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The Effectiveness of Lactobacillus rhamnosus Administration on the Growth of Klebsiella pneumoniae in Mice (Mus musculus) Ahmad, Izhar Fitrah; Wiriansya, Edward Pandu; Anggita, Dwi; Basri, Rezky Pratiwi Lambang; Sodiqah, Yani; Nurmadilla, Nesyana
Jurnal Respirasi Vol. 11 No. 2 (2025): May 2025
Publisher : Faculty of Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jr.v11-I.2.2025.147-152

Abstract

Introduction: Klebsiella pneumoniae is a bacterium from the Enterobacteriaceae family and is considered one of the most dangerous pathogens. Antibiotics are crucial for treating acute bacterial infections. Using probiotics has become one of the supportive and curative efforts in managing the growth of K. pneumoniae. One probiotic derived from microorganisms is Lactobacillus rhamnosus. This study aimed to determine the effectiveness of L. rhamnosus on the growth of K. pneumoniae in mice (Mus musculus). Methods: This was an experimental post-test study. The study population consisted of mice aged 3 to 4 months, weighing 20 to 40 grams, determined using Federer’s formula. Mice were given standard feed and sterile distilled water. A total of 30 mice were tested and divided into five treatment groups. The distribution colony count test was used for evaluation. Results: Among the five groups studied, probiotic intervention in the group receiving a combination of L. rhamnosus and the antibiotic ceftriaxone showed effective results (p<0.05), as did the group given only the probiotic L. rhamnosus (p<0.05). In contrast, the other groups did not show effective results (p>0.05). Conclusion: The administration of L. rhamnosus is effective as a supportive and curative treatment, but it is not effective as a preventive measure against the growth of K. pneumoniae in mice.
Strategi Komunikasi Efektif dalam Pendidikan Anak Usia Dini: Studi Kasus di TK An-Nizam Kristina Tobing, Hosanna; Gabriela Simanjuntak, Mulyani; Anggita, Dwi; Amanda, Dina; Siska Anggraini, Elya; Simare-mare , Aman
Jurnal Global Ilmiah Vol. 1 No. 10 (2024): Jurnal Global Ilmiah
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/jgi.v1i10.98

Abstract

Penelitian ini bertujuan untuk mengidentifikasi strategi komunikasi yang digunakan guru di TK An-Nizam, mengevaluasi efektivitas metode komunikasi tersebut, serta memahami tantangan dalam berkomunikasi dengan siswa. Metode penelitian yang digunakan adalah observasi, wawancara, dan survei terhadap guru, siswa, dan orang tua. Hasil penelitian menunjukkan bahwa penggunaan alat peraga visual dan media interaktif sangat membantu dalam menyampaikan materi pelajaran. Instruksi verbal yang jelas dan sederhana juga efektif dalam membantu pemahaman anak-anak. Partisipasi aktif siswa dalam kegiatan pembelajaran sangat didorong. Penelitian ini menyimpulkan bahwa komunikasi yang efektif sangat penting dalam pendidikan anak usia dini dan memberikan rekomendasi untuk meningkatkan penggunaan media interaktif serta keterlibatan orang tua dalam pendidikan anak.
Efektifitas Pemberian Bifidobacterium Longum Terhadap Pertumbuhan Klebsiella Pneumonia Pada Mencit Syarkawi, M. Fathir Faisal Thahir; Wiriansya, Edward Pandu; Murfat, Zulfitriani; Muchtar, Amrizal; Anggita, Dwi
MAHESA : Malahayati Health Student Journal Vol 5, No 8 (2025): Volume 5 Nomor 8 (2025)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v5i8.19120

Abstract

ABSTRACT This study aims to evaluate the effectiveness of Bifidobacterium longum administration on the growth of Klebsiella pneumoniae in mice (Mus musculus). A true experimental design with a post-test approach was employed. Mice were divided into five treatment groups: preventive, curative, supportive, positive control, and negative control. Bifidobacterium longum was administered orally at a dose of 10⁷ CFU/ml, while Klebsiella pneumoniae was inoculated intraperitoneally at a dose of 10⁶ CFU/ml. Bacterial colony counts were determined using blood cultures on MacConkey agar medium and a colony counter. The results showed that Bifidobacterium longum was ineffective in preventing Klebsiella pneumoniae growth in a preventive manner. However, Bifidobacterium longum demonstrated effectiveness in inhibiting Klebsiella pneumoniae after infection, making it a promising potential curative and supportive therapy. Bifidobacterium longum shows potential as a probiotic agent to inhibit Klebsiella pneumoniae infections, supporting the development of alternative and supportive therapies to combat infections and antibiotic resistance. Keywords: Bifidobacterium longum, Klebsiella pneumoniae, Probiotics, Antibacterial, Mice.  ABSTRAK Penelitian ini bertujuan untuk mengevaluasi efektivitas pemberian Bifidobacterium longum terhadap pertumbuhan Klebsiella pneumoniae pada mencit (Mus musculus). Kami menggunakan true experimental dengan desain post-test. Mencit dibagi menjadi lima kelompok perlakuan: preventif, kuratif, suportif, kontrol positif, dan kontrol negatif. Pemberian Bifidobacterium longum dilakukan secara oral dengan dosis 10⁷ CFU/ml, sedangkan Klebsiella pneumoniaediinokulasikan secara intraperitoneal dengan dosis 106CFU/ml. Jumlah koloni bakteri dihitung melalui kultur darah pada medium MacConkey menggunakan colony counter. Hasil menunjukkan bahwa Bifidobacterium longum tidak efektif dalam mencegah pertumbuhan klebsiella pneumoniae secara preventif. Namun Bifidobacterium longummenunjukkan efektivitas dalam menghambat klebsiella pneumoniae setelah infeksi terjadi, menjadikannya potensi terapi kuratif dan suportif yang menjanjikan. Bifidobacterium longum memiliki potensi sebagai agen probiotik untuk menghambat infeksi Klebsiella pneumoniae mendukung pengembangan terapi alternatif dan supportif untuk mengatasi infeksi dan resistensi antibiotik. Kata Kunci: Bifidobacterium Longum, Klebsiella Pneumoniae, Probiotics, Antibacterial, Mice.
HIV DENGAN TB PARU DAN PNEUMOCYSTIC JIROVECII PNEUMONIAE : LAPORAN KASUS Yusuf, Yuskyiah Ananda; Syahril, Erlin; Anggita, Dwi
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 9 No. 2 (2025): AGUSTUS 2025
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v9i2.48069

Abstract

Infeksi Human Immunodeficiency Virus (HIV) merupakan masalah kesehatan global yang menyebabkan penurunan sistem imun dan meningkatkan kerentanan terhadap infeksi oportunistik, seperti tuberkulosis (TB) dan Pneumocystis jirovecii pneumonia (PJP). Indonesia saat ini menjadi salah satu negara dengan beban TB tertinggi di dunia, dan koinfeksi HIV-TB menambah kompleksitas tatalaksana klinis. PJP juga menjadi infeksi oportunistik dengan mortalitas tinggi pada pasien HIV dengan imunosupresi berat. Penulisan ini bertujuan untuk mendeskripsikan proses diagnostik dan penatalaksanaan pada pasien dengan koinfeksi TB paru, HIV, dan PJP. Metode yang digunakan adalah laporan kasus deskriptif terhadap seorang perempuan berusia 39 tahun dengan gejala sesak napas, batuk berdahak, penurunan berat badan, dan hasil penunjang yang menunjukkan koinfeksi. Data diperoleh melalui anamnesis, pemeriksaan fisik menyeluruh, pemeriksaan laboratorium, radiologi (foto toraks dan CT-scan), serta pemeriksaan mikrobiologi dan imunologi. Diagnosis ditegakkan sebagai TB paru kasus baru, HIV reaktif dengan kadar CD4 27/μL, dan PJP berdasarkan temuan radiologis serta identifikasi kista Pneumocystis jirovecii. Penatalaksanaan diberikan secara multidisipliner menggunakan antibiotik, steroid, mukolitik, serta terapi suportif lainnya. Koinfeksi TB-HIV-PJP memperlihatkan hubungan patofisiologis yang saling memperburuk, menyebabkan penurunan imunitas lebih lanjut dan meningkatkan risiko kematian. Pemahaman mendalam tentang interaksi patogen dan penanganan tepat waktu sangat penting dalam memperbaiki prognosis pasien. Kasus ini menekankan pentingnya pendekatan holistik dan berbasis bukti dalam menangani pasien HIV dengan infeksi oportunistik multipel.
Kajian Kritik Sastra dengan Pendekatan Psikologi Sastra pada Cerpen Kucing Setan yang Merasakan Cinta Karya Cicilia Oday Anggita, Dwi; Satriani, Irma
DIDAKTIS : Jurnal Pendidikan Bahasa dan Sastra Indonesia Vol 3 No 2 (2025): DIDAKTIS: Jurnal Pendidikan Bahasa dan Sastra Indonesia
Publisher : Fakultas Sastra Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/didaktis.v3i2.924

Abstract

This study aims to reveal the psychological dynamics of the main character in the short story Kucing Setan yang Merasakan Cinta by Cicilia Oday through the lens of literary psychology using Sigmund Freud's psychoanalytic theory. The short story stands out by presenting a cat character as a symbolic figure undergoing an inner transformation influenced by its social environment. Employing a qualitative descriptive method with reading and note-taking techniques as well as literature review, this research analyzes the character's personality structure based on Freud's three main components: id, ego, and superego. The results show that the cat undergoes complex psychological conflicts, where instinctual drives (id) initially dominate its behavior filled with anger and vengeance. However, as the story progresses, the development of the ego enables the character to mediate between these instinctual urges and the social norms introduced by the environment. The superego emerges as a moral compass when the character begins to experience affection and comprehend the value of sacrifice. This transformation demonstrates that even symbolic non-human characters possess psychological structures worth exploring. This study is expected to contribute to Indonesian literary criticism by expanding the analytical space for symbolic characters in short stories.
FACTORS THAT INFLUENCE OWNERSHIP ON THE INCIDENCE OF DIARRHEA IN THE AREA OF PEMATANG CENGAL COMMUNITY HEALTH CENTER, LANGKAT REGENCY Nanda, Meutia; Anggita, Dwi; Khathimah, Husnul; Masyithah, Ruhul; Harahap, Dwi Adelia Putri
HEARTY Vol 13 No 5 (2025): OKTOBER
Publisher : Fakultas Ilmu Kesehatan, Universitas Ibn Khaldun, Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32832/hearty.v13i5.21523

Abstract

Diarrhea is a condition where a person defecates more than 3 times a day. Diarrhea is still one of the public health problems that is of concern because it is the third major contributor to child morbidity and mortality in various developing countries including Indonesia. This study aims to determine the effect of latrine sanitation variables and defecation behavior on the incidence of diarrhea in Pematang Cengal village. The research method is a quantitative study using an analytical survey design with a Cross-Sectional approach. From the total population obtained, 3452 residents were obtained samples received from Pemantang Cengal Village were 97 respondents using the slovin formula. This study utilized instruments using secondary data from the Pematang Cengal Village Health Center. Data were analyzed using SPSS to test the Univariate, Bivariate and Multivariate analysis hypotheses using the chi square statistical test at a significance level of 95% (p-Value <0.05). The results obtained from 6 research variables conducted, there are 5 variables that have a relationship, namely ownership of a decent toilet (0.001), the distance of the toilet is at least 10 m from the water source (0.001), the toilet has a cover (0.007), the toilet has a septic tank (0.000), and the behavior of defecating in a private toilet (0.007). While the variable that has no effect is the habit of washing hands after defecating has no effect (0.057> 0.005). The multivariate results show that the variable of ownership of a septic tank has the most influence on the incidence of diarrhea with an OR value = 1.56. The conclusion is that ownership of a decent toilet, the distance of the toilet is at least 10 m from the water source, the toilet has a cover, the toilet has a septic tank, and the behavior of defecating in a private toilet are behaviors that prevent the transmission of diarrhea in Pematang Cengal Village. Overall, this study emphasizes the importance of ownership and use of healthy toilets and a good sanitation system to prevent the incidence of diarrhea in the community.
Literatur Abortus Dan Penanganannya Irawawan, Dian Nirwana; Niken, Niken; sandi, Elmatiana dara; Anggita, Dwi
Stetoskop: The Journal Of Health Science Vol. 1 No. 2 (2024): December 2024
Publisher : CV. Cendikiawan Muda Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70656/stjhs.v1i2.167

Abstract

Abortus merupakan salah satu permasalahan kesehatan reproduksi yang signifikan, terutama di negara berkembang. Artikel ini bertujuan untuk mengulas faktor penyebab, dampak, serta metode penanganan abortus berdasarkan literatur terkini dan pendapat ahli. Penyebab abortus meliputi faktor medis, sosial, dan psikologis, yang masing-masing memerlukan pendekatan khusus. Dampak abortus tidak hanya bersifat fisik tetapi juga psikologis dan sosial. Dengan pendekatan medis yang tepat dan dukungan sosial, penanganan abortus dapat dioptimalkan. Artikel ini diakhiri dengan kesimpulan yang menekankan pentingnya edukasi dan intervensi dini dalam mencegah serta menangani abortus.