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All Journal Jurnal Bios Logos Protobiont: Jurnal Elektronik Biologi Tengkawang : Jurnal Ilmu Kehutanan AL KAUNIYAH BERITA BIOLOGI BIOMA BIOMA : Jurnal Biologi Makassar Jurnal Biologi Tropis IJIS - Indonesian Journal On Information System JURNAL PENGABDI Biosaintifika: Journal of Biology & Biology Education INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Teknologi Informasi dan Pendidikan Agroprimatech Biologica Samudra Sciscitatio : Journal for Biological Science Jurnal Borneo Akcaya : Jurnal Penelitian dan Pengembangan Pelayanan Publik Bioscientist : Jurnal Ilmiah Biologi Seminar Nasional Peningkatan Mutu Pendidikan Life Science JAMAS: Jurnal Abdi Masyarakat Life Science Berita Biologi
Rikhsan Kurniatuhadi, Rikhsan
Department Of Biology, Faculty Of Mathematics And Natural Sciences, Universitas Tanjungpura, Pontianak, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Inventarisasi Khamir pada Ragi Tape yang Terbuat dari Tepung Beras (Oryza sativa L.) Varietas Lokal Daniati, Eleutra; Kurniatuhadi, Rikhsan; Turnip, Masnur
JURNAL BIOLOGICA SAMUDRA Vol 5 No 2 (2023): Biologica Samudra
Publisher : Program Studi Biologi, Fakultas Teknik, Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33059/jbs.v5i2.6141

Abstract

White rice flour (Oryza sativa L.) is the main ingredient used by the Dayak community in the manufacture of tape yeast, the tape yeast is one of the substrates for the growth of microorganisms, one of which is yeast. Yeast belongs to the group of fungi that are unicellular in shape and have been isolated from various kinds of traditional fermented foods that are consumed such as tape. The purpose of this study was to determine the genus of yeast isolated from tape yeast made from white rice flour. This research was conducted in December 2021 – April 2022. Yeast isolates were identified based on macroscopic and microscopic observations of colonies from yeast cells, cell reproduction, and physiological tests with reference to the book The Yeast a Taxonomic Study. Based on the results of this study, 12 yeast isolates were obtained consisting of three genera, namely Kluyveromyces, Pichia and Saccharomyces.
Antibacterial Activity of Simpur Leaves Methanol Extract (Dillenia sp.) Againts Staphylococcus aureus Shafira Qurrata A'yuni; Rikhsan Kurniatuhadi; Zulfa Zakiah
JURNAL BIOS LOGOS Vol. 15 No. 1 (2025): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v15i1.54604

Abstract

Simpur (Dillenia sp.) is a plant that can be used as traditional antibacterial medicine. This study aims to determine the inhibitory activity of Staphylococcus aureus bacteria when exposed to a concentration of methanol extract from Simpur leaves (Dillenia sp.). This study utilized a completely randomized design with five concentration levels of Dillenia sp. specifically, the study included a control group and methanol extracts of Simpur leaves at concentrations of 5%, 10%, 15%, and 20%. The methods used included total plate count, hemocytometer, and UV-Vis spectrophotometry, which were then analyzed using ANOVA (Analysis of Variance). The results showed that the greatest inhibition occurred in the methanol extract of Simpur leaves (Dillenia sp.) at a concentration of 20%, there was a decrease in the number of colonies ranging from 90% to 94%, while the decrease in the number of cells ranged from 17% to 18%. The lowest inhibition was found in the methanol extract of Simpur leaves (Dillenia sp.) with a concentration of 10%, the proportion of decreasing the number of colonies ranged from 63% to 70%, while the proportion of decreasing the number of cells ranged from 6% to 7%. The highest optical density (OD) value at a 20% concentration ranged from 0.482 to 0.547, while the lowest value at a 5% concentration ranged from 0.127 to 0.131. The conclusion of this study is that the methanol extract of Simpur leaves (Dillenia sp.) Bacteriostatic antibiotics only inhibit bacterial growth and cannot kill bacteria.
GROWTH OF NYPA PALM WORMS (Namalycastis rhodochorde) JUVENILES FED ON NYPA PALM FRONDS FERMENTED BY CELLULOLYTIC BACTERIA NrLtC4 AND NrLtG2 Hosiana Patricia Sheren; Ari Hepi Yanti; Tri Rima Setyawati; Rikhsan Kurniatuhadi
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11352

Abstract

Nypa palm worms (Namalycastis rhodochorde: Polychaetes) play important ecological roles as detritivores of organic matter in mangrove ecosystems, bioindicators of water pollution, and sediment stabilizers in estuarine environments. N. rhodochorde also shows potential for use in fermented feed-based aquaculture due to its high nutritional value as a natural food source for aquatic organisms. Cellulolytic lactic acid bacteria isolated from the coelomic fluid and gastrointestinal tract of N. rhodochorde, identified as isolates NrLtC4 and NrLtG2, have potential as starter cultures for fermenting niypa palm fronds, which are used as feed in nypa palm worm cultivation. This study aimed to evaluate the growth of juvenile N. rhodochorde fed nypa palm fronds fermented by the cellulolytic bacteria NrLtC4 and NrLtG2. Juveniles were maintained under consistent stocking density, container size, and water volume, with three treatments arranged in a completely randomized design: unfermented nypa palm fronds (control), nypa palm fronds fermented with isolate NrLtC4, and nypa palm fronds fermented with isolate NrLtG2. The results demonstrated that fermented feed significantly influenced both the number of body segments [p = 0.00021 and 0.0028 (p < 0.05)] and body length [p = 0.0020 and 0.0035 (p < 0.05)] of the juveniles. Juveniles fed nypa palm fronds fermented with strain NrLtC4 showed increases of 29.6% in segment number and 58.5% in body length, while those fed fronds fermented with strain NrLtG2 exhibited increases of 36.6% and 63.9%, respectively. The highest juvenile survival rate was observed in the NrLtC4 fermented feed group at 72%, followed by 69.33% in the NrLtG2 group. These findings suggest that bacterial isolates NrLtC4 and NrLtG2 have promising potential as a consortium feed to enhance the growth of nypa palm worms in aquaculture.
The Antibacterial Activity of Thermoactinomyces sp. (H24) Extract Against Escherichia coli and Staphylococcus aureus Julia Nanda Puspita; Rikhsan Kurniatuhadi; Rahmawati Rahmawati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 3 No 1 (2021): Laboratory innovation : The challenge for medical laboratory
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v3i1.1700

Abstract

Bacteria of the genus Thermoactinomyces have the ability to produce antibacterial bioactive compounds. This bioactive compound can be used for combating diarrheal agents such as Escherichia coli and Staphylococcus aureus. This study aims to determine the antibacterial activity of the metabolite extract from Thermoactinomyces sp. (H24) against Escherichia coli and Staphylococcus aureus. Methanol was used as a solvent for the extraction of bacterial bioactive compounds. Antibacterial activity was analyzed by the diffusion method with several extract concentrations (0.75 mL, 1.5 mL, 2.25 mL, and 3 mL), 10% DMSO as the negative control, and ciprofloxacin as the positive control. Our result shows that Termoactinomyces sp. (H24) extract has an inhibitory effect on the growth of  E. coli and S. aureus with an effective concentration of 2.25 mL (inhibition strength: very strong).
Pathogenicity Characteristics of Front, Middle, and Hind Nipah Worm (Namalycastis rhodochorde) Digestive Tract Bacteria Dian Indrawati; Ari Hepi Yanti; Rikhsan Kurniatuhadi
Jurnal Biologi Tropis Vol. 26 No. 3 (2026): Juli - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i3.12427

Abstract

Nipah worms (Namalycastis rhodochorde) are one of Indonesia's invertebrate species used as high-protein aquaculture feed. The presence of pathogenic bacteria is a factor in the decline in quality and quantity in nipah worm cultivation, especially bacteria originating from the digestive tract of nipah worms. This study was conducted to examine the characteristics of pathogenicity of bacteria originating from the digestive tract of Nipah worms in the form of hemolysis and DNase activity. The hemolysis and DNase activity tests were performed using the streak plate method on Blood Agar Plate (BAP) and DNase Agar media. The results obtained in the hemolysis test showed a positive reaction in 68 isolates (90.7%), consisting of 5 α-hemolysis isolates, 63 β-hemolysis isolates, and 7 γ-hemolysis isolates. β hemolysis or complete hemolysis was found in 27 isolates in the foregut, 18 isolates in the midgut, and 18 isolates in the hindgut. The results obtained in the DNase activity test were positive in 17 isolates (22.7%), consisting of 9 isolates from the front digestive tract, 1 isolate from the middle digestive tract, and 7 isolates from the rear digestive tract. This study concludes that the high levels of hemolysis and DNase activity in bacterial isolates from the digestive tract of the nipah worm are predictors of pathogenicity. Bacterial isolates from the foregut showed the highest pathogenic activity, which is thought to be due to the presence of bacteria carried during the feeding process of the nipah worm.
Co-Authors Abdullah Abdullah Apriani, Lisa Ari Hepi Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Ari Hepi Yanti Asrin, Fauzan Daniati, Eleutra Devinda Ekarizky Diputri Diah Wulandari Rousdy Dian Indrawati Dwi Gusmalawati Edi Kurniadi Eka Karunia Elvi Rusmiyanto Pancaning Wardoyo, Suci Lestari, Mukarlina, Fahruna, Yulyanti Ferry Hadary Firman Saputra Firman Saputra Harfan, Donatus Tia Hendri Hendri Henno Wisnu Putra Holyness Nurdin Singadimedja Hosiana Patricia Sheren Indah Permatasari Indah Pratiwi Irwan Lovadi Ita Purnama Sari, Ita Purnama Julia Nanda Puspita Junardi Junardi Junardi Komara, Dina Kustiati Kustiati Kustiati Larasaty, Suprih Lastyanti Mulyani Lukmanul Hakim Muhammad Luthfi Mukarlina, Mukarlina Mulyani, Lastyanti Muthahhari, Morteza Muthahhari, Morthezza Naulandari, Mesi Rafdinal Rafdinal Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati Rahmawati, Rahmawati Riyandi Riyandi Riyandi Riyandi Riza Linda Riza Linda selno, Silvester Shafira Qurrata A'yuni Siti Ifadatin Siti Ifadatin Siti Khotimah Sulistrioningsih, Sulistrioningsih Sulistyarini, Sulistyarini Tri Rima Setyawati Tri Rima Setyawati Tri Rima Setyawati Tri Rima Setyawati Turnip, Masnur Williem, Albertus Yunita Magrima Anzani Zulfa Zakiah Zulfa Zakiah