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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Dasar Indonesian Journal of Cancer Chemoprevention AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology The Indonesian Biomedical Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Biologi Tropis JSFK (Jurnal Sains Farmasi & Klinis) Menara Perkebunan Advances in Tropical Biodiversity and Environmental Sciences Proceeding Biology Education Conference Berkala Ilmiah Biologi
Tri Rini Nuringtyas
Department Of Biology, Faculty Of Biology, Universitas Gadjah Mada, Jl. Bulaksumur, Sleman, DI Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Veterinary

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Identifikasi Senyawa Curcumin, Bisdemethoxycurcumin dan Demethoxycurcumin Rimpang Curcuma xanthorrhiza Roxb. dan Curcuma longa L. dengan 1H NMR Erwin Nur Indiarto; Tri Rini Nuringtyas; Yekti Asih Purwestri; Respati Tri Swasono; Yosi Bayu Murti
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 15, No 1 (2018): Proceeding Biology Education Conference
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Curcumin and its derivates (Bisdemethoxycurcumin, Demethoxycurcumin) are natural dyes found in Curcuma xanthorrhiza dan Curcuma longa. Parmacologic evidence suggests all three are antioxidant, antiinflamatory, and anticancer. The objective of this research was to identify and measure the intensity of Curcumin compounds, Bisdemethoxycurcumin, and Demethoxycurcumin rhizome C. xanthorrhiza and C. longa by using 1H NMR spectra. Rhizome C.xanthorrhiza and C. longa collected from Wonogiri, Central Java. The rhizomes were dried in the oven at 40 °C and were powdered using a blender. The dried powder sample was extracted using methanol-d4  containing 0.01% TMSP and followed by ultrasonication for 15 minutes. The supernatant was separated from the solution by 10.000 rpm centrifugation for 10 minutes. The supernatant was transferred to NMR tube and analysed by using JEOL 500 MHz NMR. The obtained data were analysed by Mnova 12 software and Simca 14. Results of comparsion of 1H NMR spectra of both rhizomes with reference were identified Curcumin, Bisdemethoxycurcumin, Demethoxycurcumin compounds. The highest intensity all of three compound was found in Curcuma longa and with One way Anova test result obtained p<0,05. The two rhizomes show a good separation on the OPLS-DA scoreplot with Q2 82,1% indicating a good model. The study showed that Curcumin, Bisdemethoxycurcumin, Demethoxycurcumin compounds were found in both rhizomes and had different intensity
SPAD-502 and atLEAF CHL PLUS values provide good estimation of the chlorophyll content for Hevea brasiliensis Müll. Arg. Leaves Andi Nur CAHYO; Rudi Hari MURTI; Eka Tarwaca Susila PUTRA; Tri Rini NURINGTYAS; Denis FABRE; Pascal MONTORO
E-Journal Menara Perkebunan Vol 88, No 1 (2020): April, 2020
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.369

Abstract

Measurement of chlorophyll content using destructive methods is not efficient due to a large number of samples, cost, and time needed. Estimationof chlorophyll content by nondestructive methods using handheld chlorophyll meter may be considered to improve efficiency. This research aimed to determine the formula to convert SPAD-502 and atLEAF CHL PLUS values (relative indicator of chlorophyll content) to estimated (absolute) rubber leaves chlorophyll content. Twenty leaves of rubber plant were measured using SPAD-502 and atLEAF CHL PLUS at the same time to determine SPAD-502 and atLEAF CHL PLUS values. The measured leaves were then collected to determine the chlorophyll content using a standard laboratory procedure. Regression and correlation analyses (among 3 methods) were conducted using SAS v.9 software. The results showed that between SPAD-502 and atLEAF CHL PLUS values were closely correlated, hence both of the devices can substitute each other to estimate rubber leaf chlorophyll content. In addition, the relationship between atLEAF CHL PLUS and SPAD-502 values with actual chlorophyll content of rubber clone SP 217, PB 260, GT1, and all clones (general) were significant with high coefficient of determination (R2) as well as low Root Mean Square Error (RMSE) and Coefficient of Variation (CV). Therefore, by using formula determined in this study, both atLEAF CHL PLUS and SPAD-502 can be suggested for accurate, fast, and non-destructive estimation of chlorophyll content of rubber plant leaf.
Color Stability of Phycoerythrin Crude Extract (PECE) from Rhodomonas Salina Toward Physicochemical Factors Endar Marraskuranto; Tri Joko Raharjo; Rina Sri Kasiamdari; Tri Rini Nuringtyas
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 14, No 1 (2019): May 2019
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v14i1.379

Abstract

Rhodomonas salina produces Cr-phycoerythrin545 as its designated phycoerythrin (PE) with an absorption maximum at 545 nm and a shoulder 564 nm. PE has potential to be applied as colorants, pharmaceutical agents, and fluorescent dye tags. The stability of the PE color is influenced by the physicochemical factors of the solution. This study aimed to analyze the color stability of PECE against chemical (ethanol and pH) and physical (light and temperature) factors. PECE was prepared from freeze-dried biomass of R. salina and was extracted in phosphate buffer solution (pH = 6.0) using a freeze-thaw method in -25 oC (2 hours) and 4 oC (24 hours). The resulting extract was concentrated and dried in a freeze-dryer. Analyses were conducted using UV-visible and fluorescence spectrophotometer. PECE showed color stability against light of white fluorescent lamp exposure up to 8 hours, temperature exposure up to 40 oC, ethanol solution up to concentration of 20 % (v/v), and pH range 3.9-8.42. Results from this study can be useful for extraction, purification, and future application of Cr-PE545.
Callus Induction from Cotyledon of Gyrinops versteegii (Gilg.) Domke Fajri Marisa; Lisna Hidayati; Aries Bagus Sasongko; Tri Rini Nuringtyas
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2629

Abstract

Gyrinops versteegii is an endemic plant in eastern Indonesia that produced agarwood with high quality and economic value. This plant has been threatened by overexploitation which leads on decreasing in the natural population. This research aimed to induce in vitro callus formation to support the sustainable utilization of G. versteegii, which in the end, may support plant productivity. Callus induction was investigated using cotyledon and cultured on MS medium supplemented with several combination of plant growth regulators. The observations were done for the number of explants turned into callus and the duration for the first callus formation. The results showed that combination of 3 mg/L NAA + 0.5 mg/L BAP was recorded as the best combination for callus induction (63.63%). Callus with friable structure and bright color are obtained within nine days of incubation and showed the characteristic of embryonic callus. This result is expected to give a significant opportunity to conserve the natural population of G. versteegii.
Metabolite profiling of agarwood (Gyrinops versteegii (Gilg.) Domke) leaves from difference growth locations using Thin Layer Chromatography Dwi Indriani Manurung; Lisna Hidayati; Nastiti Wijayanti; Tri Rini Nuringtyas
Jurnal Biologi Tropis Vol. 21 No. 2 (2021): Mei - Agustus
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i2.2710

Abstract

Gyrinops versteegii (Gilg) Domke is an agarwood-producing plant originating from Eastern Indonesia. Leaves have antioxidant and anticancer activity due to their secondary metabolites. However, secondary metabolites are strongly influenced by the environment. This study aims to compare the terpenoid, phenolic, and flavonoid profiles of agarwood leaves grown in three locations, namely Bogor, Mataram, and Sambas using Thin Layer Chromatography (TLC), in order to obtain the most potential agarwood G. versteegii for further development as herbal medicine. Agarwood leaf powder was extracted using Soxhlet with 80% methanol as solvent, followed by liquid-liquid fractionation using chloroform and aquadest. The crude extract and the obtained fraction were subjected to metabolite profiling using TLC. Racing data factors from TLC were analyzed using hierarchical cluster analysis (HCA). The results showed that the Mataram sample produced higher yields for both extracts and fractions. TLC detected that the chloroform fraction contained more terpenoids, followed by phenolics and flavonoids, while the aqueous fraction contained the same number of positive bands for terpenoids and phenolics. In terms of origin, the Bogor sample showed six terpenoid positive bands higher than the other samples. A similar pattern was observed in the phenolics, while the flavonoids were the same for all samples. HCA resulted in Mataram being clustered separately from the Bogor and Sambas samples. Agarwood leaves from Mataram cannot be used to replace agarwood leaves from Bogor and Sambas as herbal ingredients.
Immunomodulatory Activity of Agarwood Aquilaria malaccensis Lamk. Leaf Extracts on Staphylococcus aureus-infected Macrophages in vitro Hiqmah Yusi Yana; Lisna Hidayati; Nastiti Wijayanti; Tri Rini Nuringtyas
The Indonesian Biomedical Journal Vol 14, No 2 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i2.1810

Abstract

BACKGROUND: Aquilaria malaccensis has been consumed as herbal medicine, and in vitro study showed that the leaf extract possesses high antioxidant activities. A brief preliminary study indicated that A. malaccensis showed a promising immunomodulatory activity when evaluated using latex beads. This current study aimed to evaluate the immunomodulatory activity of A. malaccensis leaf extract on the macrophage, which was challenged with pathogenic bacteria Staphylococcus aureus.METHODS: Bioactivity was determined by evaluating the phagocytic capacity of macrophages isolated from Mus musculus against S. aureus. First, the cytotoxicity of extracts on macrophages was evaluated using MTT assays, and the IC50 value was used to determine the dose of immunomodulatory assays. The highest toxicity was observed on chloroform extract with an IC50 value of 111.4 µg/mL. Therefore, the treatment was 100 and 50 µg/mL. Two parameters, including the phagocytic activity and phagocytic capacity of macrophages infected with S. aureus, were used to evaluate immunomodulatory activity. The analysis of variance was done at p<0.05 to determine the significant difference among treatments.RESULTS: Chloroform and ethanol extracts at a 50 µg/mL concentration showed the best results with the phagocytic activity of 82.33%±9.61% and 80.33±1.53%. The ethyl acetate showed lower phagocytic activities of 70.67±1.53. All extracts significantly increased phagocytic activity and phagocytic capacity, and the results differed significantly between negative and positive controls. Thin-layer chromatography indicated that the extract contained terpenoid, flavonoid, phenolic, and tannin.CONCLUSION: A. malaccensis leaf extracts showed immunomodulatory activity. Both chloroform and ethanol extracts showed comparable activity, while the ethyl acetate extract was lower. The extracts contained diverse bioactive compounds that may support activating macrophage cells for immunomodulatory activity.KEYWORDS: Aquilaria malaccensis, immunomodulator, phagocytosis, macrophages, Staphylococcus aureus
Immunomodulatory and Antioxidant Activity of Green Grass Jelly Leaf Extract (Cyclea barbata Miers.) In Vitro Mustafid Rasyiid; Rendi Mahadi; Krisnanda Surya Dharma; Lindia Anggraini; Rahma Nurdiyanti; Tri Rini Nuringtyas
Advances in Tropical Biodiversity and Environmental Sciences Vol 2 No 1 (2018): ATBES
Publisher : Institute for Research and Community Services Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.378 KB) | DOI: 10.24843/ATBES.2018.v02.i01.p03

Abstract

Green grass jelly (Cyclea barbata Miers.) is known for its benefit to human health especially in supporting body’s immune system and wellness. This research aimed to determine immunomodulatory and antioxidant activity of green grass jelly leaf extracts in vitro. Old leaves were collected as sample then dried and ground to powder. The extraction was done with sohxletation using three different solvents, chloroform, ethyl acetate, and ethanol. The immunomodulatory activity was evaluated by treating the crude extracts at concentrations of 50, 100, and 500 mg/mL on macrophages of rat in vitro. The treated macrophage was then challenged for their phagocytic activity to latex beads. The antioxidant activity was done using 1,1-diphenil-2-picrilhydrazil (DPPH) with spectrophotometry technique. All treatments were done with three replicates. Detection of the bioactive groups of the extracts was done by Thin Layer Chromatography (TLC). The results showed that ethyl acetate extract has the highest phagocytic activity followed with chloroform extract and ethanol extract, respectively. Optimum concentration was reached at 100 mg/mL of ethyl acetat extract. The ethyl acetate extract was also the extract with the highest antioxidant activity index 7.7 followed by both extracts of chloroform and ethanol with similar index value of 6.25 and 6.3, respectively. The ethyl acetate extract contained phenolics, flavonoids, tannins, and terpenoids.
The Performance of Legume and Non-legume Trees under Dry Karst Areas Hendrati, Rina Laksmi; Baskorowati, Liliana; Mashudi, Mashudi; Nurtjahjaningsih, ILG; Pudjiono, Sugeng; Setiadi, Dedi; Sumardi, Sumardi; Pujiono, Eko; Nuringtyas, Tri Rini; Wibisono, Mochamat Gunawan
Jurnal Manajemen Hutan Tropika Vol. 30 No. 2 (2024)
Publisher : Institut Pertanian Bogor (IPB University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.7226/jtfm.30.2.265

Abstract

Karst areas in Indonesia are arid landscapes with water-use limitations because of dissolved carbonates. Long-term water scarcity stunts plant growth and often kills them. For tropical karst forestry-greening, the three best legume and three best non-legume species from a previous trial comprising 20 species were compared. Since October 2011, seedlings of the top three non-legume, species Aleurites mollucana, Sterculia foetida, and Alstonia scholaris, and three legume species, Acacia auriculiformis, Cassea seamea, and Acacia mangium, have been grown for four months. In January 2012, field trials were established at two dry karst locations, i.e., Pracimantoro, Central Java, and Bunder, Gunung Kidul Yogyakarta. A randomized block design was used to raise 1.764 seedlings at the two sites with 7×7 plots, 3×3 spacing between trees, and three blocks. After 10 months, legumes and non-legumes differed greatly in all growth parameters. These disparities lasted up to 30 months, when trees should have adapted to their new surroundings. After seven years of planting, legume trees raised the soil's organic matter concentration from low to medium, making it more fertile, similar to soil from intensive agricultural regions. Thus, early or mixed legume plantings on tropical karst sites may aid in better re-greening than the establishment of non-legumes.
Optimization of qRT-PCR Annealing Temperature of WRKY45 Gene for Detection of Resistance Genes Against Xanthomonas oryzae pv. oryzae on Black Rice Cempo Ireng Triyaningsih, Triyaningsih; Nuringtyas, Tri Rini; Purwestri, Yekti Asih; Sebastian, Alfino
Jurnal ILMU DASAR Vol 23 No 1 (2022)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v23i1.24181

Abstract

Plant pathogens constrain the development of black rice farming. One of these pathogens is Xanthomonas oryzae pv. oryzae (Xoo), causing a bacterial leaf blight disease. The disease disrupts crop growth and reducing yields. Cempo ireng is a local pigmented rice cultivar from Yogyakarta, Indonesia, which is reported for its high resistance to Xoo. One of the rice resistance mechanisms to Xoo infection is a molecular defense employing plant resistance genes such as WRKY45. Comparing the expression of resistance-related genes of the resistant cultivar to the susceptible ones is needed to elucidate the resistance mechanism of the black rice to Xoo. For this purpose, the expression of WRKY45 gene at the level of mRNA can be performed using qRT-PCR. The success of qPCR analysis is greatly influenced by the accuracy of the annealing primer temperature of the corresponding gene. This study aimed to optimize the primer's annealing temperature for WRKY45 gene. The optimization was done by a temperature gradient PCR. Determination of the optimal annealing temperature was selected based on the profile of the amplification curve, melt curve, melt temperature and the Ct value obtained. The annealing temperature gradient used in this study was ranging from 52°C to 60°C. The results showed that the best annealing temperature for WRKY45 gene primers is 58.3°C based on the amplification curve, melt curve, melt peak and Ct value of 29.21.
Prospecting New Antimicrobial Peptide from Gracilaria spp. Active-peptide Hydrolysate against Pathogenic Bacteria using Proteomic Approach Chandra, Klara Kharisma Bunga; Nuringtyas, Tri Rini; Raharjo, Tri Joko
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 19, No 3 (2024): December 2024
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.942

Abstract

Our study focused on unveiling the tropical red algae Gracilaria spp. Active-peptide materials from the central region of Java Island, Indonesia. We described the protein isolation, hydrolysis, purification, and method to test its potency against pathogen Staphylococcus aureus IFO 12576 and Eschericia coli IFO 3301, followed by molecular docking analysis. TCA/acetone precipitation was used to isolate Gracilaria spp. proteins, and the hydrolysis was  done by trypsin digestion with an effective yield to provide antibacterial activity. The disk diffusion method showed promising inhibition and continued with a confirmation test using microdilution, which implied bacteriostatic inhibition with  a minimum concentration of 40 µg/ml, from one potent fraction. Further characterizations were conducted using a proteomic approach. LC-HRMS was used for peptide sequencing in the potent fraction with prospective peptides identified along with its physical properties. Molecular docking simulations  were used to investigate the degree of interactions using binding affinities score (kcal/mol), with the target of receptor DraE adhesin subunit (2JKJ) of Escherichia coli binds chloramphenicol. We proposed the interactions model of peptides GP1 and GP4 against targeted peptides. Our model GP4.1 and GP4.2 model (VVINADAK) were found to have high binding affinities with the energy score of -10,6 and -10,8 kcal/mol, respectively  by the different sites of binding than chloramphenicol succinate.
Co-Authors Adita Yuniati Puspitasari Afifah, Laelatul ALIM ISNANSETYO Amalia, Alifa Andi Nur Cahyo Andyra, Vania Uly Aries Bagus Sasongko Caroline Dwiseptianti Chandra, Klara Kharisma Bunga Charlie Ester de Fretes Damayanti, Frisca Dedi Setiadi Denis FABRE Dewi, Siska Noviana Diani Mentari Dio N. Wijaya Donny Widianto Dwi Indriani Manurung Dwiseptianti, Caroline Dyah Ismoyowati, Dyah Eka Tarwaca Susila Putra Eko Pujiono Endar Marraskuranto Enik Nurlaili Afifah Erwin Nur Indiarto Fajri Marisa Febri Adi Susanto Febri Adi Susanto Harno Dwi Pranowo Hiqmah Yusi Yana ILG Nurtjahjaningsih, ILG JAKA WIDADA Krisnanda Surya Dharma Krisnanda Surya Dharma L. Hartanto Nugroho Lailaty, Intani Quarta Liliana Baskorowati Lindia Anggraini Lindia Anggraini Lisna Hidayati Marlyn Dian Laksitorini, Marlyn Dian Mashudi Mashudi Megayani Sri Rahayu Mirtani Naima Muhammad Imam Surya Muhammad Nursid Mustafid Rasyiid Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Nastiti, Anisa Diana Novitasari, Laras Nugroho, Hartanto Nurrahmi Dewi Fajarningsih, Nurrahmi Dewi Pascal MONTORO Rahma Nurdiyanti Rahma Nurdiyanti Rahmawati, Rahmawati Rendi Mahadi Rendi Mahadi Respati Tri Swasono Rina Laksmi Hendrati Rina Sri Kasiamdari Riris Istighfari Jenie Riska Wulansari Ronny Martien Rudi Hari Murti Rudi Hari Murti Sebastian, Alfino Setiowati, Retno Diah Sri Wening Sudarsono Sugeng Pudjiono, Sugeng Sumardi . Surta, Muhammad Imam Susanto, Febri Adi Tri Joko Raharjo Tri Joko Raharjo Tri Wibawa Triyaningsih Triyaningsih, Triyaningsih Wibisono, Mochamat Gunawan Wulansari, Riska Yekti Asih Purwestri Yosi Bayu Murti Zulkifli Zulkifli