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A. M. Muslihin
Universitas Pendidikan Muhammadiyah Sorong
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Veterinary Other

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PERBANDINGAN METODE EKSTRAKSI TERHADAP KADAR TOTAL FLAVONOID DAN ALKALOID DAUN BATIK PAPUA (Graptophyllum pictum L. griff) Nadziatul Khasanah; A.M. Muslihin
JURNAL ETNOFARMASI Vol. 3 No. 01 (2025): JURNAL ETNOFARMASI
Publisher : Universitas Pendidikan Muhammadiyah Sorong

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36232/jurnalfarmasiunimuda.v3i01.1964

Abstract

One of the plants used as medicine by the community is the Papuan batik leaf (Graptophyllum pictum L. Griff). Empirically, this leaf is used to treat anemia, treat wounds, liver swelling, gallstones, and treat coughs. This study aims to determine the results of the extract yield based on differences in cold extraction methods (maceration) and hot methods (soxhletation and reflux) and to identify functional groups contained in plants using the FTIR spectroscopy method and to see differences in total alkaloid and flavonoid compound levels based on differences in extraction methods using the UV-Vis spectrophotometry method. The results of the research that has been carried out indicate that there is the most effective extraction method used, namely the soxhletation method with an extract weight of 14 g and a yield percentage of 28%. Phytochemical screening test of Papua batik leaf ethanol extract using maceration, soxhletation, and reflux methods proved to contain alkaloid, flavonoid, tannin, terpenoid, and steroid compounds, also proven by the results of FTIR test with the presence of N-H, C=C, C-H, C-N, and C-O functional groups in the extract. The highest flavonoid content in the maceration method was 322.414 mg/g extract and the highest alkaloid content in the maceration method was 144.6 mg/g extract.
Studi Fitokimia Senyawa Aktif Ekstrak Batang Nelambo suon (Rubiaceae) Rossardy, Marzella Dea; Muslihin, A.M.; Watora, Wahyuni; Budiyanto, Angga Bayu; Hardia, Lukman; Irwandi, Irwandi; Aponno, Alyssa Mileta; Utami, Hikmahtul Putri
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1333

Abstract

Background: Papua has high biodiversity with significant potential as a source of traditional medicinal plants, including Nelambo suon (Rubiaceae), which has been empirically used by local communities. However, scientific information regarding the active compounds in its stem bark remains limited. Objective: This study aimed to identify the secondary metabolite groups present in the ethanol extract of Nelambo suon stem bark through phytochemical screening. Methods: The research was conducted experimentally using the maceration method with 80% ethanol as the solvent. The stem bark simplicia was extracted and qualitatively screened for alkaloids, flavonoids, tannins, and saponins using specific reagents. Results: The results showed that the ethanol extract of Nelambo suon stem bark contained alkaloids, flavonoids, tannins, and saponins, indicated by characteristic color changes, precipitate formation, and foam production. Conclusion: These findings suggest that Nelambo suon stem bark is a potential source of bioactive compounds and may be further developed as a herbal medicinal material based on local wisdom.
Antioxidant The Effect of Solvent Variation on Antioxidant Activity in Red Fruit Extract (Pandanus Conoideus Lam) Using UV-Visible Spectrophotometry Liwen Vinesia Bilat Werluka; Ratih Arum Astuti; AM Muslihin
Jurnal EduHealth Vol. 17 No. 01 (2026): Jurnal EduHealt, January-March 2026
Publisher : Sean Institute

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Abstract

This study aims to determine the effect of different solvents on the antioxidant activity of red fruit seed extract (Pandanus conoideus Lam). It was analysed using the DPPH assay and UV–Vis spectrophotometry. Samples were extracted using the maceration method with various solvents, namely 96% ethanol, n-hexane, and ethyl acetate. The yield results showed that ethanol, n-hexane, and ethyl acetate extracts yielded 18%, 12%, and 11%, respectively. Phytochemical screening tests showed that ethanol extracts contained flavonoids, alkaloids, tannins, and saponins. The n-hexane extract contained alkaloids, while the ethyl acetate extract contained alkaloids and saponins. Antioxidant activity was assessed based on the IC₅₀ value of the ethanol extract, which showed antioxidant activity with an IC₅₀ value of 54.67 μg/mL, categorised as a very strong antioxidant. The ethyl acetate extract had an IC₅₀ value of 19.92 μg/mL, categorised as strong, while the n-hexane extract showed the highest IC₅₀ of 9.40 μg/mL, categorised as very strong. These differences indicate that solvent polarity affects the amount and type of bioactive compounds extracted, thereby affecting the ability to capture free radicals. Thus, these results indicate that n-hexane solvent provides the strongest antioxidant activity in red fruit seed extractsc.
Co-Authors Aisyah, Heti Angga Bayu Budianto Angga Bayu Budiyanto Aponno, Alyssa Mileta Astuti, Ratih Arum Darmayanti Tumpu Dieta Andaristi Erawati, Rika Fabanyo, Syahrul H Fabanyo, Syahrul H. Fadhilah, Windi Nur Fahira, Ifana Nur Hardiansyah, La Ode Heti Aisyah Irwandi , Irwandi Irwandi Irwandi Irwandi Irwandi Irwandi Irwandi Khusnul Fitriana Liwen Vinesia Bilat Werluka Lukman Hardia Maria Gloria Yeuyanan Maulana, Fajar Muhammad Andy Irfan Mustopa Mustopa Nadziatul Khasanah Ni'ma, Eka Savira Nur Hasana Lamarunga Nur ‘Azah nurjanah, sartika wahyu Pratama, Agus Sangka Ratih Arum Astuti Rossardy, Marzella Dea Selfiana Iek Serlin Marsela Sintya Sari Dwi Kusumaningrum Tunazzila, Nurhikmah Utami, Hikmahtul Putri Wa Ode Nurwahida Wasaraka, Fadila Alfiah Watora, Wahyuni Yapsenang, Amar Jansen