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Antibacterial Effect of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 ON Klebsiella pneumoniae Producting Extended-Spectrum β-Lactamase (ESBL) Setiawati, Yuani; Retnowati, Wiwin; Basori, Achmad
Jurnal Medis Islam Internasional Vol 1 No 1 (2019): December
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/iimj.v1i1.1326

Abstract

Klebsiella pneumoniae as an opportunistic pathogen can cause nosocomial infection. The main concern on this bacterium is directed on the extended-spectrum β-Lactamase (ESBL)-producing Klebsiella pneumoniae. The therapy of ESBL-producing Klebsiella pneumoniae infections is very limited because of its multidrug resistance. It had been found new local isolates Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 in mangrove East Coast of Surabaya. These isolates have potential to produce antibiotics (Retnowati, 2008). This study was aimed to prove these isolates may inhibit the growth of ESBL-producing Klebsiella pneumonia. The test of antibacterial activity of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 using the modification Agar print method against clinical isolates of ESBL-producing Klebsiella pneumoniae. The diameter of inhibition zone (mm) formed shows activity of these isolates. The profiles of antibacterial activity of Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 were different in terms of periode required to reach peak activity, duration of activity and inhibition zone diameter produced during 10 days of observation. Streptomyces sp-MWS3 reached the peak activity most rapidly on day 3 with the largest inhibition zone diameter of 9 mm in ESBL-producing Klebsiella pneumoniae. There were significant differences in inhibition zone diameter between Streptomyces sp-MWS1, Streptomyces sp-MWS3, and Streptomyces sp-MWS6 against ESBL-producing Klebsiella pneumoniae.
The Use of Prophylactic Antibiotics on Orthopaedic Procedures in an Academic Hospital in Indonesia Setiawati, Yuani; Farhadi, Azmi
Jurnal Medis Islam Internasional Vol 2 No 2 (2021): June
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/iimj.v2i2.2155

Abstract

Background: Surgical site infections (SSIs) are common surgical complications that will increase cost of treatment. The incidence of SSI can be prevented with antibiotic prophylactic. Uncompliance using of prophylactic antibiotics is one of the factors leading to the occurrence a microbes resistance. The data on the using of prophylactic antibiotics in Indonesia is still limited. Objective: This study aims to analyze the using of prophylactic antibiotic guideline on orthopaedic surgery. Methods: The study was conducted retrospectively using data from medical records on patients who had clean and clean-contaminated orthopedic procedures from 2013 to 2016 in the standard operating room of Dr. Soetomo hospital Surabaya. We analyzed the use of prophylactic antibiotics in terms of antibiotic selection, timing of administration, and the compliance to the prophylactic antibiotic local guidelines on orthopaedic surgery. Results: Overall, patient data from 2013 to 2016 was 5246 patients. The compliance rate of prophylactic antibiotics from 2013 to 2016 was 48.3%. This level of compliance uses a selection of antibiotics, dose of administration, delivery mode, delivery time, and route of administration. Conclusion: The results of this study have shown that the prophylactic antibiotic compliance rate on orthophaedic procedures in Soetomo Hospital from 2013 to 2016 was 48,3%. Antibiotic resistance control program quite effective at increasing compliance with the use of the prophylaxis antibiotics.
Effects of Clove Leaf Essential Oil (Syzygium aromaticum) in Inhibiting Biofilm Formation on Candida albicans Isolate Rifai, Achmad; Endraswari, Pepy Dwi; Setiawati, Yuani; Koendhori, Eko Budi
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 15 No. 2 (2024): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V15I22024.70-75

Abstract

Highlights: Candida albicans is capable of forming biofilm, which can lead to resistance to antifungal treatments. Clove leaf essential oil, which can inhibit biofilm formation, making it a potential anti-biofilm agent.   Abstract Introduction: Candida albicans (C. albicans) has a virulence factor, like biofilm formation. Biofilm is a three-dimensional structure that plays a role in antimicrobial resistance, thus requiring antibiofilm agents to address this problem. One of them is Clove leaf (S. aromaticum). S. aromaticum has active phytochemicals (eugenol, β-caryophyllene, and others) that can inhibit biofilm formation in microorganisms, including fungi. This study aimed to prove the effect and to find a concentration of Clove leaf essential oil that affects the biofilm formation of C. albicans isolate. Methods: This study used a microtiter plate with a two-fold dilution technique. The tested concentrations were 6.25%, 3.125%, and 1.5625%. The positive control was 200 µL of C. albicans biofilm suspension, and the negative control was 200 µL of tryptic soy broth media, and all were repeated four times. The biofilm was observed using crystal violet and evaluated using optical density. The data was analyzed statistically using the International Business Machines Corporation (IBM) Statistical Package for the Social Sciences (SPSS) version 26, with a p<0.05 considered statistically significant. Results: The Optical density (O.D.) of the isolate was 2.039, while the negative control was 0.349, indicating that the isolate was a strong biofilm former. The concentrations of 6.25%, 3.125%, and 1.5625% showed inhibition percentages of 8.533%, 17.214%, and 8.484%, respectively. The Optical density was found to be normally distributed but not homogeneous p>0.05, The Kruskal-Wallis’s test was significant, and the Mann-Whitney test was not significant between test groups and positive control. Conclusion: Clove leaf essential oil has inhibitory effects on C. albicans biofilm isolates. However, statistically, there was no significant difference between the test groups and the positive control.
UTILIZATION OF HERBAL POWDER TO INCREASE THE ECONOMY IN NURUL HUDA ISLAMIC BOARDING SCHOOL, SIDOARJO Setiawati, Yuani; kurnijasanti, rochmah; Sudjarwo, Sri Agus; Fitriati, Mariza
Jurnal Layanan Masyarakat (Journal of Public Services) Vol. 8 No. 3 (2024): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v8i3.2024.381-387

Abstract

In the context of global economic contestation, the presence of an independent Islamic boarding school economic community will become social capital and inspiration for the community so that the local community's economy is not defeated and displaced by global players. The Nurul Huda Islamic boarding school is included in the category of Islamic boarding schools that do not yet have a business unit, even though the Nurul Huda Islamic boarding school has vacant land that can be utilized by students by managing herbal plants. Implementation of the community service program is carried out through several activities: 1) Providing material about various kinds of herbs and their benefits 2) Demonstration and practice of formulating and making various kinds of herbal powders 3) Demonstration and practice of formulating and making various kinds of herbal drinks 4) Demonstration and practice of formulation and making herbal granules 5) Demonstration, practice of formulation and making herbal effervescent powder 6) Packing and labelling practices for marketing 7) Making a website for product marketing. This community service produces output in the form of: 1) Publication in the Sinta 4 journal, namely the Abdinus journal / Journal of Indonesian Service 2) Publication on online news media. The results of implementing this activity were able to increase the knowledge and skills of students at Nurul Huda Islamic boarding school.
Stop Bullying Counseling as an Effort to Prevent Bullying Behavior that Occurs in Students at Ketabang Seruni Elementary School Surabaya Fatimah, Nurmawati; Khairul Rizki Purba, Abdul; Fithriyah, Izzatul; Purwaningsih, Sri; Setiawati, Yuani; Khaerunnisa, Siti; Akbar Kurniawan, Mohammad
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 9 No 3 (2024): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The phenomenon of bullying is a serious problem for child development in Indonesia. Bullying cases in Indonesia are ranked fifth in Indonesia world in bullying incidents (Jayani, 2019). Violence against students at school is a topic of discussion mainstream in the media. The incidence of bullying is 10-60% of children who receive ridicule, ridicule, expulsion, kicking or pushing at least once a week. Cases of bullying in Children can occur anywhere, anytime and by anyone. This makes this case still high incidence rate (Komisi Perlindungan Anak Indonesia, 2020). If this bullying incident continues for a long time, it will have an impact on the victim of bullying, both physically, psychologically and socially. The bullying case at Ketabang Seruni Elementary School Surabaya is of particular concern to the school principal. During break time, students are often heard making fun of each other, and there are friends who are alone because they are ostracized. As a leading public school in the city of Surabaya, hopes that its students can be a good for other schools by implementing free bullying at schools. The solution offered to solve this problem is to provide education to students at Ketabang Seruni Elementary School Surabaya by providing an explanation first about the purpose of the activity, then giving a questionnaire to the students about various things related to the meaning of bullying, types of bullying, causes of bullying, characteristics of perpetrators and victims of bullying, bad impacts of bullying, prevention of bullying, efforts to handle bullying prevention. Education is provided by providing materials using powerpoint media, leaflets and posters which are pasted on the school walls as a means of reminder for the entire community at school, including students, teachers, employees and parents. The result of this activity is that there is an increase in students' understanding of bullying and good practices are obtained by changing the way students socialize to be better and more polite. Keywords — Mental health; Education; Discrimination; Dignity
Antibacterial Activity Test of Green Betel Leaf Extract (Piper betle L.) Against Methicillin-Sensitive Staphylococcus aureus Manaf, Syifa Az Zahrah; Wiqoyah, Nurul; Setiawati, Yuani
Jurnal Ilmu Kesehatan dan Kesehatan Vol 9 No 1 (2025): FEBRUARY : Fragmentation In Modern Healthcare Approaches Amid Rising Chronic And
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v9i1.6521

Abstract

Background: Asia is one of the regions with the highest prevalence rates of Staphylococcus aureus infection. Green betel leaves (Piper betle L.) have been proven to have antibacterial potential with their active compound content, namely essential oils, phenols, chavicol, flavonoids, alkaloids, saponins, tannins, and steroids so it is necessary to investigate whether betel leaf extract has an effect on Methicillin-Sensitive Staphylococcus aureus (MSSA). The aim of this study is to analyze the effect of green betel leaf extract on the growth of MSSA.  Methods: This research is a descriptive experimental study regarding the antibacterial activity test of green betel leaf extract against MSSA. The MSSA bacteria used were bacterial preparations in the Microbiology Laboratory of Faculty of Medicine Airlangga University. Green betel leaf extract was obtained from Batu City in 2023. The antibacterial activity test technique used was the dilution test. Results: In the dilution test, for the growth of MSSA, in tubes with concentrations of 800 mg/mL, 600 mg/mL, 400 mg/mL, 200 mg/mL, and 100 mg/mL appear clear indicates that there is no growth of germs. At a concentration of 50 mg/mL it appears cloudy which indicates the growth of germs. The results obtained for the growth of MSSA on agar plates were at concentrations of 800 mg/mL, 600 mg/mL, 400 mg/mL, and 200 mg/mL, there was no growth of germs. Meanwhile, at concentrations of 100 mg/mL and 50 mg/mL, the growth of MSSA germs was found Conclusions: The conclusion of this research is that the minimum inhibitory concentration (MIC) of green betel leaf extract against MSSA is 100 mg/mL and the minimum bactericidal concentration (MBC) of green betel leaf extract against MSSA is 200 mg/mL. Further research is needed on the antibacterial activity of green betel leaf extract against other bacterial species that are resistant to many drugs.
Antibacterial Activity Test of Green Betel Leaf Extract (Piper betle Linn.) against Methicillin-Sensitive Staphylococcus aureus Manaf, Syifa Az Zahrah; Wiqoyah, Nurul; Setiawati, Yuani
Majalah Biomorfologi Vol. 35 No. 2 (2025): Majalah Biomorfologi
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v35i2.2025.131-139

Abstract

Highlights Green betel leaf extract, particularly cultivated in Batu City, can inhibit and kill MSSA. Green betel leaf extract, particularly cultivated in Batu City, can be developed into a new alternative treatment as an effective antibiotic to kill MSSA bacteria.   ABSTRACT Background: Asia is one of the regions with the highest prevalence rates of Staphylococcus aureus (S. aureus) infection. Green betel leaves (Piper betle Linn.) have been shown to possess antibacterial potential due to their active compound content. Objective: To investigate whether betel leaf extract affects methicillin-sensitive Staphylococcus aureus (MSSA). Material and Method: This research was a descriptive experimental study focusing on the antibacterial activity test of green betel leaf extract against MSSA. The MSSA bacteria used were bacterial preparations in the Microbiology Laboratory of the Faculty of Medicine, Universitas Airlangga. The green betel leaf extract was obtained in 2023 in Batu City, East Java, Indonesia. The antibacterial activity test technique used was the dilution test. The tool used in data processing was Microsoft Excel. Results: Germs were found in the dilution test, particularly in tubes with concentrations of 50 mg/mL. The study also highlighted that no growth of germs was found based on the results obtained for the increase of MSSA on agar plates at concentrations of 800 mg/mL, 600 mg/mL, 400 mg/mL, and 200 mg/mL. The study also found that the growth of MSSA germs was at 100 mg/mL and 50 mg/mL. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of green betel leaf extract against MSSA were 100 mg/mL and 200 mg/mL, respectively. Conclusion: The extract of green betel leaves, particularly cultivated in Batu City, East Java, Indonesia has been shown to possess antibacterial activity, which can inhibit and kill MSSA bacteria. Further research is needed on the antibacterial activity of green betel leaf extract against other bacterial species.
Mental Health Status and Literacy of Adolescent in Rural Area of Mojokerto, East Java, Indonesia Ardana, Arin Aurilia; d'Arqom, Annette; Puteri, Fira Adilah; Indiastuti, Danti Nur; Nasution, Mhd Zamal; Hasanatuludhhiyah, Nurina; Qorib, Mohammad Fathul; Setiawati, Yuani; Junaidah Yusof
Jurnal Psikiatri Surabaya Vol. 14 No. 2 (2025): November
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jps.v14i2.64237

Abstract

Introduction: Adolescence is a crucial period for the development of mental health. Enhancing mental health literacy can reduce stigma and support early intervention and treatment. This study aims to examine the mental health status and mental health literacy among middle and late adolescents in a rural area of Mojokerto District, East Java, Indonesia. Methods: A total of 81 high school students from Mojokerto District participated in the study. The Depression Anxiety Stress Scale – Youth version (DASS-Y) was used to assess symptoms of depression, anxiety, and stress. Mental health literacy was measured using the Adolescent Mental Health Literacy Questionnaire (AMHL). The validity of the AMHL was tested on 30 students using Pearson correlation. Descriptive statistics were used to analyze the prevalence of mental health conditions and levels of mental health literacy. Inferential statistics were applied to explore associations between mental health status, literacy, and sociodemographic variables. Results: The results showed that 53% of students experienced symptoms of mental health problems. While most students demonstrated moderate to satisfactory mental health literacy, significant gaps remain. Enhancing literacy may help reduce stigma and improve knowledge and attitudes toward mental health. Sociodemographic factors and mental health literacy showed limited influence on mental health status. However, sex was significantly associated with both mental health literacy and mental health status (p = 0.016 and p = 0.032, respectively). Conclusion: Mental health during adolescence significantly influences adult well-being. Improving adolescent mental health literacy is crucial for early recognition, stigma reduction, and better access to mental health care.
Aktivitas Antibakteri Ekstrak Etanol Daun Lamtoro (Leucaena Leucocephala) Terhadap Staphylococcus Aureus dengan Metode Dilusi Al Alim, Muhammad Khafidin; Purwanta, Marijam; Setiawati, Yuani
Cerdika: Jurnal Ilmiah Indonesia Vol. 2 No. 2 (2022): Cerdika: Jurnal Ilmiah Indonesia
Publisher : Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/cerdika.v2i2.345

Abstract

Latar Belakang: Lamtoro (Leucaena leucocephala) merupakan tumbuhan yang umum di indonesia. Tanaman ini selain bisa dimanfaatkan sebagai bahan pangan, daunnya juga sering dipakai masyarakat indonesia sebagai obat luka atau memar. Salah satu bakteri yang umumnya terdapat dalam luka adalah Staphylococcus aureus. Oleh karena itu, peneliti menduga bahwa ekstrak daun lamtoro memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Tujuan: Penelitian ini dilakukan untuk menguji aktivitas antibakteri daun lamtoro terhadap Staphylococcus aureus dengan metode dilusi. Metode: Ekstrak kental lamtoro dibuatkan seri pengenceran pada medium cair. Bakteri uji kemudian ditambahkan dengan proporsi yang sama pada setiap tabung. Tabung dengan konsentrasi ekstrak terendah yang masih terlihat jernih ditetapkan sebagai KHM. Selanjutnya dilakukan streaking pada media biakan. Setelah inkubasi, media dengan konsentrasi terendah yang tidak didapati pertumbuhan koloni ditetapkan sebagai KBM. Hasil: Kekeruhan yang disebabkan ekstrak tidak dapat dibedakan dengan kekeruhan yang disebabkan bakteri, sehingga KHM tidak dapat ditentukan. Dari hasil kultur, didapatkan pertumbuhan koloni pada seluruh media biakan, sehingga KBM juga tidak dapat ditentukan. Hasil hitung koloni menghasilkan bahwa ekstrak tidak membunuh bakteri namun masih dapat menghambat pertumbuhan bakteri tersebut paling optimal pada konsentrasi 125 mg/mL dan 62,5 mg/mL. Kesimpulan: KHM dan KBM ekstrak daun lamtoro terhadap Staphylococcus aureus pada penelitian ini tidak dapat ditentukan. Ekstrak lamtoro tidak membunuh Staphylococcus aureus, namun masih dapat menghambat pertumbuhan bakteri tersebut, paling optimal pada konsentrasi 125 mg/mL dan 62,5 mg/mL.
The Protective Effect of Amorphophallus oncophyllus against Indomethacin-Induced Duodenal Mucosal Injury in Rats via Inhibition of Inflammatory Cell Infiltration Setiawati, Yuani; Kurnijasanti, Rochmah; D'Arqom, Annette; Nugraha, Jusak; Illiandri, Oski; Sudjarwo, Sri Agus
Borneo Journal of Pharmacy Vol. 8 No. 4 (2025): Borneo Journal of Pharmacy
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjop.v8i4.10233

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) can cause significant gastrointestinal mucosal injury, particularly in the duodenum. This damage is often initiated by localized erosion of the duodenal mucosa, which triggers a subsequent inflammatory cascade. The porang tuber (Amorphophallus oncophyllus) is a promising candidate for mitigating this injury due to its rich composition of bioactive compounds, including flavonoids, alkaloids, tannins, and glucomannan, all of which possess known anti-inflammatory properties. This study investigated the protective effect of an ethanolic A. oncophyllus extract on the duodenal mucosa in an indomethacin-induced rat model. Thirty Wistar white rats (Rattus norvegicus) were randomly assigned to five groups: a vehicle control, a model group receiving indomethacin, and three treatment groups that were pretreated with A. oncophyllus extract at doses of 50, 100, and 200 mg/kg, respectively, for seven days before a single dose of indomethacin. Our findings demonstrated that the A. oncophyllus extract, particularly at a dose of 200 mg/kg, effectively reduced duodenal mucosal injury caused by indomethacin. This protective effect is likely attributable to the extract's phytochemical profile, which contains flavonoids, tannins, saponins, terpenoids, and alkaloids. In conclusion, this research confirms that A. oncophyllus extract has the potential to protect the duodenal mucosa from NSAID-induced damage.
Co-Authors Abdul Khairul Rizki Purba Mustofa Indwiani Astuti Achmad Basori, Achmad ACHMAD RIFAI Agung Dwi Wahyu Widodo Akbar Kurniawan, Mohammad Al Alim, Muhammad Khafidin Anna Surgean Veterini Annete d’Arqom Annette d’Arqom, Annette Ardana, Arin Aurilia Arifa Mustika Asrianti, Nur Ilma Awaluddin, Rizki Baitul Fatimah Benny Iswanto Pantoro Cindy ApriliaEkaPrasanty Dennia Oktavia Zahidah Hulwah Dennia Oktavia Zahidah Hulwah Dewangga Sakti Satria Kinasih Dewinta Enggar Pramesthi Djoko Marsudi Eko Budi Koendhori, Eko Budi Fadilah Fadilah Farhadi, Azmi Farhan Nurdiansyah Fatimah, Nurmawati Felicia Jiwantono Feriawan Tan Fiqih Faizara Ustadi Firda Fitra Qolbina Fitriati, Mariza Gatot Soegiarto Indiastuti, Danti Nur Indri Safitri Mukono, Indri Safitri Isyroqiyyah, Nur Moya Izzatul Fithriyah Jihan Bobsaid Jihan Bobsaid Junaidah Yusof Jusak Nugraha Khaerunnisa, Siti Kusuma Eko Purwantari Maftuchah Maftuchah Manaf, Syifa Az Zahrah Maya Hapsari Kusumaningtyas Mhd Zamal Nasution Mia Rahardjo Mohammad Fathul Qorib Muflikhah Ramadhani Muflikhah Ramadhani Muhammad Khafidin Al Alim NOOR CHOLIES Nurina Hasanatuludhhiyah Nurul Wiqoyah, Nurul Oski Illiandri Pepy Dwi Endraswari, Pepy Dwi Purwanta, Marijam Puteri, Fira Adilah Raden Mohamad Herdian Bhakti Rehatta, Nancy Margarita Rochmah Kurnijasanti Rochmah Kurnijasanti Safira Salsabila Citagami Shazia Hafazhah Aulia Siti Khaerunnisa Siti Khaerunnisa Sri Purwaningsih Trisniartami Setyaningrum Vivi Permata Sari Wiwin Retnowati Wiyogo, Illona Okvita Yuri Widia, Yuri