cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Arif Rahman Hikam
Contact Email
bioeksakta@gmail.com
Phone
+6285741954045
Journal Mail Official
bioeksakta@gmail.com
Editorial Address
Fakultas Biologi Universitas Jenderal Soedirman Jl. dr. Soeparno No. 63 Purwokerto, Kabupaten Banyumas Kode Pos 53122
Location
Kab. banyumas,
Jawa tengah
INDONESIA
BioEksakta : Jurnal Ilmiah Biologi Unsoed
Published by Universitas Jenderal Soedirman
ISSN : -     EISSN : 27148564     DOI : -
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary
Jurnal BioEksakta menerbitkan artikel ilmiah hasil penelitian dalam bidang biologi umum termasuk Ekologi dan konservasi, Taksonomi dan Struktur, Biogeografi, Evolusi, Biodeversitas, Fisiologi dan Reproduksi, Biologi sel, Biologi Molekuler dan Genetika.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 401 Documents
 27   28   29   30   31 
Uji Viabilitas Polen Tanaman Kedelai (Glycine max (L.) Merril) pada berbagai Lama Penyimpanan Andini, Hastya Tri; Abbas, Muachiroh; kamsinah, Kamsinah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4595

Abstract

Pollen can be used for plant identification because it has a distinctive structure and ornamentation, besides that it can also be used as a plant breeding agent as a contributor to male parental characters. The character of pollen as parental male is very important in plant breeding. One way to know the quality of pollen is to look at its viability. Pollen viability can be tested by staining method or by germination method. Pollen viability is known to be lost over a certain period of time. The purpose of this study was to determine the effect of storage time on viability of soybean pollen, and to determine the best storage period with the highest viability of soybean pollen. This research was conducted experimentally with a Completely Randomized Design (CRD) consisting of 4 treatments, namely P0, Control. P1, 7 days of storage. P2, 14 days of storage. P3, 21 days of storage. The independent variable is variation in storage time while the dependent variable is pollen viability. The parameters observed were the number of stained pollen, germination capacity, and the length of the germinating pollen tube as evidence that the pollen was viable. The research data obtained were analyzed using Analysis of Variance (ANOVA) with an error rate of 5%, then continued with the BNT test with an error rate of 5%. The results showed that the provision of storage time treatment on soybean pollen had an effect on pollen viability, namely by reducing pollen viability. The best shelf life to get the highest pollen viability in soybean pollen is 0 to 7 days after the sample is taken.
Optimasi Konsentrasi Glukosa dan Waktu Inkubasi Terhadap Bobot β-Glukan Jamur Schizophyllum commune Apriyani, Tri Rahayu; Ekowati, Nuraeni; Ratnaningtyas, Nuniek Ina
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4601

Abstract

Schizophyllum commune is a fungus that grows wild in nature which contains β-glucan which has the potential to develop drugs for several diseases and human health. The main purpose of this study was to determine the effect glucose concentration and incubation time on the growth of fungus S. commune and its β-glucan production. The research was conducted by experimental method of completely randomized factorial design (CRD factorial) with two factors carried out in three repetitions. The treatments provided included variations in glucose concentration (G) with three variations, namely 10 g/L (G1), 20 g/L (G2), and 30 g/L (G3), and variations in three different incubation times (W), such as 20 days (I1). , incubation time of 25 days (I2), and incubation time of 30 days (I3). Variables which used in this research independent and dependent variables. The independent variables were glucose concentration and incubation time, while the dependent variables were fungal mycelium growth and β-glucan production. The main parameter observed was β-glucan weight. The supporting parameters were dry biomass weight and the final pH medium. Data analysis was performed by Analysis of Variance (ANOVA) at 95% accuracy levels, followed by Duncan's test (Ducan Multiple Range Test). The results showed that glucose concentration and incubation time were significantly affected to the growth and production of β-glucan fungus S. commune. Glucose concentration of 30 g/L and incubation time of 25 days was the optimum condition for the growth of S. commune and its β-glucan production with an average dry weight of betaglucan is 0.363 g/L
Biodegradasi Bioplastik Berbasis Pati Menggunakan Isolat Fungi Indigenous Asal Tempat Pembuangan Akhir Gunung Tugel, Kabupaten Banyumas Fa'is, Jimmy Al; Dewi, Ratna Stia; Sari, Ajeng Arum
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 4 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.4.4605

Abstract

The use of plastic in Indonesia, especially as packaging, is very popular among the public, because it is practical and has many uses. Plastic consumption in 2020 even reached 67.8 million tons per day. Therefore, one way to overcome this problem is to use and produce biodegradable plastics or bioplastics. Biodegradation of bioplastics can be done with fungi. The potential place to find bioplastic degrading fungi is the Gunung Tugel Ex-Final Disposal Site (TPA). Considering the fact that bioplastics have a fairly good level of degradation, in this study a starchbased biodegradation test will be carried out using isolates of indigenous fungi originating from Ex-TPA Gunung Tugel using the method of calculating the weight loss of bioplastics (weight loss). This study aims to obtain starch-based bioplastic degrading fungi isolates from Ex-TPA Gunung Tugel, Banyumas Regency and determine the highest biodegradability ability of starchbased bioplastics by fungal isolates from Ex-TPA Gunung Tugel, Banyumas Regency. The results showed that isolates of indigenous fungi that had the potential to degrade starch-based bioplastics from Ex-TPA Gunung Tugel, Banyumas Regency were obtained from the genus Aspergillus (GT2 isolate) and Penicillium (GT1, GT3, GT4, GT5, and GT6) isolate. The highest biodegradability of starch-based bioplastics was found by fungi from the genus Aspergillus, namely, isolate GT2 with a weight loss of 21.84%. The biodegradation occurs due to the activity of endoamylase, exoamylase, and glucanase enzymes produced by fungi. The observation of the texture of the bioplastic resulted in a significant change in color, namely from the white bioplastic sheet to brownish. These observations also showed the attachment of fungal mycelium on the surface of the bioplastic accompanied by a hollow and uneven surface of the bioplastic. Key words: Biodegradation, Starch-based bioplastic, Indigenous Fungi, TPA.
Histopatologi Hepar Mencit (Mus musculus) Jantan yang Diberi Air Rebusan Plastik terhadap Antioksidan Alami Safitri, Nuridha Audinia; Atang, Atang; Wibowo, Eko Setio
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4606

Abstract

Plastic in Indonesian daily life cannot be avoided anymore. Materials for the manufacture of plastic can cause free radicals to the body, thus affecting the immune system. These materials are including phthalates and bisphenol-A (BPA), which commonly called as plasticizers. Free radicals in the body can be inhibited with antioxidants. Antioxidants can be obtained from endogenous (inside the body) and exogenous (outside the body). The purpose of this study was to determine the damage level of liver histopathological structure as the result of plastic boiled water induction towards natural antioxidants feeding on male mice (Mus musculus). This study used an experimental method with a completely randomized design (CRD) consisting of six treatments and five replications for 21 days. The treatments included two control groups and four other groups treated with plastic boiled water and natural antioxidants. The independent variable is natural antioxidants and the dependent variable is the liver histopathology of male mice. The qualitative parameters observed were the liver histopathological structure of male mice is in the state of inflammatory cell infiltration, degeneration, and necrosis. The research data is an overview of the liver histopathological structure of male mice. The obtained data were analyzed using descriptive qualitative analysis method. Oral induction of boiled-plastic water can affect the changes of liver histological structure. Natural antioxidants given in male mice can maintain the tissue structure as the result of boiled-plastic water induction. The results showed the damage level of male mice liver histopathological structure as reversible degeneration.
Pengaruh pH dan Suhu terhadap Aktivitas Enzim Protease Isolat LG-37 Asal Sedimen Mangrove Pantai Logending Kebumen Sari, Dhea Nur Khomala; Kusharyati, Dyah Fitri; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 1 (2024): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.1.4674

Abstract

Enzim protease merupakan enzim yang berfungsi menghidrolisis protein menjadi peptida dan asam amino. Setiap enzim memiliki kondisi atau aktivitas optimal berbeda-beda. Aktivitas enzim dipengaruhi oleh berbagai faktor diantaranya adalah pH dan suhu. Penelitian ini bertujuan untuk mengetahui pengaruh pH, suhu, dan kombinasi keduanya terhadap aktivitas enzim protease dari isolat LG-37 asal sedimen mangrove Pantai Logending. Metode penelitian dilakukan secara eksperimental menggunakan rancangan acak lengkap (RAL) pola faktorial dengan dua faktor. Masing-masing faktor tersebut dilakukan pengulangan tiga kali. Faktor pertama yakni variasi pH 4, 5, dan 6, sedangkan faktor kedua yakni variasi suhu 45, 50, dan 55oC. Hasil penelitian menujukkan bahwa pH dan suhu berpengaruh terhadap aktivitas enzim protease isolat LG-37 asal sedimen mangrove Pantai Logending. Aktivitas protease tertinggi diperoleh pada perlakuan kombinasi pH 6 dan suhu 50oC dengan nilai unit aktivitas protease sebesar 1,067 U/mL.
Keanekaragaman Morfologi Citrus spp. di Kecamatan Ajibarang Aziztia, Rahayu Nafisah; Herawati, Wiwik; Hidayah, Hexa Apriliana
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 3 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.3.4717

Abstract

Jeruk (Citrus spp.) merupakan salah satu komoditas yang menjadi buah andalan Nasional Indonesia dari sepuluh tanaman hortikultura lainnya yang didasarkan dari potensi keanekaragaman jeruk yang tinggi di Indonesia. Jeruk dapat dijumpai dalam setiap musim karena tanaman jeruk termasuk mudah dan cocok di berbagai kondisi iklim, dapat ditanam dimana saja, baik di dataran rendah maupun di dataran tinggi. Tanaman jeruk termasuk dalam famili Rutaceae yang terdiri dari 150 genus. Tanaman jeruk mempunyai 16 spesies namun varietasnya cukup banyak. Keanekaragaman genetik tanaman jeruk tersebut dapat dipelajari antara lain melalui karakteristik morfologi. Penelitian ini dilakukan untuk mengetahui keanekaragaman Citrus spp. yang terdapat di Kecamatan Ajibarang dan mengetahui hubungan kemiripan spesies jeruk yang ditemukan di Kecamatan Ajibarang. Berdasarkan hasil penelitian di 5 desa di Kecamatan Ajibarang ada 7 spesies tanaman jeruk (Citrus spp.) yaitu jeruk bali (C. maxima), jeruk lemon (C. limon), jeruk purut (C. hystrix), jeruk katsuri/kalamansi (C. mitis), jeruk nipis (C. aurantifolia), jeruk siam (C. nobilis), dan jeruk manis (C. sinensis). Spesies yang memiliki hubungan kemiripan terdekat yaitu C. mitis dan C. nobilis dengan indeks disimilaritas yaitu 0,416. Sedangkan spesies yang memiliki hubungan kemiripan terjauh yaitu C. mitis dan C. maxima dengan indeks disimilaritas 2,057.
Characteristics and Antibacterial Activities of Bacteriocin Produced by Lactid Acid Bacteria Isolate LG-50 and LG-90 Putri, Thalitha Nindika Nathania; Kusharyati, Dyah Fitri; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 3 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.3.4721

Abstract

Biopreservative agents is natural food preservative agent derived from microbes such as bacteriocin produced by lactic acid bacteria (LAB). Bacteriocin is a protein compound that has a small molecular weight and has antibacterial activity because it is effective in preventing the growth of pathogenic bacteria in food and beverages. Escherichia coli and Staphylococcus aureus are pathogenic bacteria that can contaminate seafood products because they can cause infection and food toxication. Isolates LG-50 and LG-90 are LAB, bacterial collection of the Microbiology Laboratory, Faculty of Biology, Isolate from mangrove sediment in Logending beach. Both isolates are not yet known for their potencies to synthesize bacteriocin and its inhibitory capability to the growth E. coli and S. aureus. The research aimed to determine the ability of LG-50 and LG-90 isolates to produce bacteriocin, to determine the ability of LG-50 and LG-90 isolates to inhibit the growth of E. coli and S. aureus bacteria invitro, to identity the characteristics of isolate LG-50 and LG-90 from Logending beach mangrove sediment. The results of this research show that the bacteriocins LG-50 and LG-90 were able to inhibit the growth of E.coli with inhibition zone diameters of 13.5 mm and 13 mm, in S. aureus with inhibition zone diameters of 15 mm and 15.5 mm.
Karakterisasi dan Pengaruh Senyawa Antibakteri Streptomyces spp. dalam Menghambat Pertumbuhan Vibrio cholerae Andani, Ajeng Putri Retno; Oedjijono, Oedjijono; Ryandini, Dini
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 4 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.4.4726

Abstract

Penelitian ini bertujuan untuk mengetahui kemampuan Streptomyces spp. yang diisolasi dari sedimen mangrove dalam menghambat pertumbuhan bakteri Vibrio cholerae, mengetahui kekuatan senyawa antibakteri yang dihasilkan oleh Streptomyces spp. terhadap V. cholerae, dan mengetahui karakteristik dari senyawa antibakteri yang dihasilkan oleh Streptomyces spp. Tahap penelitian meliputi penapisan isolat bakteri Streptomyces spp. (SA34, SA37, SA40, SAE4034) yang menghambat V. cholerae, produksi senyawa antibakteri, uji aktivitas antibakteri dengan metode Kirby Bauer, penentuan nilai MIC (%), pemisahan senyawa antibakteri dengan metode TLC, uji fitokimia, uji bioautografi, uji aktivitas senyawa antibakteri pada variasi suhu (40oC, 60oC. 80oC, 100oC) dan pH (2, 4, 6, 9), serta uji konfirmasi karakter bakteri. Data dianalisis secara deskriptif. Hasil penapisan menunjukkan bahwa penghambatan terhadap pertumbuhan V. cholerae tertinggi ditunjukkan oleh isolat SAE4034. Ekstrak kasar mampu menghambat V. cholerae dengan diameter zona hambat 11,5-17,5 mm; nilai MIC 30%, dan menghasilkan senyawa bioaktif dengan nilai Rf berkisar antara 0,31-0,70. Senyawa yang mampu menghambat memiliki nilai Rf 0,43 dan senyawa tersebut termasuk golongan alkaloid. Perlakuan suhu 40oC tetap tinggi dengan zona hambat 14 mm dan aktivitas penghambatannya menurun sejalan dengan perlakuan suhu yang lebih tinggi sampai 100oC. Perlakuan pH 6 menunjukkan aktivitas penghambatan tertinggi dengan zona hambat 16 mm, sebaliknya pada pH 2, 4, dan 9 aktivitas antibakteri ekstrak kasar menurun.
Potensi Fungi Asal Air Sungai Mengaji dan Prukut Kabupaten Banyumas sebagai Biodegradasi Pestisida Ayunda, Batari Citra; Dewi, Ratna Stia; Sastranegara, Moh Husein
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 5 No 4 (2023): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2023.5.4.4732

Abstract

Pestisida merupakan zat kimia yang digunakan oleh bidang pertanian untuk melindungi tanaman dari serangan hama. Namun, kehadiran pestisida memiliki dampak negatif bagi lingkungan, kesehatan manusia, dan organisme yang hidup di sekitar tempat yang terpapar pestisida secara jangka panjang. Pada lahan pertanian, penggunaan pestisida menyebabkan residu pestisida terakumulasi di air sungai. Sehingga hanya organisme tertentu saja yang dapat bertahan hidup, salah satunya fungi. Penelitian menggunakan air sungai yang diambil sebagai sampel dan dilakukan isolasi untuk mendapatkan fungi yang berpotensi untuk mendegradasi pestisida. Uji potensi fungi dilakukan dengan penanaman fungi hasil isolasi dan pemurnian ke campuran media PDA (Potato Dextrose Agar) dan klorpirifos, pengukuran diamater koloni yang tumbuh pada media tersebut, dan identifikasi fungi sampai tahap genus yang memiliki potensi besar dalam mendegradasi pestisida. Lokasi pengambilan sampel berada di enam titik stasiun Sungai Mengaji dan Prukut Kabupaten Banyumas. Lokasi analisis sampel berada di Laboratorium Mikologi dan Fitopatologi Fakultas Biologi Universitas Jenderal Soedirman. Tujuan dilakukannya penelitian ini adalah memperoleh isolat fungi dari sungai Mengaji dan Prukut Kabupaten Banyumas, mendeteksi kemampuan fungi dalam mendegradasi pestisida, dan mengetahui genus isolat terbaik dalam mendegradasi pestisida. Isolat fungi yang diperoleh dari keenam stasiun Sungai Mengaji dan Prukut Kabupaten Banyumas terdiri dari 25 isolat yaitu F1, F2, F3, F4, F5, F6, F7, F8, F9, F10, F11, F12, F13, F14, F15, F16, F17, F18, F19, F20, F21, F22, F23, F24, dan F25. Isolat fungi asal Sungai Mengaji dan Prukut Kabupaten Banyumas yang memiliki kemampuan tinggi dalam mendegradasi pestisida antara lain F12, F21, F24, dan F25. Genus isolat terbaik dalam mendegradasi pestisida setelah dilakukan identifikasi secara makroskopis dan mikroskopis adalah Trichoderma sp.
Identifikasi dan Uji Potensi Amilolitik Isolat Jamur Pendegradasi Sampah Organik Haqq, Insaaniy Mahdiyatul; Dewi, Ratna Stia; Mumpuni, Aris; Hikam, Arif Rahman; Yulianti, Dwiana Muflihah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 1 (2022): Bioeksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.1.4748

Abstract

Organic waste is composed of organic compounds. The accumulation of organic waste is a serious problem. Fungal have an important role in degrading organic waste in the composting process. Biodegradation of organic waste is closely related to fungal ability to hydrolyze starch. The purpose of this study was to know fungi isolate which hydrolyze starch from organic waste and amylolytic potential of the isolate. This research was conducted by survey and experimental method. Kitchen waste samples consist of food waste and other organic waste taken from homes in Bancarkembar, Bobosan, Grendeng, Karangwangkal, Pabuaran, Purwanegara, and Sumampir village. A screening test with Starch Agar medium was used to know amylolytic potential of the isolates. Result showed there were eight isolates which have potential to hydrolyze starch. Six isolates which have higher amylolitic index were identified as Fusarium sp., Aspergillus sp., and Penicillium sp. Furthermore, to determine the amylolytic activity quantitatively, the DNS method was used to measure glucose levels. Fusarium sp. had the highest starch degradation activity with the average glucose content of the medium Fusarium sp. as much as 3,568.63 ppm.

Page 29 of 41 | Total Record : 401

 27   28   29   30   31 

Filter by Year

2019 2026


Reset
Filter By Issues
All Issue Vol 8 No 1 (2026): BioEksakta Vol 7 No 4 (2025): BioEksakta Vol 7 No 3 (2025): BioEksakta Vol 7 No 2 (2025): BioEksakta Vol 7 No 1 (2025): BioEksakta Vol 6 No 4 (2024): BioEksakta Vol 6 No 3 (2024): BioEksakta Vol 6 No 2 (2024): Bioeksakta Vol 6 No 1 (2024): Bioeksakta Vol 5 No 4 (2023): BioEksakta Vol 5 No 3 (2023): BioEksakta Vol 5 No 2 (2023): BioEksakta Vol 5 No 1 (2023): BioEksakta Vol 4 No 3 (2022): BioEksakta Vol 4 No 2 (2022): BioEksakta Vol 4 No 1 (2022): Bioeksakta Vol 3 No 4 (2021): BioEksakta Vol 3 No 3 (2021): BioEksakta Vol 3 No 2 (2021): BioEksakta Vol 3 No 1 (2021): BioEksakta Vol 2 No 4 (2020): BioEksakta Vol 2 No 3 (2020): BioEksakta Vol 2 No 2 (2020): BioEksakta Vol 2 No 1 (2020): BioEksakta Vol 1 No 2 (2019): BioEksakta More Issue