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All Journal Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis Jurnal Pengabdian Magister Pendidikan IPA Jurnal Pengabdian Masyarakat Sains Indonesia (Indonesian Journal Of Science Community Services) Jurnal Ilmiah Profesi Pendidikan Journal of Classroom Action Research NUSRA: Jurnal Penelitian dan Ilmu Pendidikan Jurnal Pijar MIPA Jurnal Pendidikan dan Pengabdian Masyarakat
Lalu Zulkifli
Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Physics Public Health Social Sciences Other

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INDUKSI KALUS KACANG TANAH (Arachis hypogaea L.) VARIETAS KELINCI DENGAN PERLAKUAN 2,4-D DAN BAP Ida Royani; Lalu Zulkifli; Prapti Sedijani
Jurnal Penelitian Pendidikan IPA Vol. 1 No. 2 (2015): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v1i2.21

Abstract

Callus induction determines the successfulness of micropropagation through somatic embryogenesis. This research was conducted to determine the effect of 2,4-D and BAP on callus induction of peanut (Kelinci strain). Callus induction was done on MS medium, with the observed variables were of initiation time, diameter, texture and color callus. Statistical analysis showed that 2,4-D and BAP affect the initiation time, diameter, texture and color callus. The earliest initiation of callus formation was obtained from MS medium with the addition of 4 mg/L 2,4-D. The diameter of the largest callus obtained from MS medium with the addition of 3 mg/L 2,4-D + 1 mg/L BAP. Callus color were affected by the treatment of 2,4-D and BAP, while callus texture was not affected by these treatmen, in which compact callus texture was not affected by these treatment, in which compact callus texture was observed in all treatments.Keywords: Induction of callus, 2,4-D, BAP, peanuts
ANALISIS KEMAMPUAN PENDIDIK DALAM MENERAPKAN PENILAIAN AUTENTIK PADA MATA PELAJARAN BIOLOGI KELAS X IPA SMA DI KABUPATEN LOMBOK TIMUR LL. Zainal Haqiqi; Agus Ramdani; Lalu Zulkifli
Jurnal Penelitian Pendidikan IPA Vol. 4 No. 1 (2018): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v4i1.60

Abstract

This study aims to know the abilities of the teachers in applying authentical assessment in biology at grade X IPA Senior High School of Lombok Timur district. This study using descriptive method which getting data in the verbal only not in numbers. Getting samples at 8 Senior High Schools of Lombok Timur district which aplicate kurikulum 2013 they are SMAN 1 Masbagik, SMAN 2 Aikmel, SMAN 1 Sikur, SMAN 1 Terara, SMAN 2 Selong, SMAN 1 Keruak, SMAN 1 Montong Gading and SMAN 1 Sakra. To these teachers of biology were given instruments which asked how the authentical assessment reseach done in the schools and the result were presented in this study. The ability of teachers in applying authentical assessment were different from every questions there are attitude, knowledge and skill. Attitude they are observations, self assessment, assessment among students, journal and interview were answered by teachers with yes 65,62% , knowledge were answered by teachers by yes 88,75% and skill were answered yes 87,75%. Ability of teachers in applying authentical assessment were answered yes as many as 78,75% and the next verbally answered by teachers because of the problems in applying authentical assessments. Constraintburden of teachers in design and applying authentical assessment were given 5 questions and the interview of the execution of authentical assessments were given about 91 questionsAutentical assessment, biology
BAKTERI ENDOFIT KULIT BATANG TANAMAN CENGKEH (Syzygium aromaticum L.) DAN KEMAMPUANNYA SEBAGAI ANTIBAKTERI Citra Sepriana; Dwi Soelistya Dyah Jekti; Lalu Zulkifli
Jurnal Penelitian Pendidikan IPA Vol. 3 No. 2 (2017): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v3i2.92

Abstract

This research was conducted to find out the capabilities of endophytic bacteria isolated from bark of the clove plants in inhibiting the growth of bacteria Streptococcus mutans, Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli and to identify endophytic bacteria that potensial to produce an antibacterial. Stages of this research include the isolation of endophytic bacteria, antibacterial test, biochemical test, and molecular identification based on 16 S rRNA. It was obtained about 29 isolates from bark of the cloves plants in which 6 of them isolates inhibited bacterial S. aureus and 3 isolates inhibited bacterial S. aureus and S. mutans. Based on 16S rRNA molecular identification, isolates of endophytic bacterial of cloves plants bark which have inhibitory is closely related to Bacillus cereus JL and Bacillus amyloliquefasiensKeywords: Bark of the cloves, Endophytic bacterial, Molecular  identification
ISOLASI, KARAKTERISASI DAN IDENTIFIKASI BAKTERI ENDOFIT KULIT BATANG SRIKAYA (ANNONA SQUAMOSA) DAN POTENSINYA SEBAGAI ANTIBAKTERI Lalu Zulkifli; Dwi Soelistya Dyah Jekti; Samsul Bahri
Jurnal Penelitian Pendidikan IPA Vol. 4 No. 1 (2018): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v4i1.98

Abstract

The objective of this study was to isolate endophytic bacteria from bark of srikaya, analyzing antibacterial activity of endophytic bacteria in S. aureus, B. cereus and E. coli pathogenic bacteria, characterizing endophytic bacteria capable of inhibiting the growth of pathogenic bacteria and identification of endophytic bacteria able to inhibit the growth of pathogenic bacteria. Isolation of endophytic bacteria using TSA and NA media, bioassay on pathogen bacteria with concentration of 106 cells / ml with using Ø 6 mm wells and entering supernatant of 100 μl. Supernatant was obtained by growing endophytic bacteria in NB media shaken with shaker 150 cycles / min for 48 h at 32ᵒC then culture centrifuge at 5000 g for 30 min. Positive control using cyprofloxacin. Characterization is based on the nature of the colony, Gram paint, spore formation, and biochemical tests. The results of the study yielded 13 endophytic bacterial isolates and 4 endophytic isolates capable of inhibiting the growth of 8 pathogenic bacteria with sensitive criteria, 2 pathogenic bacteria with resistant criteria and 1 pathogen bacteria can not be inhibited its growth. Gram's paint results show that 4 endophytic isolates belong to Gram-positive, rod-shaped and spore forming cells. From the character possessed by the bacteria can be identified that the 4 bacteria endofit capable of inhibiting pathogenic bacteria are Bacillus brevis, Bacillus latesporus, Virgibacillus pantothenticus, and Bacillus circulansKeywords: Characterization, Endophytic Bacteria, Bark of Srikaya, Antibacterial
BAKTERI ENDOFIT KULIT BATANG TERAP (ARTOCARPUS ELASTICUS) DAN AKTIFITASNYA SEBAGAI ANTIBAKTERI Guplin -; Dwi Soelistya Dyah Jekti; Lalu Zulkifli
Jurnal Penelitian Pendidikan IPA Vol. 3 No. 2 (2017): July
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v3i2.106

Abstract

This study aims to identify the types of endophytic bacteria on the bark of terap (Artocarpus elasticus) capable of inhibiting the growth of clinical isolate bacteria of Staphylococcus aureus, Escherichia coli, Salmonella typhi, and Pseudomonas aeruginosa. Stages of this study were (1) isolating the endophytic bacteria from the bark of terap plants (2) antibacterial test (inhibition test) against clinical isolate bacteria, (3) biochemical test upon endophytic isolate bacteria showing strong  inhibition effect, (4) moleculer identifcation upon endophytic bacteria isolate showing strong  inhibition effect. From this study, it was obtained 34 barks of terap isolates, and 12 isolates have inhibitory effect on S. aureus. Based on 16S rRNA   molecular identification, the isolate of endophytic bacteria of the barks were identified as Bacillus cereus, Bacillus subtilis and Bacillus amyloliquefaciens. Those  endophytic bacteria of Terap plants  are potentially  to be  developed as a the producer of antibacterial compounds.Keywords: Terap bark, Endophytic bacteria, Identification, Antibacteria
Antimicrobial Activity of Pome granataum’s Endophytic Bacteria Against Pathogenic Microbe Putri Alfianti Sriwinahyu; Prapti Sedijani; Lalu Zulkifli
Jurnal Biologi Tropis Vol. 20 No. 3 (2020): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v20i3.2099

Abstract

White pomegranate (Punica granatum L.) is one of potential plant that can be use as an antimicrobial. The objective of this study was to determine antimicrobial activity of endophytic bacteria from white pomegranate (Punica granatum L.) in inhibiting the growth of pathogenic microbia (Staphylococcus aureus, Streptococcus viridans, Shigella dysentriae, Klebsiella pneumoniae and Fusarium sp. fungi). The stages of the research were isolation endophytic bacteria, antimicrobial assay, and characterization of potential endophytic bacterial. Endophytic bacterial were isolated from bark, leaves, flower, and fruit peel of white pomegranate taken from Gebang Baru, Mataram District. Antimicrobial assay was then performed using agar diffusion method, using supernatant of endophytic bacteria, water as negative control or ciprofloxacin as positive control. The potential isolates were characterized using Gram staining and biochemical test. The results showed that 2 endophytic bacterial isolates (DNX2 and BNG1) show strong activity against S. aureus, 3 endophytic bacterial isolates (BTG1, DNX11, and BNG1) show strong activity against S dysentriae, and 1 isolate KLBX11 shows medium activity against Fusarium sp. fungi. Those isolates are gram-possitive with bacill-shaped and produce spores. It can be concluded that it is possible to obtain potential endophytic bacterial from white pomegranate are potential as an alternative source for antimicrobial compounds.
Microalgae Diversity as a Bioindicator of Water Quality in Batujai Dam, Central Lombok Hairunnadawiah Hairunnadawiah; Khairuddin Khairuddin; Lalu Zulkifli
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.3084

Abstract

Batujai Dam is one of the largest dams in Central Lombok with an area of 890 ha of inundated water. The water from the Batujai Dam is used for irrigation of agricultural land, flood control, freshwater fisheries and the development of micro-hydro power plants. Batujai Dam is also a reservoir for several rivers in Central Lombok, namely:  the Leneng River, the Sade River and the Dodokan River. This function makes the Batujai Dam a place for the accumulation of various forms of pollutants carried by the inlet rivers. Microalgae is one of the ecological parameters that can function as a bioindicator of water quality. The purpose of this study was to determine the water quality in the Batujai Dam in terms of the microalgae diversity index and the measurement of environmental factors. Determination of sampling point and sampling time using purposive sampling method. Measurement of water quality is carried out by determining the diversity index and measuring environmental factors such as pH, temperature, DO, BOD, phosphate and nitrate. Microalgae data for determining the diversity index was analyzed using the Microsoft Excel 2010 program. The results showed that the identified microalgae consisted of 5 classes, 16 orders and 40 species. The overall diversity index of microalgae in the Batujai dam is 1.712 with a DO value of 5.09 and a BOD of 4.0175. Based on these data, it can be concluded that the water quality in the Batujai Dam is in an unhealthy condition (lightly polluted). The allotment for the use of water from the Batujai Dam is suitable for freshwater fish farming, livestock and agricultural irrigation, and it is not recommended for drinking water sources and water recreation infrastructure/facilities.
Isolation, Purification, and Toxicity Test of Bacillus thuringiensis from Cows Cage Soil Againts Drosophila melanogaster Syamsul Bahri; Lalu Zulkifli; Dewa Ayu Citra Rasmi; Prapti Sedijani
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.3221

Abstract

Bacillus thuringiensis is one of the bacteriae species that be able to kill insects. The latest research showed that the cell plasm of this bacteria contains crystalline protein with natural insectisides properties. Therefore the insecticidal effect of this species on insects which is decreasing cultivation plant production is interest to be examined. One of the insect species that are well-known causes of loss of farm production in fruit flies (Drosophila melanogaster). Therefore this research is designed to isolate, purify, and thenassess Bacillus thuringiensis toxicity against Drosophila melanogaster as animal treatment. Bacillus thuringiensis was successfully isolated from soil of  cows cage by using Lauria Bertani medium contain 0,25 M buffer solution Acetic, pH 6.8. The result shows that Bacillus thuringiensis isolate is a gram-positive bacteria. Position of endospore is located between the middle and the end of the cell (subterminal spore). Toxicity test was conducted by mixing 0.5 g/L, 1 g/L, 2 g/L, 4 g/L of pure Bacillus thuringiensis isolate into  Drosophila melanogaster medium. The treatments effect were monitored for 15 consecutive days. Toxicity test shows that the treatment has no significant effect on the treated animal mortality. This result shows that crystal-producing Bacillus thuringiensis strain isolated from cows cage soil do not have insecticidal properties for Drosophila melanogaster. It is concluded that Drosophila melanogaster is a non-target species of Bacillus thuringiensis.
Community Structure of Bacillariophyceae in the Water of Klui Beach, North Lombok Yuni Safrian Hadi; Lalu Japa; Lalu Zulkifli
Jurnal Biologi Tropis Vol. 22 No. 2 (2022): April - June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i2.3398

Abstract

Coastal areas have potential that can be developed by the community, one of which is beach tourism. Tourism activities around that take place around the coast can affect the aquatic ecosystems especially the community structure of phytoplankton. Phytoplankton are aquatic organisms that act as primary producers. Bacillariophyceae is a class of phytoplankton that is commonly abundant in marine waters. The objectives of this study were to determine the species composition, species abundance, and community structure of Bacillariophyceae in the waters of Klui Beach. This type of research is descriptive-explorative. The Haphazard sampling was applied for determining the sampling sites. Phytoplankton data were analyzed using the abundance formula, diversity index, uniformity index, and dominance index. The results showed that the composition species of Bacilllariophyceae consisted of 48 species which included in 17 orders. Average species abundance was 298.09 ind/L (low category). The spesies diversity index was 2.37 (moderate category), the species uniformity index was 0.86 (high category), and the species dominance index was 0.13 (low category).
The effect of Lemongrass (Cymbopogon nardus) Extract in inhibiting Bread Fungal Growth, Aspergilus Oyizae Using a combination of N-Hexane-Ethanol Solvent Priyo Hartanto; Prapti Sedijani; Lalu Zulkifli; Miko Erniarti
Jurnal Biologi Tropis Vol. 22 No. 1 (2022): January - March
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v22i1.3736

Abstract

The purpose of this study was to determine the effect of lemongrass extract (Cymbopogon nardus) in inhibiting the growth of the bread fungus Aspergillus oryzae by using a combination of lemongrass extract with n-hexane as a solvent. This type of research is experimental research. The population in this study were all types of mushrooms isolated from bread. The sample used was the fungus Aspergillus oryzae isolated from bread. Lemongrass extraction was carried out by maceration method with n-hexane and ethanol as solvents separately, followed by mixing the two extracts with a combination of n-hexane extract: ethanol extract respectively as follows: 1:1; 2:1 and 1:2 before evaporation. The inhibition test of lemongrass extract against the fungus Aspergillus oryzae was carried out by growing the fungus on PDA media which had been supplemented with lemongrass extract with a concentration of 0.6%; 0.8% and 1%. Data were analyzed using the ANOVA test at a significance level of 95%, followed by the Honest Significant Difference (BNJ) test. The results showed that the combination of n-hexane extract-ethanol extract 1:1 and 1:2 showed low inhibition at all concentrations tested, while the combination 1:2 with 1% lemongrass extract concentration was the best combination in inhibiting the growth of the fungus Aspergillus oryzae ( 1/4 control mycelium colony diameter) and showed 74% inhibitory activity (strong inhibitory category). From the research, it was concluded that lemongrass extract using a combination of ethanol solvents, N-Hexane extract - ethanol extract with a 1:2 combination applied with a concentration of 1% inhibited the growth of the fungus Aspergillus oryzae in the category of strong inhibition, with an inhibitory activity of 74%.
Co-Authors A Wahab Jufri A.A. Ketut Agung Cahyawan W AA Sukarso Abdul Syukur Abdul Syukur Abdul Syukur Abdul Syukur Agil Al Idrus Agil Al Idrus Agil Al Idrus Agil Al Idrus Agus Ramdani Agus Ramdani Ahliah Ghurfah Ahmad Raksun Ahmad Raksun Ahmad Raksun Ahmad Raksun Ahmad Raksun, Ahmad Alfina Alfina Aliefman Hakim Anida Firliana Dewi Anindita Suliya Hangesti Mandra Ariana Ashri Dewi Arinal Hidayati Aris Doyan Aris Doyan Asmita Atika Qomaria Ayun Pazira Baiq Endang Kurnia Ramdhani Bq Sari Hidayatullah Dadi Setiadi Dewa Ayu Citra Rasmi Dewa Ayu Citra Rasmi Dewa Ayu Citra Rasmi DIDIK SANTOSO Didik Santoso Dinda Febriani Dinda Nurhandayani Dita Rieza Permatasari Dwi Soelistya Dyah Jekti Dwi Soelistya Dyah Jekti Dyah Rahayu Elma Vanani Emilga Estiana Fadhila Karunia Fitri Fadilla Dwi Pangestuti Gunawan Gunawan Guplin - H. Mahrus Hairunnadawiah Hairunnadawiah Handriani Handriani Hendri Yogi Pratama Hikmawati Hikmawati Husniatul Ummi I Gde Mertha I Gde Mertha I Wayan Mertha Ida Ayu Putu Armyani Ida Royani Ilhamdi, Mohammad Liwa Imam Bachtiar Irawan Zain Irawan Zain Ispaniah Jamaluddin Jamaluddin - Jamaluddin Jamaluddin Jannaturroyani K. Khairuddin K. Kusmiyati Karima Paspania Karnan Karnan Khairuddin Khairuddin Laela Innaza Laela Salsa A Lala Dwi Sasmitha Lalu Habib Lalu Japa Lalu Japa Lalu Muh. Sofyan Hadinata Lalu Ramas Arya Lingga Daniswara LL. Zainal Haqiqi Luthfiyannisa Luthfiyannisa M. Galih Dwi Ariesta M. Irpan Siswandi M. Liwa Ilhamdi M. Yamin Mahrus Mahrus Mahrus Mahrus Mahrus Mahrus Mahrus Mahrus Mahrus Mahrus Maratun Basitha Melia Anggriana Miko Erniarti Mila Aulina Agustin Moh. Liwa Ilhamdi Mohammad Liwa Ilhamdi Mohammad Liwa Ilhamdi Muh. Gibran Abdurrahman Muhammad Fikri Alfiansyah Muhammad Liwa Ilhamdi Muhammad Rainaldy Juliansyah Nada Purnama Sari Nita Setiawati Nova Fitriani Wahdah Nuraeni Muntari Nurfajrin Aliyatri Nurmasita Nurul Indriani Nurul Rizqiyah Prapti Sedijani Prapti Sedijani Prapti Sedijani Priyo Hartanto Putri Ainayah Alfatihah Putri Alfianti Sriwinahyu Raja Akbar Rani Musmiawati Ranti Eka Lestari Rizky Tri Septiawan Sahratullah Samsul Bahri Saprizal Hadisaputra Sekar Partiwi Sepriana, Citra Sri Handayani St. Rahmadani Syafruddin Syafruddin Syahruddin AR Syamsul Bahri Syamsul Bahri X Zardht Alex Hidayat Yeyen Nilamsari Putri Yulia Ningsih Yuni Safrian Hadi Yunita Fatmala Zuaem