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Identifikasi Ektoparasit pada Benih Ikan Mas (Cyprinus carpio) di Balai Benih Ikan Kabat, Kabupaten Banyuwangi Mohammad Faizal Ulkhaq; Darmawan Setia Budi; Gunanti Mahasri; Kismiyati -
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Diseases caused by parasitic groups get less serious attention from the farmers because it is chronic morbidity and mortality rates and small. However, if observed further, parasitic diseases may trigger secondary infection by bacteria, viruses and fungi that can cause death and high losses. Thus this study aims to identify the types of ectoparasites that infected the seed goldfish in BBI Kabat, Banyuwangi which the results will be used as an early warning to prevent the death of fish in bulk. The research sample in the form of seeds 3-5 cm carp amounted 150 obtained from BBI Kabat, Banyuwangi. Ectoparasites inspection method performed by scraping the surface of the body, fins and gills of fish, and then observed under a microscope with a magnification ranging from 40-400x. The observation of ectoparasites are further identified accordance morphology based on the keys of identification. Identified ectoparasites infect seed goldfish in BBI Kabat, Banyuwangi consists of 8 genus is Zoothamnium sp., Trichodina sp., Oodinium sp., Vorticella sp., Argulus sp., Lernaea sp., Dactylogyrus sp., and Gyrodactylus sp. Further research is needed to determine the prevalence and intensity of parasites and appropriate measures to prevent the spread of parasites in healthy fish in BBI Kabat, Banyuwangi.

EVALUASI PEMBERIAN PROBIOTIK BACILLUS PADA MEDIA PEMELIHARAAN TERHADAP LAJU PERTUMBUHAN DAN PERUBAHAN HISTOPATOLOGI IKAN LELE DUMBO (Clarias gariepinus) YANG DIINFEKSI Aeromonas hydrophila Angela Mariana Lusiastuti; Mohammad Faizal Ulkhaq; Widanarni Widanarni; Tri Heru Prihadi
Jurnal Riset Akuakultur Vol 11, No 2 (2016): (Juni 2016)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

The aim of this study was to evaluate the addition of a probiotic Bacillus on culture medium through growth rate and histopathological change in African Catfish who was infected by Aeromonas hydrophila. The study consisted of five treatments such as the addition of probiotic Bacillus ND2, Bacillus P4I2, Bacillus ND2 + Bacillus P4I2 (Kom), positive control (K+) and negative control (K-) (without probiotic addition). African Catfish (13.354±2.8 g) was maintained in 15 aquariums (40 L in volume) with 30 fishes each for 30 days. Probiotic bacteria was applied in water once a day, whereas pathogenic bacteria A. hydrophila (103 CFU/mL) were added once in earlier treatment (except for the negative control) as assumption that A. hydrophila will shape up quorum sensing in unfortunate condition. The result showed that the highest spesific growth rate in the treatment of Bacillus ND2 probiotics (1,708 ± 0.114%). Histopathology showed that damaged of liver dan kidney in probiotics treatment were lightner than positive control (K+). The addition of Bacillus P4I1 (104 CFU/mL) efective to increased the spesific growth rate of African Catfish and prevent the damage of liver and kidney of African Catfish was infected by Aeromoniasis.

Identification of Aeromonas Salmonicida and Edwardsiella Ictalury in Live Fish that Will Be Trafficked from Yogyakarta Special Region Anggi Rusita Rahmawati; Mohammad Faizal Ulkhaq; Dewi Susanti; Hapsari Kenconojati; Arif Habib Fasya
Journal of Marine and Coastal Science Vol. 10 No. 2 (2021): JUNE
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmcs.v10i2.27658

The freshwater aquaculture fishery sector in Indonesia has the potential to be developed through extensification and intensification. The problem in aquaculture is diseases that infects cultivated organisms, one of which is caused by bacterial infection. Bacterial infection can cause huge losses to cultivation activities, including death which results in economic losses. The purpose of this study is to identify the A. salmonicida and E. ictalury in live fish that will be exported from DI Yogyakarta. The method of examining bacteria used is conventional which consists of isolation, purification, presumptive test, biochemical test, and identification. The results showed that all live fish samples (koi, gouramy, goldfish, catfish, and eel) were not infected with A. salmonicida and E. ictalury. This shows that all live fish are in a healthy condition and suitable to be exported of DI Yogyakarta.

Pola Pertumbuhan Mikroalga Spirulina sp. Skala Laboratorium yang Dikultur Menggunakan Wadah yang Berbeda Nurita Wahyuni; Endang Dewi Masithah; Wiwie Soemarjati; Suciyono Suciyono; Mohammad Faizal Ulkhaq
Majalah Ilmiah Bahari Jogja Vol 16 No 2 (2018): Juli
Publisher : Sekolah Tinggi Maritim Yogyakarta

Penelitian ini bertujuan untuk membandingkan pola pertumbuhan Spirulina sp. yang dikultur pada wadah yang berbeda. Tahapan dalam penelitian ini yaitu persiapan dan sterilisasi wadah dan media, pembuatan media kultur Spirulina sp. dan vitamin, pemilihan inokulan Spirulina sp., dan kultur Spirulina sp. Hasil penelitian menunjukkan bahwa fase eksponensial pada wadah kaca terjadi pada hari ke-9 dengan total biomass sebesar 53,375 x 105 sel/ml, berbeda dengan wadah plastik yang terjadi pada hari ke-8 dengan total biomass sebesar 16,993 x 105 sel/ml.

PENGGUNAAN BUBUK ABATE UNTUK MENURUNKAN DERAJAT INFESTASI DAN MERUSAK ORGAN PARASIT Argulus YANG MENGINFESTASI IKAN MAS (Cyprinus carpio) Mohammad Faizal Ulkhaq
Journal of Aquaculture and Fish Health Vol. 8 No. 1 (2019): JAFH Vol. 8 No. 1 Februari 2019
Publisher : Department of Aquaculture

Kematian ikan akibat serangan parasit Argulus dikarenakan ikan mengalami emasiasi sehingga dapat menurunkan sistem kekebalan tubuh ikan, selain itu munculnya luka pada kulit ikan akan dimanfaatkan sebagai port of entry bagi agen infeksi sekunder seperti jamur, bakteri dan virus. Pengendalian penyakit argulosis dengan bahan kimia yang tidak tepat dosis dapat mencemari lingkungan, sehingga perlu dicari alternatif bahan kimia yang aman terhadap lingkungan, yaitu abate. Tujuan dari penelitian ini yaitu untuk mengetahui efektivitas perendaman abate untuk menurunkan derajat infestasi dan merusak organ parasit Argulus yang menginfestasi ikan mas (Cyprinus carpio). Perlakuan yang diberikan terdiri dari lima perlakuan dengan empat kali ulangan yaitu pemberian abate dengan konsentrasi 0 ppm (kontrol), 25 ppm, 50 ppm, 75 ppm, dan 100 ppm yang direndam selama 30 menit. Parameter yang diamati pada penelitian ini yaitu derajat infestasi Argulus yang menempel pada ikan mas, kerusakan organ Argulus dan nilai kualitas air pada awal dan akhir perendaman sebagai parameter pendukung. Data derajat infestasi Argulus pada ikan mas dianalisis secara statistik menggunakan ANOVA 5% yang dilanjutkan dengan uji DMRT 5% sedangkan kerusakan organ Argulus dianalisis secara deskriptif dan disajikan dalam bentuk gambar dan tabel serta dihubungkan dengan parameter pendukung. Hasil penelitian menujukkan bahwa perendaman abate tidak efektif untuk menurunkan derajat infestasi menginfestasi ikan mas (C. carpio), akan tetapi dapat menimbulkan kerusakan organ Argulus pada perlakuan 100 ppm. Kerusakan organ Argulus yang ditimbulkan yaitu rontoknya kaki ke-3 dan 4, abdomen yang menyatu serta produksi telur prematur. Perlu dilakukan penelitian lanjutan dengan meningkatkan dosis abate (lebih dari 100 ppm) serta waktu inkubasi yang lebih lama (lebih dari 30 menit). Selain itu perlu diamati perubahan fisiologis ikan selama dilakukan perendaman dalam abate.

Growth Response of Porphyridium sp. Culture on Glass and Plastic Container in Laboratory Scale Lutfiyah Al Adawiyah; Mohammad Faizal Ulkhaq; Hapsari Kenconojati
Journal of Aquaculture and Fish Health Vol. 9 No. 2 (2020): JAFH Vol. 9 No. 2 June 2020
Publisher : Department of Aquaculture

Utilization of Porphyridium sp. began to be developed as an anticancer, anti-inflammatory, antibacterial, antifungal, and production of biogas and biodiesel so that necessary to carry out culture activities for ensure continuity of Porphyridium sp. This study aims to determine the type of culture container that showed the best growth response of Porphyridium sp. with the highest biomass. The stages in this research were preparation of containers and media sterilization, making diatom fertilizers and agar media, Culture of Porphyridium sp. on agar media, Culture of Porphyridium sp. in the test tube, and Culture of Porphyridium sp. in glass and plastic containers. The results showed that the growth response of Porphyridium sp. that cultured in glass containers (18,9 ± 0,21 x 105 cells/mL) was higher than plastic (15.57 ± 0,03 x 105 cells/ml).

INCREASING PRODUCTIVITY OF FISH FARMERS THROUGH FISH HEALTH AND ENVIRONMENTAL MANAGEMENT TRAINING IN GENTENG DISTRICT, BANYUWANGI Hapsari Kenconojati; Mohammad Faizal Ulkhaq; Suciyono; Dewi Ambarwati; Sadida Anindya Bahtiar; Eko Wahyudi; Shovia Finny Anggraeni; Leni Mei Ristin
Jurnal Layanan Masyarakat Vol. 7 No. 1 (2023): JURNAL LAYANAN MASYARAKAT
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jlm.v7i1.2023.131-139

Disease is one of the main problems that have a major impact on aquaculture activities. Diseases in fish generally occur due to an imbalance of interactions between fish, pathogens, and the culture environment. Poor environmental quality can increase stress on fish and increase the pathogenicity of pathogens. In aquaculture activities, it is important to prevent disease in fish to avoid large economic losses. Based on the result of the initial survey, it was found that the problems faced by fish farmers in the Genteng area were the lack of knowledge in identifying the early syndrome of fish disease and the weakness in monitoring the quality of water for cultivation. This program aimed to provide the fish farmer with an introduction to disease kinds, training in environmental health management, and mentoring activities for aquaculture activities so that fish diseases can be prevented as early as possible. This programs were carried out on August 18, 2022, which was attended by 20 fish farmers in the Genteng area and continued with regular assistance for one month. Based on the result, there was an increase in understanding of the identification of parasites in fish, microscope utilization, and water quality test. In general, this programs received a positive response and enthusiasm from training participant, which was accompanied by an increase in participants’ knowledge about how to identify fish disease and water quality test.

Karakterisasi parasit pada komoditas perikanan di Balai Karantina Ikan, Pengendalian Mutu, dan Keamanan Hasil Perikanan Semarang, Jawa Tengah. Melinda Febrina; Mohammad Faizal Ulkhaq; Hayu Widyadi; Darmawan Setia Budi; Suciyono Suciyono
Depik Vol 9, No 3 (2020): December 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Parasite is one of the infectious disease pathogens in fish which can cause secondary infection by bacteria, fungi and viruses. Therefore, fish quarantine activities are needed to prevent disease transmission and are carried out by the fish quarantine center. This study aimed to characterize the types of parasites that infect fish commodities that will be trafficked at the Fish Quarantine Center, Quality Control and Safety of Fishery Products in Semarang, Central Java. Parasitic examination was carried out using the original microscopic method by scraping the mucus, gills and internal organs, then characterizing it morphologically. The results showed that the infected parasites in fish samples included ectoparasites (Trichodina, Gyrodactylus, Dactylogyrus, Chilodonella, and Argulus) and endoparasites (Anisakis).Keywords:ParasitesFishery CommodityBKIPMHP SemarangABSTRAKParasit merupakan salah satu patogen penyebab penyakit infeksius yang dapat memicu terjadinya infeksi sekunder oleh bakteri, jamur maupun virus. Oleh sebab itu, perlu dilakukan tindakan karantina ikan untuk mencegah terjadinya penyebaran penyakit yang dilakukan oleh balai karantina ikan. Penelitian ini bertujuan untuk mengkarakterisasi jenis parasit yang menginfeksi komoditas ikan yang akan dilalulintaskan di Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Semarang, Jawa Tengah. Metode penelitian yang digunakan yaitu pemeriksaan secara mikroskopis menggunakan metode natif/langsung pada lendir, insang dan organ dalam ikan, dilakukan karakterisasi secara morfologis. Hasil penelitian menunjukkan bahwa sampel ikan terinfeksi parasit dari golongan ektoparasit (Trichodina, Gyrodactylus, Dactylogyrus, Chilodonella, and Argulus) dan endoparasit (Anisakis).Kata kunci:ParasitKomoditas Perikanan BKIPMHP Semarang

Cyprinus carpio hematological changes after dietary Coffea canephora leaf extract for Aeromonas hydrophila infection Shandy Sulistyoningrum; Gunanti Mahasri; Mohammad Faizal Ulkhaq; Hapsari Kenconojati
Depik Vol 12, No 2 (2023): AUGUST 2023 (IN PROGRESS)
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.12.2.28682

Coffea canephora or robusta coffee was known as a medicinal plant that admitted various biological properties. However, its application as a medicinal plant in fish disease treatment has not been reported. This study aimed to investigate the effect of dietary robusta coffee leaf (RCL) extract on the survival rate and hematology of Cyprinus carpio infected by Aeromonas hydrophila. Two hundred fish were randomly divided into five treatment groups consisting of uninfected fish (UF), infected fish (IF), and a diet supplemented with different concentrations of RCL extract (30%, 40%, and 50%). The negative control group was injected by NaCl 0.9% and other treatment groups were injected by A. hydrophila. Feeding treatment was carried out for 7 days after clinical symptoms appeared. The results showed that RCL extract increases the survival rate and hematological parameters (number of erythrocytes, hemoglobin, and lymphocytes) significantly (P0.05). The highest survival rate (100%) was recorded in groups that accepted 50% of RCL extract. This current study indicates that RCL extract could be considered a potent medicinal plant for treating fish disease caused by A. hydrophila at the optimum dose of 50%.Keywords:AeromonasCoffea canephoraExtractHematologyCyprinus carpio

Karakterisasi parasit pada komoditas perikanan di Balai Karantina Ikan, Pengendalian Mutu, dan Keamanan Hasil Perikanan Semarang, Jawa Tengah. Melinda Febrina; Mohammad Faizal Ulkhaq; Hayu Widyadi; Darmawan Setia Budi; Suciyono Suciyono
Depik Vol 9, No 3 (2020): December 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.3.17693

Parasite is one of the infectious disease pathogens in fish which can cause secondary infection by bacteria, fungi and viruses. Therefore, fish quarantine activities are needed to prevent disease transmission and are carried out by the fish quarantine center. This study aimed to characterize the types of parasites that infect fish commodities that will be trafficked at the Fish Quarantine Center, Quality Control and Safety of Fishery Products in Semarang, Central Java. Parasitic examination was carried out using the original microscopic method by scraping the mucus, gills and internal organs, then characterizing it morphologically. The results showed that the infected parasites in fish samples included ectoparasites (Trichodina, Gyrodactylus, Dactylogyrus, Chilodonella, and Argulus) and endoparasites (Anisakis).Keywords:ParasitesFishery CommodityBKIPMHP SemarangABSTRAKParasit merupakan salah satu patogen penyebab penyakit infeksius yang dapat memicu terjadinya infeksi sekunder oleh bakteri, jamur maupun virus. Oleh sebab itu, perlu dilakukan tindakan karantina ikan untuk mencegah terjadinya penyebaran penyakit yang dilakukan oleh balai karantina ikan. Penelitian ini bertujuan untuk mengkarakterisasi jenis parasit yang menginfeksi komoditas ikan yang akan dilalulintaskan di Balai Karantina Ikan, Pengendalian Mutu dan Keamanan Hasil Perikanan Semarang, Jawa Tengah. Metode penelitian yang digunakan yaitu pemeriksaan secara mikroskopis menggunakan metode natif/langsung pada lendir, insang dan organ dalam ikan, dilakukan karakterisasi secara morfologis. Hasil penelitian menunjukkan bahwa sampel ikan terinfeksi parasit dari golongan ektoparasit (Trichodina, Gyrodactylus, Dactylogyrus, Chilodonella, and Argulus) dan endoparasit (Anisakis).Kata kunci:ParasitKomoditas Perikanan BKIPMHP Semarang

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