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All Journal Jurnal Penelitian Sains Jurnal Ilmiah Dikdaya Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Komposit : Jurnal Ilmu-Ilmu Teknik Sipil Jurnal Pendidikan dan Teknologi Indonesia JURNAL PENDIDIKAN INDONESIA: Teori, Penelitian, dan Inovasi Journal of Governance and Accountability Studies Indonesian Research Journal on Education TOFEDU: The Future of Education Journal Jurnal Ilmiah Pangan Halal HIKMAH: Jurnal Ilmu Dakwah dan Komunikasi Islam DIDAKTIS: Jurnal Pendidikan Bahasa dan Sastra Indonesia Studi Multidisipliner: Jurnal Kajian Keislaman Intelektual: Jurnal Pendidikan dan Studi Keislaman
Afryansyah, Afryansyah
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Simulasi In Silico Integrasi EGF ke Lactobacillus rhamnosus GG melalui CRISPR-Cas9 Sebagai Probiotik Terapeutik Ulkus Kronis: In Silico Simulation of EGF Integration Into Lactobacillus rhamnosus GG Via CRISPR-Cas9 For Therapeutic Probiotics For Chronic Ulcers Rizakiya, Arini; Mutiasari, Alifah Rahma; Fiamanella, Medina; Mutimmah, Mutimmah; Afryansyah, Afryansyah; Jumiono, Aji; Puspasari, Erna
Jurnal Ilmiah Pangan Halal Vol. 8 No. 1 (2026): Jurnal Ilmiah Pangan Halal
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jiph.v8i1.23840

Abstract

Chronic wounds are a health problem characterized by failure to heal within four weeks and the potential for physical and psychological complications. One important factor in wound healing is the availability of Epidermal Growth Factors (EGF), which is often disrupted in chronic wounds. This study aims to investigate in silico the mechanism of CRISPR-Cas9-based genetic engineering of Lactobacillus rhamnosus GG bacteria as a therapeutic probiotic that produces EGF for the healing of chronic ulcer. The research method used a bioinformatics approach that included genetic homology analysis, guide RNA design, recombinant plasmid construction, mRNA secondary structure prediction, protein physicochemical property analysis, and EGF protein tertiary structure modeling. The result showed that the EGF gene has no significant similarity with the Lactobacillus rhamnosus GG genome, the designed gRNA had good specificity and cutting efficiency, and the gene construction was potentially stable and expressed. The stimulated EGF protein showed good three-dimensional structure quality with exposed active residues. Overall, this study shows that the in silico approach has the potential to become the basis for the development of CRISPR-Cas9-based engineered probiotics as an alternative therapy for chronic wound healing.
SINTESIS SILIKA XEROGEL DARI ABU LIMBAH SEKAM PADI (Oryza sativa .L) DENGAN METODE SOL-GEL Afryansyah, Afryansyah; Elmiya, Chalesa; Ghaisani, Jihan
Jurnal Penelitian Sains Vol 28, No 1 (2026)
Publisher : Faculty of Mathtmatics and Natural Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56064/jps.v28i1.1281

Abstract

Rice as the staple food for 90% of Indonesia's population, produces abundant unprocessed rice husk waste from processing, posing serious environmental threats. This research aims to convert the silica (SiO₂) content—ranging from 87%-97%—in rice husks into silica xerogel with significant economic potential through the sol-gel method. The synthesis involved several stages: preparation and calcination of rice husks, silica extraction using HCl and NaOH solutions to obtain sodium silicate, followed by neutralization to form silica hydrogel, and finally conversion into silica xerogel. The final product was analyzed through organoleptic tests, moisture content, and adsorption ability, where the organoleptic test results showed the physical form of weak solids (gel) on silica hydrogel, sandy grains on silica xerogel, as well as colorless and odorless on both. The moisture content of silica xerogel was 20%, compared to 10% in commercial silica gel. Meanwhile, adsorption testing revealed the percentage of adsorption ability of the synthesized silica was two times higher, which is 32.35% compared to the commercial one of 14.7%. It proved to be an effective short-term moisture adsorbent with significant absorption capability. This study highlights the potential of rice husk-derived silica xerogel as an effective, economical, and environmentally friendly alternative humidity adsorbent.
Co-Authors Abdullah Idi Aji Jumiono Andre Andre, Andre Bahrudin, Andre Bustomi, Muhammad Effendi, Darwin Elmiya, Chalesa Erna Puspasari Fachriyadi, Ahmad Naufal Fadhila Muhammad Libasut Taqwa Feril Hariati Fiamanella, Medina Fikri, Aiman Fitriani, Yessi Ghaisani, Jihan Giyoto Hawa, Komariah Hepriyadi, Hepriyadi Hidayatullah , Hidayatullah hidayatullah, Hidayatullah hidayatullah Ismail Ismail Ismail Sukardi, Ismail Ismail, Farji Karoma, Karoma Karomah Karomah Liyantifa, Barliyana Mardiah Astuti Marlisah, Marlisah Muchtar, Aflatun Muhammad Ikbal Mutiasari, Alifah Rahma Mutimmah, Mutimmah Nurbuana, Nurbuana Ramadhona, Rossa Rizakiya, Arini Romli SA, Romli SA Sirozi, Muhammad Sukainah, Sukainah Zahro, I.M. Fatimah Zahro, I.M.Fatimah Zuhdiyah Zuhdiyah, Zuhdiyah