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COMMUNITY EMPOWERMENT IN EFFORTS TO IMPROVE DENTAL AND ORAL HEALTH THROUGH TRAINING OF TRAINER ACTIVITIES IN “ANAK BANGSA ELEMENTARY SCHOOL” SURABAYA Widowati, Kharinna; Indarti, Caecilia; Sari, Rima Parwati; Prananingrum, Widyasri; Hollanda, Ghita Hadi; Emilda, Yulie; Parasihni, Kristanti; Karsini, Isidora; Elidasari, Monica; Agustia, Lita; Santoso, Budi; Lestari, IGAM Oka; Rizka, Yoifah; Hadinata, Yongki; Rochyani, Linda
Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Vol. 8 No. 1 (2024): MARET
Publisher : LPPM Univ. Ibn Khaldun Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/abdidos.v8i1.2221

Abstract

One way to develop health is community empowerment in the field of dental and oral health. Based on the 2018 Basic Health Research, it was reported that the prevalence of dental and oral problems in Indonesia is still very high. As many as 93% of school-aged children experience dental and oral problems. This is due to a lack of knowledge and lack of awareness of the Indonesian population regarding dental hygiene. In a series of activities for the 2023 National Dental Health Month, dental and oral health education programs are carried out in schools/School Health Programs. Method: The method is carried out using a cadre training program / Training of Trainers through zoom meetings for teachers and parents of students by providing dental and oral health material. Next, ask them to provide assistance with morning and evening toothbrushing activities for 21 days, teaching children about the benefits of good oral hygiene and showing them how to brush their teeth twice a day. It is preceded by a pretest and at the end of the delivery of the material a post test is given. The results of the evaluation of understanding of the material presented show that there is a significant difference in the pre-test and post-test of prospective cadres, which means that the cadres understand the material well. The new habit of brushing teeth has been quite successful from the uploaded photo documentation and students have recorded well for 21 days. This shows a change in behavior in maintaining dental and oral health.
Cytotoxicity and mechanical properties of biphasic calcium phosphate scaffold from Tegilarca granosa due to its composition Prananingrum, Widyasri; Caroline, Grace; Rizal, Mohammad Basroni; Prabowo, Puguh Bayu; Pratama, Afif Fahwi; Resaldi, Muhammad Firdan; Annisa, Nindya Yuanita; Fadhilah, Yusti; Sari, Rima Parwati
Odonto : Dental Journal Vol 10, No 2 (2023): December 2023
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.10.2.247-256

Abstract

Background: Biphasic calcium phosphate (BCP) is graft material contained HA and TCP. Tegilarca granosa shell is a natural source that may converted into BCP. This study aims to determine the composition and cytotoxicity of BCP synthesized from Tegilarca granosa shell used various hydrothermal hours and to evaluate the mechanical properties of BCP scaffold. Methods: Tegilarca granosa shell was converted into BCP using hydrothermal method at 200˚C for 6h (Group 1); 9h (Group 2); and 12h (Group 3). The composition was determined by XRD and the cell viability were evaluated using MTT Assays. Each group was added with 20% gelatin ratio 50:50 (w/v) and freeze-dried to form scaffold. Scaffolds (Ø6mm x 4mm) were prepared for diametral tensile strength (DTS) test (n=6) and scaffolds (Ø7mm x 11mm) were used for compressive strength (CS) test (n=6). All data were analyzed using Kruskall-Wallis followed by Mann-Whitney test. Results: The composition of BCP (HA/ TCP) at Group 1, Group 2, and Group 3 were 81.80%/14,10%; 87%/6%; and 72%/21%. The cell viabilities were good for all groups. The DTS and CS test showed there was a significant difference between Group 1 and Group 3 scaffold, meanwhile there was no significant differences between Group 2 and Group 3 scaffold. Group 3 scaffold showed the highest DTS and CS, 6.921 MPa and 1,233 MPa. Conclusion: The BCP composition were depent on hydrothermal hours. Although all scaffold groups were non-toxic, but BCP scaffold synthesized from Tegilarca granosa shell using hydrothermal for 12 hours showed the highest mechanical properties.
The number of osteoblasts and osteoclasts in bone remodeling of bone defects caused by peri-implantitis using Anadara granosa bone graft: an experimental study Ariestania, Vivin; Hendrijantini, Nike; Prahasanti, Chiquita; Kurniawan, Hansen; Ashrin, Meinar Nur; Nanik, Chaterina Diyah; Apsari, Anindita; Megantara, Rizko Wira Artha; Sari, Rima Parwati; Hermanto, eddy; Fauzia, Bunga; Matsuyama, Miwa
Padjadjaran Journal of Dentistry Vol 37, No 3 (2025): November 2025
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol37no3.64295

Abstract

Introduction: Peri-implantitis treatment aims to replace the damaged bone with new and healthy tissue during bone remodeling process. Bone grafts are materials used to stimulate the formation of new bone. Bone graft material derived from Anadara granosa (AG) can be synthesized into hydroxyapatite–tricalcium phosphate (HA-TCP) at a 70:30 ratio, which supports bone regeneration, as indicated by an increase in osteoblast numbers and decrease in osteoclast numbers. This study aims to analyze the effectiveness of Anadara granosa bone graft in the number of osteoblasts and osteoclasts during bone remodeling process. Methods: This study employed an experimental design with a post-test only control group. A total of 84 mice were divided into 12 groups (7 mice per group): negative control (K−), positive control (K+), and treatment group (P), each observed on days 14 and 28. Histological analysis was performed to count osteoblasts and osteoclasts. Data were analyzed using one-way ANOVA. Results: The number of osteoclasts was significantly reduced in the treatment groups (P14: 7.00 ± 1.528; P28: 6.57 ± 1.512) compared to the positive controls (K+14: 13.86 ± 2.410; K+28: 14.29 ± 1.496). On the contrary, the number of osteoblasts increased in the treatment groups (P14: 7.14 ± 1.676; P28: 8.57 ± 1.272) compared to the positive controls (K+14: 2.57 ± 1.512; K+28: 3.86 ± 1.574). Statistical analysis indicated that osteoblasts showed significant differences after AG treatment (p<0.05), and the ANOVA test showed significant differences in osteoclast number after AG treatment (p<0.05).      Conclusion: The number of osteoblasts increases while the number of osteoclast reduces in bone remodelling of bone defect caused by peri-implantitis using AG bone graft.
THE EFFECTIVITY OF SCAFFOLD FROM ANADARA GRANOSA SHELL-STICHOPUS HERMANNI ON BLOOD VESSEL COUNTS AFTER TOOTH EXTRACTION Sari, Rima Parwati; Kusumawardani, Chaterina Dyah Nanik; Rahayu, Retno Pudji; Soedjarwo, Sri Agoes; Damaiyanti, Dian Widya
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.576 KB) | DOI: 10.30659/odj.8.2.11-17

Abstract

ABSTRACTBackground: Alveolar bone resorption can occur after tooth extraction. One source of bone scaffold material is hydroxyapatite and tricalcium phosphate (HA-TCP). The shell of Anadara granosa can be synthesized to be HA-TCP. Hyaluronic acid, which is widely contained in Stichopus hermanni, can stimulate endothelial progenitor cells for the healing process. Purpose: This research aims to prove the Effectivity of scaffold from Anadara granosa shell-Stichopus hermanni on blood vessel counts after tooth extraction. Methods: The sample in this study was male Wistar rats divided into four study groups randomly. Each group consists of 6 rats. Extraction was carried out on the mandibular first incisor, then was given gelatin as a placebo in the control group, treatment groups given scaffold from Anadara granosa shells (AG), and scaffold combination from Anadara granosa shells-Stichopus hermanni with concentrations of 0.4%-0.8%. (AGSH1-AGSH2). Development of HA-TCP synthesized from Anadara granosa combined with Stichopus hermanni extract for biomedical scaffolds using the freeze-dried method. Observations were made three days after tooth extraction. Data analysis has used one-way ANOVA followed by Tukey HSD (p<0.05). Results: The results of the ANOVA test followed by the HSD-Tukey test showed a significant difference between C (7.33±1.51) and AGSH1 (11.50±1.38), AGSH2 (21.17±1.94), AG (9.33±1.63) and AGSH1 with AGSH2, but between AG and, AGSH1 no significant differences.Conclusions: Combination scaffold from Anadara granosa shell and Stichopus hermanni 0.8% effectively increased blood vessel counts after tooth extraction.
CHARACTERIZATION AND POTENCY OF STICHOPUS HERMANNI ETHANOL EXTRACT ON ORAL WOUND HEALING Sari, Rima Parwati; Sugiarto Budijono, Stevanus Candra
Odonto : Dental Journal Vol 7, No 1 (2020): July 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (875.218 KB) | DOI: 10.30659/odj.7.1.9-17

Abstract

Background: Stichopus hermanni contains protein, glycosaminoglycans and EPA-DHA, which accelerate oral wound healing. Ethanol is solvent with universal nature and frequently used in the extraction. This study aims to know the characterization and potency of Stichopus hermanni in oral wound healing. Methods: Stichopus hermanni was dried by freeze-dry method and extracted using ethanol solvent. Characterization was examined the levels of protein, glycosaminoglycans and EPA-DHA. In vivo, male wistar rats made traumatic ulcer at the lower labial mucosa by using thermal burn. Thirty-two rats are divided into four groups (n=8), negative control group (K), positive control group (AH) was given hyaluronic acid gel 0.2%, and treatment groups were given Stichopus hermanni 60%-80% (SH60-SH80) gel. The ulcer formed was given gel for four days. The rat’s labial mucosa was measured. The difference between the first and fourth day is tabulated. Data were analyzed by Anova and Tukey-HSD tests. Results: The result of Spectrophotometry show the contents of protein (19.39), glycosaminoglycans (2.79–3.98). The result of in vivo showed a significant difference in ANOVA test (K=1.07; AH=1.27; SH60=1.89; SH80=1.66). In the Tukey-HSD test, the SH60 group shows a significant difference between K and AH groups. Conclusion: Stichopus hermanni ethanol extract gel contain protein and Glycosaminoglycans and potency for oral wound healing.
Characteristics of biphasic calcium phosphate scaffold formed by Anadara granosa shell synthesis Sari, Rima Parwati; Sari, Maharani Dewinta; Ramanindia, Andiani; Fitri, Karimatul; Susanto, Michelle Rosalind; Pratiwi, Safira Agnes; Yusuf, Diajeng Ayu Permatasari; Prananingrum, Widyasri; Ariestania, Vivin; Widaningsih; Apsari, Anindita; Widyastuti; Brahmanta, Arya; Hermanto, Eddy
Dental Journal (Majalah Kedokteran Gigi) Vol. 59 No. 1 (2026)
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v59.i1.p78-86

Abstract

Background: Bone augmentation requires biomaterials that can trigger bone regeneration, especially when treating alveolar bone defects. Biphasic calcium phosphate (BCP) can induce cell proliferation and differentiation. Anadara granosa shell can be synthesized into BCP and made into a scaffold with the addition of gelatin. Purpose: To determine the optimal BCP ratio from A. granosa shell synthesis and to establish the material characteristics. Methods: Anadara granosa shells were synthesized using the hydrothermal method at 200°C for 12 and 18 hours, followed by particle size analysis. Next, the resulting powder was made into scaffolds by adding gelatin through the freeze-drying method, and a series of observations were performed regarding its morphology, pore size, porosity, compressive strength (CS), and cell viability. The data were analyzed using an independent t-test, with p < 0.05. Results: Synthesis of A. granosa shells for 12 hours produced a BCP ratio of 69:20 with 11% calcite content and a particle size of 3,343.00 ± 445.25 nm, whereas synthesis for 18 hours produced a ratio of 22:78 with a particle size of 919.96 ± 375.77 nm. In scaffold manufacture, the BCP18 pore size (26.56–145.45) was smaller than that of BCP12 (19.72–309.09), the porosity level was lower (61.49% vs. 81.98%, respectively), and the CS was higher (4.66 ± 1.12 vs. 2.89 ± 0.799, respectively). Nevertheless, both had relatively good cell viability (≥50%). Conclusion: Biphasic calcium phosphate obtained from A. granosa shell synthesis exhibits varying ratios; nevertheless, both variants possess favorable attributes for consideration as potential bone substitute materials, as they meet the necessary biocompatibility requirements.