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Jurnal Riset Sains dan Kimia Terapan

jrskt
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Published by Universitas Negeri Jakarta

ISSN : 23028467 EISSN : 23030720 DOI : https://doi.org/10.21009/JRSKT.

Core Subject : Science,

Chemistry

Jurnal Riset Sains dan Kimia Terapan (JRSKT) is the journal for the publication of original studies and occational reviews in the field of science and applied chemistry. It is published electronically two times a year

Arjuna Subject : Kimia(semua kategori) - Kimia (Lain-Lain)

Articles 93 Documents

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Validasi Metode Analisis Fosfor pada Sayuran dengan Pereduksi Stannous Chloride menggunakan Spektrofotometri Uv-Vis Natasya, Aulia; Riyandari, Baiq Amelia
Jurnal Riset Sains dan Kimia Terapan Vol. 10 No. 2 (2024): Jurnal Riset Sains dan Kimia Terapan, Volume 10 Nomor 2, Desember 2024
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.102.06

Abstrak Analisis fosfor dalam sayuran secara spektrofotometri UV-Vis telah dilakukan. Penelitian ini bertujuan mendapatkan kondisi optimum untuk analisis fosfor secara spektrofotometri UV-Vis, mendapatkan informasi pereduksi yang optimal dan pengaplikasian metode analisis fosfor yang memenuhi parameter validasi metode. Penelitian diawali dengan menentukan kondisi optimum analisis fosfat dengan spektrofotometri UV-Vis menggunakan pereaksi ammonium molibdat dan direduksi dengan timah (II) klorida dalam lingkungan asam menggunakan asam klorida (HCl). Selanjutnya metode analisis divalidasi berdasarkan parameter validasi metode. Reaksi molibdenum biru menghasilkan senyawa kompleks heterofosfomolibdat yang memiliki panjang gelombang maksimum 729 nm. Hasil validasi metode analisis fosfat yang direduksi dengan timah (II) klorida memiliki linieritas metode dengan R2=0,9983 pada rentang 0 – 0,05 mg L-1 . Absorptivitas molar sebesar 2,093 x 105 L mol-1 cm-1 . Serta batas deteksi sebesar 1,41 x 10-3 mg L-1 dan batas kuantisasi sebesar 4,28 x 10-3 mg L-1 . Nilai presisi dinyatakan sebagai persen standar deviasi relatif %RSD pada jagung, brokoli dan buncis masing-masing sebesar 1,93%, 1,37% dan 1,25%. Perolehan kembali didapatkan pada masingmasing sampel sayuran berkisar 99,36% - 109,50%. Berdasarkan hasil penelitian menunjukan fosfat yang terkandung dalam sayuran jagung, brokoli dan buncis tidak melebihi baku mutu menurut Persatuan Ahli Gizi Indonesia (PERSAGI). Kata kunci: fosfor, timah (II) klorida, spektrofotometri UV-Vis Abstract Phosphorus analysis in vegetables using UV-Vis spectrophotometry has been carried out. This research aims to obtain optimum conditions for phosphorus analysis using UV-Vis spectrophotometry, obtaining optimal reducing information and application of phosphorus analysis methods that meet method validation parameters. The research began by determining the optimum conditions for phosphate analysis using UV-Vis spectrophotometry using reagents ammonium molybdate and reduced with tin (II) chloride in an acidic environment using hydrochloric acid (HCl). Next, the analysis method is validated based on the method validation parameters. The molybdenum blue reaction produces a heterophosphomolybdate complex compound which has a maximum wavelength 729 nm. The validation results of the analytical method for phosphate reduced with tin (II) chloride have method linearity with R2=0.9983 in the range 0 – 0.05 mg L-1 . The molar absorptivity is 2.093 x 105 L mol-1 cm-1. And the detection limit is 1.41 x 10-3 mg L-1 and a quantitation limit of 4.28 x 10-3 mg L-1. Precision values are expressed as percent relative standard deviation %RSD in corn, broccoli and beans respectively 1.93%, 1.37% and 1.25%. The recovery obtained for each vegetable sample ranged from 99.36 % - 109.50%. There are no phosphates contained in corn, broccoli and green beans exceeds quality standards according to the Persatuan Ahli Gizi Indonesia (PERSAGI) Keywords: phospore, tin (II) chloride, UV-Vis Spectrophotometry

Uji Efektivitas Metabolit Sekunder Ekstrak Daun Tin (Ficus Carica L.) Sebagai Formulasi Body Scrub Al Fayyadie, Kayyisa Luzza; Anggun Noviannisa; Asworo, Yuliana Dwi; Prameswari, Dhanti Cynthia
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.06

Buah tin merupakan buah dengan tekstur yang khas dan dikenal luas karena berbagai manfaatnya bagi kesehatan manusia. Salah satu bagian pada tanaman tin yaitu daun tin juga dapat dimanfaatkan karena memiliki kandungan antioksidan yang bermanfaat bagi tubuh manusia. Namun, tidak banyak dari kita yang mengetahui bahwa daun tin juga bermanfaat bagi kesehatan kulit sehingga pemanfaatannya dalam produk kecantikan seperti body scrub masih jarang dilakukan. Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder pada ekstrak daun tin dan pengaruh kandungan metabolit sekunder dari ekstrak daun tin terhadap formulasi body scrub. Metode yang digunakan dalam penelitian ini yaitu, metode eksperimental dengan desain penelitian Rancangan Acak Lengkap (RAL). Sampel dalam penelitian ini yaitu daun tin muda dan daun tin tua yang dikeringkan dengan beragam suhu. Data yang dikumpulkan pada penelitian ini diperoleh dari berat basah daun tin, berat kering daun tin, uji fitokimia, dan uji FTIR. Hasil yang diperoleh dalam penelitian ini menunjukkan bahwa kandungan metabolit sekunder yang terkandung dalam ekstrak daun tin adalah flavonoid dengan pereaksi Wilstater akibat terbentuknya warna kuning dan NaOH 10% mengubah hijau menjadi coklat dan Uji polifenol yaitu uji fenolat dan tanin dengan FeCl3 yang mengubah hijau menjadi hitam kehijauan yang berperan sebagai antioksidan utama dengan kadar sedang. Selain itu sampel daun tin tua dengan pengeringan suhu 45° yang menyusut paling banyak sebesar 45% merupakan sampel yang terbaik diantara 5 sampel lainnya untuk dijadikan ekstrak daun tin pada formulasi body scrub didikung oleh uji FTIR yang menunjukkan daerah frekuensi tertinggi pada ikatan gugus (C-H) dengan tipe senyawa fenol sebesar 3629.46 cm-1 yaitu sampel DT 4503 yang memiliki kandungan fenol tertinggi sebagai antioksidan bagi kulit.

Deteksi Vibrio parahaemolyticus Menggunakan Primer Gen toxR2 dengan Gradient Polymerase Chain Reaction Nurjayadi, Muktiningsih; Krisdawati, Ismaya; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Rahmawati, Atikah Nur; Fitriyanti, Anisa; Musie, Royna Rahma; Kurniadewi, Fera; Sukmawati, Dalia; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; Elenshasy, Hesham Ali
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.03

Makanan adalah kebutuhan vital, dengan kriteria utama adalah keamanan, kualitas, dan nilai gizi. Untuk memastikan keamanan makanan, diperlukan metode deteksi yang cepat dan akurat, terutama untuk mendeteksi bakteri patogen penyebab keracunan makanan. Vibrio parahaemolyticus merupakan bakteri patogen yang banyak ditemukan pada makanan laut. Penelitian ini bertujuan untuk mengembangkan metode deteksi cepat V. parahaemolyticus dengan menargetkan gen toxR2 menggunakan Gradient Polymerase Chain Reaction. Gen toxR dipilih karena fungsinya sebagai pengatur penting gen virulensi. Tahapan yang dilakukan meliputi desain primer, penyiapan sampel bakteri dari biakan murni, dan uji amplifikasi menggunakan PCR Gradien. Hasil uji amplifikasi menunjukan bahwa pasangan primer toxR2 berhasil mengamplifikasi pada suhu 58-62°C dengan dihasilkan pita berukuran 137 bp. Berdasarkan hasil tersebut, dapat disimpulkan bahwa PCR Gradien dengan primer toxR2 dapat diaplikasikan untuk mengembangkan alat deteksi V. parahaemolyticus dengan dilakukan uji lanjutan seperti uji konfirmasi, uji spesifisitas, uji sensitivitas, dan uji pada pangan menggunakan Real-Time Polymerase Chain Reaction.

In Vitro Evaluation of the Antibacterial Effect of Virgin Coconut Oil Against Pseudomonas aeruginosa Associated with Folliculitis Hati, Futi Kusuma; Rismaya, Rina; Aurellia, Dema Griseldis
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.05

Virgin Coconut Oil (VCO) is a pure coconut oil rich in lauric acid, giving it potential antibacterial properties. Previous studies have successfully produced a VCO using a combined fermentation and enzymatic method with Neurospora sitophila, Lactobacillus plantarum, and papain. Nevertheless, further investigation is required to determine the antibacterial efficacy of the VCO against Pseudomonas aeruginosa, a bacterium responsible for folliculitis. This study analyzed of the antibacterial activity of VCO and Hydrolyzed Virgin Coconut Oil (HVCO) against P. aeruginosa in vitro. VCO is obtained by fermenting coconut milk using N. sitophila, L. plantarum and papain, then VCO undergoes hydrolysis with the aid of Lipozyme TL IM to yield HVCO. Inhibition zone assays were conducted to assess the antibacterial activity of 100% VCO and HVCO at varying concentrations of 25%, 50%, and 100%. The largest inhibition zone was shown by 100% HVCO (9.5 ± 0.3 mm in diameter), which was statistically significantly different from the control, ampicillin trihydrate. The data suggested that HVCO has enhanced antibacterial effectiveness against P. aeruginosa, compared to VCO. This increased activity is attributed to its higher free fatty acids and monolaurin content.

Air Quality Analysis in the Vicinity of Tanjung Lesung Beach, Banten Widianti, Riskia Chandra; Syaifullah, Mhd Muslim; Hanifah, Sarina; Mulyanah, Annisa
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.02

Beaches are among the most popular recreational destinations for the public. This study aims to evaluate the air quality in the Tanjung Lesung coastal area, Pandeglang, Banten, by collecting air samples followed by laboratory analysis. The air pollutants measured in this study include Total Suspended Particulate (TSP), Sulfur Oxides (SOx), and volatile organic compounds such as Benzene, Toluene, and Xylene (BTX). Sampling methods employed were the impinger method for SOx, High Volume Air Sampler(HVAS) for TSP and Pb, and activated carbon and XAD adsorbents for BTX. The results showed that the concentration of TSP reached 421.08 µg/m³, exceeding the national ambient air quality standard of 230 µg/m³. The concentration of SOx was recorded at 0.7837 ppm, which remains within the permissible limit. Meanwhile, BTX compounds were not detected in the air samples, likely due to very low concentrations. These findings suggest that although the coastal air is relatively free from volatile organic compounds, a high level of particulate pollution was observed, which is suspected to originate from nearby industrial activities. Regular air quality monitoring is essential to ensure public health and maintain the comfort of tourist environments.

Column Study and Desorption Performance of Coffee Biomass-Calcium-Alginate Beads to Remove Pb²⁺ Ions from Aqueous Solutions Hanifah, Sarina; Rusnadi, Rusnadi; Stiawan, Elva; Widianti, Riskia Chandra
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.01

Heavy metal pollution, particularly by lead (Pb²⁺), poses a serious environmental threat and can significantly impact human health. This study aims to evaluate the performance of an adsorbent composed of a mixture of coffee waste and calcium alginate (Coffee-Calcium-Alginate/CCA beads) in removing Pb²⁺ ions through column experiments, as well as to assess the desorption efficiency and reusability of the adsorbent. Column studies were conducted at 1 ppm Pb2+ ions initial concentration, a flow rate of 2.2 mL/min, using a column with a length of 10 cm and a diameter of 7 mm to determine the breakthrough time of the adsorbate, while desorption was performed using HNO₃ to evaluate the efficiency of Pb²⁺ ions release and the potential for adsorbent reuse. The results demonstrated that the CCA adsorbent was able to retain high efficiency over two adsorption–desorption cycles, with desorption efficiencies of 96.35% and 86.64%. The desorption mechanism involves protonation of carboxylate groups and competition between H⁺ and Pb²⁺ ions at the active sites. Overall, CCA beads exhibit promising potential as an effective and reusable adsorbent for treating water contaminated with heavy metals. Future research could explore the effects of varying initial Pb²⁺ ions concentrations and column height to gain a more comprehensive understanding of desorption dynamics within the column system. Keywords: adsorption, coffee biomass, calcium alginate, Pb2+, leads

Studi Variasi Suhu dan Kosentrasi Larutan Asam Sitrat terhadap Karakteristik Silika Daun Tebu menggunakan Metode Leaching Abustiana, Rahma Lolita; Mirzan, Muhamad; Sosidi, Husain; Khairuddin; Ridhay, Ahmad; Jusman
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.04

Telah dilakukan penelitian tentang studi variasi suhu dan konsentrasi larutan asam sitrat terhadap karakteristik silika daun tebu menggunakan metode leaching dengan 3 variasi suhu 60ºC, 80ºC, 100ºC dan 3 variasi konsentrasi 8%, 10%, 12%. Metode yang dilakukan ialah preparasi daun tebu, sintesis silika daun tebu menggunakan metode leaching dengan asam sitrat, dan pembakaran daun tebu hasil leaching asam sitrat. Pengujian dilakukan untuk memperoleh silika murni. Tujuan dari penelitian ini adalah untuk mengetahui variasi suhu dan konsentrasi larutan asam sitrat terhadap karakteristik silika daun tebu yang diperoleh dengan metode leaching. Hasil penelitian diperoleh 3 analisis yaitu, untuk analisis XRD menunjukan didapatnya hasil struktur silika yang masih amorf. Analisis FTIR menunjukan gugus fungsi yang terbentuk yaitu -OH, Si-O-Si dan Si-O. Analisis SEM menunjukan bahwa semakin tinggi suhu leaching yang diberikan terhadap sampel maka ukuran butir semakin merata.

Potential of the Ethyl Acetate Extract of Kesambi (Schleichera oleosa) Bark as an Ointment-Based Treatment for Skin Diseases Against Staphylococcus aureus Juliandoz; Dzulhijar; Ningrum, Sekar Ayu; Situmeang, Boima; Yulianti, Nani; Bilangi, Nurhayati; Musa, Weny Junus Abdul; Rondonuwu, Mervina
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 2 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 2, Desember 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.112.03

One plant with potential to treat skin diseases is the Kesambi plant. Based on community experience in Cilegon, a decoction of Kesambi tree bark has been used to treat skin ailments, including itching, insect bites, and scabies. This research aims to reveal the potential of ethyl acetate extract from the tree bark as a standardized ointment preparation. Extraction was performed using the maceration method. Quality testing of the ointment included antibacterial, safety, stability, organoleptic, and bioactive compound content testing. The best formulation was formulation 1, with an antibacterial inhibition zone of 0.6-0.7 mm, classified as moderate. Increasing the amount of extract did not significantly affect antibacterial activity. The bioactive compound content in the ointment consisted of compounds from the groups of triterpenoids, steroids, phenolics, and flavonoids.

Chemical and Physical Assessment of Water Quality in the Panimbang River Estuary, Pandeglang, Indonesia Sari, Feni Mustika; Deliaz, Mochamad Fajar; Kurniawan, Akbar Rizqi; Ayuliansari, Yossy; Widianti, Riskia Chandra
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 2 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 2, Desember 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.112.06

The Panimbang River in Pandeglang, Indonesia, is widely used by residents for daily activities. This study analyzed water quality using physical parameters, including pH, temperature, dissolved oxygen (DO), Total Dissolved Solids (TDS), TSS (Total Suspended Solids), salinity, and alkalinity. Samples were taken around the estuary, which is surrounded by settlements. The results showed a pH of 4.50–6.49, below the standard of 6–9, indicating slightly acidic water. High temperatures, thought to be due to daytime sampling, affect DO. The average DO value was only 2.96 mg/L, well below the minimum threshold of 5 mg/L, indicating poor aeration. The average TDS is above 1000 ppm, indicating pollution from household waste and human activities. The average alkalinity of 384.976 mg/L CaCO₃ is within the safe range (30–500 mg/L), so it can stabilize pH. The water quality in the Panimbang River Estuary indicates environmental pressure from anthropogenic activities, resulting in slightly acidic water with low aeration and high solute content. However, adequate alkalinity still helps maintain the water's pH stability. This condition indicates the need for more intensive monitoring and waste management to protect estuarine ecosystems, which are vital to the community and the surrounding environment.

Synthesis of Nd-Pheophytin Complex and Its Hydrolysis into Nd-Pheophorbide from Katuk Leaves (Sauropus androgynous (L.) Merr) Novayanti, Fadia; Adhitiyawarman, Adhitiyawarman; rahmalia, winda
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 2 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 2, Desember 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.112.02

Pheophorbide, as a chlorophyll derivative, has potential as a complexing ligand for heavy metals, including lanthanides. The synthesis of Nd-pheophytin complex and its more polar Nd-pheophorbide (pheophytin’s hydrolyzed product) were investigated using pheophytin isolated from Katuk’s (Sauropus androgynus L. Merr.) leaves. This study aims to determine the optimal reaction conditions and the complex characteristics. Pheophytin ligand and Nd were complexed under two different reaction conditions: room temperature and reflux at 65 °C for 10 hours. The pheophytin starting material was obtained by preparative thin-layer chromatography (PTLC), yielding 0.02 g. UV-vis analysis revealed characteristic absorption bands at 404 nm (Soret band) and 666 nm (Q band) in methanol, while FTIR spectra confirmed the presence of functional groups corresponding to pheophytin. The Nd-pheophytin complex formation was indicated by hypsochromic shifts in UV-vis spectra, suggesting the Nd³⁺ complexing. Fluorescence spectra between pheophytin and Nd-pheophytin showed distinct emission patterns, with pheophytin exhibiting peaks at 662 and 722 nm, while Nd-pheophytin displayed peaks with shoulders at 654 and 714 nm. Hydrolysis of Nd-pheophytin using 1 M NaOH at pH 10 produced Nd-pheophorbide. TLC analysis showed a decrease in the Rf value of Nd-pheophytin to Nd-pheophorbide from 0.95 to 0.43, with tailing, attributed to the higher polarity of pheophorbide. These findings confirm the successful synthesis and hydrolysis of the Nd-pheophytin complex.

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Yussi Pratiwi

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jrskt@unj.ac.id

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Journal Mail Official
jrskt@unj.ac.id

Editorial Address
Universitas Negeri Jakarta

Location
Kota adm. jakarta timur,

Dki jakarta

INDONESIA