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Efektivitas Ekstrak Daun Patikan Kebo (Euphorbia hirta L.) Dibandingkan dengan Povidone Iodine 10% terhadap Ketebalan Epitelisasi pada Luka Insisi Tikus Putih Jantan Nurbaiti Nurbaiti; Ari Fajar Ramdani; Hikmah Fitriani
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 4, No 1 (2018): Tunas Medika Jurnal Kedokteran & kesehatan
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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ABSTRAK Latar Belakang: Luka insisi yaitu adanya robekan linier pada kulit dan jaringan di bawahnya karena teriris oleh instrumen tajam. Penyembuhan luka dapat dibagi ke dalam tiga fase, yaitu fase inflamasi, proliferasi, dan remodelling. Epitelisasi mulai terbentuk pada fase proliferasi. Ekstrak daun patikan kebo (Euphorbia hirta L.) mengandung tanin, saponin, dan senyawa flavonoid yang berperan dalam proses penyembuhan luka yang melibatkan proses epitelisasi.Tujuan : Mengetahui perbandingan efektivitas ekstrak daun patikan kebo (Euphorbia hirta L.) dibandingkan dengan povidone iodine terhadap ketebalan epitelisasi pada luka insisi tikus putih jantan.Metode : Penelitian ini adalah penelitian eksperimental dengan rancangan post test only control group design dengan menggunakan hewan percobaan tikus putih jantan galur wistar sebagai subjek penelitian. Penelitian ini menggunakan lima kelompok yaitu satu kelompok kontrol negatif, satu kelompok kontrol positif menggunakan povidone iodine 10%, dan tiga kelompok perlakuan menggunakan dosis 2,75%, 5,50% dan 8,25%. Uji statistik menggunakan One Way ANOVA.Hasil : Hasil penelitian menunjukkan bahwa ekstrak daun patikan kebo (Euphorbia hirta L.) dengan dosis 8,25% lebih efektif dibandingkan povidone iodine 10% sebesar 66.360 µm (P<0.05).Kesimpulan : Pemberian ekstrak daun patikan kebo (Euphorbia hirta L.) terhadap proses ketebalan epitelisasi pada luka insisi lebih efektif dibandingkan dengan povidone iodineKata Kunci : Ketebalan epitelisasi, luka insisi, patikan kebo, povidone iodine 10%

Pengaruh Ekstrak Daun Annona muricata L. Terhadap Ekspresi Caspase 3 Pada Adenokarsinoma Mamma Mencit C3H Hikmah Fitriani; Ruri Eka Maryam; Tissa Octavira Permatasari
Tunas Medika Jurnal Kedokteran & Kesehatan Vol 2, No 1 (2015): Tunas Medika Jurnal Kedokteran & Kesehatan
Publisher : Tunas Medika Jurnal Kedokteran & Kesehatan

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Background :Treatment of breast cancer such as surgery, radiotherapy and chemotherapy still has a lot of sideeffects. This can be minimized by using herbal medicine such as Annona muricata L. which works selectively attacking cancer cells.Objective :The aim for this research was to determine effect of Annona muricata L. leaves extract on the caspase 3 expression on adenocarcinoma mammae in C3H mice.Methods : Animalexperiment using Post Test Only Control Group design was done. Twelve C3H mice were inoculted with tumour and were devided into two groups : Control (C) group and the other group was Treatment (T) group with Annona muricata L. leaves extract of dose 1 mg/day. All of the groups were treated for three weeks and then measuring caspase 3 expressi on. Mann Whitney test was conducted to analyze the data.Result : There were significant differences on the caspase 3 expression between control group compared with one group administrated with Annona muricata L. leaves extract of dose 1 mg/day respectively ( p = 0,004) between control group compared with one group administrated with Annona muricata L. leaves extract of dose 1 mg/day( p = 0,004). From the result, median of caspase 3 expression were increased with Control = 0,4 and Treatment = 1,36.Conclution :Annona muricata L. leaves extract can increase caspase 3 expressionKeywords:Annona muricata L. leaves extract, caspase 3, adenocarcinoma mammae

THE EFFECTIVENESS OF LEMON JUICE (Citrus limon) TOWARD PYRAMIDAL NEURON CELLS OF MALE WHITE MICE’SCEREBRAL CORTEX THAT EXPOSED BY MONOSODIUM GLUTAMATE (MSG) Pepi Arifiyani; Hikmah Fitriani; Rachmanda Haryo Wibisono
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: The consumption rate of Monosodium Glutamate (MSG) in Indonesia remains high. Lemon is an antioxidant and neutralizer of free radicals which contains vitamin C and phytochemicals to prevent the long-term effects of MSG to pyramida cells. This study aims to test the effect of oral administration of lemon juice (Citrus limon) to the number of pyramidal neuron cells. Methodology: This experimental study employed 30 male white mice aged 2.5 to 3 months. Those mice were randomly divided into five control-groups: Normal Control (KN), Negative Control (K-) was treated with 4 mg/grBB MSG, Dose Group 1 (KD1) was treated with 3.33 ml/kgBB lemon juice, Dose Group 2 (KD2) was treated with 6.67 ml/kgBB lemon juice, and Dose Group 3 (KD3) was treated with 13.33 ml/kgBB lemon juice. Kruskal Wallis test was used to determine the difference in distribution between groups, followed with Post hoc Mann Whitney test.Results: After 28 days of treatment, the average number of pyramidal neuron cells in dose group 1 (637 cells) was higher than in negative control (589.5 cells) (p=0.078). The average number of pyramidal neuron cells in dose group 3 (482,5 cells) was lower than in the negative control (589.5 cells) (p=0.016). The statistical result shown that there were groups with a significant difference in pyramidal cells number. Mann-Whitney analysis of pyramidal cells number had shown that there was a significant difference between group KD3 and KD1, KD3 and K (-), KD3 and KD2.Conclusion: Lemon juice (Citrus limon) in precise dose has a beneficial effect to reduce the excitotoxic effect of MSG. Further studies is needed to validate the active compound and toxicity effect of lemon juice.

INFLUENCE OF GIVING BREADFRUIT (Artocarpus altilis (Park) Fosberg.) LEAVES EXTRACT TO DECREASE URIC ACID LEVELS IN WISTARS RATS HYPERURICEMIC Hagi Wibawa; Hikmah Fitriani; Rama Samara B; Irwan Meidi L
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Hyperuricemic is a condition where the uric acid levels in blood more than 7 mg/dl. In 2010, Hyperuricemic was included as one of non-communicable disease with incidence rate up to 30% and can cause the mortality. This study aims to examine the effective dose of breadfruit (Artocarpus altilis (Park) Fosberg.) leaves extract to decrease uric acid levels in Wistar rats Hyperuricemic.Methodology: This study was an experimental with pre-posttest with control group design. 30 Wistar rats aged three months was divided into 2 control groups (K1 and K2) and 3 treatment groups (K3, K4, and K5). Control groups were given purine (K1= standard, K2=high) and aquadest. Differently, treatment groups were given high purine and breadfruit leaf extract (K3=0.25g /KgBW, K4=0.5g /KgBW, K5=0.75g/KgBW). All the treatments were given one time daily in the morning for 7 days. Data were analyzed by the Shapiro-Wilk test followed by the One-Way ANOVA test and then Post-hoc Tamhane test.Result: The control groups revealed negative average reduction of uric acid in mg/dl (K1= -0.05, K2= -0.09) whereas the treatment groups revealed the positive average reduction of uric acid in mg/dl (K3=2.19, K4= 4.26, K5=5.87). The significant differences (p<0.05) of uric acid levels before and after treatment in each treatment groups had meaning that giving the Breadfruit leaf extract could reduce the uric acid level rats. Post-hoc Tamhane test result showed that the mean decrease of each group was significantly different. Particularly, K5 was the most effective treatment compared with others. Conclusion: Breadfruit leaves extract with dose of 0.75g/KgBW was the most effective treatment to reduce the uric acid level in blood. Stakeholders in health field should promote the Breadfruit as the traditional medicine method to reduce the uric acid in blood.

THE EFFECTIVENESS OF LEMON JUICE (Citrus limon) ON PURKINJE CELL OF WHITE MALE MICE (Mus musculus) CEREBELLAR CORTEX THAT EXPOSED BY MONOSODIUM GLUTAMATE (MSG) I Gusti Ayu Novita Afsari; Hikmah Fitriani; Triono Adi Suroso
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Introduction: A number of studies have indicated that excessive MSG (Monosodium glutamate) consumption can lead to the formation of free radicals that can have a negative effect on purkinje cells in the cerebellar cortex. Lemon fruit is a plant that has benefits as a natural antioxidant because it contains compounds such as flavonoids, vitamin C, citric acid and other substances. This study aims to examine the effect of lemon juice (Citrus limon) to purkinje cells of the cerebellum cortex on male white mice (Mus musculus) which exposed of monosodium glutamate.Method: This research is an experimental laboratory with a post test only control group design. The research subjects were 30 white male mice divided into 5 groups: normal control given standard feed, negative control given MSG dose 4mg / grBW, group of dose I , dose II , and dose III that were each given lemon juice with doses 3.33ml / kgBW, 6.67ml / kgBW, 13.33ml/ kgBW respectively. The number of purkinje cells is calculated in the cerebellar cortex by HE staining. The results of the study were analyzed by One Way ANOVA followed by the Bonferroni Post hoc test.Results: The average number of purkinje cells in administration of lemon juice with dosages of 3.33 ml/kgBW, 6.67 ml/kgBW and 13.33 ml /kgBW were 14.10 cells, 16.73 cells and 17.50 cells respectively. Based on the Post Hoc test, the average number of purkinje cells at the dose of 13.33 ml / kgBW was higher than the negative control (p = 0.021)Conclusion: Lemon juice could be used to reduce negative effect of MSG on purkinje cells of white male mice. Moreover, this finding could be used as reference on further research of benefit of lemon juice.

THE INHIBITION TEST OF FLAVONOID AND TANNIN IN NONI FRUIT (Morinda citrifolia Linn) COMPARED TO CEFTRIAXONE TOWARDS Escherichia coli IN VITRO Tessa Swesty Islamia; Atik Sutisna; Hikmah Fitriani
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: Escherichia coli is one of the causatives of infection agents. Noni fruit that contains the antibacterial ingredients was predicted as an alternative medicine to reduce the resistance of antibiotics. Noni fruit extract can inhibit the growth of Escherichia coli. The absence of research on the active substances contained in this fruit which plays a role in inhibiting the growth of Escherichia coli encourages the researcher to conduct this research.Methodology: The research was an experimental laboratory with post-test only control group design. The Escherichia coli grown on Mac conkey agar. Flavonoid and tannin in noni fruit (Morinda citrifolia Linn.) diluted with 3 concentrations, 50%, 70%, 100% and 2 control groups, positive control with ceftriaxone and negative control with Dimethylsulfoxide 10% (DMSO). The treatment group were incubated for 24 hours, then the inhibitory zone formed was measured in mm. Results: The Kruskall-Wallis test showed significant difference in all concentration. The results obtained from this research showed that the average of inhibition towards Escherichia coli on positive control (22.83 mm) had the largest inhibition zone among flavonoid 100% (18.83mm), flavonoid 70% (14.67 mm), flavonoid 50% (6 mm), tannin 100% (17.33 mm), tannin 70% (10.83 mm), tannin 50% (7.50 mm), and negative control (2 mm).Conclusion: Flavonoid and tannin in noni fruit (Morinda citrifolia Linn) were effective for inhibiting the growth of Escherichia coli. The 100% concentration is the most effective concentration that can be promoted as an alternative medicine.

THE EFFECTIVENESS OF LEMON JUICE (Citrus limon) ON PURKINJE CELL OF WHITE MALE MICE (Mus musculus) CEREBELLAR CORTEX THAT EXPOSED BY MONOSODIUM GLUTAMATE (MSG) I Gusti Ayu Novita Afsari; Hikmah Fitriani; Triono Adi Suroso
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Introduction: A number of studies have indicated that excessive MSG (Monosodium glutamate) consumption can lead to the formation of free radicals that can have a negative effect on purkinje cells in the cerebellar cortex. Lemon fruit is a plant that has benefits as a natural antioxidant because it contains compounds such as flavonoids, vitamin C, citric acid and other substances. This study aims to examine the effect of lemon juice (Citrus limon) to purkinje cells of the cerebellum cortex on male whitemice (Mus musculus) which exposed of monosodium glutamate. Method: This research is an experimental laboratory with a post test only control group design. The research subjects were 30 white male mice divided into 5 groups: normal control given standard feed, negative control given MSG dose 4mg / grBW, group of dose I , dose II , and dose III that were each given lemon juice with doses 3.33ml / kgBW, 6.67ml / kgBW, 13.33ml/ kgBW respectively. The number of purkinje cells is calculated in the cerebellar cortex by HE staining. The results of the study wereanalyzed by One Way ANOVA followed by the Bonferroni Post hoc test. Results: The average number of purkinje cells in administration of lemon juice with dosages of 3.33 ml/kgBW, 6.67 ml/kgBW and 13.33 ml /kgBW were 14.10 cells, 16.73 cells and 17.50 cells respectively. Based on the Post Hoc test, the average number of purkinje cells at the dose of 13.33 ml / kgBW was higher than the negative control (p = 0.021) Conclusion: Lemon juice could be used to reduce negative effect of MSG on purkinje cells of white male mice. Moreover, this finding could be used as reference on further research of benefit of lemon juice. Keyword: Citrus limon, purkinje cell, monosodium glutamate (MSG)

THE EFFECTIVENESS OF LEMON JUICE (Citrus limon) TOWARD PYRAMIDAL NEURON CELLS OF MALE WHITE MICE’SCEREBRAL CORTEX THAT EXPOSED BY MONOSODIUM GLUTAMATE (MSG) Pepi Arifiyani; Hikmah Fitriani; Rachmanda Haryo Wibisono
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

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Background: The consumption rate of Monosodium Glutamate (MSG) in Indonesia remains high. Lemon is an antioxidant and neutralizer of free radicals which contains vitamin C and phytochemicals to prevent the long-term effects of MSG to pyramida cells. This study aims to test the effect of oral administration of lemon juice (Citrus limon) to the number of pyramidal neuron cells. Methodology: This experimental study employed 30 male white mice aged 2.5 to 3 months. Those mice were randomly divided into five control-groups: Normal Control (KN), Negative Control (K-) was treated with 4 mg/grBB MSG, Dose Group 1 (KD1) was treated with 3.33 ml/kgBB lemon juice, Dose Group 2 (KD2) was treated with 6.67 ml/kgBB lemon juice, and Dose Group 3 (KD3) was treated with 13.33 ml/kgBB lemon juice. Kruskal Wallis test was used to determine the difference in distribution between groups, followed with Post hoc Mann Whitney test. Results: After 28 days of treatment, the average number of pyramidal neuron cells in dose group 1 (637 cells) was higher than in negative control (589.5 cells) (p=0.078). The average number of pyramidal neuron cells in dose group 3 (482,5 cells) was lower than in the negative control (589.5 cells) (p=0.016). The statistical result shown that there were groups with a significant difference in pyramidal cells number. Mann-Whitney analysis of pyramidal cells number had shown that there was a significant differencebetween group KD3 and KD1, KD3 and K (-), KD3 and KD2. Conclusion: Lemon juice (Citrus limon) in precise dose has a beneficial effect to reduce the excitotoxic effect of MSG. Further studies is needed to validate the active compound and toxicity effect of lemon juice. Keywords: Pyramidal neuron cells, monosodium glutamate, lemon (Citrus limon)

INFLUENCE OF GIVING BREADFRUIT (Artocarpus altilis (Park) Fosberg.) LEAVES EXTRACT TO DECREASE URIC ACID LEVELS IN WISTARS RATS HYPERURICEMIC Hagi Wibawa; Hikmah Fitriani; Rama Samara; Irwan Meidi L
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Hyperuricemic is a condition where the uric acid levels in blood more than 7 mg/dl. In 2010, Hyperuricemic was included as one of non-communicable disease with incidence rate up to 30% and can cause the mortality. This study aims to examine the effective dose of breadfruit (Artocarpus altilis (Park) Fosberg.) leaves extract to decrease uric acid levels in Wistar rats Hyperuricemic. Methodology: This study was an experimental with pre-posttest with control group design. 30 Wistar rats aged three months was divided into 2 control groups (K1 and K2) and 3 treatment groups (K3, K4, and K5). Control groups were given purine (K1= standard, K2=high) and aquadest. Differently, treatment groups were given high purine and breadfruit leaf extract (K3=0.25g /KgBW, K4=0.5g /KgBW, K5=0.75g/KgBW). All the treatments were given one time daily in the morning for 7 days. Datawere analyzed by the Shapiro-Wilk test followed by the One-Way ANOVA test and then Post-hoc Tamhane test. Result: The control groups revealed negative average reduction of uric acid in mg/dl (K1= -0.05, K2= -0.09) whereas the treatment groups revealed the positive average reduction of uric acid in mg/dl (K3=2.19, K4= 4.26, K5=5.87). The significant differences (p<0.05) of uric acid levels before and after treatment in each treatment groups had meaning that giving the Breadfruit leaf extract could reduce the uric acid level rats. Post-hoc Tamhane test result showed that the mean decrease of each group wassignificantly different. Particularly, K5 was the most effective treatment compared with others. Conclusion: Breadfruit leaves extract with dose of 0.75g/KgBW was the most effective treatment to reduce the uric acid level in blood. Stakeholders in health field should promote the Breadfruit as the traditional medicine method to reduce the uric acid in blood. Keywords: Breadfruit leaves, uric acid, Hyperuricemic, Wistar rats, flavonoids

THE INHIBITION TEST OF FLAVONOID AND TANNIN IN NONI FRUIT (Morinda citrifolia Linn) COMPARED TO CEFTRIAXONE TOWARDS Escherichia coli IN VITRO Tessa Swesty Islamia; Atik Sutisna; Hikmah Fitriani
Proceedings of the International Conference on Applied Science and Health No. 4 (2019)
Publisher : Yayasan Aliansi Cendekiawan Indonesia Thailand (Indonesian Scholars' Alliance)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Background: Escherichia coli is one of the causatives of infection agents. Noni fruit that contains the antibacterial ingredients was predicted as an alternative medicine to reduce the resistance of antibiotics. Noni fruit extract can inhibit the growth of Escherichia coli. The absence of research on the active substances contained in this fruit which plays a role in inhibiting the growth of Escherichia coli encourages the researcher to conduct this research. Methodology: The research was an experimental laboratory with post-test only control group design. The Escherichia coli grown on Mac conkey agar. Flavonoid and tannin in noni fruit (Morinda citrifolia Linn.) diluted with 3 concentrations, 50%, 70%, 100% and 2 control groups, positive control with ceftriaxone and negative control with Dimethylsulfoxide 10% (DMSO). The treatment group were incubated for 24 hours, then the inhibitory zone formed was measured in mm. Results: The Kruskall-Wallis test showed significant difference in all concentration. The results obtained from this research showed that the average of inhibition towards Escherichia coli on positive control (22.83 mm) had the largest inhibition zone among flavonoid 100% (18.83mm), flavonoid 70% (14.67 mm), flavonoid 50% (6 mm), tannin 100% (17.33 mm), tannin 70% (10.83 mm), tannin 50% (7.50 mm), and negative control (2 mm). Conclusion: Flavonoid and tannin in noni fruit (Morinda citrifolia Linn) were effective for inhibiting the growth of Escherichia coli. The 100% concentration is the most effective concentration that can be promoted as an alternative medicine. Keywords: Noni fruit, Flavonoid, Tannin, Escherichia coli, Inhibition test

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