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All Journal Alchemy : Journal of Chemistry Indonesian Journal of Cancer Chemoprevention INDONESIAN JOURNAL OF PHARMACY Journal of Islamic Pharmacy Science and Technology Indonesia Jurnal Farmasi Sains dan Komunitas Journal of Islamic Medicine Pharmacology and Clinical Pharmacy Research Jurnal Tumbuhan Obat Indonesia
Roihatul Mutiah
Universitas Islam Negeri Maulana Malik Ibrahim Malang
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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Compound Identification and Anticancer Activity of Ethyl Acetate Fraction from Bawang Sabrang (Eleutherine palmifolia (L.) Merr.) on HeLa Cervical Cancer Cell Line Mutiah, Roihatul; Minggarwati, Trian Sidha; Kristanti, Risma Aprinda; Susanti, Erna
Indonesian Journal of Cancer Chemoprevention Vol 10, No 3 (2019)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev10iss3pp131-139

Abstract

Eleutherine palmifolia (L.) Merr. is a typical plant found in Central Kalimantan that has been used empirically by the Dayak people as medicine for various diseases, including cancer. The plant contains flavonoid compounds that potentially used as an anticancer. The purpose of this study is to find the most active fraction, indicated by its cytotoxic potency on HeLa cervical cancer cell line, and to identify compounds in E. palmifolia bulbs fraction. E. palmifolia bulbs was extracted by maceration. The extraction with ultrasonic bath and partition fractionation was conducted by using n-hexane, chloroform, and ethyl acetate. Each fraction was tested for toxicity level on HeLa cells using MTT assay. The identification of active compounds was carried out by Ultra Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS). The result showed that based on the IC50 value, the ethyl acetate fraction had the highest bioactivity. IC50 values of n-hexane, chloroform, and ethyl acetate fractions were 250.77±19.01; 720.46±42.38; and 44.34±9.45μg/mL, respectively. The identification of the active compound in ethyl acetate fraction resulted 28 chemical compounds. Compounds with the highest percentage area were isoliquiritigenin and oxyresveratrol. The ethyl acetate fraction of E. palmifolia bulbs is potential to be developed as an anticancer candidate (phytopharmaceutical).Keywords: Compound identification, Anticancer activity, Eleutherine palmifolia (L.) Merr., cervical cancer
Induction of Helianthus annuus Leaves Extract to HeLa cell Apoptosis and Cell Cycle Arrest in S, G2-M and M5 Phase Mutiah, Roihatul; Ulfah, Jauza; Amrulloh, Muhammad Firman; Suryadinata, Arief; Indrawijaya, Yen Yen Ari; Rahmawati, Ana
Indonesian Journal of Cancer Chemoprevention Vol 13, No 1 (2022)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev13iss1pp1-11

Abstract

Helianthus annuus L. (H. annuus) is a potential medicinal plant for cancer therapy. The aims of this study is to identify profile the anticancer activity of H. annuus L. from its leaves, root, stem, and seed as well as to elucidate the apoptosis and cell cycle of the leaves. Ten-gram sample of the powder were extracted by using Ultrasound-Assisted Extraction (UAE) with 200 ml of 96% ethanol by comparison of 1:20 with three times replications. The determination of anticancer activity was used the MTT cell proliferation assay, while apoptosis test and cell cycle were applied with the flowcytometry test. The value of IC50 in 96% ethanol extract in the root and stem was >1,000 μg/mL; seed and leaves were 153.76 μg/mL; and 126.6 μg/mL, respectively. The apoptosis induction of H. annuus leaves extract treatment was 7.17% of apoptosis cells; 90.44% of necrosis, and 2.39% of living cells. The H. annuus leaves extract also significantly caused a decrease of cell percentage in G0-G1 phase (p<0.001) and an increase in G2-M phase (p<0.001). The H. annuus leaves extract had greater potential as anticancer instead of other parts. The adding of H. annuus leaves extract increased the HeLa cell apoptosis, decreased percentage of HeLa cells in G0-G1 phase, and increased percentage of HeLa cells in G2-M phase. Cell cycle mechanism test showed cell cycle arrest in S, G2-M, and M5 phase in 24 h, hence inhibited the mitosis process.Keywords: anticancer, Helianthus annuus L, apoptosis, cell cycle.
Co-Authors Abdul Hakim Ach. Nashichuddin Ahmad, Ananda Rizkia Azizah Amrulloh, Muhammad Firman Andiartama, Amanda Milano Anik Listiyana Anik Listyana Annisa, Rahmi Arief Suryadinata Aty Widyawaruyanti Begum Fauziyah Briliana, Malich Septi Diajeng Dhani Wijaya, Dhani Elok Kamilah Hayati Erna Susanti Fathia Faza Rahmadanita Ferry Sandra Habibah, Atiqoh Nur Hadya, Chorida Muhjatul Hariz, Muhammad Fawaz Huwaida, Fadhila Isma Indradewa, Rhian Irawati, Santia Janaloka, Nandana Adyuta Kirana, Farenza Okta Laily Nurul Azizah Lestari, Nia Ayu Ma'arif Z.A, Burhan Ma'arif, Burhan Ma'arif, Burhan Minggarwati, Trian Sidha Muhammad Zainuddin Mustofa, Muhammad Ragib Nafisah, Fika Sayyidatun Nurlaili Susanti Octavia, Nur Ika Purwaningsih, Fauziyah Eni Qonita, Kesimira Rahmi Annisa Ramadhani D.A., Ria Retno Susilowati Ria Ramadhani Dwi Atmaja Risma Aprinda Kristanti Sadli Syarifuddin Salsabila, Dhia Fadhilah Salsabilla, Farida Rahma Samah, Moh.Alfin Siti Zulaikah Sukardiman Sukma, Yolanda Cahyaning Suyadinata, Arief Tanaya Jati Dharma Dewi Taufik, Imam Ulfah, Jauza Weka Sidha Bhagawan Wirda Anggraini Yen Yen Ari Indrawijaya Zahiro, Syayida Roisatus