyaningsih Sulistyaningsih Sumadi Sumadi Sumadi Sumadi SUMADI SUMADI Suseno Amien Syariful Mubarok Tarkus Suganda Tresna Kusuma Putri Vergel Concibido Wahyu Ferdiyansyah Wawan Sutari Yoana Saragih Yudithia Maxiselly Yuyun Yuwariah Z. Daulati Z. Daulati, Z. Zulfatunnisa Zulfatunnisa