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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Biotechnology Biology, Medicine, & Natural Product Chemistry Majalah Obat Tradisional The Indonesian Biomedical Journal Majalah Farmaseutik Indonesian Journal of Chemistry Indonesian Contemporary Nursing Journal (ICON Journal) Indonesian Journal of Cardiology Journal of Community Empowerment for Health Devotion: Journal of Research and Community Service The Avicenna Medical Journal Indonesian Journal of Pharmacology and Therapy
Dwi Aris Agung Nugrahaningsih
*) Department Of Pharmacology And Therapy, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada; *) Herbal Medical Center, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health

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Acute irritation of tetracyclic c-4-hydroxyphenylcalics [4] pyrogalolarene on skin of albino rabbits Aditya Nugraha; Dwi Aris Agung Nugrahaningsih; Rul Afiyah Syarif; Mia Munawaroh Yuniyanti; Ikhsan Nur Salim; Ratna Sari; Jumina; Eti Nurwening Sholikhah
Indonesian Journal of Pharmacology and Therapy Vol 1 No 1 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.480

Abstract

C-4-hydroxyphenylcalix[4] pyrogalolarene (Pg4OH) is a synthetic chemical compound that shows strong antioxidant activity. It is potential to be developed as UV skin protector. For topical admission, an acute skin irritation test is being prerequisite to be fulfill. This study was conducted to investigate the irritation effect of Pg4OH on the skin. In vivo study by quasi experiment with posttest only design was carried out by employing 3 adult male albino rabbits. The back hair was shaved at 24 h before the treatment. The Pg4OH was smeared on the bare shaved-skin and left naturally within 4 h. The observation began at 24, 48 and 72 h after exposure. The erythema and edema scores were measured. Calculation by using primary irritation index (PII) was applied. Calculation was categorized with globally harmonized system (GHS) and international organization for standardization (ISO) parameters. As a result, among three rabbits, only one animal shown erythema and edema with very small size (score: 1), on 3 sites, at 72 h. Both the control site and the sample sites gained same score. Two other rabbits were obtained 0 value at each observation location. According to the PII, the observed data was obtained score 0.0093 (very light or negligible). It can be concluded that the tetracyclic compound of Pg4OH does not irritate the skin.
Expression of VEGF-A And COX-2 mRNA in non-steroidal anti-inflammatory drugs treated rat primary colonic fibroblast Andrew Limavanady; Jonathan J; Gloria Evita Thalia; Rasya Mayora; Ade Saputri; Rahmi Ayu Wijayaningsih; Eko Purnomo; Fara Silvia Yuliani; Woro Rukmi Pratiwi; Dwi Aris Agung Nugrahaningsih
Indonesian Journal of Pharmacology and Therapy Vol 1 No 1 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.508

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) is often used to shorten recovery time after surgery, including after colon anastomosis surgery. Studies showed that NSAIDs might involve in the development of colon anastomotic leakage. However, the effect of NSAIDs in colon anastomosis leakage is still a subject of controversy. Studies indicated that selectivity of COX-2 might have a role in the deleterious effect of NSAIDs in colon anastomosis. Disruption of VEGF-A by NSAIDs also suspected to be the culprit in the development of anastomosis leakage during NSAIDs treatment. This study aimed to investigate the NSAIDs effect toward VEGF-A and COX-2 mRNA in rat primary colonic fibroblast. The in vitro study was conducted using fibroblast isolated from rat colon. The isolated fibroblast was divided into 4 groups of treatment i.e.controlgroup, acetaminophen group, metamizole group, and ketorolacgroup. After 48 h of treatment, the cell was harvested and the RNA was isolated. The expression of VEGF-A and COX-2 mRNA was conducted using semi-quantitative PCR(sq-PCR). Both VEGF-A and COX-2 were not expressed in untreated rat colon fibroblast. However, VEGF-A mRNA washighly expressed in the ketorolacgroup. Interestingly, COX-2 mRNA couldbe seen in the ketorolac and metamizole groups but not in the acetaminophen group. The COX-2 mRNA expression wasthe highest in ketorolac treated rat colon fibroblast. It can be concluded that the effect of various kinds of NSAIDs towards VEGF-A and COX-2 mRNA expression of colon fibroblasts is different. This condition is duetotheir different inhibitory selectivity towards COX-1 and COX2.
Animal models in antihypertensive drug development research Dwi Aris Agung Nugrahaningsih; Mia Munawarroh Yuniyanti; Rahmi Ayu Wijayaningsih; Mosa Rini Nurul Hidayati; Setyo Purwono
Indonesian Journal of Pharmacology and Therapy Vol 1 No 2 (2020)
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.585

Abstract

Hypertension is one of the most common diseases in the world. However, its pathogenesis is not fully understood and its treatment is not yet satisfying. Animal models of hypertension have been useful to understand the pathogenesis of hypertension and to test novel therapeutic agents. There are several kinds of hypertension animal models. Each model has different characteristics. Knowing the characteristic of each model is important to obtain valid research. This review will describe several available methods to develop animal model for hypertension.
The comparison of colchicine and standard treatment in COVID-19 patients: A mini review Yasmeen Kwok; Dwi Aris Agung Nugrahaningsih; Woro Rukmi Pratiwi
Indonesian Journal of Pharmacology and Therapy Vol 2 No 3 (2021): Special Issue: COVID-19
Publisher : Faculty of Medicine, Public Health, and Nursing Universitas Gadjah Mada and Indonesian Pharmacologist Association or Ikatan Farmakologi Indonesia (IKAFARI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijpther.2381

Abstract

Coronavirus disease 2019 (COVID-19) was declared a pandemic in March 2020. There are several drugs used at different stages of the disease, but none seem to be completely effective. In the absence of any known therapy, many drugs have been tested, including colchicine. Colchicine differs from these other therapies in having pleiotropic mechanism of action, being less potent on any single target, and being an oral agent. It is also a better option since colchicine is not immunosuppressive, is not known to increase risk of infection, and is inexpensive. The aim of this study was to provide evidence of the role of colchicine in improving clinical outcome and reducing death rate of COVID-19 patients through literature mini-review. A mini-review, composed of previous RCTs comparing the use of colchicine in COVID-19 with standard care or placebo under the keyword “COVID-19”, “COVID”, “coronavirus”, “SARS-CoV-2”, “coronavirus disease”, “Colchicine”, “CAS 64-86-8”, “standard treatment”, “standard care”, “standard of care”, “common treatment”, “placebo”, “clinical improvement”, and “clinical response”. The addition of colchicine to standard treatment can be beneficial in treating COVID-19. Studies included showed that colchicine has significantly improved time to clinical deterioration compared to the control group. Colchicine reduced the length of hospitalization and the need for supplemental oxygen therapy. One of the included studies mentioned that in outpatient settings, colchicine can prevent hospitalization and even death. Colchicine can improve the clinical outcome on CRP levels, the number of patients that need mechanical ventilation, and death rate in COVID-19 patients. The addition of colchicine to standard treatment should be considered to prevent clinical deterioration and death. Colchicine reduces the number of adverse effects in COVID-19 patients except for gastrointestinal adverse effects, especially diarrhea, which is consistently more common in the colchicine groups.
The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Population: The Genetic Polymorphisms of CYP3A4*1G and CYP3A5*3 in Javanese Indonesian Atmaja, Sarah; Ellsya Angeline Rawar; Ani Kristiyani; Dwi Aris Agung Nugrahaningsih; Ahmad Hamim Sadewa; Christine Patramurti
Journal of Tropical Life Science Vol. 14 No. 1 (2024)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.14.01.06

Abstract

Polymorphisms of CYP3A4*1G and CYP3A5*3 affect the pharmacokinetic profile of various drugs, e.g., fentanyl, tacrolimus, diltiazem, simvastatin. Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR) is a simple and economical method for SNP determination. The polymorphisms in the CYP3A4*1G and CYP3A5*3 genes have not yet been examined using this method in Javanese Indonesian. Our aim was to determine the frequency of polymorphisms in the CYP3A4*1G and CYP3A5*3 genes in Indonesian Javanese using the ARMS-PCR method. Eighty-six patients at the Kalasan Community Health Centre in Yogyakarta, Indonesia, were chosen based on the inclusion criteria, which is Javanese ancestry. They gave their informed consent to blood collection by completing a form. Genetic variants were detected using Tetra-primer amplification refractory mutation system-polymerase chain (ARMS-PCR). The chi-square test was used to determine genotype deviations from Hardy-Weinberg equilibrium, with a significant threshold of 0.05. For homozygous wild types, CYP3A4 *1/*1 dominated overall among study participants (73.35%), whereas for CYP3A5*3/*3, homozygous mutants were more prevalent (83.72%). Hardy-Weinberg equilibrium is consistent with genotype frequencies (p > 0.005). One participant carried a homozygous mutation for both CYP3A4*1G and CYP3A5*3, while the other 49 subjects were heterozygous for CYP3A4*1G and homozygous mutant for CYP3A5*3, which is the highest number of SNP combinations. The findings of the current investigation demonstrate that the population has the highest proportion of homozygous CYP3A4*1G wild-types (CYP3A4*1/*1) and homozygous mutants for CYP3A5*3 (CYP3A5*3/*3)
Relationship between Lead Exposure and Genotoxic Effect in Paint Industry Workers Rahman, Intan Nur’azizah; Oginawati, Katharina; Ismawati, Yuyun; Buftheim, Sonia; Nugrahaningsih, Dwi Aris Agung
Devotion : Journal of Research and Community Service Vol. 4 No. 7 (2023): Devotion: Journal of Research and Community Service
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/devotion.v4i7.517

Abstract

Lead-based paint is a main source of lead exposure to paint industry workers and causes an imbalance of Reactive Oxygen Species (ROS) and antioxidants, causing a genotoxic effect. Pb in the blood (PbB) level and DNA damage are frequently used as exposure and effect biomarker of lead. The purpose of this study to determine the relationship between PbB level and DNA damage due to occupational lead exposure in paint industry workers. The research design uses a cross-sectional epidemiological study involving 52 workers from three paint manufacturers in Indonesia. Blood samples were taken for PbB analysis using ICP-MS, while DNA damage was analyzed using the Comet Assay method. The PbB average obtained was 4.36±1.60 µg.dL-1, where 17 workers (32.69%) exceeded the safe limit value of PbB (5 µg.dL-1). Meanwhile, the influential factors of PbB are the working period and alcohol consumption (p=0.029). The level of DNA damage was represented as Tail DNA (%), and the average was 9.62±0.19 %. All respondents in this study were categorized as under low damage (Class 2). There was no significant relationship between PbB and Tail DNA (%) and has a negative correlation (p=0.878; r=-0.022). The study concludes that there was no difference in Tail DNA (%) between PbB ≥ 5 µg.dL-1 and PbB < 5 µg.dL-1 (p=0.876). It means that lead exposure in this finding has not reached a level that can significantly cause DNA damage. However, it is necessary to monitor PbB levels in workers to minimize genotoxic or other effects.
Balb/C Mice Optimization as Triple Negative Breast Cancer ModelWith 4T1 Cells and 4T1 Cells 3D Culture Inoculation Methods Zafrullah, Umar Farooq; Mubarika Haryana, Sofia; Nugrahaningsih, Dwi Aris Agung; Satriyo, Pamungkas Bagus; Rocca, Matteo
The Avicenna Medical Journal Vol. 6 No. 1 (2025): The Avicenna Medical Journal
Publisher : Faculty of Medicine, UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/2rk7tt03

Abstract

Background: Triple Negative breast cancer (TNBC) is a subtype of breast cancer with negative expression of Progesterone Receptor (PR), Estrogen Receptor (ER), and Human Epidermal growth factor Receptor 2 (HER-2). Among other types of breast cancer, TNBC has a high rate of malignancy and recurrence, invasive, distant metastases, and resistance to conventional therapy. An optimal TNBC model is needed for drug discovery research in targeted therapy. However, optimizing the TNBC animal model by inoculating the 4T1 tumor cell line has a number of challenges and it is quite hard to be developed, especially in Indonesia. Methods: This quasi-experiment study used 28 Balb/c mice divided into four groups (7 mice respectively), group A (injected with basal medium), group B (injected with 4T1 cell), group C (injected with 4T1 cell + Geltrex), and group D (injected with 3D Culture 4T1 cell + Geltrex). After being injected, mice were followed up for 35 days. Once a week they had to be observed by measuring body weight and tumor volume. After 35 days, mice were sacrificed. Liver, lung, brain, and mammary gland were analyzed histopathologically with Hematoxylin-eosin (HE). Chi-square statistical analysis was done to compare intergroups. Results: Histopathologically, there was a group that found positive samples of Invasive Ductal Carcinoma (IDC)-like with a percentage of 28. 57% (2 out of 7), namely group C. Meanwhile, group B and D did not find any positive samples of TNBC, but in the mammary tissue, there was inflammation and epithelial hyperplasia. No distant metastases were found in the liver, lungs, and brain, however, there was inflammation in these secondary organs. Statistically, there was no significant difference between treatment groups with p-value = 0.41. Conclusion: The 4T1 cell inoculation method can produce IDC-like on HE with an incidence of 28.75% (2 of 7 individuals) in group C.
Evaluation of Forest Honey on the Proliferation and Migration of Dermal Fibroblasts under Hyperglycemic Conditions: An In Vitro Study Rizqi, Januar; Agustiningsih, Denny; Nugrahaningsih, Dwi Aris Agung
Biology, Medicine, & Natural Product Chemistry Vol 14, No 2 (2025)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2025.142.951-955

Abstract

This study aims to evaluate the effect of forest honey on fibroblast proliferation and migration under hyperglycemic conditions in vitro. The research method involved culturing primary fibroblasts in high-glucose DMEM (25 mM) and dividing them into five groups: standard control (standard medium), hyperglycemia control (high-glucose medium without treatment), 3% honey, 1.5% honey, and 0.75% honey. Proliferation was assessed by counting live cells (Trypan Blue staining/hemocytometer) at 24, 48, and 72 hours. Migration was measured using a scratch assay (0, 24, 48, 72 hours) and analyzed with ImageJ. The Shapiro–Wilk test was used for normality; data were analyzed with ANOVA followed by LSD or Kruskal–Wallis (p<0.05). Results showed that hyperglycemia decreased proliferation compared to the standard control. Treatment with 1.5% honey and 0.75% honey consistently increased proliferation compared to the hyperglycemic control at all time points (p<0.05), while 3% honey showed a relatively lower increase. The standard control group achieved 100% closure at 72 hours in the migration variable. The honey groups (0.75–3%) showed an increase compared to the hyperglycemic control, but the difference was not significant at 24–48 hours and approached significance at 72 hours (p=0.057). In conclusion, forest honey at a concentration of 1.5% can increase fibroblast proliferation in a hyperglycemic environment. The effect on migration requires confirmation with osmolality controls and more sensitive endpoints. These findings provide a biological basis for developing honey-based diabetic wound care adjuvants focusing on dose optimization and formulation standardization.
Co-Authors Abdul Karim Zulkarnain Ade Saputri Aditya Nugraha Ahmad Hamim Sadewa Ajib Diptyanusa Akhmad Kharis Nugroho Amira Fawwaz Tsabitah Andina Setyawati Andrew Limavanady Ani Kristiyani Ari Wibowo Astika Widy Utomo, Astika Widy Atmaja, Sarah Aurelia Priscilla Regita Putri Budi Mulyaningsih Budi Mulyaningsih Buftheim, Sonia Caroline Caroline Christantie Effendy Christine Patramurti Denny Agustiningsih Dimas Adhi Pradana Dwi Liliek Kusindarta Eko Purnomo Ellsya Angeline Rawar Emilia Vivi Arsita Erna Kristin Ernaningsih Ernaningsih Eti Nurwening Sholikhah Eti Nurwening Sholikhah Foe, Kuncoro Gloria Evita Thalia Hendy Wijaya Hera Nirwati Herzan Marjawan Hevi Wihadmadyatami I Made Andika Bara Kusuma Ikhsan Nur Salim Ismawati, Yuyun Iwan Dwiprahasto Januar Rizqi Jonathan J Jumina Jusak Nugraha Katharina Oginawati Ketut Shri Satya Wiwekananda Mae Sri Hartati Wahyuningsih Mahatma Sotya Bawono Mia Munawarroh Yuniyanti Mosa Rini Nurul Hidayati Mubarika Haryana, Sofia Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Ngatidjan Ngatidjan Nico Jafet Nilasari, Fita Nur Arfian Oryza Chrisantia Pamungkas Bagus Satriyo Prasetyastuti Prasetyastuti Pratiwi, Woro Rukmi Probosuseno Probosuseno Raden Sumiharto Rahman, Intan Nur’azizah Rahmi Ayu Wijayaningsih Rahmi Ayu Wijayaningsih Rasya Mayora Ratna Sari Rizki Awaluddin Rocca, Matteo Ronny Martien Rul Afiyah Syarif Sam Ibeneme Satriyo, Pamungkas Bagus Senny Yesery Esar Setyawan Bekti Wibowo Setyo Purwono Siswanto Siswanto Sitti Rahmah Umniyati Sitti Rahmah Umniyati Sofia Mubarika Haryana Srikanth Karnati Süleyman Ergün Tety Hartatik Titien Budhiaty Tri Baskoro Tunggul Satoto Widya Wasityastuti Wuryanto Hadinugroho Yahiddin Selian Yasmeen Kwok Yudy Tjahjono Yufita Ratnasari Wilianto Yuliani, Fara Silvia Yuniyanti, Mia Munawaroh Zafrullah, Umar Farooq