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All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Akuakultur Rawa Indonesia Jurnal Lahan Suboptimal Omni-Akuatika JURNAL GALUNG TROPIKA Jurnal Riset Akuakultur Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Seminar Nasional Lahan Suboptimal Menara Perkebunan Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Journal of Aquaculture and Fish Health Jurnal Abdimas Mahakam Jurnal Mangifera Edu Jurnal Akuakultura Universitas Teuku Umar Jurnal Abdi Insani SRIWIJAYA JOURNAL OF ENVIRONMENT Makara Journal of Science Journal of Fish Health Jurnal Ruaya : Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Depik Jurnal Ilmu-Ilmu Perairan, Pesisir, dan Perikanan Journal of Fisheries & Marine JARI : Jurnal Akuakultur Rawa Indonesia
Marini Wijayanti
Universitas Sriwijaya
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Mathematics Public Health Social Sciences Veterinary Other

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Journal : Menara Perkebunan

 1 
Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum . TRI-PANJI; . SUHARYANTO; Marini WIJAYANTI
E-Journal Menara Perkebunan Vol 77, No 1: Juni 2009
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.392 KB) | DOI: 10.22302/iribb.jur.mp.v77i1.113

Abstract

AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas
Produksi, isolasi dan karakterisasi superoksida dismutase dari Spirulina platensis yang dibiakkan dalam serum lateks Production, isolation, and characterization of superoxyde dismutase from Spirulina platensis cultured on latex serum . TRI-PANJI; . SUHARYANTO; Marini WIJAYANTI
Menara Perkebunan Vol. 77 No. 1: 77 (1), 2009
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v77i1.113

Abstract

AbstractSpirulina platensis is a blue-green microalgawhich is frequently used for food and feedsupplements and cosmetic active agent. Thismicroalga also produces a strong antioxidantnamely superoxide dismutase (SOD) used ascosmetic active agent for anti aging and anti freeradicals. SOD was isolated from S. platensis cellbiomass from local isolate grown on latex serumon semipilot (3.5 m 3 ) and pilot scale (40 m 3 )then dried with spray drying or sun drying andcharacterized. SOD was purified with sequentialtwo-stage sedimentation using ammoniumsulphate and fractionated in chromatographiccolumn containing Sephadex G 200. Thefractions were analysed to determine the activity,cofactor metal and amino acid composition of theantioxidant. The results showed thatsedimentation of SOD extract with 80%ammonium sulphate produced SOD with higheractivity compared to that of SOD fromcommercial S. platensis biomass. This SOD wassuccessfully isolated and purified. MetaloenzymeSOD was composed of subunits with molecularweight of 77.78; 71.74; and 19.2 kDa, whichcontained nine types of amino acids with tyrosineand lysine as the major amino acid components.Zn was the most predominant metal on SOD, thenfollowed by Fe and Mn. The main subunitcofactors consisted of Zn 72%, Fe 25%, Mn 2%,and Cu 1%, which were different from thesmall subunit that contained of Zn 55%, Mn 31%,Fe 14%, and Cu 4%. The stability of SOD wasachieved on pH 7.5 and temperature below 25 o C.AbstrakSpirulina platensis adalah mikroalga hijaubiru yang banyak digunakan sebagai suplemenpangan, pakan, dan bahan aktif kosmetika.Mikroalga ini juga menghasilkan antioksidankuat yaitu superoksida dismutase (SOD), yangmerupakan bahan aktif kosmetika anti penuaandini dan pencegah efek radikal bebas. SODdiisolasi dari biomassa sel S. platensis isolat lokalyang dibiakkan dalam serum lateks skalasemipilot (3,5 m 3 ) dan pilot (40 m 3 ) sertadikeringkan dengan cara pengeringan kabut(spray drying) atau penjemuran untuk kemudiandikarakterisasi. SOD dimurnikan dengan peng-endapan bertingkat menggunakan ammoniumsulfat dan dipisahkan dengan kolom kromatografiberisi Sephadex G 200. Hasil pemisahankemudian dianalisis untuk menentukan aktivitas,logam kofaktor serta komposisi asam amino antioksidan tersebut. Hasil penelitian menunjukkanbahwa pengendapan ekstrak SOD denganSOD lebih tinggi dari SOD asal biomassaS. platensis komersial. SOD tersebut telahberhasil diisolasi dan dimurnikan. MetaloenzimSOD tersusun atas subunit dengan BM 77,78;71,74; dan 19,2 kDa, yang mengandungsembilan jenis asam amino dengan tirosin danlisin sebagai komponen asam amino utama.Logam yang dominan pada SOD adalah Zn,disusul kemudian Fe dan Mn. Kofaktor sub unitbesar terdiri dari Zn 72%, Fe 25%, Mn 2%, danCu 1%, berbeda dengan sub unit kecil yangmengandung Zn 55%, Mn 31%, Fe 14%, dan Cu4%. Stabilitas SOD S. platensis dicapai pada pH7,5 dan suhu di bawah 25 o Cammonium sulfat 80% menghasilkan aktivitas
Co-Authors Ade Bayu Saputra Ade Dwi Sasanti Ade Dwi Sasanti Ade Dwi Sasanti Afriansyah, Azmi Agustina Aldila Din Pangawikan Andini, Andini Anja Meryandini Anjastari, Devi Annisa Siregar Arafsanajani Arif Aris Tri Wahyudi Aritonang, Lilis Pritamasari Chandra Irsan Choirul Amar Simbolon Citra, Nyayu Dade - Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Dade Jubaedah Danang Yonarta Danang Yonarta Dedi . Apriadi Devi Anjastari Dwi Rizki Septiana Eka Febri Fitriana Engelhaupt, Martin Erwin Nofyan Feni Haryani Ferdinand Hukama Taqwa Ferdinand Hukama Taqwa Fitriana, Eka Febri Genti, Karta Sari Gracia Eirene Girsang Hary Widjajanti Hendro Widyantoro Herdia Agustina Hijral Hamdani Husnul Khotimah Ida Handayani Ismalia, Nova Junedi Tinambunan Kezia Agustine Riyadhi Kukuh Jaya Langgeng Priyanto, Langgeng M Aiman Ziyad M Syaifudin Madyasta Anggana Rarassari Madyasta Anggana Rarassari Madyasta Anggana Rarassari Madyasta Anggana Rarassari Maemonah, Maemonah Marsi Marsi - - Marsi Marsi Marsi Marsi Marsi Marsi Marsi Marsi Marsi Marsi Maswala, Depi Maulidya, Ainayyah Mirna Fitrani Mirna Fitrani Mirna Fitrani Mochamad Syaifudin, Mochamad Mohamad Amin Muhammad Fadhil Akbar Mulyani, Yenni Sri MUNTI YUHANA Muslim Muslim Muslim Muslim Nabila Saraswati Novi Wulandari Mustika NUNI GOFAR Nurwijayanti Nyayu Nurul Husna Olivia Riana Sari Rarassari, Madyasta Anggana Retno Cahya Mukti Ria octaviani Riswandi, Agung Saputra, Ade Bayu Sefti Heza Dwinanti Sefti Heza Dwinanti Sefti Heza Dwinanti Sefti Heza Dwinanti Septika Putri Anggraini Septimesy, Annisa Septiyani, Reni Siti Yuliani Sofiatul Rahmani Soikar Hariadi Suharyanto Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur Tanbiyaskur, Tanbiyaskur Tri Panji Valencia Aff Neka Wati, Rizka Yonarta, Danang Yonarta, Danang Yudha Galih Wibawa YULISMAN Yulisman Yulisman Yulisman Yulisman