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All Journal HAYATI Journal of Biosciences Maspari Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Saintek Perikanan : Indonesian Journal of Fisheries Science and Technology Journal of Marine Research Maspari Journal Jurnal Kelautan Tropis Panrita Abdi - Jurnal Pengabdian pada Masyarakat JFMR (Journal of Fisheries and Marine Research) Jurnal Perikanan Universitas Gadjah Mada Jurnal Intek Akuakultur Biosaintifika: Journal of Biology & Biology Education Jurnal Riset Akuakultur Aquacultura Indonesiana Journal of Tropical Marine Science
Ervia Yudiati
Department Of Marine Science, Faculty Of Fisheries And Marine Science, Diponegoro University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Engineering Environmental Science Immunology & microbiology Other

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Bulu Babi Pada Ekosistem Karang dan Lamun di Perairan Taman Nasional Karimunjawa The Michael Febrian Wijaya; Chrisna Adhi Suryono; Ervia Yudiati
Journal of Marine Research Vol 11, No 3 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i3.34055

Abstract

Bulu babi merupakan salah satu biota Echinodermata yang ditemukan di perairan Taman Nasional Karimunjawa. Bulu babi pada umumnya menghuni ekosistem terumbu karang dan ekosistem padang lamun serta menyukai substrat yang agak keras terutama substrat di padang lamun yang merupakan campuran dari pasir dan pecahan karang. Karakteristik yang berbeda pada kedua ekosistem akan mempengaruhi populasi pada ekosistem tersebut. Tujuan penelitian ini untuk mengetahui perbedaan kelimpahan bulu babi di ekosistem karang dan padang lamun di Pantai Legon Bajak dan Pulau Menjangan Kecil, Taman Nasional Karimunjawa. Metode yang digunakan dalam pengambilan data penutupan karang adalah transek kuadrat dengan skala sepanjang 50 meter dan jarak antar transek yaitu 10 meter. Pengambilan data penutupan dan kerapatan lamun menggunakan transek kuadrat dengan ukuran 50 x 50cm. Kelimpahan bulu babi pada ekosistem terumbu karang dan padang lamun menggunakan Benthos Belt Transect (BBT). Hasil penelitian penutupan terumbu karang dan lamun di Pantai Legon Bajak dikategorikan rusak dengan persentase rata – rata 24,78% dan 20,1%. Sedangkan penutupan terumbu karang dan lamun di Pulau Menjangan Kecil dikategorikan sedang dengan nilai persentase rata – rata 49.16% dan 34.7%. Hubungan korelasi antara penutupan karang dengan kelimpahan bulu babi termasuk dalam kategori sangat kuat dengan nilai koefisien r sebesar 0,814, sedangkan nilai hubungan korelasi antara penutupan lamun dengan kelimpahan bulu babi termasuk dalam kategori sedang dengan nilai koefisien r sebesar 0,585. Sea urchins are one of the Echinoderms found in the Karimunjawa National Park waters. Sea urchins mostly inhabit coral reef ecosystems and seagrass beds ecosystems and like a rather hard substrate, especially the substrate in seagrass beds which is a mixture of sand and coral rubble. The different characteristics in the two ecosystems will affect the population in that ecosystem. The purpose of this research was to determine differences in the abundance of sea urchins in coral and seagrass ecosystems at Legon Bajak Beach and Menjangan Kecil Island, Karimunjawa National Park. The sampling method used in the coral cover data is quadrat methods with a scale of 50 meters and the distance between transects is 10 meters. Seagrass cover and density data collection used 50 x 50 cm quadrant transect. The abundance of sea urchins in coral reef and seagrass ecosystems using Benthos Belt Transect (BBT). The research results showed that the cover of coral reefs and seagrass beds in Legon Bajak Beach were categorized as bad which are of 24.78 % on coral reef and 20.1 % seagrass. Meanwhile, the cover of coral reefs and seagrass beds in Menjangan Kecil Island in the category of moderate which are of 49,16 % and 34,7 %. The correlation between coral cover and sea urchin abundance is in the very strong category with an r coefficient of 0.814, while the correlation between seagrass cover and sea urchin abundance is in the medium category with an r coefficient of 0.585.
Potensi Ekstrak Buah Mangrove Xylocarpus granatum Untuk Pemberantasan Larva Nyamuk Aedes aegypti Delianis Pringgenies; Rini Widiyadmi; Ervia Yudiati; Muhammad Syaifudien Bahry; Ali Djunaedi
Journal of Tropical Marine Science Vol 1 No 1 (2018): Journal of Tropical Marine Science
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (405.368 KB) | DOI: 10.33019/jour.trop.mar.sci.v1i1.657

Abstract

ABSTRAK Tujuan penelitian adalah untuk mengetahui potensi ekstrak buah mangrove Xylocarpus granatum sebagai anti larva nyamuk Aedes aegypti. Uji larva nyamuk dengan memasukkan masing-masing 10 ekor larva nyamuk yang dimasukkan ke dalam 7 botol cup tranparan kecil, masing– masing untuk 50, 100, 250, 500, 1000 ppm sampel ektrak, kontrol negative dan kontrol positive. Hasil penelitian memperlihatkan bahwa pengaruh tingkat mortalitas larva nyamuk terhadap ekstrak buah mangrove Xylocarpus granatum pada jam 1 memperlihatkan bahwa pada konsentrasi 1000 ppm, persentasi mortalitas nyamuk tertinggi adalah 81,34 %, dan pada konstrasi ekstrak buah mangrove Xylocarpus granatum 100 ppm terendah mortalitasnya = 68,8%. Perlakuan pada jam 3 memperlihatkan bahwa pada konsentrasi ekstrak buah mangrove 500 ppm tingkat mortalitas larva nyamuk tertinggi (80%). Pada konsentrasi ekstrasi buah mangrove 250 ppm, mortalitasnya 66,70% dan konsentrasi ekstral buah mangrove pada 100 ppm, mortalitasnya adalah 40%. Hasil peneltian pada jam ke 24 memperlihatkan bahwa pada konsentrasi ekstrak buah mangrove mencapai 1000 pmm, mortalitasnya 100%, pengamatan jam ke 48 mortalitasnya 100%. Sedang pada perlakuan control positif, keluulushidupan larva nyamuk adalah 0% dan pada control negative, kelulushidupan larva nyamuk adalah 100%. Kesimpulan: bahwa ekstrak buah mangrove Xylocarpus granatum berpotensi sebagai anti larva nyamuk pada konsentrasi 1000 ppm. ABSTRACT It was assumed that mangrove fruits has some insecticidal biosubstances. Aim of the research is the potentials of mangrove Xylocarpus granatum fruit extract as the anti mosquito (Aedes aegypti) repellent. Experiment using of 10 mosquito larvae in 7 bottles each filled with 50; 100; 250; 500 and 1000 ppm fruit extract, negative and positive control. The experiment reveals that after 1 hour treatment, the 1000 ppm exctract had the hihgest mosquito larvae mortality of 81.34%, while the lowest extract of 100 ppm had 68.8% mortality. After 3 hours of extract treatment the 500 ppm had the hihgest mortality of 80%, 250 ppm with 66.70% and 100 ppm with 40 % mortality. Result of experiment after 24 as well as 48 hours treatment the 1000 ppm extract had 100% mortality. In the positive control had 0% mortality and the negative control had 100% of survival. The summary is that the mangrove Xylocarpus granatum fruit extract had a potential as mosquito repellent at 1000 ppm fruit extract.
Pengaruh Pertumbuhan Spirulina platensis terhadap Kandungan Pigmen beda Salinitias Dieng Widawati; Gunawan Widi Santosa; Ervia Yudiati
Journal of Marine Research Vol 11, No 1 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i1.30096

Abstract

Spirulina platensis merupakan mikroalga hijau-kebiruan dalam kelas Cyanophyceae yang mengandung klorofil-a dan fikobiliprotein. Faktor lingkungan yang dapat mempengaruhi pertumbuhan dan pigmen Spirulina platensis salah satunya yaitu salinitas. Penelitian ini bertujuan untuk mengetahui pertumbuhan dan kandungan pigmen mikroalga Spirulina platensis pada salinitas yang berbeda. Metode penelitian yang digunakan adalah eksperimental laboratoris dengan menggunakan Rancangan Acak Lengkap. Hasil penelitian menunjukkan kepadatan mikroalga tertinggi pada (salinitas 15 ppt) sebesar 211.875±1994 unit/mL dan terendah pada salinitas 25 sebesar 141.539±5872 unit/mL. Laju pertumbuhan tertinggi didapat pada salinitas 20 ppt sebesar 0,327±0,019 unit/hari dan terendah pada salinitas 25 ppt sebesar 0,246±0,012 unit/hari. Kandungan klorofil-a berkisar antara 10,622±1,322 µg/mLpada salinitas 30 ppt dan 8,176±2,426 µg/mL pada salinitas 15 ppt. Kandungan fikosianin berkisar antara 0,105 ± 0,041 mg/mL (salinitas 20 ppt) sampai 0,058 ± 0,005 mg/mL (salinitas 30 ppt). Allofikosianin berkisar antara 0,069±0,010 mg/mL pada salinitas 20 ppt sampai 0,042±0,007 mg/mL pada salinitas 30 ppt. Kisaran fikoeritrin antara  0,384±0,159 mg/mL pada salinitas 20 ppt sampai 0,239±0,014 mg/mL pada salinitas 30 ppt. Berdasarkan hasil penelitian disimpulkan bahwa salinitas memiliki pengaruh signifikan terhadap pertumbuhan dan laju pertumbuhan, namun tidak pada kandungan pigmen mikroalga Spirulina platensis. Kandungan klorofil a dan fikobiliprotein yang terdiri dari fikosianin, allofikosianin dan fikoeritrin, tidak menunjukkan perbedaan yang signifikan pada salinitas yang berbeda. Spirulina platensis is a blue-green microalga in the Cyanophyceae class that contains chlorophyll-a and phycobiliprotein. One of the environmental factors affecting the growth and pigment of Spirulina platensis is salinity. This study aims to determine the growth and pigment content of Spirulina platensis at different level of salinity. The research was carried out from March till April 2020 at the Marine Biology Laboratory and Marine Chemistry Laboratory, Building E, Faculty of Fisheries and Marine Sciences, Diponegoro University, Semarang. The research method used was a laboratory experiment using a completely randomized design. The results showed that the highest microalgae density achieved at salinity 15 ppt as 211.875±1994 units/mL, meanwhile the lowest was gained at salinity 25 ppt at 141.539 ± 5872 units/mL. The highest growth rate was obtained at 0.327 ± 0.019 unit/day at salinity 20 ppt, and the lowest was achived at 0.246 ± 0.012 unit/day at salinity 25 ppt.  The chlorophyll content ranged from 10.622 ± 1.322 µg/mL at salinity 30 ppt and 8.176 ± 2.426 µg/mL at salinity 15 ppt. The phycocyanin content ranged from 0.105 ± 0.041 mg/mL at salinity 20 ppt to 0.058 ± 0.005 mg/mL at salinity 30 ppt. Allophycocyanin ranged from 0.069 ± 0.010 mg/mL at salinity 20 ppt to 0.042 ± 0.007 mg/mL at salinity 30 ppt, and phycoerythrin ranged from 0.384 ± 0.159 mg/mL at salinity 20 ppt to 0.239 ± 0.014 mg/mL at salinity 30 ppt. The results suggested that salinity had a significant effect (p<0.05) on density biomass and growth rate of Spirulina platensis microalgae, but did not influence on pigment concentration. Measurements of chlorophyll-a and phycobiliprotein content, including phycocyanin, allophycocyanin, and phycoerythrin indicated that salinity did not affect the pigment concentration of microalgae Spirulina platensis.
Antioxidant Activity and Bioactive Compounds of Tropical Brown Algae Padina sp. from Bintan Island, Indonesia Jelita Rahma Hidayati; Muhammad Syaifudien Bahry; Ita Karlina; Ervia Yudiati
Jurnal Kelautan Tropis Vol 25, No 3 (2022): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v25i3.15562

Abstract

Macroalgae is one of the marine resources that have the ability as an antioxidant. Its ability is obtained from bioactive compounds produced through secondary metabolism. One type of macroalgae that has the potential as an antioxidant is Padina sp. This study aims to analyze the content of bioactive compounds in Padina sp and determine their antioxidant activity using DPPH as free radicals. Padina sp. was taken from the Bintan waters and shade-dried for three days. Bioactive compounds were analyzed through phytochemical screening to determine the content of flavonoid compounds, steroids, triterpenoids, saponins, and tannins. Determination of antioxidant activity begins with measuring the maximum wavelength of DPPH at 400-800 nm and determining the incubation time of the sample and DPPH. Antioxidant activity was determined based on the value of Inhibition concentration (IC50) at a wavelength of 515.5 nm; total phenolic content was determined using gallic acid standard (725 nm); total flavonoid content was determined using quercetin standard (415 nm), chlorophyll a and carotenoids were selected to determine pigment content on the sample. The results showed that Padina sp. contains flavonoid compounds, steroids, and tannins. Extract of Padina sp. has a total phenolic content of 46.02 mg/GAE g; total flavonoid content of 35.36 mg/QE g; chlorophyll content of 9.18 mg/g; and carotenoid content of 26.46 µmol/g. Methanol extract of Padina sp. has an IC50 value of 92.17 ppm and is classified as a strong antioxidant.
Peningkatan Pertumbuhan Artemia sp. melalui Aplikasi Ekstrak Ulva sp. Natasya Erdza Aulia; Ervia Yudiati; Retno Hartati
Journal of Marine Research Vol 12, No 2 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i2.34629

Abstract

Kandungan gizi pada Artemia sp. merupakan salah satu faktor yang mempengaruhi penggunaannya Artemia sp. sebagai pakan alami pada budidaya biota laut. Ulva sp. memiliki potensi sebagai pakan Artemia sp. karena memiliki kandungan protein dan karbohidrat yang tinggi dan keberadaannya melimpah di Indonesia. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak Ulva sp. terhadap pertumbuhan Artemia sp. Metode penelitian yang digunakan adalah eksperimental menggunakan rancangan acak lengkap dengan berbagai konsentrai ekstrak Ulva sp. sebanyak 3 pengulangan. Laju pertumbuhan spesifik (SGR) dan laju pertumbuhan (GR) diukur setiap hari selama 5 hari, dan nilai toksisitas (LC50) menggunakan metode BSLT pada 24 jam dengan mengaplikasikan ekstrak Ulva sp. kepada Artemia sp. Hasil uji toksisitas dengan nilai LC50 sebesar 59603.40 ppm yang menunjukkan ekstrak Ulva sp. tidak toksik. Hasil penelitian menunjukkan bahwa Artemia sp. yang dipelihara dengan pemberian ekstrak Ulva sp. konsentrasi 1500 ppm mempunyai nilai laju pertumbuhan spesifik (SGR) tertinggi (29.15%) dan laju pertumbuhan (GR) tertinggi (329.58 um/day). ANOVA test menunjukkan pengaruh pada perbedaan konsentrasi Ulva sp. terhadap pertumbuhan Artemia sp. yang berbeda nyata. Penelitian ini menghasilkan pemberian ekstrak Ulva sp. mempengaruhi peningkatan pertumbuhan Artemia sp. Pertumbuhan Artemia sp. yang dipelihara dengan konsentrasi 1500 ppm memiliki nilai pertumbuhan paling tinggi. The nutritional content of Artemia sp. is one of the factors that influence the use of Artemia sp. as natural food for marine biota cultivation. Ulva sp. has potential as feed for Artemia sp. because it has a high protein and carbohydrate content and is abundant in Indonesia. This study aims to determine the effect of Ulva sp. on the growth of the toxicity of Artemia sp. The research method used is experimental using a completely randomized design using Ulva sp. extract with different concentration in 3 repetitions. Specific growth rate (SGR) and growth rate (GR) were measured daily for 5 days, and toxicity value (LC50) using the BSLT method at 24 hours by applying extracts of Ulva sp. to Artemia sp. The results of the toxicity test with an LC50 value of 59603.40 ppm which shows the extract of Ulva sp. is not toxic. The results showed that Artemia sp. which were maintained by administration of Ulva sp. concentration of 1500 ppm had the highest specific growth rate (SGR) (29.15%) and the highest growth rate (GR) (329.58 um/day). ANOVA test showed the effect on differences in concentrations of Ulva sp. on the growth of Artemia sp. significantly different. This research resulted in the administration of Ulva sp. affect the growth of Artemia sp. Growth of Artemia sp. reared with a concentration of 1500 ppm had the highest growth value.
Potential of Sodium Alginate in Sargassum sp. in Lotion Preparation to Treat Incision Wound in Mice Amelia Cahya Angelina; Ervia Yudiati; Delianis Pringgenies
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.22539

Abstract

Sargassum sp. is a known genus of brown seaweed whose dominant component is alginic acid or alginate. Various studies found that alginate having proinflammatory activity, so it may have potential to improve the wound healing process. This study aimed to obtain sodium alginate extract and to know the potency of the sodium alginate extract in incision wound treatment in mice abdomen. Lotion application was performed once a day in a span of five consecutive days. Observation on the wound was carried out to obtain data on blood coagulation, tissue reparation, and fibroblast development rate on the wound using histology. The results showed that the average yield from the Sargassum sp. extraction process was 31.3%. Quality control of the prepared lotion included organoleptic test; light brown coloration, lotion specific odor, semi- solid texture. The acidity of the lotion was measured as 6.61 with 6.731 cp of viscosity. The lotion adhesiveness test showed 0.21 seconds and the lotion was shown to be oil-in-water. Application of the lotion on incision wounds made in the abdomen of rats showed blood coagulation on the first day and onset of wound reparation process on the second day. Wound observation by the third day showed that the inflicted area had undergone a near-complete reparation. The study also showed that on the fifth day of reparation Sargassum sp. extract lotion treatment group showed fibroblast formation, as opposed to the non-extract lotion treatment group. Previous study use alginates as wound dressing material, but in this study alginate used as active ingredient in wound healing treatment. Therefore, this study concludes that sodium alginate in Sargassum sp. seaweed has a potential application in the field of medicine.
Suplementasi Ekstrak Spirulina sp. Pada Pakan Meningkatkan Toleransi Post-Larva L. vannamei Terhadap Stres Salinitas Lestari, Raisha Fahmida Dwi; Yudiati, Ervia; Djunaedi, Ali
Journal of Marine Research Vol 13, No 1 (2024): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v13i1.37701

Abstract

Litopenaeus vannamei merupakan komoditas yang bernilai ekonomi tinggi pada sektor perikanan dan budidaya. Spirulina sp. merupakan mikroalga yang mengandung nutrisi tinggi dan senyawa potensial yang memiliki efek terapeutik. Penelitian ini bertujuan untuk mengetahui pengaruh suplementasi ekstrak Spirulina sp. dalam pakan terhadap tingkat kelangsungan hidup, pertumbuhan, dan tingkat kelangsungan hidup L. vannamei pada uji stres salinitas. Hewan uji yang digunakan adalah post-larvae (PL 8) L. vannamei dengan bobot rata-rata 0,002 g/ekor. Penelitian ini menggunakan metode Rancangan Acak Lengkap (RAL) yang terdiri atas 3 konsentrasi, yaitu 3 mg/kg (SP 3), 6 mg/kg (SP 6), dan 9 mg/kg (SP 9) serta satu kontrol dengan masing-masing 4 pengulangan. L. vannamei dipelihara dan diberi pakan suplementasi Spirulina sp. selama 14 hari. Pada akhir penelitian dilakukan uji stres salinitas dengan menggunakan tabung falcon yang masing-masing diisi 20 ekor PL dan dipaparkan pada akuades (45 mL) dan ditutup rapat. PL diamati dan dihitung jumlah kelulushidupannya setiap 10 menit. Waktu pengamatan dihentikan ketika kematian udang mencapai 50%.Hasil penelitian menunjukkan bahwa pemberian suplementasi ekstrak Spirulina sp. pada pakan tidak berpengaruh nyata (p>0,05) terhadap pertumbuhan dan tingkat kelangsungan hidup udang. Namun, perlakuan SP 9 menunjukkan pengaruh nyata (p<0,05) terhadap tingkat kelangsungan pada uji stres salinitas. Suplementasi ekstrak Spirulina sp. mampu meningkatkan toleransi L. vannamei terhadap stres salinitas.  Litopenaeus vannamei is high economically viable cultivate in the aquaculture sectors. Spirulina sp. is high nutrients microalgae and potential compound that have therapeutic effects. This study aims to determine the effect of dietary supplementation of Spirulina sp. on feed towards survival rate, growth, and resistance of L. vannamei in the salinity stress test. This experiment was using post-larvae (PL 8) (0.002 g/ind). This study used a completely randomized design (CRD) consisting of 3 concentrations, namely 3 mg/kg (SP 3), 6 mg/kg (SP 6), and 9 mg/kg (SP 9), and one control with 4 repetitions for each treatment.  L. vannamei was reared and fed with Spirulina sp. supplementation for 14 days. On stress salinity test, PL were put in the falcon tube filled with aqua dest with 20 PL each and exposed until 50% mortality. The results showed that the supplementation of Spirulina sp. on feed had no significant effect (p>0.05) on the growth and survival rate of shrimp. However, the SP 9 treatment showed a significant effect (p<0.05) on the survival rate in the salinity stress test. Is concluded that supplementation of Spirulina sp. in the diet managed to increase the resistance of L. vannamei toward stress salinity.
Suplementasi Alginat dan Spirulina Meningkatkan Ketahanan Udang Litopenaeus vannamei Terhadap Pajanan Salinitas Adella, Alisa Sukma; Yudiati, Ervia; Sedjati, Sri
Journal of Marine Research Vol 12, No 4 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i4.37937

Abstract

Permintaan pasar yang tinggi membuat Litopenaeus vannamei menjadi komoditas penting yang perlu ditingkatkan produksinya. Salah satu upaya peningkatan produksi budidaya udang adalah dengan memberikan pakan yang sesuai dengan kebutuhan. Penelitian ini bertujuan untuk mengetahui pengaruh dari suplementasi alginat dan Spirulina dalam pakan udang pada ketahanan terhadap tingkat kelangsungan hidup, stres salinitas, serta pertumbuhan panjang dan berat dari post-larvae L. vannamei. Metode penelitian dilakukan dengan suplementasi selama 14 hari yang terdiri dari K (kontrol), perlakuan AS 1 (Alg 1 g+Spr 1 mg/kg), perlakuan AS 2 (Alg 2 g+Spr 2 mg/kg), dan perlakuan AS 3 (Alg 3 g+Spr 5 mg/kg) dengan masing-masing 4 pengulangan. Tingkat kelangsungan hidup L. vannamei dihitung kemudian dilakukan uji stres salinitas dengan menempatkan udang dari media bersalinitas 25 ppt menjadi 0 ppt. Kelulushidupan udang dicatat setiap 10 menit hingga kematian mencapai >50% dari total udang keseluruhan. Hasil penelitian menunjukan tingkat kelangsungan hidup tertinggi diperoleh pada udang dengan perlakuan K (87,5%), diikuti AS 1 (85,5%), AS 2 (85%), dan AS 3 (79,5%). Ketahanan terhadap salinitas dengan waktu terlama secara berurutan terjadi pada udang dengan perlakuan AS 1 (100 menit), AS 2 (90 menit) serta K (80 menit) dan AS 3 (80 menit). Pertumbuhan panjang tertinggi terdapat pada AS 3 (0,940 cm), AS 2 (0,926 cm), AS 1 (0,897 cm), dan K (0,890 cm). Pertumbuhan berat tergolong sama pada K (0,023 g), AS 1 (0,023 g), dan AS 3 (0,023 g) dan berat terkecil terdapat pada perlakuan AS 2 (0,018 g).  High market demand makes Litopenaeus vannamei an important commodity that needs to be increased in production. One of the efforts to increase the production of shrimp aquaculture is to provide feed according to the needs. This study aims to determine the effect of alginate and Spirulina supplementation in shrimp feed on resistance to survival, salinity stress, and growth in length and weight of L. vannamei post-larvae. The research method was carried out by supplementation for 14 days consisting of K (control), AS 1 treatment (Alg 1 g+Spr 1 mg/kg), AS 2 treatment (Alg 2 g+Spr 2 mg/kg), and AS 3 treatments. (Alg 3 g+Spr 5 mg/kg) with 4 repetitions each. The survival rate of L. vannamei was calculated then a salinity stress test was carried out by placing the shrimp from media with a salinity of 25 ppt to 0 ppt. Shrimp survival was recorded every 10 minutes until death reached > 50% of the total shrimp. The results showed that the highest survival rate was obtained in shrimp treated with K (87.5%), followed by AS 1 (85.5%), AS 2 (85%), and AS 3 (79.5%). Resistance to salinity with the longest time sequentially occurred in shrimp treated with AS 1 (100 minutes), AS 2 (90 minutes) and K (80 minutes) and AS 3 (80 minutes). The highest growth in length was found in AS 3 (0.940 cm), AS 2 (0.926 cm), AS 1 (0.897 cm), and K (0.890 cm). Weight growth was the same in K (0.023 g), AS 1 (0.023 g), and AS 3 (0.023 g) and the smallest weight was found in AS 2 treatment (0.018 g).
Combination of Alginate and Ginger Oil as Edible Coating Formulation for Reducing Pathogenic Bacteria in Litopenaeus vannamei Amal, Ikhlasul; Yudiati, Ervia; Sedjati, Sri; Ighwerb, Mostafa Imhmed
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 28, No 4 (2023): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.28.4.313-320

Abstract

Litopenaeus vannamei is a world trade commodity that has a high economic value but is easily degraded. To maintain the quality, post-harvest peeled shrimp needs to be coated. The research objective was to determine the effectiveness of adding edible coatings with a formulation of alginate and ginger oil combination as a natural preservative for shrimp. The research method used was laboratory experimental with a completely randomized design (CRD). The treatments were additional peeled shrimp with different concentrations of alginate, i.e. 0 g, 1.5 g, and 3.0 g with two repetitions. The addition of 100 ml aquadest, 4 g CaCl2, 15 mL glycerol, and 1.5 mL of ginger oil was administered in every treatment. Total Plate Counts (TPC), Escherichia coli, and Vibrio cholerae were determined in initial condition, 3rd, and 6th days after the storage time in a 40C refrigerator.  The edible film test with 5 repetitions, including thickness, tensile strength, and break elongation. The results on TPC (5.00 x 105 CFU.g-1) and TPC of V. cholerae (Negative.25 g-1) show that all the treated shrimp meet the standard value (SNI 3457:2021) requirements. Most Probably Number of E. coli in alginate addition of 0 and 1.5 g i.e. <3 MPN.g-1 treatments, do not meet the requirements. The bacterial test and edible film mechanical properties showed that the addition of 3.0 g alginate was the most effective treatment compared to other treatments (α=0.05). A formulation of alginate and ginger oil combination is an effective edible coating for reducing the pathogenic bacteria of peeled shrimp L. vannamei.
The Impact of Varying Alginate Co-activation with Probiotics on the Artemia Bioencapsulation to Enhance Immunity Against Vibrio spp. Suryono, Chrisna Adhi; Santoso, Adi; Yudiati, Ervia; Yulianto, Bambang; Sunaryo, Sunaryo; Azhar, Nuril; Alghazeer, Rabia
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 3 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.3.372-384

Abstract

Alginate is known as an immunostimulant. The comprehensive study of Artemia on its co-activity with Lactobacillus bulgaricus resolves the relationship of feed digestibility, hematological parameters, gene expression, feed digestibility, and disease-resistant have not been covered. This study aimed to determine the effect of various doses alginate with Lactobacillus bulgaricus bio-encapsulated Artemia in Vibrio vulnificus, V. parahaemolyticus, and V. harveyi challenged. Alginate from Sargassum sp. L. bulgaricus were cultured and grown with de Man, Rogosa and Shape media. Nauplii Artemia was encapsulated for one hour with 400, 600, 800 ppm alginate doses, with and without probiotics. The Artemia then was challenged with three Vibrio spp. The Artemia mortality, immune parameters (Phenol Oxidase, Super-oxide Dismutase) were evaluated. Pro Phenol Oxidase, b-1,3-glucan-binding proteins (LGBP), and Lectin gene expression as well as gut evacuation time, fullness of gut were recorded.  Compared to the non-probiotic alginate, co- probiotic activated and improved the mortality rate of 400 ppm alginate from 100% to 30-70% (36 h), CMI of 276-702, and mortality reduction (41.21-74.59%). The co-activity of 400 ppm alginate and L. bulgaricus resulted in higher PO and SOD activity. LGBP and proPO gene expression were also upregulated 233.44% and 185.17%. The gut evacuation time and fullness of alginate 400 ppm and L. bulgaricus treatment have also resulted in better performance than those of 800 ppm alginate without probiotics. Alginate and L. bulgaricus probiotics cooperated synergically through pre and probiotic mechanisms. This Artemia bio model defines that this combination will improve the survival rate, immune system, and gene expression. In the future this will be beneficial in terms of shrimp production in ponds.
Co-Authors Abidin Nur II Adella, Alisa Sukma Adha Susanto Adi Santoso Agung Sudaryono Agus Setyawan Agus Trianto Akbar Harahap Alfi Satriadi Alghazeer, Rabia Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridlo Alifia Nabilla Putri Alim Isnansetyo Amalia Rachma Fatchiyyah Ambariyanto Ambariyanto , Ambariyanto Ambariyanto Ambariyanto Ambariyanto Amelia Cahya Angelina Angelina, Amelia Cahya ANIK KUSMIATUN Annisa Afifah Nugroho Arief Taslihan Arumning T. Fauziah Arumning Tias Fauziah Avigail, Yolanda Ayunda Ainun Nisa Azhar, Nuril Azhar, Riza Nur Bambang Yulianto Bambang Yulianto Chika Velita Anindya Yulian Chrisna A Suryono Chrisna Adhi Suryono Chrisna Adi Suryono Danang Ambar Prabowo Dea Shinta Kharisma Adziana Dedi . Dedi Setiawan Delianis Pringgenies Delianis Pringgenies Delianis Pringgenies Desrina Desrina DIAH AYU SATYARI UTAMI Diah Permata Wijayanti Diah Tri OktaviyantI Dieng Widawati Dina Ayu Magfirani Dinny Anjang Sari Diyah Putri Ambarwati Djoko Suprapto Dwi Wulandari Dwi Wulandari Edi Wibowo Endang Sri Susilo Endang Sri Susilo Endang Supriyantini Eny Heriyati Fadhliyah Idris Fanny Iriany Ginzel Girsang, Putri Hutari Gunawan Widi Santosa Gunawan Widi Santosa H. Endrawati Haeruddin Haeruddin Harahap, Akbar Hatim Albasri, Hatim Hilda Ayu Aprilia Hilda Ayu Aprilia Hotnida Junita Situmeang Ighwerb, Mostafa Imhmed Ikhlasul Amal Ilham Ilham Insafitri, I Insani, Liga Ipanna Enggar Irpan Hasibuan Irwani Irwani Ita Karlina Ita Riniatsih Ita Widowati Jelita Rahma Hidayati Johannes Hutabarat Langkah Sembiring Lestari, Raisha Fahmida Dwi Lilik Maslukah Linggar Dirgantara Prasetyo Magfirani, Dina Ayu Mila Safitri Rizfa Mila Safitri Rizfa Mostafa Imhmed Ighwerb Muhammad Salauddin Ramadhan Djarod Muhammad Salauddin Ramadhan Djarod Muhammad Syaifudien Bahry Muhammad Syaifudien Bahry Natasya Erdza Aulia Nuril Azhar Nuril Azhar Nuril Azhar Patria Ami Wijaya Puji Norbawa Putri Hutari Girsang Rabia Alghazeer Rabia Alghazeer Rabia Alghazeer Raden Bagus Sugio Sumanta Rahman Rahman Rani Agustian Rani Agustian Rizkina Rani Agustian Rizkina Retno Hartati Ria Azizah T.N. Ria Azizah Tri Nuraeni Rini Widiyadmi Rudhi Pribadi Rustadi Rustadi Saniyatul Ulya Sari, Alfianisa Permata Sarjito - Sarjito . Shofa Farihah Simanjuntak, Sri Devi Sri Devi Simanjuntak Sri Sedjati Sri Sedjati Sri Sedjati Sri Sejati Subagiyo Subagiyo Subagiyo Subagiyo Sunarsih Sunarsih Sunaryo Sunaryo Suryono Suryono Suryono Suryono Suryono Suryono Szuzter, Brian Walter The Michael Febrian Wijaya Tika Retnowati Triyanto Triyanto Valentina R. Iriani Wahyu Wahyu Widianingsih Widianingsih Widianingsih Widianingsih Wijaya, Patria Ami Yolanda Avigail Yulius Docang Casessar Zaenal Arifin Zaenal Arifin Zaenal Arifin Zaneb M. Ben Mansur