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All Journal JURNAL GALUNG TROPIKA Pendas : Jurnah Ilmiah Pendidikan Dasar JURNAL PENDIDIKAN TAMBUSAI Jurnal Ilmiah Mandala Education (JIME) Jurnal Pengabdian dan Peningkatan Mutu Masyarakat (JANAYU) MALLOMO: Journal of Community Service JAGO TOLIS : Jurnal Agrokompleks Tolis JURNAL SAINS DAN TEKNOLOGI PERIKANAN (JIKAN) Indonesian Journal of Educational Development (IJED) Hippocampus: Jurnal Pengabdian Kepada Masyarakat Depik Jurnal Ilmu-Ilmu Perairan, Pesisir dan Perikanan Hayyan Journal Pedagogy: Journal of Multidisciplinary Education PENDIMAS JMMU: Jurnal Mahasiswa Manajemen dan Umum Genbinesia Journal of Biology
Nurul Mutmainnah, Nurul
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Journal : Hayyan Journal

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Utilization of Yam Extract as an Alternative to Crystal Violet for Staining Streptococcus mutans Bacteria Ridwan, Asriyani; Fatimah; Mutmainnah, Nurul; Faisal
Hayyan Journal Vol. 2 No. 3 (2025): October
Publisher : Education and Talent Development Center of Indonesia (ETDC Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51574/hayyan.v2i3.4328

Abstract

Gram staining is a fundamental technique in microbiology, vital for the initial identification of bacteria based on differences in cell wall structure, classifying them as Gram-positive and Gram-negative. This method is crucial for rapid clinical diagnosis. Currently, its primary stain, Crystal Violet, raises serious concerns due to its mutagenic, toxic, and environmentally unfriendly properties, necessitating the search for safer and more sustainable alternatives. This study focuses on the potential of yam tubers (Dioscorea sp.), an abundant local resource, as a substitute for Crystal Violet. Yam tubers are rich in anthocyanins, natural pigments with cationic properties and antibacterial activity, making them a promising dye candidate. The selected test bacterium was Streptococcus mutans, a Gram-positive, coccus-shaped bacterium and a major cause of dental caries, ideal for validating the staining ability of thick cell walls. The aim of this study was to evaluate the effectiveness of yam tuber extract in staining S. mutans and to compare its staining quality with standard Crystal Violet. The expected outcome is the creation of an economical, environmentally friendly, and sustainable staining method.
Co-Authors Alimin Alwi Andi Adam Malik Andi Dita Tawakkal Gau Ashaq, Muhammad Hud Bariyyah, Sitti Khairul Dewi, Azizah Kusuma Ernawati Ernawati Fachry Abda El Rahman Faisal Fatimah Fauziah Fauziah Fitri Indah Yani, Fitri Indah Fitri Sil Valen Fitri, Fitri Indah Yani HARMILAWATI Hartina, Wanda Hidayatullah Ilham, Batara Indirwan, Indirwan Islamy, R Adharyan Johan Mahyudi, Johan Kadir, Muhammad Jurhadi Lidya Lidya, Lidya Mayasari Yamin, Mayasari Melati Amir, Rahma MMSI Irfan ,S. Kom Muh. Kasim Muhamad Ikbal Najamuddin Najamuddin, Najamuddin Neti S, Neti S Novalina Serdiati Nur Maida, Andi Nur, A. Muafiah Nurauralfiani, Putri Nurfadilla RANGGA ADITYA Ratna Wardhani reski aulia RR. Ella Evrita Hestiandari Rusdi, Rismawaty S, Aswandikari Sabaruddin Sabaruddin Sahabuddin SAHABUDDIN SAHABUDDIN Shabrina Shabrina, Shabrina Sirajuddin Saleh Sri Nur Qadri Suherman, Suherman Syamsiar Zamzam Ummu Kaltsum Ummu Kaltsum SC Veryl Hasan Warda Susaniati Yuni Kilawati Yunus, Yusdalifa Ekayanti Yunus, Yusdalifah Eka Yanti Yushra, Yushra Zulfiani