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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Dasar Indonesian Journal of Cancer Chemoprevention AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Bioeksperimen: Jurnal Penelitian Biologi Pharmacon Majalah Obat Tradisional Journal of Tropical Biodiversity and Biotechnology The Indonesian Biomedical Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Biologi Tropis JSFK (Jurnal Sains Farmasi & Klinis) Menara Perkebunan Advances in Tropical Biodiversity and Environmental Sciences Proceeding Biology Education Conference Berkala Ilmiah Biologi
Tri Rini Nuringtyas
Department Of Biology, Faculty Of Biology, Universitas Gadjah Mada, Jl. Bulaksumur, Sleman, DI Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Veterinary

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Formulation and Characterization Self-Nanoemulsifying Drug Delivery Systems (SNEDDS) Chloroform Extract of Gaharu Leaves (Gyrinops verstegii (Gilg.) Domke Dewi, Siska Noviana; Martien, Ronny; Novitasari, Laras; Nuringtyas, Tri Rini
JSFK (Jurnal Sains Farmasi & Klinis) Vol 11 No 3 (2024): J Sains Farm Klin 11(3), December 2024
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.11.3.179-188.2024

Abstract

Gaharu (Gyrinops versteegii (Gilg.) Domke) is a native Indonesian plant with many potential medicinal compounds. The compounds contained in the chloroform extract of gaharu leaves are mostly nonpolar terpenoids and fatty acids, so their solubility in water is low. Self-nanoemulsifying drugs delivery system (SNEDDS) technology can increase the solubility and bioavailability of drugs or active compounds in cells. This study aims to formulate and characterize SNEDDS from chloroform extract of gaharu leaves. The research began with the extraction of gaharu leaves by soxhletation. Formula optimization was carried out with various oils, surfactants, and cosurfactants. SNEDDS preparations were then characterized by testing particle size, polydispersity index, percent transmittance, and determining globule morphology. The optimal SNEDDS formula contained miglyol® 812N oil and Tween 80 (32%:68%) with particle size 63.25 ± 5.93 nm, polydispersity index 0.272 ± 0.016, and percent transmittance 98.33 ± 0.23%, spherical globule morphology. This study concluded that the optimal formula for SNEDDS chloroform extract of gaharu leaves (Gyrinops versteegii (Gilg.) Domke) had good physical characteristics. Keywords: Gyrinops verstegii (Gilg.) Domke; gaharu; SNEDDS gaharu; miglyol
Cytotoxic Activity of Castanopsis argentea (Blume) A.DC. Extract and Fraction on MCF-7 Breast Cancer Cell Nuringtyas, Tri Rini; Nastiti, Anisa Diana; Surta, Muhammad Imam; Lailaty, Intani Quarta; Damayanti, Frisca
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.9851

Abstract

Marine algae is a biological source that is rich in bioactive activity. Marine algae is an important source of macronutrients, especially proteins and lipids, as well as micronutrients such as vitamins and minerals along with dietary fiber and other minoritarian constituents such as polyphenols. This study aimed to determine the antioxidant of hydrolysate protein of Sargassum sp. as. The work method was started by collecting Sargassum sp. in the intertidal zone of the South Coast of Gunungkidul, Yogyakarta and then brought to the laboratory for extraction. Protein hydrolysate was obtained by protein extraction using phosphate buffer and triRNA kit. Several buffers was used to optimize the extraction, namely PBS buffer, Tris HCl buffer and plant extraction buffer The protein obtained were evaluated in the SDS-PAGE electrophoresis. The isolated protein subjected for the trypsin digestion to obtain the hydrolysate protein then followed by antioxidant assays using DPPH test. Data analysis was carried out using ANOVA statistics for the antioxidant test. Several ratios of trypsin and protein are used to find the optimal ratio to produce protein hydrolysate. From the result it was observed that the best buffer solvent to use was Tris HCl buffer for the isolation of Sargassum sp. This can affect the protein profile of the Sargassum sp protein hydrolysate. by producing higher protein precipitates than other buffer types.
Antibacterial Activity of Leaf and Twig Extracts of Castanopsis argentea (Blume) A.DC. Against Escherichia coli and Staphylococcus aureus Amalia, Alifa; Lailaty, Intani Quarta; Surya, Muhammad Imam; Nuringtyas, Tri Rini; Damayanti, Frisca
Berkala Ilmiah Biologi Vol 16 No 1 (2025)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v16i1.19731

Abstract

Castanopsis argentea is a potential souce of natural antibiotics that has not been studied much. The purpose of this study was to analyze the antibacterial activity and study the content of secondary metabolites from leaves and twigs extract of Castanopsis argentea against Escherichia coli and Staphylococcus aureus bacteria. This study used the paper disk method to see antibacterial activity. Antibacterial activiy was indicated by the formation of a clear zone around the dic paper. Samples were made into three treatments, namely the concentration of 20 mg/ml; 40 mg/ml; and 80 mg/ml. the solvent used was selected based on the level of polarity namely ethyl acetate, 70% ethanol, and aquades. The results of the antibacterial activity test showed that the extract with the most potential to inhibit bacterial growth was ethyl acetate twig extract at a concentration of 80 mg/ml with an average diameter of 6mm for Escherichia coli bacteria and 8.67 mm for Staphylococcus aureus bacteria. The compounds identified in the ethyl acetate twig extract are terpenoids, phenolics, and flavonoids. The results of profiling using UV-Vis spectrophotometry showed that the maximum absorption of terpenoids, flavonoids, and phenolics compounds was found in the wavelength range of 300-600 nm. The secondary metabolite compounds contained in the leaf and twig extracts of Castanopsi argentea make this species a potential natural antibacterial.
SPAD-502 and atLEAF CHL PLUS values provide good estimation of the chlorophyll content for Hevea brasiliensis Müll. Arg. Leaves Andi Nur CAHYO; Rudi Hari MURTI; Eka Tarwaca Susila PUTRA; Tri Rini NURINGTYAS; Denis FABRE; Pascal MONTORO
Menara Perkebunan Vol. 88 No. 1 (2020): 88 (1), 2020
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v88i1.369

Abstract

Measurement of chlorophyll content using destructive methods is not efficient due to a large number of samples, cost, and time needed. Estimationof chlorophyll content by nondestructive methods using handheld chlorophyll meter may be considered to improve efficiency. This research aimed to determine the formula to convert SPAD-502 and atLEAF CHL PLUS values (relative indicator of chlorophyll content) to estimated (absolute) rubber leaves chlorophyll content. Twenty leaves of rubber plant were measured using SPAD-502 and atLEAF CHL PLUS at the same time to determine SPAD-502 and atLEAF CHL PLUS values. The measured leaves were then collected to determine the chlorophyll content using a standard laboratory procedure. Regression and correlation analyses (among 3 methods) were conducted using SAS v.9 software. The results showed that between SPAD-502 and atLEAF CHL PLUS values were closely correlated, hence both of the devices can substitute each other to estimate rubber leaf chlorophyll content. In addition, the relationship between atLEAF CHL PLUS and SPAD-502 values with actual chlorophyll content of rubber clone SP 217, PB 260, GT1, and all clones (general) were significant with high coefficient of determination (R2) as well as low Root Mean Square Error (RMSE) and Coefficient of Variation (CV). Therefore, by using formula determined in this study, both atLEAF CHL PLUS and SPAD-502 can be suggested for accurate, fast, and non-destructive estimation of chlorophyll content of rubber plant leaf.
Identification of metabolites for biomarker of nitrogen and potassium use efficiency in oil palm Setiowati, Retno Diah; Sri Wening; Nuringtyas, Tri Rini; Rahayu, Megayani Sri; Sudarsono, Sudarsono
Menara Perkebunan Vol. 93 No. 1 (2025): 93(1), 2025
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v93i1.631

Abstract

Nutrient-use efficiency in oil palm is important for economic and environmental reasons. This research aimed to identify biomarkers to discriminate between tolerant and susceptible oil palms to potassium (K) and nitrogen (N) deficiency. A screening of oil palm materials for N or K use efficiency was conducted using an omission trial experiment, where only targeted nutrient was applied as treatment, while all other nutrients were applied as recommended. The treatment was performed in the main nursery for ten months to identify progenies with contrasting traits. Metabolite analysis was performed to identify specific metabolites as biomarkers for N-efficient and K-efficient palms. Samples taken from the roots of the contrasting progenies were treated with liquid nitrogen prior to grinding into a powder for liquid chromatography-high resolution mass spectrometry (LC-HRMS) analysis. The LC-HRMS analysis showed 277 metabolites from K and N treatments after data trimming, which were then analysed in MetaboAnalyst 6.0 for biomarker identification. The results showed that some metabolites were statistically significant. Metabolites identified in more than one analysis have a higher likelihood of being considered as biomarkers. In this experiment, we compared PLS-DA, sPLS-DA, and Random Forest. However, some identified metabolites were not to occur naturally in the treatment palms. Some amino acids and antioxidants were promising biomarkers to differentiate the N-deficiency-tolerant and K-deficiency-tolerant palms. Thus, the biomarkers facilitate the breeding scheme to create a nutrient-efficient palm planting material.
Design and Stability Evaluation of Active Peptides from Indonesian Echinozoa as Acetylcholinesterase (AChE) Inhibitors for Alzheimer’s Therapy Afifah, Laelatul; Andyra, Vania Uly; Laksitorini, Marlyn Dian; Nuringtyas, Tri Rini
The Indonesian Biomedical Journal Vol 17, No 5 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i5.3837

Abstract

BACKGROUND: Alzheimer’s disease is characterized by cognitive decline resulting from decreased acetylcholine (ACh) levels due to excessive acetylcholinesterase (AChE) activity. Current therapies, such as galantamine, have several side effects. Bioactive peptides derived from marine Echinozoa (sea urchins and sea cucumbers) have emerged as promising therapeutic agents owing to their structural diversity and diverse bioactivities. Previous studies identified peptides from sea cucumbers and sea urchins collected along the southern coast of Gunung Kidul, Yogyakarta (VLCAGDLR, SWIGLK, MNGKKITVRPR, and KTKDLK), which exhibit acetylcholinesterase (AChE) inhibitory activity. However, the therapeutic use of these peptides is challenged by blood–brain barrier (BBB) penetration and stability issues. Therefore, this study was conducted to identify candidate peptides through in silico analysis and to evaluate their stability in phosphate-buffered saline (PBS) as potential AChE inhibitors.METHODS: Molecular docking was conducted to evaluate peptide binding affinity to the active site. The best candidate peptides were synthesized and tested in vitro for AChE inhibition using a colorimetric method. Stability was assessed in PBS by monitoring aggregation through turbidity and Congo Red assays.RESULTS: The sea cucumber peptide SWIGLK showed strong binding affinity (–10.2 kcal/mol) and 12.11% inhibition at 0.19 mM, while the sea urchin peptide KTKDLK exhibited –8.2 kcal/mol and 11.50% inhibition at 0.19 mM. Both peptides remained stable in PBS without aggregation for up to 48 h.CONCLUSION: SWIGLK and KTKDLK demonstrate the most significant AChE inhibitory activity and maintained structural stability, hence supporting their potential as peptide-based candidates for Alzheimer’s therapy.KEYWORDS: Alzheimer, AChE inhibitor, holothuroidea, echinoidea, bioactive peptide, peptide stability
Antioxidant and Anticancer Activity of Dillenia serrata Thunb Ethanol Extract Against MCF-7 Breast Cancer Cell Line Rahmawati, Rahmawati; Zulkifli, Zulkifli; Nuringtyas, Tri Rini; Jenie, Riris Istighfari; Nugroho, Laurentius Hartanto
Indonesian Journal of Cancer Chemoprevention Vol 13, No 3 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss3pp175-183

Abstract

Women’s breast cancer incidence rate in Indonesia ranks number one with 12 per 100,000 cases, with luminal A as the dominant subtype. Currently, chemotherapeutic agents have limitations that lead to inefficiencies in therapy, therefore it is necessary to develop more effective and efficient chemopreventive agents. Plant secondary metabolites can provide pharmacological effects that can be used as chemoprevention agents. Secondary metabolites of D. serrata may have pharmacological effects as antioxidants and cytotoxic. This study aims to determine the antioxidant properties and cytotoxic activity of D. serrata ethanolic extract on the MCF-7 breast cancer cell line. The leaves of D. serrata were macerated, while the bark and root samples were refluxed with 96% ethanol as solvent. All extracts were evaporated with a rotary evaporator. Qualitative evaluation of the phytochemical content of leaf ethanolic extract, bark ethanolic extract, and root ethanolic extract was done using the standard tube test method. The antioxidant assay was carried out using the DPPH. The cytotoxic activity was determined in vitro using an MTT assay against the MCF-7 cell line with a series of concentrations from 12.5–400 μg/mL. Doxorubicin was the positive control treated at a 3.125–100 μg/mL concentration. The antioxidant activity showed that leaf extract had the highest antioxidant activity, followed by root and bark extract, with IC50 values of 95.66, 270.5, and 335.96 ppm, respectively. Leaf ethanolic extract and root ethanolic extract’s cytotoxic ability is considered moderate cytotoxic with IC50 values of 493.17 and 229.82 μg/mL, respectively. Amongst the ethanolic extract from the leaf, bark, and root of D. serrata, the leaf ethanolic extract has the best anti-oxidant activity and the bark ethanolic extract was the most cytotoxic one against MCF-7 cells.Keywords: Antioxidant, Cytotoxic, Dillenia serrata, MCF-7.
Comparative Study of Pre-Extraction Treatments and Buffer Modifications for Macroalgal Lectin Extraction Fajarningsih, Nurrahmi Dewi; Nursid, Muhammad; Nugroho, Hartanto; Nuringtyas, Tri Rini; Isnansetyo, Alim
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 18, No 3 (2023): December 2023
Publisher : :Agency for Marine and Fisheries Research and Human Resources, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.852

Abstract

Getting an efficient extraction approach is a crucial step in bioactive protein research, particularly lectin. This research aimed to examine the efficiency of cryogenic-grinding (CG) and freeze-dried-grinding (FG) pre-extraction treatments, and also the incorporation of phenylmethylsulphonyl fluoride (PMSF), Tween 80, polyvinylpolypyrrolidone (PVPP), 70% Ethanol (EtOH), or combination of the chemicals in the 20 mM phosphate buffered saline pH 7 (PBS) for extracting lectin from Ulva lactuca, Sargassum polycystum, and Hydropuntia edulis. The lectin content of the extracts was determined using the hemagglutination activity (HA) assay. The phenolic content was measured to determine its impact on the lectins’ HA. Lectin extraction efficiency was determined by analyzing the extracts’ minimum agglutination concentration (MAC) and total hemagglutination activity (THA). CG pre-extraction treatment produced slightly higher THA than FG, making it slightly more efficient. The EtOH treatment efficiently extracted lectin from U. lactuca and H. edulis by substantially reducing the polyphenol (PPs) content, lowering the MAC, and increasing the THA. The EtOH treatment significantly decreased the PPs and HA of the S. polycystum, suggesting that the HA is most likely produced by the PPs rather than the lectin content. Tween 80 raised the THA of U. lactuca by 17-fold with native rabbit erythrocyte compared to the control but did not affect the THA of H. edulis and S. polycystum. Several different effects of chemicals incorporated in the extraction buffers suggested that the optimum macroalgal lectin extraction strategy is species-dependent.
Optimasi Metode 1H-NMR Profiling pada Rimpang Kunyit (Curcuma domestica) Dwiseptianti, Caroline; Susanto, Febri Adi; Purwestri, Yekti Asih; Nuringtyas, Tri Rini
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9238

Abstract

The use of medicinal plants is increasing due to the lack of side effects caused and the number of bioactive compounds that cannot be represented by synthetic chemical synthesis compounds. However, the management and use of natural medicines for the main handling of diseases are often hampered by the quality of the ingredients which are low and unstable. The standardized quality control system of OAI (Indonesian Natural Medicine) is the main key to improve clinical assurance and safety of the use of herbal medicines in Indonesia. One of the medicinal plants known to the public is Curcuma longa L. (turmeric). The main active components contained in turmeric are curcumin, demetoksikurkumin, bis-demetoksikurkumin, and ar-turmeron. Information about the quality of turmeric is needed in its use as a raw material for drugs so we need an analytical technique that is able to identify the diversity of metabolite profiles of active compounds. In this research, an optimization method is used to improve efficiency in the extraction of turmeric rhizome metabolites so that the best solvent concentration is known for the analysis of fingerprinting secondary metabolites with 1H-NMR 500 MHz spectroscopy in turmeric rhizomes. The results were analyzed with MNOVA software and chemical shift obtained compared with the reference. From the results obtained a concentration of methanol-d4 (CD3OD) 100% able to extract curcumin better than other solvents. The solvent is able to extract saccharide (sugar) compounds in the form of sucrose, amino acids and fatty acids in the form of methionine, glutamine, acetate, and glycero phospho choline.
Co-Authors Adita Yuniati Puspitasari Afifah, Laelatul ALIM ISNANSETYO Amalia, Alifa Andi Nur Cahyo Andyra, Vania Uly Aries Bagus Sasongko Caroline Dwiseptianti Chandra, Klara Kharisma Bunga Charlie Ester de Fretes Damayanti, Frisca Dedi Setiadi Denis FABRE Dewi, Siska Noviana Diani Mentari Dio N. Wijaya Donny Widianto Dwi Indriani Manurung Dwiseptianti, Caroline Dyah Ismoyowati, Dyah Eka Tarwaca Susila Putra Eko Pujiono Endar Marraskuranto Enik Nurlaili Afifah Erwin Nur Indiarto Fajri Marisa Febri Adi Susanto Febri Adi Susanto Harno Dwi Pranowo Hiqmah Yusi Yana ILG Nurtjahjaningsih, ILG JAKA WIDADA Krisnanda Surya Dharma Krisnanda Surya Dharma L. Hartanto Nugroho Lailaty, Intani Quarta Liliana Baskorowati Lindia Anggraini Lindia Anggraini Lisna Hidayati Marlyn Dian Laksitorini, Marlyn Dian Mashudi Mashudi Megayani Sri Rahayu Mirtani Naima Muhammad Imam Surya Muhammad Nursid Mustafid Rasyiid Naima, Mirtani Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Nastiti, Anisa Diana Novitasari, Laras Nugroho, Hartanto Nurrahmi Dewi Fajarningsih, Nurrahmi Dewi Pascal MONTORO Rahma Nurdiyanti Rahma Nurdiyanti Rahmawati, Rahmawati Rendi Mahadi Rendi Mahadi Respati Tri Swasono Rina Laksmi Hendrati Rina Sri Kasiamdari Riris Istighfari Jenie Riska Wulansari Ronny Martien Rudi Hari Murti Rudi Hari Murti Sebastian, Alfino Setiowati, Retno Diah Sri Wening Sudarsono Sugeng Pudjiono, Sugeng Sumardi . Surta, Muhammad Imam Susanto, Febri Adi Tri Joko Raharjo Tri Joko Raharjo Tri Wibawa Triyaningsih Triyaningsih, Triyaningsih Wibisono, Mochamat Gunawan Wulansari, Riska Yekti Asih Purwestri Yosi Bayu Murti Zulkifli Zulkifli