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Identifikasi dan Deteksi Aktivitas Daya Hambat Bakteri Actinomycetes yang diisolasi dari Tanah Gambut di Desa Tajok Kayong Kalimantan Barat Rikhsan Kurniatuhadi, Sri Lestari, Mukarlina,
Jurnal Protobiont Vol 8, No 1 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i1.30843

Abstract

Actinomycetes bacteria can grow well in soil media with acidic pH conditions such as peat soil. This study aims to determine the genus of Actinomycetes bacteria from peat soil. Sampling was done in Tajok Kayong Village, Ketapang Regency. Isolation was carried out using Pour Plate Method using specific media, namely SCA (Strach Casein Agar) media, while the activity detection of Actinomycetes bacteria was carried out on Eschericia coli pathogenic bacteria and Staphylococcus aureus using the streak-cross Method. Identification and  characterization done it macroscopic, microscopic and biochemical. The results obtained 7 isolates of Actinomycetes bacteria from peat soil which is the genus Streptomyces.
Daya Hambat Ekstrak Etanol Batang Lakum (Cayratia trifolia (L.) Domin) Terhadap Pertumbuhan Isolat Phytophthora sp. Im5 secara In Vitro Rikhsan Kurniatuhadi, Fitrie Dwi Lestari Elvi Rusmiyanto PW
Jurnal Protobiont Vol 7, No 3 (2018)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v7i3.29064

Abstract

Stem rot disease is caused by one of Phytophthora pathogenic fungi that attacks several types of cultivated plants. The alternative control against Phytophthora attack can be done with the use of natural fungicides. One of the herbs that can be untilized is the ethanol extract of lakum stem (Cayratia trifolia (L.) Domin). The aim of this research is to know the effect of ethanol extract of lakum stem and its secondary metabolite in inhibiting the growth of isolate Phytophthora sp. Im5. The research was conducted for three months starting from October to December 2017. This research used solid dilution method and completely randomized design (CRD) wth 8 levels of treatments, i.e. positive control, negative control, ethanol extract concentration of 5, 10, 15, 20, 25 and 30 mg/ml. The test for secondary metabolite compounds was done by phytochemical screening method. The result showed that the concentration of 30 mg/ml has the biggest antifugal activity with the inhibition percentage of 34.98%. The activity of lakum stem ethanol extract against isolate Phytophthora sp. Im5 was included in moderate catagory. The results of phytochemical screening showed that the ethanol extract of lakum stem contained alkaloid, flavonoid and tannin compounds.
Deteksi Keberadaan Bakteri Staphylococcus di Udara Dalam Ruangan Pasar Tradisional Kota Pontianak Rikhsan Kurniatuhadi, M. Arief Faturrahman, Rahmawati,
Jurnal Protobiont Vol 8, No 2 (2019)
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/protobiont.v8i2.32479

Abstract

Bacteria belongs to the genus of Staphylococcus is pathogenic bacteria and its existence is an indicator of contaminated environment particularly in the air. The aim of this study is to detect the existence of bacteria belongs to Staphylococcus in the traditional market space of Pontianak city. The study was conducted in January - March 2018. Sampling was administered in 4 traditional markets of Pontianak City using volumetric air sampling along with a vacuum air pump YYP 550-12A1 with an air flow rate of 26 l / min for 6 minutes using Mannitol Salt Agar (MSA). The results showed that air bacteria member of the genus Staphylococcus found in all traditional markets in the city of Pontianak. The lowest number of bacteria belongs to the lowest Staphylococcus genus in the Flamboyan Market was 15 CFU/m3 and the highest was in Pasar Mawar 89 CFU/m3. The data indicates the air in the traditional market of Pontianak City has not fully fulfill the air health standards according to Sedyaningsih (2011), which is free of pathogenic bacteria 0 CFU/m3.
Peningkatan Kepedulian Masyarakat Kecamatan Pontianak Timur Terhadap Kesehatan Melalui Media Leaflet Rousdy, Diah Wulandari; Linda, Riza; Kustiati, Kustiati; Yanti, Ari Hepi; Rahmawati, Rahmawati; Riyandi, Riyandi; Junardi, Junardi; Ifadatin, Siti; Rusmiyanto, Elvi; Setyawati, Tri Rima; Kurniatuhadi, Rikhsan; Rafdinal, Rafdinal; Turnip, Masnur; Zakiah, Zulfa; Mukarlina, Mukarlina; Lovadi, Irwan; Saputra, Firman
Jurnal Pengabdi Vol 4, No 1 (2021): April 2021
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jplp2km.v4i1.44379

Abstract

Kesehatan merupakan faktor kebutuhan utama manusia untuk dapat melangsungkan aktivitas hidup dengan sempurna. Kesehatan masyarakat terganggu bila terdapat kuman yang menginfeksi tubuh. Corona  atau Severe  Acute  Respiratory  Syndrome Coronavirus  2 (SARS- CoV-2) adalah virus yang menyerang  sistem pernapasan. Penyakit ini dapat menyebabkan infeksi paru-paru yang berat, hingga kematian. Data bulan April 2020, Kecamatan Pontianak Timur dilaporkan mempunyai angka kasus positif Covid-19 paling tinggi diantara kecamatan lain di Kota Pontianak.  Kelurahan di Kecamatan Pontianak Timur yang dilaporkan mempunyai angka kasus Covid-19 antara lain Kelurahan Tanjung Hulu, Kelurahan Parit Mayor, Kelurahan Banjar Serasan, Kelurahan Tambelan Sampit, Kelurahan Dalam Bugis dan Kelurahan Tanjung Hilir. Kurangnya kesadaran masyarakat tentang kesehatan dan cara mengatasi permasalahan kesehatan sangat perlu dilakukan  suatu bentuk sosialisasi dengan cara memberikan edukasi tentang permasalahan kesehatan dan cara mengatasinya. Cara yang sangat memungkinkan pada masa pandemi ini adalah dengan tidak langsung bertatap muka namun dapat dilakukan secara tidak langsung yaitu melalui leaflet dan contoh produk.  Tujuan dari kegiatan ini adalah memberikan edukasi kesehatan dalam mencegah Covid-19 kepada masyarakat. Metode yang dilakukan dalam kegiatan ini adalah memberikan edukasi dan penyuluhan melalui leaflet yaitu cara pembuatan hand sanitizer dan masker serta contoh  produk hand sanitizer dan masker yang diberikan kepada masyarakat. Sebelum pelaksanaan kegiatan, sebagian besar masyarakat (90%) di Kecamatan Pontianak Timur belum menerapkan protokol pencegahan Covid-19 yang benar. Setelah pelaksanaan kegiatan diperoleh hasil berupa peningkatan kesadaran dan pengetahuan masyarakat (100%, n=100 orang) mengenai upaya pencegahan Covid-19 serta cara pembuatan masker dan hand sanitizer.
Kualitas Gaharu Aquilaria sp. dengan Pemberian Bioinokulan Fermentasi Batang Pisang yang Terkena Penyakit Layu Fusarium selno, Silvester; Zakiah, Zulfa; Kurniatuhadi, Rikhsan
JURNAL BIOS LOGOS Vol 11, No 2 (2021): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.11.2.2021.32551

Abstract

(Article History: Received February 1, 2021; Revised March 8, 2021; Accepted April 7, 2021) ABSTRAK Upaya produksi gaharu budidaya umumnya menggunakan inokulan dari jenis Fusarium sp., namun mahalnya inokulan biakan murni menjadi faktor pembatas untuk produksi gaharu budidaya. Bioinokulan dapat menjadi solusi bagi petani gaharu karena dibuat dari bahan yang mengandung mikroorganisme patogen dan tidak membutuhkan proses pembiakan murni. Penelitian ini bertujuan untuk mengidentifikasi jenis-jenis mikroorganisme dan konsentrasi bioinokulan yang tepat digunakan untuk menghasilkan kualitas gaharu yang baik serta mengetahui kualitas gaharu yang dihasilkan dengan menggunakan bioinokulan dari fermentasi batang pisang yang terkena penyakit layu fusarium. Metode penelitian lapangan dengan melakukan inokulasi bioinokulan dari fermentasi batang pisang yang terkena penyakit layu fusarium pada pohon Aquilaria sp. Setiap konsentrasi diinokulasikan pada pohon yang berbeda. Pada ketinggian 30 cm dari permukaan tanah, pohon dibuat lubang sebanyak 5 buah secara vertikal dengan menggunakan bor dengan jarak antar lubang 50 cm. Inokulan diinokulasikan melalui lubang yang telah dibuat dengan metode infus. Hasil penelitian diperoleh tiga jenis mikroorganisme yang terdapat pada bioinokulan yaitu kelompok genus Aspergillus, Fusarium dan Saccharomyces. Perlakuan dengan konsentrasi bioinokulan 140 ml memberikan hasil yang terbaik terhadap bobot kayu gaharu, warna, aroma dan kadar resin gaharu yang dihasilkan. Berdasarkan penelitian ini dapat disimpulkan bahwa kualitas gaharu yang dihasilkan setelah 4 bulan inokulasi menghasilkan mutu gaharu kelas kemedangan. Kata Kunci: Aquilaria sp., Bioinokulan, Fusarium sp., Gaharu, Inokulasi ABSTRACT Efforts to produce cultivated agarwood generally use inoculants of the type Fusarium sp. however the high cost of pure culture inoculants is a limiting factor for cultivated agarwood production. Bioinoculants can be a solution for agarwood farmers because they are made from materials containing pathogenic microorganisms and do not require a pure breeding process. The research aims to determine the kind of microorganisms and the proper concentration of bioinoculants to produce good quality agarwood and determine the quality of aloes produced by using bioinoculants from fermented banana stem affected by fusarium wilt disease. The method of field research was by conducting inoculation of bioinoculants from the fermentation of banana stems affected by fusarium wilt on Aquilaria sp. Each concentration was inoculated on a different tree. At a height of 30 cm from the ground, 5 holes are made vertically using a drill with a distance of 50 cm between the holes. The inoculant is inoculated through the hole that has been made by the infusion method. The results showed that there were three types of microorganisms found in bioinoculants, namely the Aspergillus, Fusarium and Saccharomyces genus groups. Treatment with a concentration of 140 ml of bioinoculant gave the best results on the weight of agarwood, color, aroma and content of the resulting agarwood resin. Based on this research, it can be concluded that the quality of aloes produced after 4 months of inoculation resulted in the quality of agarwood in the kemedangan class.Keywords: Agarwood, Aquilaria sp., Bioinoculants, Fusarium sp., Inoculation
Uji Antagonis Pseudomonas flourescens spp. Terhadap Isolat Bakteri Xanthomonas (SL3) dari Daun Padi Bergejala Hawar di Kabupaten Kubu Raya Larasaty, Suprih; Mukarlina, Mukarlina; Kurniatuhadi, Rikhsan
JURNAL BIOS LOGOS Vol 11, No 1 (2021): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.11.1.2021.30998

Abstract

(Article History: Received 21 October 2020; Revised 5 November 2020; Accepted 11 November 2020) ABSTRAKPenyakit hawar pada tanaman padi disebabkan oleh bakteri anggota genus Xanthomonas. Pengendalian penyakit tanaman dapat menggunakan bakteri sebagai Agen Pengendalian Hayati (APH) yang bersifat antagonis terhadap patogen. Pseudomonas flourescens merupakan APH yang memiliki kemampuan penghambatan terhadap bakteri patogen Xanthomonas. P. flourescens spp yang diisolasi dari rhizosfer berbeda memiliki kemampuan penghambatan yang berbeda. Penelitian ini bertujuan untuk menguji kemampuan daya hambat bakteri P. flourescens spp. yang berasal dari rhizosfer yang berbeda terhadap bakteri Xanthomonas yang diisolasi dari daun padi bergejala hawar di Kabupaten Kubu Raya. Penelitian ini dilakukan pada bulan Mei 2019 bertempat di Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak. Pengambilan sampel dilakukan di sentra produksi padi Desa Limbung Kabupaten Kubu Raya. Uji antagonis dilakukan di Laboratorium Mikrobiologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Tanjungpura Pontianak menggunakan metode Kirby-bauer. Hasil uji antagonis menunjukkan kedua bakteri P. flourescens spp. mampu menghambat isolat bakteri genus Xanthomonas. Jenis P. flourescens spp (Sgu5) memiliki kemampuan penghambatan terbaik hingga mencapai 8,43 mm.Kata kunci: Agen pengendalian hayati; Penyakit Hawar; Pseudomonas flourescens; Xanthomonas (SL3) ABSTRACTBlight disease in rice plants is caused by bacteria belonging to the genus Xanthomonas. Plant disease control can use the Biological Control Agent (APH) which is antagonistic to pathogens.Pseudomonas flourescens is an APH that has the ability to inhibit the pathogenic bacteria Xanthomonas. Pseudomonas flourescens spp. isolated from different rhizosphere had different inhibitory abilities. This study aims to determine the inhibitory ability of the bacteria Pseudomonas flourescens spp. against bacteria isolated from blight with symptomatic rice in Kubu Raya Regency. The research was conducted in my 2019 at the Biology Department, Faculty of Mathematics and Natural Sciences, Tanjungpura University Pontianak. Sampling was carried out at the rice production center in Limbung Village, Kubu Raya Regency.The antagonist test was carried out at the Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, Tanjungpura University, Pontianak using the Kirby-bauer method. The antagonist test result showed that both Pseudomonas flourescens spp. able to inhibit bacterial isolates of the genus Xanthomonas. Pseudomonas flourescens spp. (Sgu5) has the best inhibition ability up to 8.43 mm.Keywords: Biological control agent; Blight disease; Pseudomonas flourescens; Xanthomonas (SL3)
Karakterisasi Kapang dari Saluran Pencernaan Cacing Nipah (Namalycastis rhodochorde) Asal Desa Sungai Kakap, Kabupaten Kubu Raya, Kalimantan Barat Yanti, Ari Hepi; Setyawati, Tri Rima; Kurniatuhadi, Rikhsan
Life Science Vol 8 No 2 (2019): November 2019
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v8i2.37098

Abstract

Indigenous molds from the gastrovascular cavity of nipah worms have been explored and will be applied to nipah worm cultivation in order to increase growth and production through feed. Appropriate feed formula is expected to increase the growth of worms as in their natural habitat. This study aims to explore and identify the types and characteristics of indigenous fungi from the gastrovascular tract of nipah (Namalycastis rhodochorde) which have the potential as probiotics. Isolation was carried out by pour plate method on the Potato Dextrose Agar medium. Enumeration of molds was carried out according to the Standard Plate Count rules of each sample. The selection of isolates was carried out by determining the colony character unequalities to obtain pure culture. Each pure isolate culture was coded based on the type of sample. Characterization and identification of molds was carried out based on the identification guide book by Samson: Outdoor and Indoor Fungi. The total number of mold colonies obtained from coelomal fluid, intestinal tract, and feces were 7 isolates, 7 isolates and 12 isolates respectively. The results of characterization and identification found eight groups of mold isolates from nipah worms that had similarities with members of the genus Penicillium, Aspergillus, Curvularia, Fusarium, Trichoderma, Cladosporium, and Tritirachium. Keywords: Nipah worm; molds; probiotic; Namalycastis rodhochorde, Cacing nipah; kapang; probiotik; Namalycastis rodhochorde Kapang indigenus dari saluran gastrovaskuler cacing nipah telah dieksplorasi dan akan diaplikasikan pada budidaya cacing nipah dalam rangka meningkatkan pertumbuhan dan produksi melalui pakan. Formula pakan yang tepat diharapkan mampu meningkatkan pertumbuhan cacing seperti di habitat aslinya. Penelitian ini bertujuan untuk mengeksplorasi dan mengidentifikasi jenis dan karakter fungi indigenus dari saluran gastrovaskuler cacing nipah (Namalycastis rhodochorde) yang berpotensi sebagai probiotik. Isolasi dilakukan dengan metode cawan tuang pada medium Potato Dextrose Agar. Enumerasi kapang dilakukan berdasarkan aturan Plate Count Standart dari setiap sampel. Pemilihan isolat dilakukan dengan melihat ketidaksamaan karakter koloni untuk mendapatkan kultur murni. Setiap kultur murni isolat diberikan kode berdasarkan jenis sampel. Karakterisasi dan identifikasi kapang dilakukan berdasarkan buku panduan identifikasi kapang oleh Samson: Outdoor and Indoor Fungi. Jumlah total koloni kapang yag didapatkan dari cairan coelom, saluran usus, dan feses masing-masing adalah 7 isolat, 7 isolat dan 12 isolat. Hasil karakterisasi dan identifikasi ditemukan delapan kelompok isolat kapang dari cacing nipah yang memiliki kemiripan dengan anggota genus Penicillium, Aspergillus, Curvularia, Fusarium, Trichoderma, Cladosporium, dan Tritirachium. Kata kunci: Nipah worm; molds; probiotic; Namalycastis rodhochorde, Cacing nipah; kapang; probiotik; Namalycastis rodhochorde
The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus Julia Nanda Puspita; Rikhsan Kurniatuhadi; Rahmawati Rahmawati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1700

Abstract

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).
Aktivitas Enzimatik Streptomyces spp. Yang Diisolasi Dari Usus Dan Feses Cacing Nipah (Namalycastis rhodochorde) Rikhsan Kurniatuhadi; Ari Hepi Yanti; Tri Rima Setyawati
SEMINAR NASIONAL PENINGKATAN MUTU PENDIDIKAN Vol. 1 No. 1 (2020): Seminar Nasional Peningkatan Mutu Pendidikan
Publisher : Fakultas Keguruan dan Ilmu Pendidikan Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Streptomyces terlibat dalam proses degradasi senyawa organik pada substrat pakan cacing nipah (Namalycastis rhodochorde). Streptomyces mensekresikan beberapa enzim untuk memecah makromolekul organik menjadi senyawa sederhana yang tersedia bagi detritivor cacing nipah. Penelitian ini bertujuan untuk mendeteksi aktivitas enzim enam Streptomyces spp. yang telah diisolasi sebelumnya dari usus dan feses cacing nipah. Aktivitas enzim dideteksi pada empat media yang berbeda: CMC-congo red agar, skim milk agar, gelatin nutritive agar, dan starch agar. Aktivitas katalase dideteksi menggunakan 3% larutan H2O2. Hasil penelitian menunjukkan bahwa enam Streptomyces spp. memiliki aktivitas selulolitik, amilolitik, pencairan gelatin, dan katalase. Namun hanya dua dari enam isolate Streptomyces spp yang memiliki aktivitas protease, yaitu Streptomyces NrAFA2 dan Streptomyces NrAFA4. Streptomyces NrAFA4 memiliki nilai indeks aktivitas proteolitik dan selulolitik tertinggi, yaitu 0,54 dan 1.0. sedangkan nilai indeks aktivitas selulolitik tertinggi terjadi pada isolat Steptomyces NrAFA3 yaitu 1.57. Profil aktivitas enzimatik Streptomyces spp. Yang diisolasi dari usus dan feses cacing nipah dapat dimanfaatkan untuk pengembangan formula pakan untuk budidaya cacing nipah.
ANTIFUNGAL EXTRACT ACTIVITYOF ISOLATE Nocardia sp. ATS-4.1 AGAINST Candida albicans InaCC-Y116 Abdullah Abdullah; Rahmawati Rahmawati; Rikhsan Kurniatuhadi
BERITA BIOLOGI Vol 19, No 3A (2020)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v19i3A.3868

Abstract

Candida albicans is a microorganism that knows as caused of candidiasis. Nocardia is known to have the ability to produce antifungal bioactive compounds to overcome cases of fungal infections. This research aims to determine the presence of antifungal activity and the good concentration from crude extract of Nocardia sp. ATS-4.1 to inhibit C. albicans InaCC-Y116 and. Antifungal activity test of isolate Nocardia sp. ATS-4.1 was performed using a well diffusion method on Mueller Hinton Agar (MHA) media with a concentration of 92%, 94%, 96%, 98%, 100% and nystatin (positive control) 0,0125% and DMSO 10% (negative control) incubated at 37 ᵒC for 24-48 hours. The results showed that the extract of Nocardia sp. ATS-4.1 can inhibit the growth of C. albicans. Nocardia sp. ATS-4.1 isolate extract concentration of 96% with a resistance diameter of 13.63 ± 0.53 mm with a strong category against the growth of  C. albicans InaCC-Y116.