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All Journal Jurnal Penelitian Hasil Hutan Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Riset Industri Hasil Hutan Jurnal Hutan dan Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Agrotek UMMat SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Biosaintifika: Journal of Biology & Biology Education EMBRIO : Jurnal Kebidanan Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat IJEEM - Indonesian Journal of Environmental Education and Management Jurnal Kridatama Sains dan Teknologi ULIN: Jurnal Hutan Tropis Jurnal Abdi Insani Jurnal Penelitian Hasil Hutan Journal of Forest Science Avicennia Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat Jurnal Silva Samalas: Journal of Forestry and Plant Science Jurnal Ilmiah Sangkareang Mataram Empiricism Journal Journal of Agritechnology and Food Processing Rona Teknik Pertanian Prosiding Seminar Nasional Unimus Biosaintifika: Journal of Biology & Biology Education International Journal of Ethnoscience and Technology in Education Sasambo: Jurnal Abdimas (Journal of Community Service)
Wangiyana, I Gde Adi Suryawan
Program Studi Kehutanan Universitas Pendidikan Mandalika
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MEDICINAL USAGE OF AGARWOOD RESIN IN FORM OF ESSENTIAL OIL: A REVIEW I Gde Adi Suryawan Wangiyana
Jurnal Silva Samalas Vol 2, No 2 (2019): Desember 2019
Publisher : Universitas Pendidikan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jss.v2i2.3674

Abstract

Agarwood is a high value of non-timber commodities from Indonesia. Agarwood trade on Indonesia mostly being sold in form of raw material. This raw material is agarwood resin or also well known as ”gubal’. Processing gubal into essential oil will improve the quality and price of this commodity. Furthermore, agarwood essential oil has been reported being used as traditional medication in some countries. This medicinal activity of agarwood essential oil has been confirmed by clinical research and resulted that this material has antioxidant, antibacterial, antifungal and anticancer activity. This material also has neural activity on the nervous system and becomes a potential antidepressant, relaxative, sedative and analgesic agent. The promising of the medicinal use of agarwood essential oil suggested that processing agarwood resin into essential oil should be the number one priority on agarwood trade.
DNA BARCODING LIBRARY DATABASE OF AQUILARIA MEMBER AND GYRINOPS MEMBER I Gde Adi Suryawan Wangiyana
Jurnal Silva Samalas Vol 3, No 2 (2020): Desember 2020
Publisher : Universitas Pendidikan Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/jss.v3i2.3693

Abstract

Aquilaria and Gyrinops are the primary agarwood producer on international trade. For authentication and standardization purposes, it is essential to carry DNA barcoding studies of these genera. DNA barcoding studies on plants need a database of several regions on the plant genome that could act as a barcoding marker. These DNA barcoding markers could be divided into Chloroplasts barcoding and Nuclear barcoding. Several markers have been used for DNA barcoding study of agarwood producer species, including trnL-trnF, matK, rbcL, rpoC1, ycf1 (Chloroplast barcoding), and ITS (Nuclear barcoding). This review breakdown the availability of those DNA barcoding markers on the online genebank database for Aquilaria and Gyrinops. Aquilaria genus has 12 species members, while Gyrinops genus has six species members. The sequence of region trnL-trnF is the only barcoding marker covering all 12 species members of Aquilaria and six species members of Gyrinops. Both ITS and matK have covered nine species among 12 total species members of Aquilaria. The rbcL, rpoC1, and ycf1, respectively, have covered eight, five, and four species members of Aquilaria. Most of the barcoding markers have covered three species members of Gyrinops except for ITS (5 species) and rpoC1 (1 species). However, Gyrinops members have no ycf1 sequence on genebank database. Based on sequence availability on the genebank database, it could be concluded that the trnL-trnF region is the most promising DNA barcoding marker for the Aquilaria and Gyrinops members especially for the phylogenetic analysis purpose.
Pengolahan Sampah Buah dan Sayur dari Pasar Induk Mandalika Kota Mataram Nusa Tenggara Barat Sebagai Media Tumbuh Fusarium sp Inokulan Gaharu I Gde Adi Suryawan Wangiyana; Arbi Sanjaya; Lutfi Anggadhania
IJEEM - Indonesian Journal of Environmental Education and Management Vol 5 No 2 (2020): IJEEM: Indonesian Journal of Environmental Education and Management, Volume 5 Nom
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (366.908 KB) | DOI: 10.21009/IJEEM.052.06

Abstract

The purpose of this research is to process fruit waste and vegetable waste from Mandalika Market at Mataram City as growth medium for Fusarium Sp agarwood inoculant. Collected fruit waste and vegetable waste were processed by grinding and filtrating into filtrate part and residue part. Filtrate part and residue part were formulated as combination growth media including: Fruit waste filtrate, vegetable waste filtrate, mix fruit-vegetable waste filtrate, fruit waste residue, vegetable waste residue and mix fruit-vegetable residue. Fusarium Sp. were sub-cultured into PDA before cultured on combination growth media. Colony diameter of Fusarium isolates were measured every day for seven days incubation period. Fusarium Sp. growth on media from fruit waste was better than its growth on media from vegetable waste. Fusarium Sp. has higher colony diameter on residue growth media compare to its colony diameter on filtrate growth media. On the other hand, filtrate growth media could support Fusarium Sp. to produce several pigmentations. This pigmentation could be an important key character for further taxonomical study. It could be concluded that fruit waste and vegetable waste from residue part could support maximum diameter colony of Fusarium Sp. while filtrate part of this waste could support Fusarium Sp. to produce pigmentation for taxonomical purpose.
DEVELOPMENT OF INDUCTION TECHNOLOGY ON AGARWOOD CULTIVATION - A REVIEW I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 6 No. `1 (2020): Maret 2020
Publisher : SANGKAREANG

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Abstract

Agarwood is one of high valuable non-timber forest commodity produce by family thymeleaceae, especially genus Aquilaria and Gyrinops. Agarwood commodity on the market only could be sold from cultivated species. Thus, agarwood cultivation method should be fully understood by agarwood farmers and agarwood researchers. Induction technology is one of the most important things on agarwood cultivation method. Induction procedure could induce resin production on agarwood cultivated species. This resin has an aromatic fragrance which well known as “gubal” on the market. It is imposible for cultivated agarwood species to produce gubal without induction process. Induction tehnology has been developed form old traditional method into new modern method. Each method has seraval advantages and disadvantages. Old traditional method induced agarwood physically using traditional equipment such as: machete, chopper and nail. It is simple method but only produce resin in very small area. Modern method induces agarwood using chemical liquid or microorganism culture that could spread all over agarwood stem. This method could induce resin in a very wide area compare to traditional method. Resin productivity using modern method is higher than those traditional methods. Thus this modern method induction technology could be best solution for agarwood farmers to increase their agarwood resin.
SIMILARITY ANALYSIS OF GENERA AQULARIA AND GYIRINOPS BASED ON VEGETATIVE STRUCTURE FEATURE USING DIFFERENT CLUSTERING METHOD I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 5 No. 1 (2019): Maret 2019
Publisher : SANGKAREANG

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Abstract

The purpose of this research is to analysis similarity of Aquilaria group and Gyrinops group based on several feature of vegetative organ. Member of Aquilaria Group and Gyrinops group were selecting as OTU based on their validation nomenclature from international taxonomy database. Dendrograms were constructed using MVSP version 3.1A. UPGMA Algorithm were used for clustering method. Two similarity index, Simple Matching Coefficient and Jaccard Coefficient, were used for distance measurement on dendrogram. Cophenetic correlation analysis between similarity matrix were conducted by CoStat Cohort 6. Similarity matrix based on simple matching coefficient were different from similarity matrix based on Jaccard Coefficient on the aspect of OTU pair. Simple Matching coefficient also have higher similarity index then Jaccard coefficient. Moreover, Dendrogram topology based on simple matching coefficient were different from dendrogram topology based on Jaccard coefficient on aspect of sister taxa pairing complex. Cophenetic correlation analysis confirm that distortion data between all similarity matrix were not significant. That means, dendrogram based on these clustering methods were accurate and reliable. It could be concluded that Aquilaria group and Gyrinops group has high similarity index which make them randomly clustering in the same cluster based on its vegetative character feature.
CHARACTERISTIC OF AGARWOOD TEA FROM GYRINOPS VERSTEEGII FRESH AND DRY LEAVES I Gde Adi Suryawan Wangiyana; Sami'un
JURNAL SANGKAREANG MATARAM Vol. 4 No. 2 (2018): Juni 2018
Publisher : SANGKAREANG

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Abstract

Gyrinops versteeegii in Lombok Island mainly be focus on cultivating of agarwood gubal commodity which need up to 14 years for production. Use the leaf to make a tea is one of potential alternative commodity form G. versteegii which give guarantee for agarwood farmers to have a continuous product from their plantation. The purpose of this research is to study different characteristic of tea from fresh and dry agarwood leaves. Leaves sample were taken from the branch near the shoot of G. versteegii tree. After being washed multiple times, 20 gr of fresh leaves were boiled in1 L of 100oC water for 5 minutes. Ten gram of Dry leaves after losing 60% of its water content was brewed in 1 L of 100oC water for 5 minutes. Both fresh leaf tea and dry leaf tea were added with 7,5% w/v sucrose. Fresh leaf tea and Dry leaf tea have a different color, brightness, and turbidity. These 2 types of tea also different in odor and taste. Dry leaf tea has a strong odor and bitter taste while fresh leaf tea has a light odor and weak taste. Slighly bitter taste from dry leaf tea possibly caused by tannin. It could be concluded that agarwood tea from fresh leaf and dry leaf has a different appearance, odor, and taste. Study about phytochemistry of agarwood tea and the hedonic test is needed for better understanding about agarwood tea.
PHYLOGENETIC ANALYSIS OF AQUILARIA AND GYRINOPS MEMBER BASED ON TRNL-TRNF GENE SEQUENCE OF CHLOROPLAST I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 2 No. 4 (2016): Desember 2016
Publisher : SANGKAREANG

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Abstract

Aquilaria and Gyrinops are main agarwood producers that only could be distinguished based on their flower structure. Molecular characteristic analysis is needed to support morphological character for breeding and conservation purpose. Several molecular analyses were conducted based on similarity characteristic of Aquilaria and Gyrinops. However phylogenetic analysis of these genera was so few to support the polyphasic classification. The purpose of this study is to do phylogenetic analysis based on trnL-trnF sequence. Thirteen sequences of Aquilaria and six sequences of Gyrinops were downloaded from NCBI. ClustalX 2.1 program were used for multiple alignment of sequences. Three phylogenetic trees were reconstructed with MEGA 5.1 by different method including: Neighbor Joining, Minimum and Maximum Liklihood. Phydit program were used to construct similarity matrix between sequences. Based on phylogenetic analysis, all Aquilaria members were clustered in the same clade. All sister taxa also composed with Aquilaria member except for A.hirta. On the other hand, G.salicifora, G.Podocarpa and G.Ladermanii are the only Gyrinops members that form same clade. Other Gyrniops members were spread to different clade. It could be concluded that trnL-trnF sequence is a useful phylogenetic marker for Aquilaria although have several limited for Gyrinops. Moreover, based on phylogenetic analysis and similarity analysis it could be suggested that Aquilaria and Gyrinops sould not be separated into different genera.
PEMANFAATAN MEDIUM ALTERNATIF UNTUK PERTUMBUHAN ISOLAT FUSARIUM SP. PENGINDUKSI PEMBENTUKAN GAHARU PADA GYRINOPS VERSTEEGII (GILG) DOMKE I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 1 No. 3 (2015): Desember 2015
Publisher : SANGKAREANG

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Abstract

Gaharu terbentuk di dalam batang Gyrinops versteegii melalui bio-induksi oleh kapang Fusarium sp. Keberhasilan Fusarium menginduksi pelepasan resin sebagai bahan dasar gaharu sangat tergantung pada berbagai faktor. Medium pertumbuhan jamur menentukan kualitas gaharu yang diinduksi pada G. versteegii. Penelitian ini bertujuan untuk melakukan seleksi isolat Fusarium yang mampu menginduksi pembentukan gaharu berdasarkan kemampuan tumbuh dan produksi konidia pada berbagai medium alternatif. Medium alternatif yang digunakan diantaranya: medium serbuk kayu, medium sebuk kayu G. versteegii, medium tauge serta medium kentang (PDA) yang digunakan sebagai kontrol. Sementara itu, isolat Fusarium yang digunakan diantaranya: LU, ALS, LT, LB dan BIMA. Berdasarkan data pertambahan diameter koloni, pertumbuhan tiap isolat pada medium tauge lebih baik dibandingkan PDA. Produksi konidia tiap isolat pada medium tauge juga lebih tinggi dibandingkan medium kontrol PDA. Rerata diameter koloni dan produksi spora tiap isolat pada medium kayu pre-treatment akuades paling mendekati PDA (kontrol). Isolat BIMA merupakan isolat yang memiliki pertumbuhan terbaik dan produksi konidia terbesar dibandingkan isolat lainnya. Data diameter koloni dan produksi konidia tiap medium berbeda secara signifikan berdasarkan ANOVA dan uji lanjut BNJ. oleh karena itu dapat disimpulkan bahwa media tauge dan kayu pre-treatment akuades merupakan medium alternatif terbaik dan sangat potensial digunakan sebagai media inokulan Fusarium penginduksi gaharu. Sementara itu, isolat BIMA merupakan isolat dengan pertumbuhan terbaik yang potensial untuk dikembangkan lebih lanjut sebagai agensia bio-induksi gaharu.
MOLECULAR PHYLOGENETIC ANALYZE OF FUSARIUM FROM AGARWOOD AND OTHERS FUSARIUM WITH DIFFERENT TYPE OF NUTRITION BASED ON GEN ITS 1 I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 2 No. 1 (2016): Maret 2016
Publisher : SANGKAREANG

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Abstract

Fusarium is a common mold used as inoculants for inducing resin formation in agarwood tree. Molecular phylogenetic analyze is important to be done to determine the correlation between type of nutrition of Fusarium and its ability to associated with Agarwood. ITS 1 gene sequences were downloaded from NCBI gene bank was used as sequences to reconstruct phylogenetic tree. ClustalX 2.1 program were used for multiple alignment of sequences. Two phylogenetic tree, Neighbor Joining and Maximum Likelihood, were reconstructed with MEGA 5.1 program. One phylogenetic tree were reconstructed with mrbayes program using Markov Chain Monte Carlo method. Phydit program were used to construct similarity matrix between sequences.Different type of nutrition on Fusarium including: endophytic, saprophytic and phytopathogenic were not a monophyletic based on phylogenetic tree analyze. That type of nutrition was formed by adaptation process. They do not share that characteristic withtheir common ancestor. Fusarium from agarwood has the same clade with phytopathogenic Fusarium in all phylogenetic trees. They also have the highest ITS 1 gene sequence similarity with phytopathogenic Fusarium. Therefore, it could be concluded that Fusarium from agarwood are phytopathogenic group and they have pathogen-host association with agarwood.
INTERACTION OF FUSARIUM SP. WITH GYRINOPS VERSTEEGII SEEDLING BY MORPHOLOGICAL, ANATOMICAL, AND CHEMICAL OBSERVATION I Gde Adi Suryawan Wangiyana
JURNAL SANGKAREANG MATARAM Vol. 3 No. 3 (2017): September 2017
Publisher : SANGKAREANG

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Abstract

Studi of interaction between Gyrinops versteegii and Fusarium Sp. could lead advance understanding in agarwood cultivation. The aim of this research is to observe interaction between Fusarium Sp. and Gyrinops versteegii based on morphological, anatomical and chemical observation. Gyrinops versteegii seedling from Lingsar was inoculated by Fusarium sp using grafting method. Morphological observation conducted by observation of chlorosis in leaf and dark colorization in stem. Anatomical observation conducted by observation of seedling stem tissue. Chemical observation conducted by thin layer chromatography. Based on morphological observation, chlorosis of leaf occurred after 14 days inoculation followed by necrosis of leaf at 21 days after inoculation. Penetration of fungal mycelia, fungal conidia and formation of dark coloration on seedling tissue were observed from anatomical observation. Similar spot with similar Rf (0.22) value were observe from seedling sample at 14 days inoculation, 21 days inoculation and also sample of Gyrinops versteegii 6 month after inoculation. All of those spots have similar Rf with standard agarwood oil (Rf = 0.23). Organoleptic test confirmed aromatic odor from seedling at 14 days and 21 day inoculation even though the aromatic odor is not as strong as aromatic odor of Gyrinops versteegii tree at 6 month inoculation. It could be concluded that the inoculation of Fusarium sp. on Gyrinops versteegii resulting: chlorosis and necrosis of leaf, dark colorization of stem, aromatic odor and identical Rf spot of Thin Layer Chromatography.
Co-Authors . Akram Aini, Husnul Akhmad Sukri Akram Akram, . Amelia Kandisa Anita Nugraheni, Yosephin Martha Maria Anri Anri Arbi Sanjaya Baharudin Baharudin Cep Ahmad Muhtar Dewi, Ni Putu Ety Diana Hidayati Efendi Iskandar Elly Purwanti Farouq Isbulloh Fathurrahman Fathurrahman Fatihatum, Fatihatum Gunawan, Frendi Hafiz, Yusron Hairani Hairani Hendriko, Albert Hidayah, Zoya Saharani Hidayat Wicaksono I Gde Dharma Atmaja I Gusti Agung Ayu Hari Triandini Irama, Ade Isbulloh, Farouq Isviyanti Isviyanti Kemas Usman Kesumajayadi, Lalu Kurnia, Nova Lutfi Anggadhania Lutfi Anggadhania Malik, Sukardi Masrilurrahman, LL. Suhirsan Mirawati, Baiq Nahlunnisa, Hafizah Ni Made Gita Gumangsari Ni Made Gita Gumangsari Nova Kurnia Putri, Dina Soes Putu Surya Wedra Lesmana Raden Roro Narwastu Dwi Rita Radjali Amin Rahma Ziska Rosa Martadiana Sad Kurniati Wanitaningsih Sad Kurniati Wanitaningsih Sad Kurniati Wanitaningsih Sadir, Muhammad Salman Al Farisi, Salman Al Sami'un Sarowan Wahid Sembiring, Rinawati Sukardi Malik Sumarlin, Muhamad Wahyu Prihanta Wahyu Yuniati Nizar Wanitaningsih, Sad Kurniati Wayan Wangiyana Wiranto Wiranto Wulandari, Febriana Wulandari, Febriana Tri Yani Mulyani Yayan Suherna Putri Yulia Ratnaningsih Yulia Ratnaningsih Yulia Ratnaningsih Zarkasi Nur