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Eka Kartika Untari
Departemen Farmasi, Fakultas Kedokteran, Universitas Tanjung Pura
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
Immunostimulant activity of soybean milk against immunoglobulin (IgG, IgA) and lymphocyte cells proliferation of Balb/c mice induced hepatitis A Ediati Sasmito; Sri Mulyaningsih; Eka Kartika Untari; Ratna Widyaningrum
Indonesian Journal of Pharmacy Vol 17 No 3, 2006
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.137 KB) | DOI: 10.14499/indonesianjpharm0iss0pp156-161

Abstract

Soybean milk contains of isoflavon aglycon as genestein and rich of proteins. The genestein of soybean milk potentially has antitumor/anticancer, antivirus, antiallergic activities and immunity responses. The aim of this research was to evaluate the immunostimulant activity of soybean milk against immunoglobulin (IgG and IgA) and lymphocyte cells proliferation in Balb/c mice induced by hepatitis A. The test was done at 3 groups of 5 Balb/c mice. Each group was consummed orally with soybean milk(group I) dose of 0.7mL/20g/BW, levamisole (group II, as positif control) dose of 0,45 mg/0.7mL/20g/BW, and water (group III, as negative control) dose of 0.7mL/20g/BW, once a day, through out of the research. On the day of 7, 28 and boostered on the day of 43, all groups were induced intra peritoneally by hepatitis A dose of 5.24 IU/20g/BW. The serum were collected from plexus retroorbitalis by heparinized cappilary on the day of 14, 35 and 46, for IgG and IgA measured by ELISA method, and then the mice were sacrificed to isolate the lymphocytes of spleen. The lymphocyte cells proliferation measured by MTT-reduction method. The result shown that IgG and IgA increased significantly (p<0,05) against levamisole and water at the day-46, but did not increase significantly (p>0,05) against lymphocyte cells proliferation concerning with soybean milk consumed. It can be concluded that the immunostimulatory activity soybean milk was against humoral immunity, instead of seluler immunity.Key words : soybean milk, immunoglobulin, lymphocyte cells, hepatitis A, levamisole
Co-Authors A.A. Ketut Agung Cahyawan W Agilina, Alvani Renata Agustia Damayanti Agustia Damayanti Agustia Damayanti Agustia Damayanti, Agustia Alvani Renata Agilina Anas Putri Maria Natasya Panamuan Apridamayanti, Pratiwi Asri Adyttia Asri Adyttia Asri Adyttia Asri Adyttia, Asri Ayu Pratiwi Butarbutar, Ruth Haryati Ediati Sasmito Egida Rachmadani Gamayanti, Tasiana Gisela Hadi Kurniawan, Hadi Hati, Anita Kumala Herlina, Tutun Intan Khairunnisa Irene Mahwal Khairunnisa, Intan Kusuma, Ria Liberti, Oktaviona Winda Meilita, Dewi Melda Mery Andiriyani Monika Rosmimi Muhammad Akib Yuswar Muhammad Akib Yuswar Muhammad Akib Yuswar Muhammad Akib Yuswar Nabilla Putri Pratiwi Nanang Munif Yasin Nera Umilia Purwanti, Nera Umilia Niken Larasati Novi Permata Sari Nurmainah Nurmainah Nurul Mar&#039;atus Sholihah Octaviani, Peppy Oskar Skarayadi, Oskar Pinasti Utami, Pinasti pramestutie, hananditia rachma Pratiwi Apridamayanti Pratiwi Apridamayanti, Pratiwi Pratiwi, Nabilla Putri Rachmadani, Egida Rafika Sari Rafika Sari Rafika Sari Ratna Widyaningrum Ria Kusuma Rise Desnita, Rise Rizka Humardewayanti Asdie Robiyanto Robiyanto, Robiyanto Robiyanto, Robiyanto Rosmimi, Monika Ruth Haryanti Butarbutar Safitri Caesaria Samudra, Nizam Elang Sholihah, Nurul Mar'atus Shoma Rizkifani Siti Nani Nurbaeti Sri Amalia Sri Amalia Sri Amalia, Sri Sri Mulyaningsih Sri Wahdaningsih Sri Wardaningsih Susanti, Ressi Susanti, Ressi Tri Murti Andayani Vanie, Vanie Veronika, Vina Wirna Maya Sari Wirna Maya Sari Wirna Maya Sari Wirna Maya Sari, Wirna Maya Yunita Fauziah Yunita Fauziah Yunita Fauziah Yunita Fauziah, Yunita Yuswar, M. Akib Yuswar, Muhammad Akib