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Yulia Suciati
Universitas YARSI
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Journal : Universa Medicina

 1 
Typhonium flagelliforme decreases telomerase expression in HeLa cervical cancer cells Purwaningsih, Endang; Suciati, Yulia; Widayanti, Etty
Universa Medicina Vol 35, No 1 (2016)
Publisher : Faculty of Medicine, Trisakti University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2016.v35.3-9

Abstract

Background Cancer cells have a relatively high telomerase activity compared to normal cells, so that cancer cells have the ability for continued proliferation and uncontrolled mitosis. Telomerase is an enzyme responsible for the length of telomeres, DNA segments located at the ends of eukaryotic chromosomes. Natural materials such as rodent tuber (Typhonium flagelliforme) have anticancer potential. The purpose of the present study was to determine the effects of Typhonium flagelliforme extract on telomerase expression in HeLa cervical cancer and T47D breast cancer cells. Methods This experimental laboratory study was conducted on cultured HeLa and T47D cancer cell lines, with normal Vero cells as controls, and using RPMI and M199 culture media. The study comprised three groups, i.e. controls, and groups receiving Typhonium flagelliforme extract at doses of ½ IC50 and IC50. Telomerase expression was measured by immunohistochemistry (IHC). Analysis of variance and LSD multiple comparison test were used to analyze the data. Results Telomerase expression in cancer cells showed significantly higher values compared to normal Vero cells. Typhonium flagelliforme extract was capable of significantly decreasing telomerase expression in cancer cells receiving the extract. Conclusion Typhonium flagelliforme extract at different doses is capable of decreasing telomerase expression more effectively in cervical cancer cells than in breast cancer cells. This study shows that Typhonium flagelliforme may have anti-cancer activity, necessitating further investigations.
Cytotoxicity assay of Typhonium flagelliforme Lodd against breast and cervical cancer cells Endang Purwaningsih; Etty Widayanti; Yulia Suciati
Universa Medicina Vol. 33 No. 2 (2014)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2014.v33.75-82

Abstract

BACKGROUNDCancer is one of the causes of high mortality. Breast and cervical cancers are two of the most frequent cancers affecting women around the world, including Indonesia. Natural materials such as rodent tuber (Typhonium flagelliforme) have anticancer potentials. The rodent tuber extract contains ribosome inactivating proteins (RIPs) capable of cutting the DNA or RNA of cancer cells and blocking the growth of cancer cells. The purpose of this study was to evaluate the cytotoxic effects of Typhonium flagelliforme Lodd extract on HeLa cervical cancer and Michigan Cancer Foundation-7 (MCF-7) breast cancer cells.METHODSSubjects were cultured cell lines of HeLa cells in Rosswell Park Memorial Institute (RPMI) and of MCF-7 cells in Dulbecco’s Minimum Essential Medium (DMEM). Rodent tuber ethanolic extract was diluted in dimethyl sulfoxide (DMSO). The cytotoxicity assay used the 3-(4,5-dimethyl thiazol-2-yl,5-diphenyl) tetrazolium bromide (MTT) method. Absorbance was read in an ELISA reader at a wavelength of 595 nm.RESULTSRat tuber extract at all dilutions (500; 250; 125; 62.5; 31.25; 15.625;7.81; 3.9 ì g/ mL) showed cytotoxic effects against HeLa and MCF-7 cells. Higherconcentrations of the extract gave a higher proliferation inhibition effect.Calculated IC50 values of the extract by probit analysis were 30.19 ìg/mL against HeLa cells and 5.586 ì g/mL against MCF-7 cells.CONCLUSIONSEthanolic extract of Typhonium flagelliforme Lodd has cytotoxic effects against HeLa cells and MCF-7 cells. The cytotoxic effects against MCF-7 cells are greater than the cytotoxic effects against HeLa cells.
Ethanolic periwinkle leaf extract reduces telomerase expression in T47D cancer cells Endang Purwaningsih; Etty Widayanti; Yulia Suciati
Universa Medicina Vol. 34 No. 2 (2015)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2015.v34.79-86

Abstract

BACKGROUNDCancer cells have a relatively high telomerase activity and a lower p53 protein expression than normal cells, so that cancer cells have the ability to continue to proliferate and do not undergo apoptosis. One of the cancer treatments is chemotherapy using bioactive ingredients from synthesis or isolation of natural materials. One of the plants that have potential as anticancer agent is periwinkle (Catharanthus roseus L). The research objective was to evaluate the effect of ethanolic periwinkle leaf extract against p53 protein and telomerase expression in T47D cancer cells.  METHODSAn experimental study with controls was conducted involving T47D breast cancer cells. They were divided into 3 groups (control, ½ dose of IC50/26.849 µg/mL, and one dose of IC50/53.699 µg/mL) at a cell density of 1 x 104 cells/well. Expression of p53 and telomerase was measured by the immunohistochemistry method. Data were analyzed using one-way ANOVA followed by a multiple comparison test.RESULTSPeriwinkle leaf extract significantly increased p53 protein expression (p<0.05) at both treatment doses, ½ IC50 and IC50, compared to the control group and it highly significantly reduced telomerase expression (p<0.01), in comparison with the control group at both treatment doses. CONCLUSIONPeriwinkle leaf extract has potential as an anti-breast cancer agent by increasing p53 protein expression and inhibiting telomerase expression.
Ethanol extract of Abrus precatorius L. leaves diminishes inflammatory responses in nicotine-treated human gingival fibroblasts: an in vitro study Kesumaningtias, Raden Roro Widorini; Kusuma, Indra; Suciati, Yulia; Sari, Wening
Universa Medicina Vol. 43 No. 3 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.272-279

Abstract

BACKGROUNDNicotine induces oxidative stress in human gingival fibroblasts (HGF) and stimulates the production of cytokines that trigger inflammation. Abrus precatorius L. (AP) leaves contain antioxidants with anti-inflammatory properties that can prevent the formation of free radicals and reduce tissue damage due to inflammation. This study aimed to determine the effect of ethanolic extract of AP leaves (EAP) on interleukin (IL-6) levels and cyclooxygenase-2 (COX-2) gene expression in gingival fibroblasts exposed to nicotine. METHODSCells were randomized into six treatment groups and clustered into the non-treatment control group (NTC), solvent control (SC), nicotine control (NC), and groups treated with nicotine and EAP at doses of 9.375 µg/mL, 18.75 µg/mL, and 37.5 µg/mL, respectively, for 24 hours. IL-6 levels were examined using the Elisa method, while COX-2 gene expression was assessed using PCR. Data were analyzed using Oneway ANOVA and the Kruskal Wallis test. RESULTSIL-6 levels and COX-2 expression were considerably higher in the nicotine control group. Conversely, the cell groups treated with nicotine and EAP had substantially decreased levels of both inflammatory markers IL-6 and COX-2 (p=0.029) across all EAP dose levels compared to the nicotine control group. The highest reduction in response was observed at the dose of 9.375 ìg/mL EAP. CONCLUSIONThese results highlight the potential of Abrus precatorius L. in relieving nicotine-induced inflammation in smokers. By suppressing the production of inflammatory mediators IL-6 and COX-2 in HGF, EAP presents a promising avenue for further in vitro research.
Nicotine reduces cell viability and induces oxidative stress in human gingival fibroblasts Azmi, Sabrina; Hadi, Restu Syamsul; Kusuma, Indra; Suciati, Yulia; Sari, Wening
Universa Medicina Vol. 43 No. 1 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.20-30

Abstract

BackgroundNicotine, as the main component of cigarettes, is known to interfere with the proliferation of human gingival fibroblasts (HGFs) and can trigger oxidative stress. This study aimed to analyze the impact of nicotine on viability, expression of the antioxidant Nrf2, levels of the product of oxidative stress malondialdehyde (MDA), and the migration capacity of HGFs. MethodsAn experimental laboratory study used fibroblasts isolated from healthy human gingiva. The cells were grouped into the non-treatment control group (NTC), the solvent control (SC), and the treatment groups, exposed to nicotine at various concentrations for twenty-four hours. Cell viability was assesed using the cell counting kit-8 (CCK-8), Nrf2 expression was examined using ELISA, MDA level was measured using an MDA kit, and migration capacity was assessed using a scratch assay. Statistical analysis used one-way Anova or Kruskal-Wallis test. A p-value of <0.05 was expressed statistically significant. ResultsThe Cell viability was substantially reduced in the nicotine group compared to the untreated group, accompanied by changes in cell morphology. In contrast, Nrf2 expression increased significantly (p=0.010) in the 5 mM nicotine group compared with the control group. The MDA levels were not significantly distinct across groups (p=0.056). Cell migration was delayed significantly in the 5 mM nicotine group at 72 hours after scratching compared to the control group. ConclusionNicotine decreased HGFs viability and increased Nrf2 expression significantly in a dose-dependent manner. Nicotine at 5 mM concentration did not alter MDA levels but delayed cell migration.
Co-Authors Adifa Mazdalifah Anna P. Roswiem Aqillah Munawwarah Khairunnisa Aufa, Akhdan Azmi, Sabrina Bahri, Syukrini dedy suseno Dindana Caesarea Salsabila Diniwati Mukhtar Dita Safira Elsye Souvriyanti, Elsye Endang Purwaningsih Endang Purwaningsih Endang Purwaningsih Endang Purwaningsih Endy Muhammad Astiwara Erlina Burhan Erlina, Andi Etty Widayanti Etty Widayanti Etty Widayanti Etty Widayanti, Etty Fasli Jalal galuh Yendah Prasyad Gusnanto, Arief Indra Kusuma Kesumaningtias, Raden Roro Widorini Khairunnisa, Aqillah Munawwarah Khansadhia Hasmaradana Moiindie Linda Weni M Arsyad Mochamad Alif Ariesando Muchtiar, Mulyadi Muhammad Arsyad Muhammad Arsyad Muhammad Arsyad Muhammad Arsyad Nabila Wahyu Salsabilla Nunung Ainur Rahmah Pangestu, Hendri Punto Taufiknoor Putra, Andika Chandra Qomariyah Qomariyah Qomariyah Qomariyah Qomariyah Qomariyah Restu Syamsul Hadi Rifqatussaadah, Rifqatussaadah Rika Bur Rika Yuliwulandari, Rika Rizki, Anika Roswiem, Anna P. Saffa Hasanah Salsabila, Dindana Caesarea Salsabila, Nurul Sarah Kemalasari Suseno, Dedy TITIEK DJANNATUN Tjandra Yoga Aditama Triana Intan Sari Utomo, Ahmad Rusdan H Wan Nedra Wening Sari Wening Sari Widiyaningsih, Anggun Yenni Zulhamidah, Yenni Yenny Zulhamidah