Program Learning Outcomes (PLO) assessment is an important part of Outcome Based Education curriculum. This present study aims to directly measure the PLOs’ achievement in the bachelor program of Nutrition, Universitas Negeri Surabaya. The PLOs of the program study consist of three domain aspects namely knowledge, skill, and attitude. The direct measurement was derived from students’ performances in a total of 16 courses taught in even semesters (semesters 2, 4, 6, and, 8) of the program study. The students’ performances were assessed based on their performances in quizzes, classroom participation, mid-semester test, and final semester test by using a rubric of measurement. From the total of 9 PLOs, only 7 PLOs are assessed because in the even semesters, there was no course containing PLO 4 and 9 for their objective. Overall, the PLO mean score was ranging from 73.8 to 82.2. The domain of knowledge has the lowest score compared to the domain of skill and the domain of attitude although the gap difference is very small.