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Kuncara, Rachmad Bayu
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Dentistry Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health

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Derajat Aglutinasi pada Pemeriksaan Golongan Darah Metode Tabung Berdasarkan Masa Simpan Test Sel A dan Test Sel B Hari Ke-0, Ke-2, Ke-4, Ke-6 dan Ke-8 Zatalini, Karinta Syifa; Kuncara, Rachmad Bayu; Sugihantono, Anung
Jaringan Laboratorium Medis Vol. 6 No. 2 (2024): November 2024
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v6i2.11733

Abstract

Blood group examination is an examination that aims to determine the type of blood group. Cell test is a blood group examination reagent used to detect antibodies in the serum being examined. The long shelf life of cell tests that can only last for two days is considered less effective for agencies with a high level of blood services. The purpose of this study was to describe the degree of agglutination in blood group examination with cell test A and B stored on the 0th, 2nd, 4th, 6th, and 8th days. This study is a descriptive study with a Quasi Experimental research design. Test cell A and test cell B were made from red blood cell specimens of 3 blood type A and 3 blood type B respectively. Test cells are stored in a refrigerator with a temperature of 2-6° C. Test cells were then examined on the 0th, 2nd, 4th, and 6th day of storage. Calculation of samples and repetitions using the Federer formula with the number of treatments in this study is 5 treatments. Based on the calculation, one sample of test cell A and test cell B was obtained with five repetitions of each examination. The results showed that on the 0th, 2nd, 4th, and 6th day of cell test storage, the results of blood type examination were obtained, namely the degree of agglutination 4+ with erythrocytes in the cell test clumping into one bond, cells forming large agglutination with clear supernatant. On the 8th day of storage, the result of agglutination degree is 3+ with erythrocytes in test cells not clumping perfectly, there are erythrocyte granules and cloudy supernatant. Based on the results of the study, it is concluded that test cell A and test cell B can be used optimally until day 6 storage.
Perbedaan Kadar TNF-α pada Penderita Tuberkulosis Paru Sebelum dan Sesudah Satu Bulan Terapi Obat Anti Tuberkulosis Kuncara, Rachmad Bayu; Purlinda, Devi Etivia; Setyaji, Yoki; Sulistyasmi, Wiwit
Jaringan Laboratorium Medis Vol. 7 No. 1 (2025): May 2025
Publisher : Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jlm.v7i1.12789

Abstract

Pulmonary tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Patients diagnosed with pulmonary tuberculosis undergo Anti-Tuberculosis Drug (OAT) therapy for six months. In the first two months, known as the intensive phase, patients receive a combination of OATs such as isoniazid, rifampicin, ethambutol, and pyrazinamide. TNF-α is a pro-inflammatory cytokine that plays a crucial role in granuloma formation to contain the spread of Mycobacterium tuberculosis. This study aims to determine the difference in TNF-α levels in pulmonary tuberculosis patients before and after one month of OAT therapy. The study used an observational analytic design with a prospective cohort approach without comparison. A total of 17 newly diagnosed pulmonary tuberculosis patients at Bangetayu, Kedungmundu, and Tlogosari Wetan Health Centers, Semarang City, were included. TNF-α levels in serum samples were measured using the ELISA method. The mean TNF-α level before OAT therapy was 110.09±100,48 pg/mL, while after one month of therapy, it decreased to 90,05±60,12 pg/mL. The statistical analysis using the Mann-Whitney test yielded a p-value of 0.228 (p > 0.05), indicating that there was no statistically significant difference in TNF-α levels before and after one month of anti-tuberculosis drug (OAT) therapy. However, the mean TNF-α levels showed a decreasing trend, which, although not statistically significant, may suggest an initial immunological response to the treatment. The researcher recommends further studies with a larger sample size and longer observation period—such as until the end of the intensive phase or the completion of therapy—in order to obtain more representative and statistically significant results regarding TNF-α dynamics during tuberculosis treatment.
Education and Training Empowerment for Posbindu Cadres to Prevent Non-Communicable Diseases in Sendangmulyo Village, Semarang City Qomariah, Nurul; Widiyanto, SY. Didik; Wikandari, Ririh Jatmi; Kuncara, Rachmad Bayu
Jurnal Ilmiah Pengabdian Masyarakat Bidang Kesehatan (Abdigermas) Vol. 2 No. 2 (2024): Jurnal Ilmiah Pengabdian Kepada Masyarakat Bidang Kesehatan (Abdigermas)
Publisher : CV Media Inti Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58723/abdigermas.v2i2.166

Abstract

The purpose of this PPDM is to strengthen the cadre of Posbindu PTM officers in 3 RW of Sendangmulyo village in order to provide optimal health services and how to increase awareness of their own health and family, so as to reduce the number of NCD patients. The targets of PPDM are cadres of Posbindu PTM Officers in RW 15, 20 and 24 Sendangmulyo urban villages, Tembalang sub-district, Semarang City. The method used is education, training 15 cadres of Posbindu PTM officers, to be implemented during Posbindu PTM in their RW routinely in the form of health counseling) and providing services for checking blood pressure, weight, height, abdominal circumference, cholesterol, uric acid and blood sugar, as well as consulting services about complaints felt by residents, then conducted monitoring and evaluation by pengabdi related to improving the quality of health services carried out by cadres of Posbindu PTM officers after being trained. The results obtained are that residents feel more able to know their health conditions so that they can improve their health conditions on the basis of direction, advice from officers at table 5 and the quality of service is getting faster, dexterous, and looks skilled in the use of tools.  
Co-Authors Anung Sugihantono Auliya, Qurrotu A’yuni Azahra, Sresta Djoko Priyatno, Djoko Duri, Iin Desmiany Fuzianingsih, Eka Noviya Hadipranoto, Ichsan Istiqomah, Anisa Kasiyati, Menik Najiah, Dewina Zumrotin Nastiti, Hilari Rio Rosa Nurul Qomariah Nurul Qomariyah Purlinda, Devi Etivia Putri, Adita Puspitasari Swastya Ririh Jatmi Wikandari Rodhi Hartono Roni Afriansya Sasangka, Parisade Galih Setyaji, Yoki Widiyanto, SY. Didik Wiwit Sulistyasmi Zatalini, Karinta Syifa