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KARAKTERISTIK FISIOLOGIS JAMUR HALOFILIK BERDASARKAN FAKTOR LINGKUNGAN DARI SUMUR AIR ASIN DI DESA SUAK, SINTANG, KALIMANTAN BARAT rahmawati ibrahim saleh rahmawati; Lukmanul Hakim; Rikhsan Kurniatuhadi
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 2 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i2.11299

Abstract

Jamur halofilik adalah kelompok mikroorganisme yang dapat hidup dan beradaptasi pada kondisi lingkungan salin. Tujuan penelitian ini yaitu untuk mengetahui karakteristik fisiologis jamur halofilik berdasarkan faktor lingkungan dari sumur air asin di desa Suak, Kabupaten Sintang. Metode yang digunakan yaitu dengan menumbuhkan jamur halofilik pada media cair Potato dextrose broth (PDB), selanjutnya dilakukan karakterisasi fisiologis jamur. Uji fisiologis meliputi uji salinitas, suhu dan pH. Berdasarkan hasil pengamatan karakteristik fisiologis, semua isolat jamur dapat tumbuh pada salinitas 50‰ (slightly halophiles) dan 150‰ (moderate halophiles) kecuali isolat JAS8, dan semua isolat jamur tidak dapat tumbuh pada tingkat salinitas 250‰ (extreme halophiles). Semua isolat jamur dapat tumbuh pada suhu 30°C (mesofil) dan 40°C (mesofil), kecuali isolat JAS3. Hanya isolat JAS3 dan JAS7 yang dapat tumbuh pada suhu 10°C, dan semua isolat jamur dapat tumbuh pada suhu 40°C, kecuali isolat Cladosporium JAS3. Semua isolat Jamur dapat tumbuh pada pH 4 (asam) dan 7 (netral), namun isolat JAS4 dan JAS7 tidak dapat tumbuh pada pH 9, sedangkan isolat jamur yang lain dapat tumbuh pada pH tersebut. Hal ini menunjukkan bahwa jamur memiliki karakteristik yang tidak selalu sama berdasarkan faktor lingkungan. Kata Kunci: Halofilik, jamur, Sumur Air Asin, salinitas
Growth of Bacillus spp. Isolated From Nipah Worm Intestine (Namalycastis rhodochorde) With Different Combination of pH and Salinity Rikhsan Kurniatuhadi; Ari Hepi Yanti; Tri Rima Setyawati; Eka Karunia
Bioma : Jurnal Ilmiah Biologi Vol 12, No 1: April 2023
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v12i1.14387

Abstract

Bacillus spp. (NrLtF1, NrLtF5, and NrLtG2) isolated from the digestive tract of the Nypa palm worm (Namalycastis rhodochorde) were assumed to have growth characteristics according to the habitat conditions of the nypa worm. Nypa worms live in estuary environments that are affected by seawater intrusion. Salinity and pH are two environmental factors affecting gastrointestinal isolates' growth from nypa palm worms. This study aimed to determine the effect of pH and salinity on the media on growth patterns and determine the optimum combination of pH and salinity for Bacillus spp. The study was conducted using a spectrophotometry method using a microplate reader with Glucose Yeast Peptone (GYP) as a medium for bacterial growth. The medium's pH and salinity values were adjusted by adding 5M NaOH and 5M HCl to obtain pH values of 4, 5, 6, and 7, respectively. Addition of NaCl to the growing medium to get salinity values of 5%, 10%, and 15%. Optimization was determined by incubation at a density of 595 nm at a temperature of 31oC for 24 hours. The results showed that Bacillus spp. could grow well at pH 6 with a salinity value of 15%, pH 6 with a salinity value of 10%, and pH 6 with a salinity value of 5% based on contour plot design. The Optimum growth of Bacillus spp. with pH and salinity expects to be a reference for developing feed products based on indigenous nypa palm worms. 
PENINGKATAN KETERAMPILAN PEMBUATAN TEPUNG PISANG DAN BROWNIES PISANG PADA KELOMPOK PEREMPUAN PRODUKTIF DI DESA SENGKUBANG KABUPATEN MEMPAWAH: Improvement of Banana Flour and Banana Brownies Making Skills in Productive Women Group in Sengkubang Village, Mempawah District Rikhsan Kurniatuhadi; Kustiati; Siti Ifadatin; Siti Khotimah; Ari Hepi Ari Hepi Yanti; Tri Rima Setyawati; Firman Saputra; Dwi Gusmalawati; Riza Linda; Rafdinal; Elvi Rusmiyanto PW; Riyandi
JAMAS : Jurnal Abdi Masyarakat Vol. 1 No. 2 (2023)
Publisher : Forind Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (553.257 KB)

Abstract

Sengkubang Village is a mangrove area located in Mempawah Hilir District, Mempawah Regency, West Kalimantan. Sengkubang Village already has a Coastal Community Group that cares about the preservation of mangroves. However, community empowerment still needs to cover women's communities. Most women in Sengkubang Village work as housewives or help their husbands with the gardens. The implementation of community service focuses on empowering the women of Sengkubang Village by providing skills based on natural wealth and plantation products around their residences. Through this skills training, it is hoped that women will be able to fill their spare time by producing banana flour and brownies made from banana flour which are of the sale value. The method of implementing PKM is through short skills training, and participants can bring home their work. The evaluation of PKM activities was carried out using the questionnaire method at the end of the training. The training results showed the enthusiasm of the women's group from the Pelesir community in making banana flour and brownies made from banana flour. 100% of participants strongly agreed that the theme and training could develop their potential to increase their income and daily activities. Subsequent developments will include product marketing assistance through social media and e-commerce.
Antibacterial Activity of Pineapple Peel Eco-enzyme (Ananas comosus L.) on Growth Pseudomonas aeruginosa and Staphylococcus epidermidis Hendri Hendri; Zulfah Zakiah; Rikhsan Kurniatuhadi
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.5272

Abstract

Eco-enzyme is a fermented product from an organic waste substrate, molasses, and water. Eco-enzyme can be used as antibacterial because it produces organic compounds such as alcohol and acetic acid. Pineapple peel waste (Ananas comosus L.) can be used as an eco-enzyme. This study aimed to test the antibacterial activity of pineapple peel eco enzyme against Pseudomonas aeruginosa and Staphylococcus epidermidis. The study used the method of determining the Minimum Inhibitory Concentration (MIC) and Minimum Killing Concentration (MBC) of pineapple peel eco-enzyme with 8 treatment levels namely negative control (aquadest), positive control (ciprofloxacin 5 mg/mL), 3.125%, 6.25 %, 12.5%, 25%, 50%, and 100% eco-enzymes. Based on the research results, it was known that the MIC value of pineapple peel eco-enzyme against P. aeruginosa, and S. epidermidis was 12.5% and 3.125%, respectively. Eco- enzymes from pineapple peel did not show minimum killing concentrations. Eco-enzyme of pineapple peel is only bacteriostatic towards the growth of P. aeruginosa and S. epidermidis bacteria.
Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil Diah Wulandari Rousdy; Rahmawati Rahmawati; Rikhsan Kurniatuhadi
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i3.7499

Abstract

Peat soil is a type of soil that dominates the island of Borneo. Typical compounds in peat soil is humic acid. Various in vitro studies performed have shown peat subtropical humic compounds can stimulate the immune system. However, in vivo study on animal has not been done. This study aimed to determine the effect of humic acid extracted from peat soil of Borneo against the immune system, both of non-specific and specific immunity Wistar rats (Rattus novergicus). Research using a completely randomized design with five treatments and five replicates, the normal controls, a positive control (isoprinosine), humic acid 125; 250; 500 mg/kg. Humic acid was administered orally for 10 days. The results showed humic acid adduction did not significantly affect levels of hemoglobin, erythrocytes and hematocrit. Humic acid adduction of 125 mg/kg significantly affects the total leukocyte count and differential leukocyte. Humic acid 125 mg/kg also showed increased phagocytic index better than normal controls. All humic acid treatments do not provide a significant effect on the total amount of antibody. The results of this study can be used for the development of Borneo tropical peat resources as natural imunostimulant.How to CiteRousdy, D. W., Rahmawati, R. Kurniatuhadi, R. (2016). Immune Responses of Wistar Rat (Rattus novergicus) on Adduction of Humid Acid from Borneo Peat Soil. Biosaintifika: Journal of Biology Biology Education, 8(3), 401-406. 
PERANCANGAN APLIKASI TRACER STUDY UNTUK ALUMNI MASTER DAN DOCTOR UNIVERSITAS TANJUNGPURA Muthahhari, Morteza; Hadary, Ferry; Anzani, Yunita Magrima; Fahruna, Yulyanti; Asrin, Fauzan; Kurniatuhadi, Rikhsan
IJIS - Indonesian Journal On Information System Vol 8, No 2 (2023): SEPTEMBER
Publisher : POLITEKNIK SAINS DAN TEKNOLOGI WIRATAMA MALUKU UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36549/ijis.v8i2.288

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Tracer Study merupakan salah satu bentuk survey atau studi penelusuran alumni yang mempunyai nilai strategis untuk pengembangan mutu dan kualitas perguruan tinggi. Berbagai cara yang dilakukan oleh universitas untuk mengevaluasi proses pendidikan dalam rangka meningkatkan mutu pendidikan tinggi salah satunya menyelenggarakan Tracer Study. Universitas Tanjungpura dapat meluluskan sekitar lima ribu lulusan setiap tahun. Evaluasi dan pemetaan lulusan tentu saja menjadi bagian yang sangat penting bagi Universitas Tanjungpura untuk meningkatkan keberdayaan alumni di dunia kerja. Selain itu Tracer Study pascasarjana sangat memudahkan dalam pengambilan data untuk kepentingan akreditasi program studi pascasarjana (S2 dan S3) yang ada di lingkungan Universitas Tanjungpura. Pada penelitian ini dihasilkan instrumen tracer study untuk lulusan Pascasarjana. Instrumen inilah yang akan digunakan dalam perancangan aplikasi tracer study untuk alumni S2 dan S3 Universitas Tanjungpura berbasis web. Perancangan dilakukan menggunakan unified modelling language (UML) dengan metode siklus hidup pengembangan sistem untuk aplikasi. Sementara penerapan kedalam bahasa pemrograman menggunakan hypertext preprocessor (PHP). Dan hasil dari penelitian ini berupa aplikasi tracer study untuk alumni master dan doctor universitas tanjungpura.Kata Kunci: Perancangan, Tracer Study, Alumni, Universitas Tanjungpura
AKTIVITAS ANTIBAKTERI EKSTRAK METANOL Acalypha hispida TERHADAP BAKTERI Shigella flexneri DAN Bacillus cereus IHB B 379 Elvi Rusmiyanto P. Wardoyo; Devinda Ekarizky Diputri; Rikhsan Kurniatuhadi
Tengkawang : Jurnal Ilmu Kehutanan Vol 10, No 2 (2020): Tengkawang : Jurnal Ilmu Kehutanan
Publisher : Universitas Tanjungpura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26418/jt.v10i2.40187

Abstract

AbstractAcalpha hispida flower commonly used as traditional medicine. The antibacterial potential of the A. hispida flower can be used to treat diarrhea and cancer. The purpose of this study was to determine the antibacterial activity and effective concentration of methanol extract of A. hispida flower against to S. flexneri and B. cereus. Sample used was flower of A. hispida from Kota Pontianak. The solvent used is metahnol. Antibacterial activity test using the disc diffusion method (Kirby-Bauer) with parameters based on grading levels  extract concentration in S. flexneri was 1.2;  1.3;  1.4;  1.5;  and 1.6 g/ml and B. cereus for 0.75;  0.80;  0.85;  0.90;  and 0.95 g/mL, 10% DMSO and ciprofloaxin as a comparison. the results of the research showed that the concentration of methanol extract of A. hispida flower which can effectively inhibit the growth of S. flexneri and B. cereus bacteria at 1.2 g / mL and 0.75 g / mL. The methanol extract of A. hispida flower contains compounds of alkaloids, flavonoids, tannins, phenols, steroids and terpenoids.Keywords : Acalypha hispida, Antibacterial, Shigella flexneri, Bacillus cereus AbstrakAcalypha hispida merupakan tanaman obat trardisional yang sudah dimanfaatkan masyarakat secara turun temurun untuk mengobati diare dan kanker. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri dan konsentrasi efektif ekstrak metanol bunga A. hispida terhadap bakteri Shigella flexneri dan Bacillus cereus. Sampel penelitian yang digunakan adalah bunga A. hispida yang diperoleh dari Kota Pontianak. Pelarut yang digunakan adalah methanol. Uji aktivitas antibakteri menggunakan metode difusi cakram (Kirby-Bauer) dengan perlakuan 5 taraf konsentrasi ekstrak pada bakteri S. flexneri sebesar 1,2; 1,3; 1,4; 1,5; dan 1,6 g/ml serta pada bakteri B. cereus IHB B 379 sebesar 0,75; 0,80; 0,85; 0,90; dan 0,95 g/mL, DMSO 10% serta sebagai pembanding digunakan ciprofloaxin. Hasil penelitian menunjukkan bahwa konsentrasi ekstrak methanol bunga A. hispida yang secara efektif dapat menghambat pertumbuhan bakteri S. flexneri dan B. cereus sebesar 1,2 g/mL dan 0,75 g/mL. Ekstrak methanol bunga A. hispida mengandung senyawa golongan alkaloid, flavonoid, tannin, fenol, steroid dan terpenoid.Kata kunci: Acalypha hispida, antibakteri, Shigella flexneri, Bacillus cereus
The Composition of Arthropods in Nipah Fronds Decomposition at Sungai Kakap Mangrove Area in the West Kalimantan Lastyanti Mulyani; Junardi; Rikhsan Kurniatuhadi
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i4b.5718

Abstract

Anthropogenic activities in Nypa palm (Nypa fructicans) forests directly leave a lot of residual nipa fronds, which take longer to decompose. The decomposition process involves decomposer organisms, one of which is Arthropods. Nevertheless, information regarding the composition of decomposition fronds is still limited. This study aims to obtain data and information regarding the composition of Arthropods and their relationship to the decomposition period. Arthropod sampling was carried out by hand collecting methods at 15, 25, 35, and 45 days after placement. The laying of the nipa palm fronds was determined randomly with a vertical position at 20 cm above the ground surface. Arthropod eggs, larvae, pupae, and imago samples were sorted and preserved in 70% Alcohol. Arthropod data obtained were analyzed descriptively. The results showed that the composition of Arthropods consisted of the class Crustacea and Insecta, each with one order (2 genera) and six orders (32 genera). The orders Diptera (Psychoda, Eristalinus) and Amphipoda (Allorchestoides) were dominant. The average number of individuals found increases with the decomposition period. The role of different arthropods influences their composition in the decomposition process of the nipa palm fronds. It is necessary to study the composition of microbes in each decomposition period.
Aktivitas Antifungi Ekstrak Metanol Sarcotheca diversifolia (Miq) Hallier F terhadap Pertumbuhan Candida albicans Yuliati; Kurniatuhadi, Rikhsan; Khotimah, Siti
SCISCITATIO Vol. 5 No. 2 (2024): Volume 5 Number 2, January 2024 (Online First)
Publisher : Universitas Kristen Duta Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21460/sciscitatio.2024.52.180

Abstract

Asam Kalimbawan (Sarcotheca diversifolia (Miq) Hallier F.) merupakan tumbuhan liar yang berpotensi sebagai tanaman obat dan diketahui  sebagai antioksidan. Tujuan penelitian ini untuk mengetahui potensi antifungi ekstrak metanol buah Sarcotheca diversifolia terhadap Candida albicans. Ekstraksi buah asam kalimbawan (S. diversifolia) dilakukan dengan metode maserasi pada pelarut metanol pro analis. Pengujian antifungi menggunakan metode difusi lubang sumuran di media Saboraud Dextrose Agar. Konsentrasi ekstrak metanol buah S. diversifolia yang diujikan adalah 0,2; 0,4; 0,6; dan 0,8 g/ml, kontrol positif ketokonazol 2%, dan kontrol negatif DMSO 10%. Hasil penelitian memperlihatkan bahwa ekstrak metanol buah asam kalimbawan (S. divesifolia) dapat menghambat pertumbuhan C. albicans melalui pembentukan zona bening di sekitar sumuran. Daya hambat ekstrak metanol buah asam kalimbawan pada ketiga konsentrasi termasuk dalam kategori sedang dengan sifat daya hambat fungistatik. Kekuatan daya hambat dari ekstrak metanol buah asam kalimbawan tersebut memiliki 29-28% dibandingkan dengan daya hambat 2% ketokonazol dengan kategori kuat. Semua konsentrasi uji bersifat fungistatik di jam ke 48. Metabolit sekunder yang terdeteksi pada ekstrak metanol buah S. diversifolia terdiri atas alkaloid, flavonoid, steroid, terpenoid, saponin, dan tanin. Buah asam kalimbawan (S. divesifolia) diduga berpotensi sebagai fungistatik berbasis bahan alam.
The Chronology of Development Tracer Study System at Tanjungpura University Kurniatuhadi, Rikhsan; Hadary, Ferry; Sulistyarini, Sulistyarini; Anzani, Yunita Magrima; Fahruna, Yulyanti; Muthahhari, Morthezza
Jurnal Teknologi Informasi dan Pendidikan Vol. 17 No. 1 (2024): Jurnal Teknologi Informasi dan Pendidikan
Publisher : Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/jtip.v17i1.746

Abstract

The implementation of the Tracer Study at Tanjungpura University has been carried out since 2011 and became an electronic or online system in 2015. The system and design of the online tracer study at Tanjungpura University experienced dynamics that were adjusted to internal and external factors from 2015 to 2022. The influencing factors included changes in the mandate Dikti/Belmawa, resources, and the development of tracer study instruments. These factors cause several obstacles and challenges in creating an efficient Tracer Study system and desain. The purpose of As discussion is to review an overview of the development of the Tracer Study system and design at the Tanjungpura University to create a more efficient Tracer Study system. The research method is carried out by identifying a system model developed from 2015 to 2022 with the Borg and Gall models. The search results show that there have been system and design changes four times from 2015 to 2022. Furthermore, some systems and designs are less efficient in implementing the tracer study from 2015 to 2019 by making an online surveyor input system to reduce the tracer study's essence and function. However, in 2020, the online filling process has all been directed by alumni to fill in directly.