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Anakes: Jurnal Ilmiah Analis Kesehatan
Published by Universitas Mohammad Husni Thamrin
ISSN : 20885687     EISSN : 27456099     DOI : 10.37012/anakes
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology
Jurnal Ilmiah Analis Kesehatan merupakan jurnal yang berisi tentang artikel ilmiah dalam bidang ilmu analis kesehatan yang dilakukan oleh dosen maupun mahasiswa serta para peneliti.
Arjuna Subject : Profesi Kesehatan - Profesi Kesehatan (Lain-Lain)
Articles 311 Documents
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Identifikasi Jamur Penyebab Onikomikosis Pada Kuku Jari Kaki Petani Sawah Di Desa Rajeg Kabupaten Tangerang Mulyati; Rohadatul Aisy, Dzia Ulhaq; Wulandari, Destiana
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.1995

Abstract

Fungi are eukaryotic microorganisms that can grow in damp places. Onychomycosis is a fungal infection of the nails, which causes nail damage affecting approximately 10% of the world's population. Rice farming is a job that has a high risk of onychomycosis due to high activity in a humid environment. The aim of this research was to determine the description of the fungus that causes onychomycosis in the toenails of rice farmers in Rajeg Village, Tangerang Regency.This research uses a descriptive observation method with data analysis presented in the form of univariate and bivariate tables. The research was carried out in May-June 2023, with 34 samples taken using the technique random sampling. The research results showed that the most positive results for onychomycosis infection occurred in rice farmers with female gender at 55.9% and the productive age group 26-65 years at 64.7%. Apart from that, onychomycosis infections often occur in farmers who have worked >5 years, amounting to 82.3%, with the habit of not using footwear when working at 91.2% and farmers who do not wash their toenails with soap at 64.7%. Based on research, it is known that the frequency distribution of the fungus that causes onychomycosis in rice farmers by direct examination was found to be 41.2% and by culture examination was found to be 91.2% with the largest species found, namelyAspergillus sp 46.7%. Based on the research results, it can be concluded that length of work, habit of using footwear, and habit of washing toenails with soap are several factors that influence the occurrence of onychomycosis infection in farmers.   Keywords           : Fungi, Onychomycosis, Rice Field Farmers
Optimasi Metode Pemeriksaan HbA1c Secara Elektrokimia Berbasis Screen Printed Carbon Electrode Furqon, Ayi; Kania, Prina Puspa
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2314

Abstract

The quantification of HbA1c as an analyte used to monitor the success of diabetes treatment is generally examined in a laboratory with sophisticated technological equipment and professional staff. Point of care testing (POCT) is generally designed for practical examination at the patient's side. POCT HbA1c uses a screen-printed electrode (SPCE) reagent strip labeled with aptamer molecular imprinted polymer (MIP), boronate, or anti-HbA1c antibody and then quantified electrochemically. The linearity of the measurement works at a normal detection concentration of 5.5-8.5% but has not been tested at high critical concentrations. The purpose of this study was to optimize the electrochemical quantification method of HbA1c. The research procedure, SPCE was first activated with 0.1 M NaOH by cyclic voltammetry. Aptamer labeling (Fe3O4@MIPHbA1c) was carried out using the chronoamperometric technique and quantification was determined by the differential pulse voltammetry (DPV) technique. HbA1c stock of 6% and 10% in PBS pH 7.4 was used as normal and pathological concentration solution in accuracy and precision study (target quality CV <3%). The result, the average normal concentration of HbA1c was 5.9% ± 0.1%, CV=1.69% and pathological concentration was 10.4% ± 0.21%, CV=1.92%. The linearity test result of measurement ranged from 2%-15% R2=0.987. In conclusion, MIPHbA1c-activated SPCE showed optimal condition in HbA1c measurement in the normal and pathological range. Keywords: SPCE, HbA1c POCT, HbA1c Magnetit, HbA1c Aptamer
Skrining Talasemia Minor Pada Pendonor di Unit Donor Darah Palang Merah Indonesia Kabupaten Majalengka Winengsih, Wiwin; Novilla, Arina; Ningrum, Nining Ratna; Khairinisa, Gina
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2330

Abstract

Thalassemia is a hereditary hemolytic disease caused by a disorder in the synthesis of hemoglobin within red blood cells. Thalassemia prevention programs are beneficial for reducing or breaking the chain of thalassemia by preventing marriages between thalassemia carriers, which could result in thalassemia sufferers. One preventive measure is thalassemia screening. This study aims to identify the presence of thalassemia minor carriers among donors at the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. The study is a descriptive quantitative research with a sample size of 92 donors who visited the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. The thalassemia screening included hemoglobin tests, osmotic fragility tests, and erythrocyte indices. The results showed that all 92 donors had normal hemoglobin levels with an osmotic fragility test showing negative results using OTOF method. Therefore, further examination of the erythrocyte index was not conducted.. Based on these results, it can be concluded that there were no thalassemia minor carriers among the donors at the Indonesian Red Cross Blood Donation Unit in Majalengka Regency. Keywords  :Thalassemia, Donor,Osmotic Fragility, Hemoglobin
Gambaran Indeks Eritrosit Pada Penderita Tuberkulosis Paru di Rumah Sakit Umum Mary Cileungsi Bogor Kristianingsih, Yuli; Rahayu, Cahyawati; Prakoso, Aryo; Pratiwi, Cindy Dui
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2370

Abstract

Tuberculosis is a chronic disorder that spreads to several organs. It is caused by bacteria (Mycobacterium tuberculosis), which primarily affects the lungs. Cases remain high in the community, making it a global health issue. Tuberculosis can cause hematological disorders, affecting hematopoietic progenitor cells and plasma components, particularly erythrocytes. The examination of erythrocyte indices in pulmonary tuberculosis patients aims to determine the type of anemia. The purpose of this study is to describe the erythrocyte index values in pulmonary tuberculosis patients at Mary Cileungsi General Hospital, Bogor. This study is quantitative research using secondary data collected from medical records and then processed. The study involved 80 tuberculosis patients. The results showed that 48 patients (60%) had normal MCV, 30 patients (37%) had low MCV, and 2 patients (3%) had high MCV. For MCH, 48 patients (60%) had normal values, 30 patients (37%) had low values, and 2 patients (3%) had high values. Regarding MCHC, 69 patients (86%) had normal values, 8 patients (10%) had low values, and 3 patients (4%) had high values. The study concluded that there was an increase in erythrocyte indices in tuberculosis patients, both male and female, with an increase across all age groups. It is recommended to take medication regularly to reduce the risk of transmission. Additionally, this information can be useful in conducting erythrocyte index examinations to detect and prevent early indications of anemia. Keywords : Anemia, Erythrocyte index values, Pulmonary tuberculosis patients
Gambaran Kualitas Mikrobiologi Es Batu Kristal Dan Es Batu Balok Pada Minuman Di Pujasera Daerah Stasiun Kereta Api Duren Kalibata Rizka, Devriola Betty Gayu; Rahayu, Cahyawati; Kristianingsih, Yuli
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2374

Abstract

Waterborne diseases are infectious diseases that are directly caused by water. An example of a common disease is caused by the bacterium Escherichia coli. Diarrhea can be transmitted through drinking water contaminated with Escherichia coli. Water is one of the sources of life for needs and for consumption (drinking), one of the water used is in the form of ice cubes. Ice cubes have a variety of benefits, one of the benefits is as a complement that is served along with drinks, the addition of ice cubes in drinks is to cause a cold and fresh sensation. Many beverage traders use block ice cubes that are not for their designation. Ice cubes for consumption must be safe from coliform bacteria and Escherichia coli in accordance with the quality standards of PERMENKES NO.2 of 2023. The purpose of this study was to obtain a description of the number of coliform bacteria and Escherichia coli in crystal ice cubes and block ice cubes used by beverage sellers in the Pujasera area of Duren Kalibata Railway Station. This study uses an exploratory descriptive method. The population of this study were beverage sellers who used crystal ice cubes as many as 10 people and ice cubes as many as 8 people in the Duren Kalibata Railway Station Area Food Court with a sample of 8 people each and 8 crystal ice cubes and 8 crystal ice cubes. The results showed that all samples of crystal ice cubes and block ice cubes were positive for Coliform Bacteria, 60% of block ice cubes were positive for Escherichia coli, while 80% of crystal ice cubes were positive for Escherichia coli. This is due to the lack of knowledge, attitudes and actions of traders about the quality of ice cubes that must be maintained. It can be concluded that crystal ice cubes and block ice cubes sold at the Duren Kalibata Railway Station Area Food Court do not meet the requirements of Environmental Quality Standards. For traders and the public to pay attention to the hygienic quality of snacks or drinks that are sold and consumed.  Keywords           : Block ice cubes, Crystal ice cubes, Beverages, Microbiological Quality
Gambaran Kadar Elektrolit Darah (Natrium, Kalium, Klorida) Pada Pasien Balita Usia 0-5 Tahun dengan Diare Akut Di RSAB Harapan Kita Jakarta Nurdiani, Catu Umirestu; Sari, Indah Novitha Fitria; Nugroho, Heru Purwanto
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2368

Abstract

Diarrhea is an increase in the frequency of defecation, which is characterized by a stool consistency that is thinner than usual. Viruses, bacteria, and parasites can cause diarrhea. Diarrhea is the second most common cause of death in toddlers. The main cause of death in toddler diarrhea is severe dehydration due to excessive fluid loss. Laboratory tests on diarrhea patients include blood electrolyte tests including sodium, potassium, and chloride. This quantitative research is conducted with secondary data obtained from medical records and then processed. The study was conducted on all 64 patients aged 0-5 years with acute diarrhea at RSAB Harapan Kita. The results showed that based on gender, it was dominated by males with the highest abnormal electrolyte levels in chloride at 34 people (53.13%) with the lowest chloride value of 81 mmol/L and the highest chloride value of 125 mmol/L. Based on age, most diarrhea patients were found to occur in toddlers (1-5 years) with the highest abnormal electrolyte levels in chloride at 29 people (45.31%) with the lowest chloride value of 81 mmol/L and the highest chloride value of 114 mmol. /L. The research that has been carried out can conclude that in patients suffering from diarrhea, the average value of the patient's sodium and potassium levels is normal, while the chloride levels are high. It is hoped that it is important for parents of diarrhea sufferers to always maintain a clean environment to prevent diarrhea and immediately go to the nearest doctor or hospital when the body shows symptoms such as fever and continuous defecation.   Keywords: Diarrhea, Sodium, Potassium, Chloride
Neuroprotective Effect of Red Betel (Piper crocatum) Extract on Brain Monosodium Gluatame (MSG)-Induced Rat (Rattus norvegicus) Baihaki, Ichwan; Mutmainnah
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2373

Abstract

Indonesian people prefer to eat fast food which is generally rich in flavorings. The most common ingredient found in fast food is MSG (monosodium glutamate). MSG consumption that exceeds the limit will produce free glutamate which is excitotoxic in various organs, one of which is the neurological system. Red betel has a fairly high antioxidant content which can inhibit excitotoxicity by inhibiting the occurrence of free radicals. The aim of this research was to determine the neuroprotective effect of red betel extract (Piper crocatum) on the brains of rats (Rattus norvegicus) given monosodium glutamate (MSG). This research is a laboratory experimental study with a completely randomized design method using 5 treatment levels, namely negative control (NC), positive control (PC), treatment 1 (T1), treatment 2 (T2), treatment 3 (T3). The NC treatment level was given 2 mL of distilled water. The PC treatment level was given 7gr/KgBB MSG. Levels T1, T2, T3 were each given MSG 7gr/KgBW in the morning, then after 8 hours, level P1 was given 200mg/KgBW of red betel extract, P2 was given 400mg/KgBW of red betel extract, and P3 was given 600mg/KgBW of red betel extract. Examination of the number of neurons in the dentate gyrus of the hippocampus using the ImageJ application and examination of calcium ion levels using the RAPIDLab 348X tool. Data analysis used the One Way Anova test with the SPSS 24 program. The results showed that there was a significant difference (p<0.05) between PC and NC. The results of the calcium ion parameters showed that only the 600mg dose treatment group functioned as a protector in the occurrence of hypocalcemia, while the histopathology parameters showed that the 400mg and 600mg dose treatment groups functioned significantly as a neuroprotector.
Pengembangan Metode Identifikasi Kadar Ion Besi Sampel Urin Berbasis Separasi Magnetik Permana, Ellsie Viendra; Khristian, Erick; Oktaviani, Nidya; Muhammad Rizaldi Ridwan; Siti, Salma; Maharani, Devi
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2457

Abstract

Kelebihan zat besi (iron overload) dapat disebabkan oleh berbagai faktor, termasuk gangguan genetik seperti hemokromatosis herediter dan penyebab sekunder seperti transfusi darah berulang atau asupan besi yang berlebihan, yang sering terjadi pada pasien thalasemia. Penelitian ini bertujuan untuk mengembangkan metode baru dalam menentukan kadar ion besi dalam urin. Beberapa senyawa pembentuk presipitat ion besi yang diidentifikasi meliputi sulfit, peroksida, dan hidroksida, dengan hasil optimum diperoleh dari presipitasi menggunakan hidroksida berdasarkan parameter kestabilan dan kecepatan pengendapan dengan magnet. Sebelum analisis, sampel urin diperlakukan dengan penambahan magnesium untuk menghindari gangguan dari presipitasi ion fosfat. Hasil pengendapan optimum menggunakan hidroksida dicapai pada menit ketiga untuk ion besi dengan kadar 0,5 – 2,5 g/L. Nilai absorbansi turbiditas awal pada menit pertama berkisar antara 0,336 hingga 0,44, sedangkan absorbansi maksimum saat presipitasi maksimum berkisar antara 0,393 hingga 0,806, meningkat seiring dengan kenaikan konsentrasi Fe²⁺ dalam larutan. Setelah penarikan magnet dengan palladium ke dasar kuvet, absorbansi menurun dan stabil sejak menit kesembilan dengan rentang absorbansi 0,047 – 0,173. Absorbansi mencapai titik terendah pada menit keempat belas dengan rentang 0,002 – 0,03. Kurva standar yang dihasilkan dari selisih absorbansi turbiditas presipitat dan absorbansi supernatant setelah magnetisasi menunjukkan linearitas sebesar 0,94.
Hubungan Kadar Ferritin dengan Indeks Eritrosit Pada Penderita Anemia Defisiensi Besi Di Laboratorium Bio Medika Gandaria Tahun 2024 Susanti, Ellis; Latifah, Imas; Nurhidayat, Diki
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 10 No. 2 (2024): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v10i2.2480

Abstract

Anemia is a health problem with a high prevalence in Indonesia. Iron deficiency anemia, where a person's hemoglobin concentration is less than 95% of the mean value. Ferritin and MCV are sensitive parameters for iron deficiency anemia. This study aims to determine the relationship between Ferritin and MCV levels in patients with iron deficiency anemia. This research method is descriptive using secondary data at Bio Medika Gandaria Laboratory from January to June 2024 in 40 patients with Iron Deficiency Anemia (ADB). ADB patients are patients based on doctor's diagnosis on the patient's examination form. This study shows that the prevalence of Iron Deficiency Anemia patients of Female gender is higher as many as 32 patients (80%) with an average Ferritin level of 14.83 ng/mL with details of Low Ferritin levels as many as 21 patients (52.5%), Normal Ferritin 11 patients (27.5%), High Ferritin 0 patients (0.0%), and average MCV levels worth 71.70 fL with details of patients having Low MCV levels as many as 23 patients (57.5%), Normal MCV 9 patients (22.5%), High MCV 0 patients (0.0%). The results of the relationship test research using Pearson correlation, with a relationship coefficient value of p = 0.020, (p < 0.05), it can be concluded that there is a unidirectional relationship between Ferritin and MCV, the lower the Ferritin level, the lower the MCV level. Keywords: Anemia, Iron Deficiency Anemia, Ferritin, MCV
Sensitivitas Antara Antibiotik Meropenem dan Seftazidin Terhadap Pseudomonas aeruginosa Strain ATCC 15442 Pada Pasien Pneumonia di RSUP dr. Cipto Mangunkusumo Jakarta Pusat Diah Lestari; Husyain Djajaningrat; Febri Wulan Dari; Dean Handimulya
Anakes : Jurnal Ilmiah Analis Kesehatan Vol. 11 No. 1 (2025): ANAKES: Jurnal Ilmiah Analis Kesehatan
Publisher : Universitas Mohammad Husni Thamrin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37012/anakes.v11i1.2500

Abstract

Pneumonia is an acute lower respiratory tract infection in the lung parenchyma that has a high level of treatment resistance and is a nosocomial bacterial transmission. One of the nosocomial bacteria in hospitals is Pseudomonas aeruginosa, which is a Multi Drug Resistant (MDR) bacteria, a pathogen that causes infections that are resistant to at least three antimicrobial agents. The antimicrobials included are meropenem and ceftazidime, these two antibiotics are the best choice of last-line drugs in the treatment of MDR bacteria. Effective prevention and treatment of bacterial colonization and infection in pneumonia patients needs to be done to reduce the negative impact of antibiotic use. The aim of this study was to determine the sensitivity between the antibiotics meropenem and ceftazidime against Pseudomonas aeruginosa bacteria in pneumonia patients at RSUPN dr. Cipto Mangunkusumo, Central Jakarta. The research method was analytical observational using a cross sectional design with medical record data sources on 105 pneumonia patients who underwent sputum culture examination. Data analysis using the Mann-Whitney Test at 95% CI (α = 0.05). The research results showed that the majority of patients were male, 69 people with an elderly age category of 51 people (48.6%). The results of the antibiotic sensitivity of meropenem were 76.25% and ceftazidime were 69.5%, with a p-value of 0.000. The average sensitivity of the antibiotic meropenem is 20.59, more sensitive than the antibiotic ceftazidime, an average of 20.50 with a difference in sensitivity of 9%. The conclusion is that there is a difference in the sensitivity of the antibiotics meropenem and ceftazidime against Pseudomonas aeruginosa bacteria in pneumonia patients at RSUPN Dr. Cipto Mangunkusumo, Central Jakarta, the antibiotic meropenem is more sensitive than the antibiotic ceftazidime.  Keywords: Meropenem, Pneumonia, Pseudomonas aeruginosa,  Seftazidim.

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