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Journal : BERKALA SAINSTEK

Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb Hartanti, Lucky; Ashari, Asri Mulya; Warsidah, Warsidah
BERKALA SAINSTEK Vol 9 No 3 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i3.27179

Abstract

Uncaria gambier Roxb is a plant from the Rubiaceae family, belongs to the Bajakah group and has been used empirically in the treatment of various types of diseases in the community. Besides being one of the industrial plants and export commodities produced in Sumatra and Kalimantan, gambier leaves is also used frequently in Indonesia, both for the pharmaceutical and cosmetic industries. As a result, market demand both nationally and internationally have increased. The aim of this research are determination of antioxidant activity of ethanol extract and aqueous extract of gambir claw using 1,1-diphenil-2-picrylhydrazyl (DPPH) and determination of total phenol using Calcetau folin reagent. Gambier plant samples were taken in the forest area around the Tanjungpura University campus, the claws were separated and dried at room temperature, then powdered and extracted by maceration using 70% ethanol pa and aquadest, then the extract was concentrated until it was ready to be tested for antioxidant activity and determination of total phenol. The total phenolic ethanol extract and aqueous extract of gambir claw were 224.66 mg GAE/g extract (GEA : Gallic Acid Eqivalent) and 299.08 mg GAE/g extract, respectively, while the antioxidant potential (IC50) was 39.566 μg/mL and 65.140 μg/mL, and the IC50 for comparison of Vitamin C was 7.02 μg/mL.
Proximate and Essential Macrominerals Analysis of Tembakul (Mudskipper) Fish Flour as a Food Source for Stunting Prevention Lestari, Desriani; Hartanti, Lucky; Sofiana, Mega Sari Juane; Yuliono, Agus; Kurniadi, Bambang
BERKALA SAINSTEK Vol 10 No 1 (2022)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v10i1.31030

Abstract

The main cause of stunting is due to inadequate food intake according to the needs for growth and development of children, or insufficient nutrition of pregnant women. The low nutritional intake of pregnant and lactating mothers as well as children under five years old who are still growing and developing is generally caused by the wrong diet. One of the efforts to prevent stunting from an early age is to search for several sources of animal source food containing protein, fat and macro essential minerals according to nutritional standards. The purpose of this study was to determine the proximate content including protein and fat nutrition, water content and ash content, as well as mineral content including calcium, iron and phosphorus, from Tembakul (mudskipper) fish flour as the main needs in the growth of children, during pregnancy and lactation. Protein analysis was carried out using the Kjeldahl method, and fat analysis by extraction using a non-polar solvent, while the determination of essential macro minerals calcium and iron was carried out using an Atomic Absorption Spectrophotometer, and phosphorus analysis using ultraviolet spectrophotometry. The proximate content of Tembakul (mudskipper) fish flour from the Mempawah mangrove ecosystem is fat content (1.80%), protein content (50.67%), water content (24.58%), and ash content (20.20%). Mudskipper fish flour contains 3 essential minerals, namely Calcium (2.15%), Phosphorus (2.50%) and Iron (270.26 ppm).