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Journal : Folia Medica Indonesiana

icaA/D Genes and Biofilm Formation of Methicillin-Resistant Staphylococcus aureus in Dr. Soetomo Hospital, Surabaya Putu Arya Suryanditha; Yoeke Dewi Rasita; Kartuti Debora; K Kuntaman
Folia Medica Indonesiana Vol. 54 No. 4 (2018): December
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.605 KB) | DOI: 10.20473/fmi.v54i4.10709

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a global health concern. One of the factors causing hospital infection is related to the ability of MRSA bacteria to form biofilms. Polysaccharide intercellular adhesin (PIA), encoded by ica gene, have an important role in S. aureus intracellular accumulation and aggregation. The aims of this study was to analyze the relationship between icaA, icaD genes and biofilm production in MRSA carrier and clinical isolate in Dr. Soetomo Hospital Surabaya. This study was an observational study using cross sectional approach. The sample was 47 MRSA isolates is as follow 28 isolates from carrier and 19 were clinical isolates. All of MRSA isolates carried mecA gene. PCR was performed to detect icaA and icaD genes. Biofilm formation was detected using microtiter plate assay (MTP). icaA gene was detected in all isolates whereas icaD gene in 96,4% carrier isolates and all (100%) of clinical isolates. Positive MTP results showed in all (100%) of carrier isolates and 57,9% of clinical isolates. Statistic result was significantly different in biofilm formation between carrier and clinical MRSA isolates. The proportion of positive biofilm formation in isolate with positive icaA/D genes was 82.6%. There was not any association between icaA and icaD gene with biofilm production.
Identification of Pathogenic Bacteria in Food Samples from Cafeterias of a University in Denpasar, Indonesia Anak Agung Ayu Lila Paramasatiari; Putu Indah Budiapsari; Putu Arya Suryanditha; Ni Wayan Widhidewi
Folia Medica Indonesiana Vol. 58 No. 4 (2022): December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (772.735 KB) | DOI: 10.20473/fmi.v58i4.34968

Abstract

Highlights: Eschericia colli was the most contaminant bacteria among food samples from Warmadewa Cafeterias Further suggestions to increase the hygiene in food processing must be offered.   Abstract : Foodborne diseases still remain a problem especially in public areas. This study aimed to identify pathogenic bacteria in foods sold at  the  cafeterias  of  Universitas Warmadewa,  Denpasar,  Indonesia.  The  presence  of  pathogenic bacteria  in  the  food  samples  was identified using the brain heart infusion (BHI) agar for the growth of Escherichia coli and Streptococcus aureus, selenite for Shigella and Salmonella, and alkaline peptone for Vibrio cholerae, then bacterial culture was conducted to identify the species. The results showed  that  Escherichia  coli  were  found  in  two  food  samples,  kangkong  and  jinggo  rice,  with  the  colony  counts  of  50-118. Escherichia coli was the most commonly found contaminant in food samples from the Warmadewa Cafeterias. Further suggestions must be offered to increase hygiene in the food processing by advising the stalls’ owners to serve good foods and conducting regular inspections to assess the food quality.